ELF4 (NM_001421) Human Tagged ORF Clone

CAT#: RC200479

ELF4 (Myc-DDK-tagged)-Human E74-like factor 4 (ets domain transcription factor) (ELF4), transcript variant 1

Product Images

Circular map for RC200479

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ELF4 (Cat# RC200479, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ELF4 (Cat# TA810090)(1:2000). Positive lysates LY400550 (100ug) and LC400550 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY426701) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226077 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ELF4
• Synonyms: ELFR; MEF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200479 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTATTACCCTACAGCCCAGTGACCTGATCTTTGAGTTCGCAAGCAACGGGATGGATGATGATATCC
  ACCAGCTGGAAGACCCCTCTGTGTTCCCAGCTGTGATCGTGGAGCAGGTACCCTACCCTGATTTACTGCA
  TCTGTACTCGGGACTGGAGTTGGACGACGTTCACAATGGCATCATAACAGACGGGACCTTGTGCATGACG
  CAGGATCAGATCCTGGAAGGCAGTTTTTTGCTGACAGATGACAATGAGGCCACCTCGCACACCATGTCAA
  CCGCGGAAGTCTTACTCAATATGGAGTCTCCCAGCGATATCCTGGATGAGAAGCAGATCTTCAGTACCTC
  CGAAATGCTTCCAGACTCGGACCCTGCACCAGCTGTCACTCTGCCCAACTACCTGTTTCCTGCCTCTGAG
  CCCGATGCCCTGAACAGGGCGGGTGACACTAGTGACCAGGAGGGGCATTCTCTGGAGGAGAAGGCCTCCA
  GAGAGGAAAGTGCCAAGAAGACTGGGAAATCAAAGAAGAGAATCCGGAAGACCAAGGGCAACCGAAGTAC
  CTCACCTGTCACTGACCCCAGCATCCCCATTAGGAAGAAATCAAAGGATGGCAAAGGCAGCACCATCTAT
  CTGTGGGAGTTCCTCCTGGCTCTTCTGCAAGACAGAAACACCTGTCCCAAGTACATCAAGTGGACCCAGC
  GAGAGAAAGGCATCTTCAAACTGGTGGACTCCAAAGCTGTGTCCAAGCTGTGGGGGAAGCAGAAAAACAA
  GCCTGACATGAACTATGAGACAATGGGGCGGGCACTAAGATACTACTACCAAAGAGGCATACTGGCCAAA
  GTGGAAGGGCAGAGGCTGGTGTACCAGTTTAAGGAGATGCCCAAGGACCTGGTGGTCATTGAAGATGAGG
  ATGAGAGCAGCGAAGCCACAGCAGCCCCACCTCAGGCCTCCACGGCCTCTGTGGCCTCTGCCAGTACCAC
  CCGGCGAACCAGCTCCAGGGTCTCATCCAGATCTGCCCCCCAGGGCAAGGGCAGCTCTTCTTGGGAGAAG
  CCAAAAATTCAGCATGTCGGTCTCCAGCCATCTGCGAGTCTGGAATTGGGACCGTCGCTAGACGAGGAGA
  TCCCCACTACCTCCACCATGCTCGTCTCTCCAGCAGAGGGCCAGGTCAAGCTCACCAAAGCTGTGAGTGC
  ATCTTCAGTGCCCAGCAACATCCACCTAGGAGTGGCCCCCGTGGGGTCGGGCTCGGCCCTGACCCTGCAG
  ACGATCCCACTGACCACGGTGCTGACCAATGGGCCTCCTGCCAGTACTACTGCTCCCACTCAGCTCGTTC
  TCCAGAGTGTTCCAGCGGCCTCTACTTTCAAGGACACCTTCACTTTGCAGGCCTCTTTCCCCCTGAACGC
  CAGTTTCCAAGACAGCCAGGTGGCAGCCCCAGGGGCTCCACTGATTCTCAGTGGCCTCCCCCAACTTCTG
  GCTGGGGCCAACCGTCCGACCAACCCGGCGCCACCCACGGTCACAGGGGCTGGACCAGCAGGGCCCAGCT
  CTCAGCCCCCTGGGACTGTCATTGCTGCCTTCATCAGGACTTCTGGCACTACAGCAGCCCCTAGGGTCAA
  GGAGGGGCCACTGAGGTCCTCCTCCTATGTTCAGGGTATGGTGACGGGGGCCCCCATGGAGGGGCTGCTG
  GTTCCTGAAGAGACCCTGAGGGAGCTCCTGAGAGATCAGGCTCATCTTCAGCCACTTCCAACCCAGGTGG
  TTTCCAGGGGTTCCCACAATCCGAGCCTTCTGGGCAACCAGACTTTGTCTCCTCCCAGCCGCCCCACTGT
  TGGGCTGACCCCAGTGGCTGAACTTGAGCTCTCCTCAGGCTCAGGGTCCCTGCTGATGGCTGAGCCTAGT
  GTGACCACATCTGGGAGCCTTCTGACAAGATCCCCCACCCCAGCCCCTTTCTCCCCATTCAACCCTACTT
  CCCTCATTAAGATGGAGCCCCATGACATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200479 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAITLQPSDLIFEFASNGMDDDIHQLEDPSVFPAVIVEQVPYPDLLHLYSGLELDDVHNGIITDGTLCMT
  QDQILEGSFLLTDDNEATSHTMSTAEVLLNMESPSDILDEKQIFSTSEMLPDSDPAPAVTLPNYLFPASE
  PDALNRAGDTSDQEGHSLEEKASREESAKKTGKSKKRIRKTKGNRSTSPVTDPSIPIRKKSKDGKGSTIY
  LWEFLLALLQDRNTCPKYIKWTQREKGIFKLVDSKAVSKLWGKQKNKPDMNYETMGRALRYYYQRGILAK
  VEGQRLVYQFKEMPKDLVVIEDEDESSEATAAPPQASTASVASASTTRRTSSRVSSRSAPQGKGSSSWEK
  PKIQHVGLQPSASLELGPSLDEEIPTTSTMLVSPAEGQVKLTKAVSASSVPSNIHLGVAPVGSGSALTLQ
  TIPLTTVLTNGPPASTTAPTQLVLQSVPAASTFKDTFTLQASFPLNASFQDSQVAAPGAPLILSGLPQLL
  AGANRPTNPAPPTVTGAGPAGPSSQPPGTVIAAFIRTSGTTAAPRVKEGPLRSSSYVQGMVTGAPMEGLL
  VPEETLRELLRDQAHLQPLPTQVVSRGSHNPSLLGNQTLSPPSRPTVGLTPVAELELSSGSGSLLMAEPS
  VTTSGSLLTRSPTPAPFSPFNPTSLIKMEPHDI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001421
• ORF Size: 1989 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001421.4
• RefSeq Size: 4195 bp
• RefSeq ORF: 1992 bp
• Locus ID: 2000
• UniProt ID: Q99607
• Cytogenetics: Xq26.1
• Domains: ETS
• Protein Families: Transcription Factors
• MW: 70.7 kDa
• Gene Summary: The protein encoded by this gene is a transcriptional activator that binds and activates the promoters of the CSF2, IL3, IL8, and PRF1 genes. The encoded protein is involved in natural killer cell development and function, innate immunity, and induction of cell cycle arrest in naive CD8+ cells. Two transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Jan 2010]