SND1 (NM_014390) Human Tagged Lenti ORF Clone

CAT#: RC200059L4

Lenti ORF clone of Human staphylococcal nuclease and tudor domain containing 1 (SND1), mGFP tagged

Product Images

Circular map for RC200059L4

• 

  Double digestion of RC200059L4 using SgfI and MluI

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: mGFP
• Symbol: SND1
• Synonyms: p100; TDRD11; Tudor-SN
• Vector: pLenti-C-mGFP-P2A-Puro
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: Puromycin
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC200059).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014390
• ORF Size: 2730 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014390.2
• RefSeq Size: 3522 bp
• RefSeq ORF: 2733 bp
• Locus ID: 27044
• UniProt ID: Q7KZF4
• Cytogenetics: 7q32.1
• Domains: TUDOR, SNase, TUDOR
• Protein Families: Druggable Genome, Transcription Factors
• MW: 102 kDa
• Gene Summary: This gene encodes a transcriptional co-activator that interacts with the acidic domain of Epstein-Barr virus nuclear antigen 2 (EBNA 2), a transcriptional activator that is required for B-lymphocyte transformation. Other transcription factors that interact with this protein are signal transducers and activators of transcription, STATs. This protein is also thought to be essential for normal cell growth. A similar protein in mammals and other organisms is a component of the RNA-induced silencing complex (RISC). [provided by RefSeq, Jul 2016]