NSUN5 (NM_148956) Human Tagged ORF Clone

CAT#: RC200144

NSUN5 (Myc-DDK-tagged)-Human NOP2/Sun domain family, member 5 (NSUN5), transcript variant 1

Product Images

Circular map for RC200144

• 

  Western blot validation of overexpression lysate (Cat# LY403452) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200144 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NSUN5
• Synonyms: NOL1; NOL1R; NSUN5A; p120; p120(NOL1); WBSCR20; WBSCR20A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200144 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCTGTATGCTGCAGCTGCAGGCGTGTTGGCCGGCGTGGAGAGCCGCCAGGGCTCTATCAAGGGGT
  TGGTGTACTCCAGCAACTTCCAGAACGTGAAGCAGCTGTACGCGCTGGTGTGCGAAACGCAGCGCTACTC
  CGCCGTGCTGGATGCTGTGATCGCCAGCGCCGGCCTCCTCCGTGCGGAGAAGAAGCTGCGGCCGCACCTG
  GCCAAGGTGCTAGTGTATGAGTTGTTGTTGGGAAAGGGCTTTCGAGGGGGTGGGGGCCGATGGAAGGCTC
  TGTTGGGCCGGCACCAGGCGAGGCTCAAGGCTGAGTTGGCTCGGCTCAAGGTTCATCGGGGTGTGAGCCG
  GAATGAGGACCTGTTGGAAGTGGGATCCAGGCCTGGTCCAGCCTCCCAGCTGCCTCGATTTGTGCGTGTG
  AACACTCTCAAGACCTGCTCCGATGATGTAGTTGATTATTTCAAGAGACAAGGTTTCTCCTATCAGGGTC
  GGGCTTCCAGCCTCGATGACTTACGAGCCCTCAAGGGGAAGCATTTTCTCCTGGACCCCTTGATGCCGGA
  GCTGCTGGTGTTTCCCGCCCAGACAGATCTGCATGAACACCCACTGTACCGGGCCGGACACCTCATTCTG
  CAGGACAGGGCCAGCTGTCTCCCAGCCATGCTGCTGGACCCCCCGCCAGGCTCCCATGTCATCGATGCCT
  GTGCCGCCCCAGGCAATAAGACCAGTCACTTGGCTGCTCTTCTGAAGAACCAAGGGAAGATCTTTGCCTT
  TGACCTGGATGCCAAGCGGCTGGCATCCATGGCCACGCTGCTGGCCCGGGCTGGCGTCTCTTGCTGTGAA
  CTGGCTGAGGAGGACTTCCTGGCGGTCTCCCCCTCGGATCCACGCTACCATGAGGTCCACTACATCCTGC
  TGGATCCTTCCTGCAGTGGCTCGGGTATGCCGAGCAGACAGCTGGAGGAGCCCGGGGCAGGCACACCTAG
  CCCGGTGCGTCTGCATGCCCTGGCAGGGTTCCAGCAGCGAGCCCTGTGCCACGCACTCACTTTCCCTTCC
  CTGCAGCGGCTCGTCTACTCCACGTGCTCCCTCTGCCAGGAGGAGAATGAAGACGTGGTGCGAGATGCGC
  TGCAGCAGAACCCGGGCGCCTTCAGGCTAGCTCCCGCCCTGCCTGCCTGGCCCCACCGAGGCCTGAGCAC
  GTTCCCGGGTGCCGAGCACTGCCTCCGGGCCTCCCCTGAGACCACACTCAGCAGTGGCTTCTTCGTTGCT
  GTAATTGAACGGGTCGAGGTGCCAAGCTCAGCCTCACAGGCCAAAGCATCAGCACCAGAACGCACACCCA
  GCCCAGCCCCAAAGAGAAAGAAGAGACAGCAAAGAGCCGCAGCCGGTGCTTGCACACCGCCTTGCACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200144 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGLYAAAAGVLAGVESRQGSIKGLVYSSNFQNVKQLYALVCETQRYSAVLDAVIASAGLLRAEKKLRPHL
  AKVLVYELLLGKGFRGGGGRWKALLGRHQARLKAELARLKVHRGVSRNEDLLEVGSRPGPASQLPRFVRV
  NTLKTCSDDVVDYFKRQGFSYQGRASSLDDLRALKGKHFLLDPLMPELLVFPAQTDLHEHPLYRAGHLIL
  QDRASCLPAMLLDPPPGSHVIDACAAPGNKTSHLAALLKNQGKIFAFDLDAKRLASMATLLARAGVSCCE
  LAEEDFLAVSPSDPRYHEVHYILLDPSCSGSGMPSRQLEEPGAGTPSPVRLHALAGFQQRALCHALTFPS
  LQRLVYSTCSLCQEENEDVVRDALQQNPGAFRLAPALPAWPHRGLSTFPGAEHCLRASPETTLSSGFFVA
  VIERVEVPSSASQAKASAPERTPSPAPKRKKRQQRAAAGACTPPCT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_148956
• ORF Size: 1398 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_148956.4
• RefSeq Size: 2377 bp
• RefSeq ORF: 1401 bp
• Locus ID: 55695
• UniProt ID: Q96P11
• Cytogenetics: 7q11.23
• Domains: Nol1_Nop2_Sun
• MW: 50.4 kDa
• Gene Summary: This gene encodes a member of an evolutionarily conserved family of proteins that may function as methyltransferases. This gene is located in a larger region of chromosome 7 that is deleted in Williams-Beuren syndrome, a multisystem developmental disorder. There are two pseudogenes for this gene located in the same region of chromosome 7. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]