AKAP8L (NM_014371) Human Tagged ORF Clone

CAT#: RC200233

AKAP8L (Myc-DDK-tagged)-Human A kinase (PRKA) anchor protein 8-like (AKAP8L)

Product Images

Circular map for RC200233

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  Western blot validation of overexpression lysate (Cat# LY415335) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200233 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified AKAP8L protein (Cat# TP300233). The protein was produced from HEK293T cells transfected with AKAP8L cDNA clone (Cat# RC200233) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AKAP8L
• Synonyms: HA95; HAP95; NAKAP; NAKAP95
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200233 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTACACAGGCTTTGTCCAGGGATCTGAAACCACTTTGCAGTCGACATACTCGGATACCAGCGCTC
  AGCCCACCTGTGATTATGGATATGGAACTTGGAACTCTGGGACAAATAGAGGCTACGAGGGCTATGGCTA
  TGGCTATGGCTATGGCCAGGATAACACCACCAACTATGGGTATGGTATGGCCACTTCACACTCTTGGGAA
  ATGCCTAGCTCTGACACAAATGCAAACACTAGTGCCTCGGGTAGCGCCAGTGCCGATTCCGTTTTATCCA
  GAATTAACCAGCGCTTAGATATGGTGCCGCATTTGGAGACAGACATGATGCAAGGAGGCGTGTACGGCTC
  AGGTGGAGAAAGGTATGACTCTTATGAGTCCTGCGACTCGAGGGCCGTCCTGAGTGAGCGCGACCTGTAC
  CGGTCAGGCTATGACTACAGCGAGCTTGACCCTGAGATGGAAATGGCCTATGAGGGCCAATACGATGCCT
  ACCGCGACCAGTTCCGCATGCGTGGCAACGACACCTTCGGTCCCAGGGCACAGGGCTGGGCCCGGGATGC
  CCGGAGCGGCCGGCCAATGGCCTCAGGCTATGGGCGCATGTGGGAAGACCCCATGGGGGCCCGGGGCCAG
  TGCATGTCTGGTGCCTCTCGGCTGCCCTCCCTCTTCTCCCAGAACATCATCCCCGAGTACGGCATGTTCC
  AGGGCATGCGAGGTGGGGGCGCCTTCCCGGGCGGCTCCCGCTTTGGTTTCGGGTTTGGCAATGGCATGAA
  GCAGATGAGGCGGACCTGGAAGACCTGGACCACAGCCGACTTCCGAACCAAGAAGAAGAAGAGAAAGCAG
  GGCGGCAGTCCTGATGAGCCAGATAGCAAAGCCACCCGCACGGACTGCTCGGACAACAGCGACTCAGACA
  ATGATGAGGGCACCGAGGGGGAAGCCACAGAGGGCCTTGAAGGCACCGAGGCTGTGGAGAAGGGCTCCAG
  AGTGGACGGAGAGGATGAGGAGGGAAAAGAGGATGGGAGAGAAGAAGGCAAAGAGGATCCAGAGAAGGGG
  GCCCTAACCACCCAGGATGAAAATGGCCAGACCAAGCGCAAGTTGCAGGCAGGCAAGAAGAGTCAGGACA
  AGCAGAAAAAGCGGCAGCGAGACCGCATGGTGGAAAGGATCCAGTTTGTGTGTTCTCTGTGCAAATACCG
  GACCTTCTATGAGGACGAGATGGCCAGCCATCTTGACAGCAAGTTCCACAAGGAACACTTTAAGTACGTA
  GGCACCAAGCTCCCTAAGCAGACGGCTGACTTTCTGCAGGAGTACGTCACTAACAAGACCAAGAAGACAG
  AGGAGCTCCGAAAAACCGTGGAGGACCTTGATGGCCTCATCCAGCAAATCTACAGAGACCAGGATCTGAC
  CCAGGAAATTGCCATGGAGCATTTTGTGAAGAAGGTGGAGGCAGCCCATTGTGCAGCCTGCGACCTCTTC
  ATTCCCATGCAGTTTGGGATCATCCAGAAGCATCTGAAGACCATGGATCACAACCGGAACCGCAGGCTCA
  TGATGGAGCAGTCCAAGAAGTCCTCCCTCATGGTGGCCCGCAGTATTCTCAACAACAAGCTCATCAGCAA
  GAAGCTGGAGCGCTACCTGAAGGGCGAGAACCCTTTCACCGACAGCCCCGAGGAGGAGAAGGAGCAGGAG
  GAGGCTGAGGGCGGTGCCCTGGACGAGGGGGCGCAGGGCGAAGCGGCAGGGATCTCGGAGGGCGCAGAGG
  GCGTGCCGGCGCAGCCTCCCGTGCCCCCAGAGCCAGCCCCCGGGGCCGTGTCGCCGCCACCGCCGCCGCC
  CCCAGAGGAGGAGGAGGAGGGCGCCGTGCCCTTGCTGGGAGGGGCGCTGCAACGCCAGATCCGCGGCATC
  CCGGGCCTCGACGTGGAGGACGACGAGGAGGGCGGCGGGGGCGCCCCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200233 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSYTGFVQGSETTLQSTYSDTSAQPTCDYGYGTWNSGTNRGYEGYGYGYGYGQDNTTNYGYGMATSHSWE
  MPSSDTNANTSASGSASADSVLSRINQRLDMVPHLETDMMQGGVYGSGGERYDSYESCDSRAVLSERDLY
  RSGYDYSELDPEMEMAYEGQYDAYRDQFRMRGNDTFGPRAQGWARDARSGRPMASGYGRMWEDPMGARGQ
  CMSGASRLPSLFSQNIIPEYGMFQGMRGGGAFPGGSRFGFGFGNGMKQMRRTWKTWTTADFRTKKKKRKQ
  GGSPDEPDSKATRTDCSDNSDSDNDEGTEGEATEGLEGTEAVEKGSRVDGEDEEGKEDGREEGKEDPEKG
  ALTTQDENGQTKRKLQAGKKSQDKQKKRQRDRMVERIQFVCSLCKYRTFYEDEMASHLDSKFHKEHFKYV
  GTKLPKQTADFLQEYVTNKTKKTEELRKTVEDLDGLIQQIYRDQDLTQEIAMEHFVKKVEAAHCAACDLF
  IPMQFGIIQKHLKTMDHNRNRRLMMEQSKKSSLMVARSILNNKLISKKLERYLKGENPFTDSPEEEKEQE
  EAEGGALDEGAQGEAAGISEGAEGVPAQPPVPPEPAPGAVSPPPPPPPEEEEEGAVPLLGGALQRQIRGI
  PGLDVEDDEEGGGGAP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014371
• ORF Size: 1938 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014371.4
• RefSeq Size: 2231 bp
• RefSeq ORF: 1941 bp
• Locus ID: 26993
• UniProt ID: Q9ULX6
• Cytogenetics: 19p13.12
• Domains: AKAP95
• Protein Families: Druggable Genome
• MW: 71.6 kDa
• Gene Summary: Could play a role in constitutive transport element (CTE)-mediated gene expression by association with DHX9. Increases CTE-dependent nuclear unspliced mRNA export (PubMed:10748171, PubMed:11402034). Proposed to target PRKACA to the nucleus but does not seem to be implicated in the binding of regulatory subunit II of PKA (PubMed:10761695, PubMed:11884601). May be involved in nuclear envelope breakdown and chromatin condensation. May be involved in anchoring nuclear membranes to chromatin in interphase and in releasing membranes from chromating at mitosis (PubMed:11034899). May regulate the initiation phase of DNA replication when associated with TMPO isoform Beta (PubMed:12538639). Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3; in this function seems to act redundantly with AKAP8 (PubMed:16980585). May be involved in regulation of pre-mRNA splicing (PubMed:17594903).[UniProtKB/Swiss-Prot Function]