HAUS4 (NM_017815) Human Tagged ORF Clone

CAT#: RC200173

HAUS4 (Myc-DDK-tagged)-Human HAUS augmin-like complex, subunit 4 (HAUS4), transcript variant 2

Product Images

Circular map for RC200173

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  Western blot validation of overexpression lysate (Cat# LY413524) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200173 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified HAUS4 protein (Cat# TP300173). The protein was produced from HEK293T cells transfected with HAUS4 cDNA clone (Cat# RC200173) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HAUS4
• Synonyms: C14orf94
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200173 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATCCGGGGATTTCTGCTCACCTGGAGAAGGGATGGAAATACTTCAACAAGTGTGCAGCAAACAAC
  TTCCTCCTTGTAACCTGAGTAAAGAGGACCTGTTACAGAACCCATACTTCAGCAAGCTTCTCCTGAATCT
  CTCACAGCATGTGGATGAGAGTGGCTTAAGCCTCACCCTAGCAAAGGAGCAGGCTCAGGCATGGAAGGAA
  GTTCGACTGCATAAGACAACATGGTTGAGGTCTGAGATTTTACACAGAGTCATTCAAGAGTTGCTTGTGG
  ACTACTATGTGAAGATACAAGACACAAATGTAACTTCTGAGGACAAAAAGTTTCATGAGACCCTTGAACA
  GCGGCTGCTTGTAACTGAACTGATGCGGCTCTTAGGTCCTAGCCAGGAGAGGGAGATACCTCCACTGCTG
  GGGCTGGAGAAAGCGGACCTTCTGGAACTCATGCCACTCTCAGAGGATTTTGTGTGGATGAGGGCTCGGC
  TACAGCAAGAAGTAGAGGAGCAGCTCAAAAAGAAATGTTTCACTCTGCTCTGCTACTATGATCCCAATTC
  AGATGCTGACAGTGAAACCGTGAAGGCAGCAAAGGTGTGGAAACTCGCAGAGGTCCTGGTGGGTGAGCAG
  CAGCAGTGCCAGGATGCCAAGAGCCAGCAGAAGGAGCAGATGTTGCTGCTGGAGAAGAAGAGTGCTGCTT
  ACTCCCAGGTGCTTCTCCGCTGCCTCACTTTGCTGCAGAGGCTTCTTCAAGAACACCGGCTGAAGACTCA
  ATCCGAGCTAGACCGCATCAATGCCCAGTACCTGGAAGTCAAGTGCGGTGCTATGATCCTTAAGCTGAGG
  ATGGAGGAGCTAAAGATTTTGTCCGACACTTACACTGTTGAGAAAGTGGAAGTTCATCGTCTGATTAGGG
  ACCGTTTGGAGGGAGCCATTCACCTACAGGAGCAGGACATGGAGAACTCAAGACAGGTCCTGAACTCCTA
  TGAGGTCCTTGGGGAGGAGTTTGACAGGCTGGTGAAAGAGTACACCGTACTCAAGCAGGCAACAGAGAAC
  AAGCGGTGGGCCCTCCAGGAGTTCAGCAAGGTCTACCGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200173 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASGDFCSPGEGMEILQQVCSKQLPPCNLSKEDLLQNPYFSKLLLNLSQHVDESGLSLTLAKEQAQAWKE
  VRLHKTTWLRSEILHRVIQELLVDYYVKIQDTNVTSEDKKFHETLEQRLLVTELMRLLGPSQEREIPPLL
  GLEKADLLELMPLSEDFVWMRARLQQEVEEQLKKKCFTLLCYYDPNSDADSETVKAAKVWKLAEVLVGEQ
  QQCQDAKSQQKEQMLLLEKKSAAYSQVLLRCLTLLQRLLQEHRLKTQSELDRINAQYLEVKCGAMILKLR
  MEELKILSDTYTVEKVEVHRLIRDRLEGAIHLQEQDMENSRQVLNSYEVLGEEFDRLVKEYTVLKQATEN
  KRWALQEFSKVYR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017815
• ORF Size: 1089 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017815.3
• RefSeq Size: 1649 bp
• RefSeq ORF: 1092 bp
• Locus ID: 54930
• UniProt ID: Q9H6D7
• Cytogenetics: 14q11.2
• MW: 42.4 kDa
• Gene Summary: This gene encodes a subunit of the centrosome complex termed the human augmin complex. The encoded protein localizes to the spindle microtubules and may play a role in mitotic spindle assembly and maintenance of centrosome integrity during cell division. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 1. [provided by RefSeq, Oct 2009]