SNX17 (NM_014748) Human Tagged ORF Clone

CAT#: RC200046

SNX17 (Myc-DDK-tagged)-Human sorting nexin 17 (SNX17)

Product Images

Western blot validation of overexpression lysate (Cat# LY415066) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200046 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SNX17 protein (Cat# TP300046). The protein was produced from HEK293T cells transfected with SNX17 cDNA clone (Cat# RC200046) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SNX17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200046 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACTTTTCCATTCCCGAAACCGAGTCCCGCAGCGGGGACAGCGGCGGCTCCGCCTACGTGGCCTATA
  ACATTCACGTGAATGGAGTCCTGCACTGTCGGGTGCGCTACAGCCAGCTCCTGGGGCTGCACGAGCAGCT
  TCGGAAGGAGTATGGGGCCAATGTGCTTCCTGCATTCCCCCCAAAGAAGCTTTTCTCTCTGACTCCTGCT
  GAGGTAGAACAGAGGAGAGAGCAGTTAGAGAAGTACATGCAAGCTGTTCGGCAAGACCCATTGCTTGGGA
  GCAGCGAGACTTTCAACAGTTTCCTGCGTCGGGCACAACAGGAGACACAGCAGGTCCCCACAGAGGAAGT
  GTCCTTGGAAGTGCTGCTCAGCAACGGGCAGAAAGTTCTGGTCAACGTGCTAACTTCAGATCAGACTGAG
  GATGTCCTGGAGGCTGTAGCTGCAAAGCTGGATCTTCCAGATGACTTGATTGGATACTTTAGTCTATTCT
  TAGTTCGAGAAAAAGAGGATGGAGCCTTTTCTTTTGTACGGAAGTTGCAAGAGTTTGAGCTGCCTTATGT
  GTCTGTCACCAGCCTTCGGAGTCAAGAGTATAAGATTGTGCTAAGGAAGAGTTATTGGGACTCTGCCTAT
  GATGACGATGTCATGGAGAACCGGGTTGGCCTGAACCTGCTTTATGCTCAGACGGTATCAGATATTGAGC
  GTGGGTGGATCTTGGTCACCAAGGAACAGCACCGGCAACTCAAATCTCTGCAAGAGAAAGTCTCCAAGAA
  GGAGTTCCTGAGACTGGCCCAGACGCTGCGGCACTATGGCTACTTGCGCTTTGATGCCTGTGTGGCTGAC
  TTCCCAGAAAAGGACTGTCCTGTGGTGGTGAGCGCGGGCAACAGTGAGCTCAGCCTGCAGCTCCGCCTGC
  CTGGCCAGCAACTCCGAGAAGGCTCCTTCCGGGTCACCCGCATGCGATGCTGGCGGGTCACCTCCTCTGT
  ACCATTGCCCAGTGGAAGCACGAGCAGCCCAGGCCGGGGCCGGGGTGAGGTGCGCCTGGAACTGGCTTTT
  GAATACCTCATGAGCAAGGACCGGCTACAGTGGGTCACCATCACTAGCCCCCAGGCTATCATGATGAGCA
  TCTGCTTGCAGTCCATGGTTGATGAACTGATGGTGAAGAAATCTGGCGGCAGTATCAGGAAGATGCTGCG
  CCGGCGGGTGGGGGGTACTCTGAGACGCTCAGACAGCCAGCAAGCAGTGAAGTCCCCACCACTGCTTGAG
  TCACCTGATGCCACCCGGGAGTCTATGGTCAAACTCTCAAGTAAGCTGAGTGCCGTGAGCTTGCGGGGAA
  TTGGCAGTCCCAGCACAGATGCCAGTGCCAGTGATGTCCACGGCAATTTCGCCTTCGAGGGCATTGGAGA
  TGAGGATCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200046 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MHFSIPETESRSGDSGGSAYVAYNIHVNGVLHCRVRYSQLLGLHEQLRKEYGANVLPAFPPKKLFSLTPA
  EVEQRREQLEKYMQAVRQDPLLGSSETFNSFLRRAQQETQQVPTEEVSLEVLLSNGQKVLVNVLTSDQTE
  DVLEAVAAKLDLPDDLIGYFSLFLVREKEDGAFSFVRKLQEFELPYVSVTSLRSQEYKIVLRKSYWDSAY
  DDDVMENRVGLNLLYAQTVSDIERGWILVTKEQHRQLKSLQEKVSKKEFLRLAQTLRHYGYLRFDACVAD
  FPEKDCPVVVSAGNSELSLQLRLPGQQLREGSFRVTRMRCWRVTSSVPLPSGSTSSPGRGRGEVRLELAF
  EYLMSKDRLQWVTITSPQAIMMSICLQSMVDELMVKKSGGSIRKMLRRRVGGTLRRSDSQQAVKSPPLLE
  SPDATRESMVKLSSKLSAVSLRGIGSPSTDASASDVHGNFAFEGIGDEDL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014748
• ORF Size: 1410 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014748.4
• RefSeq Size: 2475 bp
• RefSeq ORF: 1413 bp
• Locus ID: 9784
• UniProt ID: Q15036
• Cytogenetics: 2p23.3
• Domains: PX
• Protein Families: Druggable Genome
• MW: 52.9 kDa
• Gene Summary: This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein does not contain a coiled coil region, like some family members, but contains a B41 domain. This protein interacts with the cytoplasmic domain of P-selectin, and may function in the intracellular trafficking of P-selectin. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2012]