GNL2 (NM_013285) Human Tagged ORF Clone

CAT#: RC200184

GNL2 (Myc-DDK-tagged)-Human guanine nucleotide binding protein-like 2 (nucleolar) (GNL2)

Product Images

Circular map for RC200184

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GNL2
• Synonyms: HUMAUANTIG; Ngp-1; NGP1; Nog2; Nug2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200184 representing NM_013285
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAAGCCCAAGTACAAAGGACGGAGCACCATCAACCCGTCCAAGGCCAGCACAAACCCAGATCGAG
  TGCAGGGAGCAGGAGGCCAAAACATGAGGGACCGGGCCACCATCCGGCGCCTGAATATGTATAGGCAAAA
  GGAGCGCAGGAACAGTCGTGGTAAAATAATTAAACCCCTGCAATATCAATCAACGGTGGCTTCTGGCACA
  GTGGCAAGAGTAGAGCCAAATATTAAATGGTTTGGAAACACACGTGTGATTAAGCAGTCATCATTACAAA
  AATTTCAAGAGGAAATGGATACAGTTATGAAGGATCCATACAAAGTTGTCATGAAGCAAAGCAAGTTACC
  AATGTCTCTTCTCCATGATCGAATCCGGCCTCATAACTTGAAGGTGCACATTCTTGATACTGAAAGTTTT
  GAAACTACATTTGGCCCTAAGTCACAGAGGAAACGACCAAACTTATTTGCAAGTGATATGCAGTCTCTTA
  TCGAAAATGCTGAAATGTCCACTGAGAGCTATGACCAGGGCAAGGATCGTGATTTGGTAACTGAAGACAC
  TGGTGTGAGAAATGAAGCTCAAGAAGAGATCTATAAAAAGGGACAGTCCAAAAGAATATGGGGTGAGCTC
  TACAAGGTGATAGATTCATCAGATGTTGTAGTTCAAGTTCTTGATGCTAGAGATCCAATGGGTACTCGTT
  CCCCTCACATTGAAACTTACCTGAAGAAGGAAAAACCTTGGAAACACCTCATTTTTGTACTTAACAAATG
  TGACCTTGTTCCAACCTGGGCAACAAAACGGTGGGTTGCTGTCCTCTCCCAGGATTATCCAACACTTGCT
  TTCCATGCAAGCCTTACTAACCCGTTTGGCAAGGGAGCATTCATTCAGCTTCTGCGGCAGTTTGGAAAGT
  TGCACACTGACAAGAAACAGATCAGTGTTGGGTTCATTGGCTATCCAAATGTTGGCAAGAGCTCTGTGAT
  AAATACATTGCGTTCTAAGAAAGTTTGCAACGTGGCTCCCATTGCAGGTGAAACAAAGGTCTGGCAGTAT
  ATTACTTTGATGCGTCGGATATTCCTGATTGACTGTCCAGGTGTGGTTTACCCCTCTGAGGACTCCGAGA
  CAGACATTGTGCTAAAAGGAGTTGTTCAAGTAGAAAAAATTAAGAGTCCTGAAGACCACATTGGTGCTGT
  ACTTGAACGAGCAAAGCCAGAATATATCAGCAAAACATACAAGATTGATTCTTGGGAGAATGCTGAGGAC
  TTTCTTGAGAAGCTCGCTTTCCGGACTGGGAAGTTACTAAAGGGTGGAGAGCCCGACTTGCAGACTGTGG
  GTAAGATGGTCCTCAATGACTGGCAGAGGGGCCGGATTCCTTTCTTTGTCAAGCCACCCAATGCAGAGCC
  ACTTGTGGCCCCCCAGCTTCTACCCTCCTCATCTTTGGAAGTTGTCCCAGAAGCAGCCCAGAACAATCCA
  GGGGAGGAAGTCACAGAAACTGCAGGTGAAGGGTCAGAATCCATCATTAAGGAAGAAACAGAAGAGAACA
  GTCACTGTGATGCTAACACAGAGATGCAGCAGATTCTCACACGAGTTCGGCAGAACTTTGGTAAAATCAA
  CGTGGTGCCTCAGTTTTCTGGGGATGACCTGGTTCCTGTGGAGGTGTCAGATCTTGAGGAAGAGCTTGAG
  AGCTTTTCTGATGAAGAGGAGGAGGAACAGGAGCAACAAAGAGATGATGCGGAAGAGTCTTCCTCGGAGC
  CTGAGGAGGAAAATGTGGGAAACGACACCAAAGCCGTTATTAAAGCACTGGATGAGAAGATTGCCAAATA
  TCAGAAGTTTCTAGACAAAGCCAAAGCCAAAAAGTTTTCAGCAGTCAGAATATCCAAGGGACTGAGTGAA
  AAGATATTTGCAAAACCTGAAGAACAAAGAAAAACACTGGAAGAAGATGTAGATGACAGAGCACCTTCCA
  AAAAGGGAAAGAAGCGGAAGGCACAAAGGGAAGAGGAACAGGAACATTCAAATAAAGCTCCCAGGGCGCT
  TACATCAAAAGAACGGAGGCGAGCAGTACGACAGCAACGGCCGAAAAAAGTTGGTGTGCGCTACTATGAA
  ACACACAACGTGAAAAATAGGAACAGGAACAAAAAGAAGACCAATGACTCAGAGGGACAGAAACACAAAC
  GCAAAAAATTCAGACAAAAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200184 representing NM_013285
  Red=Cloning site Green=Tags(s)
  
  MVKPKYKGRSTINPSKASTNPDRVQGAGGQNMRDRATIRRLNMYRQKERRNSRGKIIKPLQYQSTVASGT
  VARVEPNIKWFGNTRVIKQSSLQKFQEEMDTVMKDPYKVVMKQSKLPMSLLHDRIRPHNLKVHILDTESF
  ETTFGPKSQRKRPNLFASDMQSLIENAEMSTESYDQGKDRDLVTEDTGVRNEAQEEIYKKGQSKRIWGEL
  YKVIDSSDVVVQVLDARDPMGTRSPHIETYLKKEKPWKHLIFVLNKCDLVPTWATKRWVAVLSQDYPTLA
  FHASLTNPFGKGAFIQLLRQFGKLHTDKKQISVGFIGYPNVGKSSVINTLRSKKVCNVAPIAGETKVWQY
  ITLMRRIFLIDCPGVVYPSEDSETDIVLKGVVQVEKIKSPEDHIGAVLERAKPEYISKTYKIDSWENAED
  FLEKLAFRTGKLLKGGEPDLQTVGKMVLNDWQRGRIPFFVKPPNAEPLVAPQLLPSSSLEVVPEAAQNNP
  GEEVTETAGEGSESIIKEETEENSHCDANTEMQQILTRVRQNFGKINVVPQFSGDDLVPVEVSDLEEELE
  SFSDEEEEEQEQQRDDAEESSSEPEEENVGNDTKAVIKALDEKIAKYQKFLDKAKAKKFSAVRISKGLSE
  KIFAKPEEQRKTLEEDVDDRAPSKKGKKRKAQREEEQEHSNKAPRALTSKERRRAVRQQRPKKVGVRYYE
  THNVKNRNRNKKKTNDSEGQKHKRKKFRQKQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013285
• ORF Size: 2193 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013285.3
• RefSeq Size: 2419 bp
• RefSeq ORF: 2196 bp
• Locus ID: 29889
• UniProt ID: Q13823
• Cytogenetics: 1p34.3
• Domains: MMR_HSR1
• MW: 83.7 kDa
• Gene Summary: GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation (By similarity). May promote cell proliferation possibly by increasing p53/TP53 protein levels, and consequently those of its downstream product CDKN1A/p21, and decreasing RPL23A protein levels (PubMed:26203195).[UniProtKB/Swiss-Prot Function]