Ncor2 (NM_001253904) Mouse Tagged ORF Clone

CAT#: MR231943

Ncor2 (myc-DDK-tagged) - Mouse nuclear receptor co-repressor 2 (Ncor2), transcript variant 2

Product Images

Circular map for MR231943

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ncor2
• Synonyms: N-CoR; SMRT; SMRTe
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231943 representing NM_001253904
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCGCGTGGACCGTGAGATCACCATGGTAGAGCAGCAGATCTCCAAGCTGAAGAAGAAGCAGCAAC
  AGTTGGAGGAGGAGGCCGCCAAGCCGCCCGAACCCGAGAAGCCTGTGTCGCCACCACCCATAGAATCAAA
  GCACCGAAGCCTGGTCCAGATCATCTACGATGAGAACCGGAAGAAAGCCGAAGCCGCACACCGGATCCTA
  GAAGGCCTGGGGCCCCAGGTGGAGCTGCCTCTGTACAACCAGCCGTCTGACACACGCCAGTACCATGAAA
  ACATCAAAATAAACCAGGCGATGCGGAAGAAGCTGATCTTGTACTTTAAGCGGAGGAACCACGCGCGCAA
  GCAGTGGGAACAGCGCTTCTGCCAGCGCTATGACCAGCTCATGGAGGCGTGGGAGAAGAAGGTAGAGCGC
  ATAGAGAACAATCCGCGAAGGAGGGCCAAGGAGAGCAAGGTGAGGGAGTACTACGAGAAACAGTTCCCGG
  AGATCCGCAAGCAGCGGGAGCTGCAGGAGCGCATGCAGAGCAGGGTGGGCCAGCGTGGCAGTGGGCTCTC
  CATGTCGGCTGCCCGCAGTGAGCATGAGGTTTCTGAGATCATTGATGGCTTGTCTGAGCAGGAGAATCTG
  GAGAAGCAGATGCGCCAGCTGGCCGTGATCCCGCCCATGTTGTACGACGCGGACCAGCAGAGGATCAAGT
  TCATCAACATGAATGGACTCATGGATGACCCCATGAAGGTCTACAAGGACCGTCAGGTTACCAACATGTG
  GAGCGAGCAGGAGAGGGACACCTTCCGTGAGAAGTTTATGCAGCACCCTAAGAACTTTGGCCTGATTGCC
  TCATTCCTGGAGAGAAAGACGGTCGCTGAGTGTGTCCTCTATTACTACCTGACCAAGAAGAATGAAAATT
  ACAAGAGCTTGGTGAGGCGGAGCTATCGGCGCCGTGGCAAGAGCCAGCAGCAGCAGCAGCAGCAACAACA
  GCAGCAGCAGCAGCAGATGGCACGGAGCAGCCAGGAGGAGAAGGAGGAGAAGGAGAAGGAGAAGGAGGCC
  GACAAGGAGGAAGAGAAGCAGGATGCGGAGAACGAGAAGGAAGAACTCAGCAAGGAGAAGACAGACGACA
  CTTCTGGCGAGGACAACGATGAGAAAGAGGCCGTGGCCTCCAAAGGCCGCAAAACTGCCAACAGCCAAGG
  CCGCCGCAAAGGCCGTATCACGCGCTCCATGGCCAACGAGGCCAACCATGAGGAGACAGCCACCCCACAG
  CAAAGTTCAGAGCTGGCTTCCATGGAGATGAACGAGAGTTCTCGCTGGACTGAGGAAGAGATGGAGACAG
  CAAAGAAAGGCCTCCTGGAACATGGGAGGAACTGGTCAGCCATTGCCCGTATGGTGGGCTCCAAGACCGT
  GTCCCAGTGTAAGAACTTCTACTTCAACTACAAGAAGAGGCAGAACCTGGACGAAATCCTTCAGCAGCAC
  AAGCTAAAGATGGAGAAGGAGAGGAACGCTCGGAGGAAGAAGAAGAAGACCCCAGCTGCGGCGAGCGAGG
  AGACAGCCTTCCCACCTGCCGCTGAGGACGAAGAGATGGAAGCATCAGGCGCAAGTGCCAATGAGGAAGA
  GCTGGCGGAGGAGGCAGAAGCCTCACAGGCCTCTGGGAATGAGGTTCCCAGAGTTGGGGAGTGCAGTGGC
  CCAGCTGCTGTCAACAACAGCTCTGATACTGAGAGTGTCCCATCCCCGCGTTCAGAAGCCACGAAGGACA
  CTGGGCCTAAACCCACTGGCACTGAAGCATTGCCCGCTGCCACCCAGCCACCTGTTCCTCCTCCAGAAGA
  ACCGGCAGTAGCCCCTGCTGAGCCCTCCCCAGTCCCTGATGCCAGTGGCCCACCATCCCCAGAGCCTTCC
  CCATCACCTGCCGCACCCCCGGCTACTGTGGACAAGGATGAACAAGAAGCCCCGGCTGCTCCAGCTCCCC
  AGACAGAGGATGCCAAGGAGCAGAAGTCTGAGGCCGAGGAGATCGATGTGGGAAAGCCAGAGGAGCCCGA
  GGCCTCTGAGGAGCCCCCGGAGAGTGTAAAGAGTGACCACAAGGAGGAGACCGAGGAAGAGCCTGAAGAC
  AAAGCCAAGGGCACAGAGGCCATTGAAACTGTGTCTGAGGCACCACTTAAGGTGGAGGAGGCTGGTAGCA
  AGGCAGCTGTGACCAAGGGTTCCAGCTCAGGTGCCACCCAGGACAGTGACTCCAGTGCCACCTGCAGTGC
  CGATGAGGTGGACGAACCCGAAGGAGGTGACAAGGGCAGGCTGCTGTCACCAAGGCCCAGCCTCCTCACC
  CCGGCTGGAGATCCCCGGGCCAGTACCTCGCCCCAGAAGCCGCTGGACCTGAAGCAGCTGAAGCAGCGAG
  CAGCCGCCATCCCCCCTATCGTCACCAAGGTCCATGAGCCCCCCCGGGAGGACACAGTACCCCCAAAGCC
  AGTTCCCCCTGTGCCTCCACCCACGCAGCACCTACAGCCAGAGGGTGACGTGTCTCAGCAGTCGGGAGGA
  AGTCCACGTGGCAAGTCCCGCAGCCCAGTGCCTCCTGCCGAGAAAGAGGCAGAGAAACCCGCATTCTTTC
  CGGCTTTCCCAACTGAGGGCCCAAAGCTACCGACTGAGCCCCCACGCTGGTCATCGGGCCTGCCCTTCCC
  CATCCCTCCACGGGAGGTGATCAAGACTTCCCCACACGCCGCTGACCCCTCTGCCTTCTCCTACACACCC
  CCCGGTCACCCGCTGCCTCTGGGCCTCCACGATAGTGCCCGGCCCGTCCTGCCACGTCCCCCCATCTCTA
  ACCCCCCACCCCTCATCTCCTCTGCCAAGCATCCCGGCGTACTTGAGAGGCAGCTGGGTGCCATCTCCCA
  GCAGGGGATGTCAGTCCAGCTTCGTGTGCCTCACTCAGAGCATGCCAAGGCCCCCATGGGCCCTCTCACC
  ATGGGGCTGCCCCTTGCCGTGGACCCTAAGAAGCTGGCCCCCTTCAGTGGAGTGAAGCAGGAACAGTTGT
  CCCCTCGGGGTCAGGCTGGGCCACCTGAAAGTCTGGGAGTGCCCACTGCTCAGGAGACCTCTGTGCTGAG
  AGGGACAGCACTGGGTTCCGCCACCAGTGGAAGCATCACCAAGGGCCTCCCCAGTACCCGGGCTGCAGAC
  GGCCCCAGCTACAGAGGCTCTATCACCCACGGCACGCCCGCAGACGTCCTCTACAAGGGTACCATCAGCA
  GGATCGTCGGTGAGGACAGCCCAAGTCGCCTTGACCGGGCACGAGAGGACACCCTGCCCAAGGGCCATGT
  CATCTATGAGGGCAAGAAAGGCCACGTCCTATCCTATGAAGGTGGTATGTCCGTGTCACAGTGCTCTAAG
  GAGGATGGAAGGAGCAGCTCGGGCCCACCCCATGAGACTGCCGCCCCTAAACGCACCTATGACATGATGG
  AGGGCCGTGTAGGCAGGACTGTCACCTCAGCCAGCATAGAGGGACTCATGGGCCGCGCCATCCCTGAGCA
  GCACAGCCCCCACCTCAAGGAGCAGCATCACATCCGAGGCTCCATCACGCAAGGCATCCCGAGGTCCTAT
  GTGGAGGCGCAGGAGGACTACTTACGGCGGGAGGCCAAGCTCTTGAAGCGAGAAGGGACACCACCACCCC
  CACCACCACCTCGGGACCTGACTGAGACCTACAAGCCCCGGCCCCTGGACCCTCTGGGTCCCCTGAAGCT
  GAAGCCGACTCACGAGGGTGTGGTAGCAACTGTGAAGGAGGCGGGCCGCTCTATCCATGAGATCCCGAGA
  GAGGAGCTGCGCCGCACACCTGAGCTACCCCTGGCACCACGGCCTCTGAAGGAGGGTTCCATCACCCAGG
  GCACCCCACTCAAGTACGACTCTGGGGCACCCTCCACTGGCACCAAGAAACACGACGTGCGCTCCATCAT
  CGGCAGCCCCGGCCGGCCTTTCCCTGCCCTGCACCCGCTGGACATAATGGCTGACGCCCGGGCACTGGAG
  CGTGCCTGCTATGAAGAGAGTCTGAAGAGCCGGTCAGGGACCAGCAGTGGTGCAGGGGGCTCCATCACAC
  GTGGGGCTCCAGTCGTCGTGCCTGAACTGGGCAAGCCACGGCAAAGCCCACTGACTTACGAAGACCACGG
  GGCACCCTTCACCAGTCACCTGCCACGTGGCTCCCCTGTGACCACGAGGGAGCCCACGCCACGCCTTCAG
  GAAGGCAGCCTCCTATCCAGCAAGGCGTCCCAGGACCGGAAGCTGACATCTACACCCCGGGAGATCGCCA
  AGTCCCCACACAGCACTGTGCCCGAGCACCACCCTCACCCCATCTCCCCCTATGAGCACTTGCTCCGGGG
  CGTGACTGGTGTGGACCTGTACCGTGGTCACATCCCATTGGCCTTTGACCCCACCTCCATACCCCGAGGG
  ATCCCTCTGGAAGCAGCAGCCGCAGCCTACTACCTGCCCCGGCACTTGGCCCCCAGCCCCACCTACCCAC
  ACCTGTACCCACCTTACCTCATCCGCGGCTACCCTGACACGGCGGCCCTGGAGAACCGCCAGACCATCAT
  CAATGACTACATCACCTCGCAGCAGATGCACCACAACGCTGCCTCCGCCATGGCCCAGCGTGCTGACATG
  CTGAGGGGTCTGTCACCGCGAGAGTCCTCGCTGGCCCTCAATTATGCCGCTGGCCCAAGAGGCATTATCG
  ACCTGTCCCAAGTGCCACACCTGCCCGTGCTGGTGCCACCAACGCCAGGCACCCCTGCCACCGCCATCGA
  CCGCCTTGCCTACCTCCCCACTGCGCCCCCACCCTTCAGCAGCCGCCACAGTAGCTCACCGCTGTCCCCA
  GGAGGCCCCACTCACCTAGCTAAACCAACTGCCACATCTTCATCGGAGCGGGAACGGGAACGTGAGCGGG
  AACGAGACAAGTCCATCCTCACGTCTACCACTACAGTGGAGCATGCACCCATCTGGAGACCTGGTACGGA
  GCAGAGCAGCGGGGCTGGGGGCAGCAGCCGCCCCGCCTCCCACACCCACCAGCACTCGCCCATCTCCCCC
  CGGACCCAGGACGCCTTGCAGCAGAGGCCCAGTGTGCTGCACAACACGAGCATGAAGGGCGTGGTCACCT
  CCGTGGAACCCGGCACGCCCACGGTCCTGAGGTGGGCCAGGTCCACCTCCACCTCTTCGCCTGTCCGCCC
  AGCTGCCACATTCCCACCTGCCACCCACTGCCCACTTGGTGGCACCCTTGAAGGGGTCTACCCTACCCTC
  ATGGAGCCCGTCCTGTTACCCAAGGAGACCTCTCGGGTCGCCCGGCCCGAGCGGCCCCGTGTGGACGCTG
  GCCATGCCTTCCTCACCAAACCCCCGGCCCGGGAGCCCGCCTCCTCACCCAGCAAGAGCTCCGAGCCCCG
  ATCCCTAGCACCCCCCAGCTCCAGCCACACAGCCATCGCCCGCACCCCAGCAAAGAACCTTGCACCCCAC
  CATGCCAGTCCGGACCCGCCGGCGCCCACCTCGGCCTCAGATCTGCACCGAGAAAAGACTCAAAGTAAAC
  CCTTTTCCATCCAGGAATTGGAACTCCGTTCTCTGGGTTACCACAGTGGAGCTGGCTACAGCCCCGATGG
  GGTGGAGCCCATCAGCCCGGTGAGCTCCCCCAGCCTGACCCACGACAAGGGGCTCTCCAAACCTCTGGAA
  GAGCTAGAGAAGAGCCACTTGGAAGGGGAGCTGCGGCACAAGCAGCCAGGCCCCATGAAGCTCAGCGCGG
  AGGCTGCCCATCTCCCACATCTGCGGCCACTGCCCGAGAGCCAGCCCTCATCTAGCCCACTCCTCCAGAC
  TGCCCCAGGCATCAAAGGTCACCAGAGGGTGGTCACCTTGGCTCAGCACATCAGTGAGGTCATTACGCAG
  GACTACACGCGCCACCACCCGCAGCAGCTCAGTGGCCCCCTTCCCGCCCCTCTCTACTCCTTTCCCGGAG
  CCAGCTGCCCTGTCCTGGATCTTCGCCGCCCACCCAGTGACCTCTACCTCCCACCCCCCGACCATGGCAC
  CCCAGCCCGGGGATCCCCCCACAGTGAAGGGGGCAAAAGGTCCCCAGAACCCAGCAAAACATCGGTCCTG
  GGCAGCAGCGAGGATGCCATTGAGCCTGTGTCCCCACCAGAGGGCATGACTGAGCCAGGACATGCTCGGA
  GCACTGCGTACCCACTGCTGTATCGAGACGGGGAACAGGGCGAGCCCAGGATGGGCTCTAAGTCTCCAGG
  CAACACCAGCCAGCCGCCAGCCTTCTTCAGTAAGCTGACTGAGAGCAACTCCGCCATGGTGAAGTCGAAG
  AAGCAGGAGATCAACAAGAAACTCAACACCCACAACCGGAACGAGCCAGAATACAATATTGGCCAGCCTG
  GGACGGAAATCTTCAACATGCCCGCCATCACTGGAGCAGGCCTTATGACCTGTAGAAGCCAGGCGGTGCA
  AGAACACGCCAGCACCAACATGGGGCTAGAGGCCATTATTAGAAAGGCACTCATGGGTAAATATGATCAG
  TGGGAAGAGCCCCCGCCGCTCGGCGCCAATGCTTTTAACCCTCTGAATGCCAGCGCCAGTCTGCCCGCTG
  CTGCTATGCCCATAACCACTGCTGACGGACGGAGTGACCACGCACTCACCTCGCCAGGTGGAGGTGGGAA
  AGCCAAGGTCTCTGGCAGACCTAGCAGCCGAAAAGCCAAGTCGCCAGCACCAGGCCTAGCGTCCGGAGAC
  CGACCCCCTTCTGTCTCCTCAGTACACTCAGAGGGGGACTGCAATCGCCGAACACCACTCACCAACCGTG
  TGTGGGAGGACCGGCCCTCATCTGCAGGGTCCACGCCATTCCCCTACAACCCTTTGATTATGAGGCTACA
  GGCAGGTGTCATGGCCTCCCCGCCCCCACCTGGCCTTGCGGCAGGCAGCGGGCCCCTAGCTGGTCCCCAC
  CACGCCTGGGATGAGGAGCCCAAGCCACTGCTGTGTTCACAGTATGAGACACTCTCGGACAGCGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231943 representing NM_001253904
  Red=Cloning site Green=Tags(s)
  
  MDRVDREITMVEQQISKLKKKQQQLEEEAAKPPEPEKPVSPPPIESKHRSLVQIIYDENRKKAEAAHRIL
  EGLGPQVELPLYNQPSDTRQYHENIKINQAMRKKLILYFKRRNHARKQWEQRFCQRYDQLMEAWEKKVER
  IENNPRRRAKESKVREYYEKQFPEIRKQRELQERMQSRVGQRGSGLSMSAARSEHEVSEIIDGLSEQENL
  EKQMRQLAVIPPMLYDADQQRIKFINMNGLMDDPMKVYKDRQVTNMWSEQERDTFREKFMQHPKNFGLIA
  SFLERKTVAECVLYYYLTKKNENYKSLVRRSYRRRGKSQQQQQQQQQQQQQQMARSSQEEKEEKEKEKEA
  DKEEEKQDAENEKEELSKEKTDDTSGEDNDEKEAVASKGRKTANSQGRRKGRITRSMANEANHEETATPQ
  QSSELASMEMNESSRWTEEEMETAKKGLLEHGRNWSAIARMVGSKTVSQCKNFYFNYKKRQNLDEILQQH
  KLKMEKERNARRKKKKTPAAASEETAFPPAAEDEEMEASGASANEEELAEEAEASQASGNEVPRVGECSG
  PAAVNNSSDTESVPSPRSEATKDTGPKPTGTEALPAATQPPVPPPEEPAVAPAEPSPVPDASGPPSPEPS
  PSPAAPPATVDKDEQEAPAAPAPQTEDAKEQKSEAEEIDVGKPEEPEASEEPPESVKSDHKEETEEEPED
  KAKGTEAIETVSEAPLKVEEAGSKAAVTKGSSSGATQDSDSSATCSADEVDEPEGGDKGRLLSPRPSLLT
  PAGDPRASTSPQKPLDLKQLKQRAAAIPPIVTKVHEPPREDTVPPKPVPPVPPPTQHLQPEGDVSQQSGG
  SPRGKSRSPVPPAEKEAEKPAFFPAFPTEGPKLPTEPPRWSSGLPFPIPPREVIKTSPHAADPSAFSYTP
  PGHPLPLGLHDSARPVLPRPPISNPPPLISSAKHPGVLERQLGAISQQGMSVQLRVPHSEHAKAPMGPLT
  MGLPLAVDPKKLAPFSGVKQEQLSPRGQAGPPESLGVPTAQETSVLRGTALGSATSGSITKGLPSTRAAD
  GPSYRGSITHGTPADVLYKGTISRIVGEDSPSRLDRAREDTLPKGHVIYEGKKGHVLSYEGGMSVSQCSK
  EDGRSSSGPPHETAAPKRTYDMMEGRVGRTVTSASIEGLMGRAIPEQHSPHLKEQHHIRGSITQGIPRSY
  VEAQEDYLRREAKLLKREGTPPPPPPPRDLTETYKPRPLDPLGPLKLKPTHEGVVATVKEAGRSIHEIPR
  EELRRTPELPLAPRPLKEGSITQGTPLKYDSGAPSTGTKKHDVRSIIGSPGRPFPALHPLDIMADARALE
  RACYEESLKSRSGTSSGAGGSITRGAPVVVPELGKPRQSPLTYEDHGAPFTSHLPRGSPVTTREPTPRLQ
  EGSLLSSKASQDRKLTSTPREIAKSPHSTVPEHHPHPISPYEHLLRGVTGVDLYRGHIPLAFDPTSIPRG
  IPLEAAAAAYYLPRHLAPSPTYPHLYPPYLIRGYPDTAALENRQTIINDYITSQQMHHNAASAMAQRADM
  LRGLSPRESSLALNYAAGPRGIIDLSQVPHLPVLVPPTPGTPATAIDRLAYLPTAPPPFSSRHSSSPLSP
  GGPTHLAKPTATSSSERERERERERDKSILTSTTTVEHAPIWRPGTEQSSGAGGSSRPASHTHQHSPISP
  RTQDALQQRPSVLHNTSMKGVVTSVEPGTPTVLRWARSTSTSSPVRPAATFPPATHCPLGGTLEGVYPTL
  MEPVLLPKETSRVARPERPRVDAGHAFLTKPPAREPASSPSKSSEPRSLAPPSSSHTAIARTPAKNLAPH
  HASPDPPAPTSASDLHREKTQSKPFSIQELELRSLGYHSGAGYSPDGVEPISPVSSPSLTHDKGLSKPLE
  ELEKSHLEGELRHKQPGPMKLSAEAAHLPHLRPLPESQPSSSPLLQTAPGIKGHQRVVTLAQHISEVITQ
  DYTRHHPQQLSGPLPAPLYSFPGASCPVLDLRRPPSDLYLPPPDHGTPARGSPHSEGGKRSPEPSKTSVL
  GSSEDAIEPVSPPEGMTEPGHARSTAYPLLYRDGEQGEPRMGSKSPGNTSQPPAFFSKLTESNSAMVKSK
  KQEINKKLNTHNRNEPEYNIGQPGTEIFNMPAITGAGLMTCRSQAVQEHASTNMGLEAIIRKALMGKYDQ
  WEEPPPLGANAFNPLNASASLPAAAMPITTADGRSDHALTSPGGGGKAKVSGRPSSRKAKSPAPGLASGD
  RPPSVSSVHSEGDCNRRTPLTNRVWEDRPSSAGSTPFPYNPLIMRLQAGVMASPPPPGLAAGSGPLAGPH
  HAWDEEPKPLLCSQYETLSDSE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001253904
• ORF Size: 6996 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001253904.1, NP_001240833.1
• RefSeq Size: 8697 bp
• RefSeq ORF: 6999 bp
• Locus ID: 20602
• UniProt ID: Q9WU42
• Cytogenetics: 5 G1.1
• MW: 254.3 kDa
• Gene Summary: Transcriptional corepressor. Mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription. Isoform 1 and isoform 5 have different affinities for different nuclear receptors. Involved in the regulation BCL6-dependent of the germinal center (GC) reactions, mainly through the control of the GC B-cells proliferation and survival. Recruited by ZBTB7A to the androgen response elements/ARE on target genes, negatively regulates androgen receptor signaling and androgen-induced cell proliferation.[UniProtKB/Swiss-Prot Function]