SNX17 (NM_014748) Human Tagged ORF Clone

CAT#: RC200046

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SNX17 (Myc-DDK-tagged)-Human sorting nexin 17 (SNX17)



  "NM_014748" in other vectors (4)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 457.00

USD 686.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SNX17
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200046 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCACTTTTCCATTCCCGAAACCGAGTCCCGCAGCGGGGACAGCGGCGGCTCCGCCTACGTGGCCTATA
ACATTCACGTGAATGGAGTCCTGCACTGTCGGGTGCGCTACAGCCAGCTCCTGGGGCTGCACGAGCAGCT
TCGGAAGGAGTATGGGGCCAATGTGCTTCCTGCATTCCCCCCAAAGAAGCTTTTCTCTCTGACTCCTGCT
GAGGTAGAACAGAGGAGAGAGCAGTTAGAGAAGTACATGCAAGCTGTTCGGCAAGACCCATTGCTTGGGA
GCAGCGAGACTTTCAACAGTTTCCTGCGTCGGGCACAACAGGAGACACAGCAGGTCCCCACAGAGGAAGT
GTCCTTGGAAGTGCTGCTCAGCAACGGGCAGAAAGTTCTGGTCAACGTGCTAACTTCAGATCAGACTGAG
GATGTCCTGGAGGCTGTAGCTGCAAAGCTGGATCTTCCAGATGACTTGATTGGATACTTTAGTCTATTCT
TAGTTCGAGAAAAAGAGGATGGAGCCTTTTCTTTTGTACGGAAGTTGCAAGAGTTTGAGCTGCCTTATGT
GTCTGTCACCAGCCTTCGGAGTCAAGAGTATAAGATTGTGCTAAGGAAGAGTTATTGGGACTCTGCCTAT
GATGACGATGTCATGGAGAACCGGGTTGGCCTGAACCTGCTTTATGCTCAGACGGTATCAGATATTGAGC
GTGGGTGGATCTTGGTCACCAAGGAACAGCACCGGCAACTCAAATCTCTGCAAGAGAAAGTCTCCAAGAA
GGAGTTCCTGAGACTGGCCCAGACGCTGCGGCACTATGGCTACTTGCGCTTTGATGCCTGTGTGGCTGAC
TTCCCAGAAAAGGACTGTCCTGTGGTGGTGAGCGCGGGCAACAGTGAGCTCAGCCTGCAGCTCCGCCTGC
CTGGCCAGCAACTCCGAGAAGGCTCCTTCCGGGTCACCCGCATGCGATGCTGGCGGGTCACCTCCTCTGT
ACCATTGCCCAGTGGAAGCACGAGCAGCCCAGGCCGGGGCCGGGGTGAGGTGCGCCTGGAACTGGCTTTT
GAATACCTCATGAGCAAGGACCGGCTACAGTGGGTCACCATCACTAGCCCCCAGGCTATCATGATGAGCA
TCTGCTTGCAGTCCATGGTTGATGAACTGATGGTGAAGAAATCTGGCGGCAGTATCAGGAAGATGCTGCG
CCGGCGGGTGGGGGGTACTCTGAGACGCTCAGACAGCCAGCAAGCAGTGAAGTCCCCACCACTGCTTGAG
TCACCTGATGCCACCCGGGAGTCTATGGTCAAACTCTCAAGTAAGCTGAGTGCCGTGAGCTTGCGGGGAA
TTGGCAGTCCCAGCACAGATGCCAGTGCCAGTGATGTCCACGGCAATTTCGCCTTCGAGGGCATTGGAGA
TGAGGATCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200046 protein sequence
Red=Cloning site Green=Tags(s)

MHFSIPETESRSGDSGGSAYVAYNIHVNGVLHCRVRYSQLLGLHEQLRKEYGANVLPAFPPKKLFSLTPA
EVEQRREQLEKYMQAVRQDPLLGSSETFNSFLRRAQQETQQVPTEEVSLEVLLSNGQKVLVNVLTSDQTE
DVLEAVAAKLDLPDDLIGYFSLFLVREKEDGAFSFVRKLQEFELPYVSVTSLRSQEYKIVLRKSYWDSAY
DDDVMENRVGLNLLYAQTVSDIERGWILVTKEQHRQLKSLQEKVSKKEFLRLAQTLRHYGYLRFDACVAD
FPEKDCPVVVSAGNSELSLQLRLPGQQLREGSFRVTRMRCWRVTSSVPLPSGSTSSPGRGRGEVRLELAF
EYLMSKDRLQWVTITSPQAIMMSICLQSMVDELMVKKSGGSIRKMLRRRVGGTLRRSDSQQAVKSPPLLE
SPDATRESMVKLSSKLSAVSLRGIGSPSTDASASDVHGNFAFEGIGDEDL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_014748
ORF Size 1410 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_014748.4
RefSeq Size 2475 bp
RefSeq ORF 1413 bp
Locus ID 9784
UniProt ID Q15036
Cytogenetics 2p23.3
Domains PX
Protein Families Druggable Genome
MW 52.9 kDa
Gene Summary This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein does not contain a coiled coil region, like some family members, but contains a B41 domain. This protein interacts with the cytoplasmic domain of P-selectin, and may function in the intracellular trafficking of P-selectin. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2012]

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