ENTR1 (NM_006643) Human Tagged ORF Clone

CAT#: RC200139

SDCCAG3 (Myc-DDK-tagged)-Human serologically defined colon cancer antigen 3 (SDCCAG3), transcript variant 2

Product Images

Circular map for RC200139

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  Western blot validation of overexpression lysate (Cat# LY402000) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200139 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ENTR1 protein (Cat# TP300139). The protein was produced from HEK293T cells transfected with ENTR1 cDNA clone (Cat# RC200139) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ENTR1
• Synonyms: NY-CO-3; SDCCAG3; SDDAG3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200139 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGGCTACCAGCGCCACCCGGGCGCCACCCCGCTGTCCCGAGCCCGGAGCCTCGCCATTCCCGACG
  CTCCAGCGTTCTATGAGCGCCGGTCTTGTCTCCCCCAGCTAAATTGTGAGCGCCCCCATGGCAGGGACCT
  GGACTCCCCCTTCTTCGGCATTCGGCCGGCCTTTATGTGCTATGTGCCCAGCCCGGTGCTGGCTTCCGTG
  GGAGACACAGATGACAGATTTGAAGATCTGGAAGAGGCAAATCCATTCTCTTTTAGAGAGTTTCTGAAGA
  CCAAGAACCTCGGCCTCTCGAAAGAGGATCCGGCCAGCAGAATTTATGCAAAGGAAGCCTCGAGGCATTC
  CCTGGGACTTGACCACAACTCCCCACCCTCCCAAACCGGCGGGTATGGCCTGGAGTATCAGCAGCCATTT
  TTCGAGGATCCGACAGGGGCTGGTGACCTCCTGGATGGGGAGGAGGATGAGGACACCGGATGGAGTGGGG
  CCTACCTGCCGTCCGCCATCGAGCAGACTCACCCCGAGAGGGTCCCTGCCGGCACGTCGCCCTGCAGCAC
  ATACCTTTCCTTTTTCTCCACCCCGTCGGAGCTGGCAGGGCCTGAGTCTCTGCCCTCGTGGGCGTTGAGT
  GACACTGATTCTCGCGTGTCTCCGGCCTCTCCGGCAGGGAGTCCTAGCGCAGACTTTGCGGTTCATGGAG
  AGTCTCTGGGAGACAGGCACCTGCGGACGCTGCAGATAAGTTACGACGCACTGAAAGATGAAAATTCTAA
  GCTGAGAAGAAAGCTGAATGAGGTTCAGAGCTTCTCTGAAGCTCAAACAGAAATGGTGAGGACGCTTGAG
  CGGAAGTTAGAAGCAAAAATGATCAAGGAGGAAAGCGACTACCACGACCTGGAGTCGGTGGTTCAGCAGG
  TGGAGCAGAACCTGGAGCTGATGACCAAACGGGCTGTAAAGGCAGAAAACCACGTCGTGAAACTAAAACA
  GGAAATCAGTTTGCTCCAGGCGCAGGTCTCCAACTTCCAGCGAGAGAATGAAGCCCTGCGGTGCGGCCAG
  GGCGCCAGCCTGACCGTGGTGAAGCAGAACGCCGACGTGGCCCTGCAGAACCTCCGGGTGGTCATGAACA
  GTGCACAGGCTTCCATCAAGCAACTGGTTTCCGGAGCTGAGACACTGAATCTTGTTGCCGAAATCCTTAA
  ATCTATAGACAGAATTTCTGAAATTAAAGACGAGGAGGAAGACTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200139 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSGYQRHPGATPLSRARSLAIPDAPAFYERRSCLPQLNCERPHGRDLDSPFFGIRPAFMCYVPSPVLASV
  GDTDDRFEDLEEANPFSFREFLKTKNLGLSKEDPASRIYAKEASRHSLGLDHNSPPSQTGGYGLEYQQPF
  FEDPTGAGDLLDGEEDEDTGWSGAYLPSAIEQTHPERVPAGTSPCSTYLSFFSTPSELAGPESLPSWALS
  DTDSRVSPASPAGSPSADFAVHGESLGDRHLRTLQISYDALKDENSKLRRKLNEVQSFSEAQTEMVRTLE
  RKLEAKMIKEESDYHDLESVVQQVEQNLELMTKRAVKAENHVVKLKQEISLLQAQVSNFQRENEALRCGQ
  GASLTVVKQNADVALQNLRVVMNSAQASIKQLVSGAETLNLVAEILKSIDRISEIKDEEEDS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006643
• ORF Size: 1236 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006643.3, NP_006634.3
• RefSeq Size: 2321 bp
• RefSeq ORF: 1239 bp
• Locus ID: 10807
• UniProt ID: Q96C92
• Cytogenetics: 9q34.3
• MW: 45.5 kDa
• Gene Summary: Endosome-associated protein that plays a role in membrane receptor sorting, cytokinesis and ciliogenesis (PubMed:23108400, PubMed:25278552, PubMed:27767179). Involved in the endosome-to-plasma membrane trafficking and recycling of SNX27-retromer-dependent cargo proteins, such as GLUT1 (PubMed:25278552). Involved in the regulation of cytokinesis; the function may involve PTPN13 and GIT1 (PubMed:23108400). Plays a role in the formation of cilia (PubMed:27767179). Involved in cargo protein localization, such as PKD2, at primary cilia (PubMed:27767179). Involved in the presentation of the tumor necrosis factor (TNF) receptor TNFRSF1A on the cell surface, and hence in the modulation of the TNF-induced apoptosis (By similarity).[UniProtKB/Swiss-Prot Function]