KBTBD10 (KLHL41) (NM_006063) Human Tagged ORF Clone

CAT#: RC200295

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KLHL41 (Myc-DDK-tagged)-Human kelch repeat and BTB (POZ) domain containing 10 (KBTBD10)



  "NM_006063" in other vectors (5)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 564.00

USD 846.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol KLHL41
Synonyms KBTBD10; Krp1; SARCOSIN
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200295 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATTCCCAGCGGGAACTTGCAGAGGAACTGCGGCTTTACCAATCCACCCTTCTTCAGGATGGTCTAA
AAGATCTCCTGGATGAGAAAAAATTCATCGATTGCACCCTAAAAGCAGGTGACAAAAGTCTTCCTTGCCA
CAGATTGATTTTGTCAGCTTGTAGTCCTTACTTCCGTGAGTACTTTTTATCTGAAATTGATGAGGCGAAA
AAAAAGGAGGTAGTGCTAGACAATGTGGATCCTGCTATACTTGATTTAATCATCAAATACCTGTACTCTG
CCAGTATTGATCTCAATGACGGAAATGTGCAAGATATTTTTGCATTGGCCAGCCGCTTTCAGATCCCCTC
AGTGTTTACTGTCTGCGTTTCTTATCTTCAGAAAAGACTTGCTCCTGGTAACTGTCTAGCCATCCTAAGA
TTAGGACTTCTTCTTGACTGCCCGAGACTCGCCATTTCTGCCCGTGAATTTGTGTCTGATCGCTTTGTAC
AGATTTGTAAGGAAGAGGACTTTATGCAACTGTCTCCACAGGAACTGATCTCAGTCATTTCAAATGACAG
CCTAAATGTAGAAAAAGAAGAAGCAGTATTTGAGGCAGTGATGAAATGGGTGCGAACAGACAAGGAAAAC
AGGGTTAAAAACCTTAGTGAAGTGTTTGATTGTATCCGTTTTCGCCTTATGACAGAAAAATATTTTAAGG
ATCATGTTGAGAAAGATGATATAATTAAAAGCAACCCAGACCTCCAGAAAAAAATCAAAGTTCTAAAAGA
TGCTTTCGCAGGCAAACTCCCAGAACCTAGCAAAAATGCCGCGAAGACTGGGGCTGGTGAGGTGAATGGT
GATGTTGGTGATGAAGATTTACTTCCTGGTTACCTGAATGACATTCCCAGGCATGGAATGTTTGTAAAAG
ACCTCATCCTCTTGGTTAATGACACAGCAGCAGTGGCTTATGACCCCACGGAAAATGAATGCTACCTTAC
TGCACTGGCTGAGCAGATTCCCAGAAATCATTCCAGCATTGTTACCCAGCAAAATCAGATATATGTGGTA
GGAGGACTATATGTGGATGAAGAAAATAAGGATCAACCTCTACAGTCATACTTCTTCCAGCTCGATAGCA
TAGCATCTGAATGGGTTGGACTTCCACCTCTGCCTTCAGCCAGGTGTCTCTTCGGTCTGGGAGAGGTGGA
TGATAAAATCTATGTAGTTGCAGGCAAAGACCTTCAAACAGAGGCTTCGCTGGATTCAGTATTATGCTAT
GATCCTGTGGCTGCAAAATGGAACGAAGTAAAAAAACTCCCTATCAAAGTCTATGGCCATAATGTGATTT
CACATAAAGGGATGATATATTGTCTAGGAGGAAAGACAGATGACAAAAAATGTACAAACAGGGTGTTTAT
CTTCAACCCCAAAAAAGGAGATTGGAAAGATCTGGCTCCAATGAAAATTCCTCGTTCCATGTTTGGAGTA
GCAGTCCATAAAGGCAAAATTGTGATTGCAGGAGGTGTCACTGAAGATGGTCTTTCAGCTTCAGTTGAAG
CTTTTGACCTTACAACAAATAAATGGGATGTAATGACCGAATTTCCCCAAGAAAGAAGCTCCATCAGTTT
GGTCAGCCTGGCTGGATCTCTGTATGCAATTGGTGGTTTTGCTATGATTCAACTGGAGTCTAAAGAATTT
GCACCCACTGAAGTCAATGACATATGGAAGTATGAAGATGATAAAAAAGAATGGGCTGGGATGTTGAAGG
AAATACGTTATGCTTCAGGAGCTAGTTGCCTAGCAACACGTTTAAATCTCTTCAAACTGTCTAAACTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200295 protein sequence
Red=Cloning site Green=Tags(s)

MDSQRELAEELRLYQSTLLQDGLKDLLDEKKFIDCTLKAGDKSLPCHRLILSACSPYFREYFLSEIDEAK
KKEVVLDNVDPAILDLIIKYLYSASIDLNDGNVQDIFALASRFQIPSVFTVCVSYLQKRLAPGNCLAILR
LGLLLDCPRLAISAREFVSDRFVQICKEEDFMQLSPQELISVISNDSLNVEKEEAVFEAVMKWVRTDKEN
RVKNLSEVFDCIRFRLMTEKYFKDHVEKDDIIKSNPDLQKKIKVLKDAFAGKLPEPSKNAAKTGAGEVNG
DVGDEDLLPGYLNDIPRHGMFVKDLILLVNDTAAVAYDPTENECYLTALAEQIPRNHSSIVTQQNQIYVV
GGLYVDEENKDQPLQSYFFQLDSIASEWVGLPPLPSARCLFGLGEVDDKIYVVAGKDLQTEASLDSVLCY
DPVAAKWNEVKKLPIKVYGHNVISHKGMIYCLGGKTDDKKCTNRVFIFNPKKGDWKDLAPMKIPRSMFGV
AVHKGKIVIAGGVTEDGLSASVEAFDLTTNKWDVMTEFPQERSSISLVSLAGSLYAIGGFAMIQLESKEF
APTEVNDIWKYEDDKKEWAGMLKEIRYASGASCLATRLNLFKLSKL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_006063
ORF Size 1818 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006063.3
RefSeq Size 2472 bp
RefSeq ORF 1821 bp
Locus ID 10324
UniProt ID O60662
Cytogenetics 2q31.1
Domains Kelch
MW 68 kDa
Gene Summary This gene is a member of the kelch-like family. The encoded protein contains a BACK domain, a BTB/POZ domain, and 5 Kelch repeats. This protein is thought to function in skeletal muscle development and maintenance. Mutations in this gene have been associated with nemaline myopathy (NM), a rare congenital muscle disorder. [provided by RefSeq, Mar 2015]

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