PACSIN2 (NM_007229) Human Tagged ORF Clone

CAT#: RC200237

PACSIN2 (Myc-DDK-tagged)-Human protein kinase C and casein kinase substrate in neurons 2 (PACSIN2), transcript variant 2

Product Images

Circular map for RC200237

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  Western blot validation of overexpression lysate (Cat# LY416115) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200237 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PACSIN2 protein (Cat# TP300237). The protein was produced from HEK293T cells transfected with PACSIN2 cDNA clone (Cat# RC200237) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PACSIN2
• Synonyms: SDPII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200237 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGTCACATATGATGATTCCGTTGGAGTAGAAGTGTCCAGCGACAGCTTCTGGGAGGTCGGGAACT
  ACAAGCGGACTGTGAAGCGGATCGACGATGGCCACCGCCTGTGCAGCGACCTCATGAACTGCCTGCATGA
  GCGGGCGCGCATCGAGAAGGCGTATGCGCAGCAGCTCACTGAGTGGGCCCGGCGCTGGAGGCAGCTCGTG
  GAGAAAGGGCCCCAGTACGGGACCGTGGAGAAGGCCTGGATGGCCTTCATGTCCGAGGCAGAGAGGGTGA
  GCGAGCTGCACCTCGAGGTGAAGGCCTCACTGATGAACGATGACTTCGAGAAGATCAAGAACTGGCAGAA
  GGAAGCCTTTCACAAGCAGATGATGGGCGGCTTCAAGGAGACCAAGGAAGCTGAGGACGGCTTTCGGAAG
  GCACAGAAGCCCTGGGCCAAGAAGCTGAAAGAGGTAGAAGCAGCAAAGAAAGCCCACCATGCAGCGTGCA
  AAGAGGAGAAGCTGGCTATCTCACGAGAAGCCAACAGCAAGGCAGACCCATCCCTCAACCCTGAACAGCT
  CAAGAAATTGCAAGACAAAATAGAAAAGTGCAAGCAAGATGTTCTTAAGACCAAAGAGAAGTATGAGAAG
  TCCCTGAAGGAACTCGACCAGGGCACACCCCAGTACATGGAGAACATGGAGCAGGTGTTTGAGCAGTGCC
  AGCAGTTCGAGGAGAAACGCCTTCGCTTCTTCCGGGAGGTTCTGCTGGAGGTTCAGAAGCACCTAGACCT
  GTCCAATGTGGCTGGCTACAAAGCCATTTACCATGACCTGGAGCAGAGCATCAGAGCAGCTGATGCAGTG
  GAGGACCTGAGGTGGTTCCGAGCCAATCACGGGCCGGGCATGGCCATGAACTGGCCGCAGTTTGAGGAGT
  GGTCCGCAGACCTGAATCGAACCCTCAGCCGGAGAGAGAAGAAGAAGGCCACTGACGGCGTCACCCTGAC
  GGGCATCAACCAGACAGGCGACCAGTCTCTGCCGAGTAAGCCCAGCAGCACCCTTAATGTCCCGAGCAAC
  CCCGCCCAGTCTGCGCAGTCACAGTCCAGCTACAACCCCTTCGAGGATGAGGACGACACGGGCAGCACCG
  TCAGTGAGAAGGACGACACTAAGGCCAAAAATGTGAGCAGCTACGAGAAGACCCAGAGCTATCCCACCGA
  CTGGTCAGACGATGAGTCTAACAACCCCTTCTCCTCCACGGATGCCAATGGGGACTCGAATCCATTCGAC
  GACGACGCCACCTCGGGGACGGAAGTGCGAGTCCGGGCCCTGTATGACTATGAGGGGCAGGAGCATGATG
  AGCTGAGCTTCAAGGCTGGGGATGAGCTGACCAAGATGGAGGACGAGGATGAGCAGGGCTGGTGCAAGGG
  ACGCTTGGACAACGGGCAAGTTGGCCTATACCCGGCAAATTATGTGGAGGCGATCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200237 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSVTYDDSVGVEVSSDSFWEVGNYKRTVKRIDDGHRLCSDLMNCLHERARIEKAYAQQLTEWARRWRQLV
  EKGPQYGTVEKAWMAFMSEAERVSELHLEVKASLMNDDFEKIKNWQKEAFHKQMMGGFKETKEAEDGFRK
  AQKPWAKKLKEVEAAKKAHHAACKEEKLAISREANSKADPSLNPEQLKKLQDKIEKCKQDVLKTKEKYEK
  SLKELDQGTPQYMENMEQVFEQCQQFEEKRLRFFREVLLEVQKHLDLSNVAGYKAIYHDLEQSIRAADAV
  EDLRWFRANHGPGMAMNWPQFEEWSADLNRTLSRREKKKATDGVTLTGINQTGDQSLPSKPSSTLNVPSN
  PAQSAQSQSSYNPFEDEDDTGSTVSEKDDTKAKNVSSYEKTQSYPTDWSDDESNNPFSSTDANGDSNPFD
  DDATSGTEVRVRALYDYEGQEHDELSFKAGDELTKMEDEDEQGWCKGRLDNGQVGLYPANYVEAIQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007229
• ORF Size: 1458 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007229.3
• RefSeq Size: 3292 bp
• RefSeq ORF: 1461 bp
• Locus ID: 11252
• UniProt ID: Q9UNF0
• Cytogenetics: 22q13.2
• Domains: FCH, SH3
• MW: 55.7 kDa
• Gene Summary: This gene is a member of the protein kinase C and casein kinase substrate in neurons family. The encoded protein is involved in linking the actin cytoskeleton with vesicle formation by regulating tubulin polymerization. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]