KBTBD10 (KLHL41) (NM_006063) Human Tagged ORF Clone

CAT#: RC200295

KLHL41 (Myc-DDK-tagged)-Human kelch repeat and BTB (POZ) domain containing 10 (KBTBD10)

Product Images

Circular map for RC200295

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  Western blot validation of overexpression lysate (Cat# LY416896) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200295 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified KLHL41 protein (Cat# TP300295). The protein was produced from HEK293T cells transfected with KLHL41 cDNA clone (Cat# RC200295) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KBTBD10
• Synonyms: KBTBD10; Krp1; SARCOSIN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200295 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTCCCAGCGGGAACTTGCAGAGGAACTGCGGCTTTACCAATCCACCCTTCTTCAGGATGGTCTAA
  AAGATCTCCTGGATGAGAAAAAATTCATCGATTGCACCCTAAAAGCAGGTGACAAAAGTCTTCCTTGCCA
  CAGATTGATTTTGTCAGCTTGTAGTCCTTACTTCCGTGAGTACTTTTTATCTGAAATTGATGAGGCGAAA
  AAAAAGGAGGTAGTGCTAGACAATGTGGATCCTGCTATACTTGATTTAATCATCAAATACCTGTACTCTG
  CCAGTATTGATCTCAATGACGGAAATGTGCAAGATATTTTTGCATTGGCCAGCCGCTTTCAGATCCCCTC
  AGTGTTTACTGTCTGCGTTTCTTATCTTCAGAAAAGACTTGCTCCTGGTAACTGTCTAGCCATCCTAAGA
  TTAGGACTTCTTCTTGACTGCCCGAGACTCGCCATTTCTGCCCGTGAATTTGTGTCTGATCGCTTTGTAC
  AGATTTGTAAGGAAGAGGACTTTATGCAACTGTCTCCACAGGAACTGATCTCAGTCATTTCAAATGACAG
  CCTAAATGTAGAAAAAGAAGAAGCAGTATTTGAGGCAGTGATGAAATGGGTGCGAACAGACAAGGAAAAC
  AGGGTTAAAAACCTTAGTGAAGTGTTTGATTGTATCCGTTTTCGCCTTATGACAGAAAAATATTTTAAGG
  ATCATGTTGAGAAAGATGATATAATTAAAAGCAACCCAGACCTCCAGAAAAAAATCAAAGTTCTAAAAGA
  TGCTTTCGCAGGCAAACTCCCAGAACCTAGCAAAAATGCCGCGAAGACTGGGGCTGGTGAGGTGAATGGT
  GATGTTGGTGATGAAGATTTACTTCCTGGTTACCTGAATGACATTCCCAGGCATGGAATGTTTGTAAAAG
  ACCTCATCCTCTTGGTTAATGACACAGCAGCAGTGGCTTATGACCCCACGGAAAATGAATGCTACCTTAC
  TGCACTGGCTGAGCAGATTCCCAGAAATCATTCCAGCATTGTTACCCAGCAAAATCAGATATATGTGGTA
  GGAGGACTATATGTGGATGAAGAAAATAAGGATCAACCTCTACAGTCATACTTCTTCCAGCTCGATAGCA
  TAGCATCTGAATGGGTTGGACTTCCACCTCTGCCTTCAGCCAGGTGTCTCTTCGGTCTGGGAGAGGTGGA
  TGATAAAATCTATGTAGTTGCAGGCAAAGACCTTCAAACAGAGGCTTCGCTGGATTCAGTATTATGCTAT
  GATCCTGTGGCTGCAAAATGGAACGAAGTAAAAAAACTCCCTATCAAAGTCTATGGCCATAATGTGATTT
  CACATAAAGGGATGATATATTGTCTAGGAGGAAAGACAGATGACAAAAAATGTACAAACAGGGTGTTTAT
  CTTCAACCCCAAAAAAGGAGATTGGAAAGATCTGGCTCCAATGAAAATTCCTCGTTCCATGTTTGGAGTA
  GCAGTCCATAAAGGCAAAATTGTGATTGCAGGAGGTGTCACTGAAGATGGTCTTTCAGCTTCAGTTGAAG
  CTTTTGACCTTACAACAAATAAATGGGATGTAATGACCGAATTTCCCCAAGAAAGAAGCTCCATCAGTTT
  GGTCAGCCTGGCTGGATCTCTGTATGCAATTGGTGGTTTTGCTATGATTCAACTGGAGTCTAAAGAATTT
  GCACCCACTGAAGTCAATGACATATGGAAGTATGAAGATGATAAAAAAGAATGGGCTGGGATGTTGAAGG
  AAATACGTTATGCTTCAGGAGCTAGTTGCCTAGCAACACGTTTAAATCTCTTCAAACTGTCTAAACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200295 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDSQRELAEELRLYQSTLLQDGLKDLLDEKKFIDCTLKAGDKSLPCHRLILSACSPYFREYFLSEIDEAK
  KKEVVLDNVDPAILDLIIKYLYSASIDLNDGNVQDIFALASRFQIPSVFTVCVSYLQKRLAPGNCLAILR
  LGLLLDCPRLAISAREFVSDRFVQICKEEDFMQLSPQELISVISNDSLNVEKEEAVFEAVMKWVRTDKEN
  RVKNLSEVFDCIRFRLMTEKYFKDHVEKDDIIKSNPDLQKKIKVLKDAFAGKLPEPSKNAAKTGAGEVNG
  DVGDEDLLPGYLNDIPRHGMFVKDLILLVNDTAAVAYDPTENECYLTALAEQIPRNHSSIVTQQNQIYVV
  GGLYVDEENKDQPLQSYFFQLDSIASEWVGLPPLPSARCLFGLGEVDDKIYVVAGKDLQTEASLDSVLCY
  DPVAAKWNEVKKLPIKVYGHNVISHKGMIYCLGGKTDDKKCTNRVFIFNPKKGDWKDLAPMKIPRSMFGV
  AVHKGKIVIAGGVTEDGLSASVEAFDLTTNKWDVMTEFPQERSSISLVSLAGSLYAIGGFAMIQLESKEF
  APTEVNDIWKYEDDKKEWAGMLKEIRYASGASCLATRLNLFKLSKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006063
• ORF Size: 1818 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006063.3
• RefSeq Size: 2472 bp
• RefSeq ORF: 1821 bp
• Locus ID: 10324
• UniProt ID: O60662
• Cytogenetics: 2q31.1
• Domains: Kelch
• MW: 68 kDa
• Gene Summary: This gene is a member of the kelch-like family. The encoded protein contains a BACK domain, a BTB/POZ domain, and 5 Kelch repeats. This protein is thought to function in skeletal muscle development and maintenance. Mutations in this gene have been associated with nemaline myopathy (NM), a rare congenital muscle disorder. [provided by RefSeq, Mar 2015]