Nin (NM_001286080) Mouse Tagged ORF Clone

CAT#: MR231905

Nin (myc-DDK-tagged) - Mouse ninein (Nin), transcript variant 4

Product Images

Circular map for MR231905

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nin
• Synonyms: 3110068G20Rik; AI385615; AU024711; mKIAA1565
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231905 representing NM_001286080
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGAGGTGGAGGAGGACCAGCATGAAGCCCGACTCAAGGAGCTGTTTGACAGTTTTGACACCCTGG
  GAACTGGGTCTCTGGGTCAAGAGGAGCTCACTGACCTCTGCCACGTGCTGTGCTTGGAGGATGTGGGCCC
  GGTGCTGCAACAGACGCTACTCCAGGACAACCTCTTGGGCAGGGTACATTTTGACCAATTTAAAGAAGCA
  TTAATACTTATCTTGTCTAGAACTCTATCCAGTGAGGAGCACTTTGAGGAGTCAGACTGTTCCCCAGAAG
  CTCAGCCCAAATATGTTAGAGGTGGGAAGCGTTATGGACGAAGATCACTGCCCGAGTTTCAAGAGTCTGG
  GGAAGAGATCGAGGAGGTGACAGTGCTTGAGCCACTAGAGGAAGAAGCGAGGTCATCACCCATCCCAGCT
  GGGGACTGCGGCGAGCACTGGAAGACACAACGCAGTGAGGAGTATGAAGCAGAAGGCCAGCTGAGGTTTT
  GGAACCCAGATGACCTGAATGCTTCACATGGTGGGTCTTGCCCTCCTCCAGACTGGATAGAAGAGAAACT
  GCAAGAGGTTTGTGAAGATCTGGGGATCACTCGAGATGGTCACCTGAACCGGAAGAAGCTGGTTTCCATC
  TGCGAACAGTATGGATTACAGAATGTGGATGGAGCGATGCTGGAAGAGGTGTTTCTCAGCCTTGATCCTG
  ATGGAACAATGAGTGTGGAAGACTTTTTCTATGGTCTGTTTAAGACTGGGAAATCCCTCACGCCATCAGC
  ATCTACTCCCTATAGACAACTGAAACGGCATCTCTCTATGCAGTCTTTCGATGAGAGTGGACGGCGGACC
  GCAACCTCGTCAGCAATGACGAGTACCATTGGCTTCCGGGTCTTCTCCTGCTTGGATGACGGGATGGGCC
  AGGCGTCTGTGGAGAGGATACTGGACACCTGGCAGGAAGAAGGCATTGAGAACAGTCAGGAGATTCTGAA
  GGCCCTGGATTTCAGCCTGGATGGAAACATCAACCTGACAGAGCTGACGCTGGCTCTGGAGAATGAACTT
  TTGGTTACCAAGAATGGCATTCACCAGGCAGCACTAGCTAGCTTCAAGGCCGAGATCCGGCACTTGTTAG
  AACGAGTTGACCAAGTAGTCAGAGAAAAGGAGAAGCTACGGTCGGACCTGGACAAAGCAGAAAAGCTGAA
  GTCTCTCATGGCTTCCGAGGTGGACGATCACCACGCGGCCATTGAGCGGAGGAATGAATACAACCTCAGG
  AAACTGGATGAAGAATACAAGGAGCGCATAGCAGCCTTAAAGAACGAACTCCGCCAGGAGAGGGAACAGA
  TGTTGCAGCAAGTGGGCAAGCAGCGGGTGGAGCTGGAACAGGAAATTCAAAAGGCCAAGACGGAAGAGAA
  TTACATCCGGGACCGCCTTGCCCTCTCCCTGAAGGAAAACAATCGCCTGGAGACTGAGCTTCTGGAAAAC
  GCAGAGAAGTTGGCAGAGTACGAGAGCCTGACGCAGAAGCTCCAGCGCAGTTTGGAAAATGTGTTAGCAG
  AAAAGTTTGGTGACCTCGATCCCAGCAGTGCTGAGTTCTTTCTTCAAGAGGAGAGGCTGGCGCAGATGAG
  AAATGAATATGAGCAACAGTGCAGGCTATTACAAGACCAAGTGGATGAGCTTCAGTCTGAACTAGAAGAA
  TACCAGGCTCAAGGCAGGGTGCTCAGACTCCCCTTGAAGAACTCGCTGTCAGAAGAACTCGACGGTCACA
  GTGGTGGTATTGAGCCCGACCAAGGGCCTGGATCTGAAGAGTGCAACCCCCTGAATATGAGCATTGAGGC
  AGAGCTGGTCATCGAGCAGATGAAGGAGCAGCACCACCGGGACCTGTGTCACCTGAGACTGGAGCTGGAA
  GACAAAGTGCGCCACTATGAAAAGCAGCTGGATGATACCAGGGTCGCCTCTGAACAGGAGCAGGCAGCCA
  TGAAGCAGAAGTATGAGCAGGGAGTGCACACCTTGGAGAAGCGAGTCAGCGAGCTTCGGAGTGAGATTGC
  AGACCTGGAAGGGCAGGCAGCCGTGCTGAGGGAGGCCCACCACAAGGCCAGCTGCAGGCACGAGGAGGAA
  AAGAGACAACTGCAAATGGCGTTCGATGAGGAAAAGGCTCAGCTCCAGGAGGAGCTGCGGCAGGAGCATG
  AGAGGGAGCTCCAGGCCAGGCTGCAGCAGGCAGCGGAGAGCTTTCGCCAGGAGAGAGAGGGACTCGCCCA
  GGCTGCCTGGACTGAGGAGAAGGTGAGAGGCTTGGAGCAATCTTACCAGGAGCAGCTGCTGAGCCTGGAG
  GAGAAGCATGCTCTAGAGAAAGAAGAGCTGAGGGAAGAGCTCTCGGAACATCACCGAAGGGAACTCCAGG
  AGGGAAGGGAAGAAATGGAAACAGAGTGTAATAGAAGAGTCTCTCAGATAGAAGCCCAGTGCCAGGCTGA
  CTGTGAGAAAGTCACTGAGCACTGTGAACAAACTCTGCAAAGCCTCGAGGTTCGCCACCGCCAGGAGCTG
  AGGGACCTCTTGGACCAGCACCTGGAGGAAAGGTCTCAGTGGGAGTTTGAGAAGGATGAGCTTACACAAG
  AGTGCACAGATGCCCAGGAGCAGCTGAAGGAGGCGCTCCAGAGGGAGAGGGCGACCGCTGCGGCCATGAA
  GCAGGAACAGGAGATACTGGAGAGGACATACAAGGACCGTTTGAATATCCTGAGCACGGAAAGAAAGCAG
  CTGCTGCAAGACCTGAAAGACCTACAGAACGCATCTGAGAGCCAGCATGGCCTCCTGTCTGGCCAGATAC
  TTGAGCTGAAGAGAAGTCAGGAGAGAGAACTGAGGGACCAAGGGCAGGCGCTATGCCAGACGGGGGTCTC
  TGAGCAGCTGGCCAGCCAGCAGCTCGAAAGGCTCCGGGTTGAGCATGAGCAAGAGAGGCGAGAAATGACG
  GGCAAGCTTGCCGCCCTGGAGAGCGCCCACAGAGCAAGCCTTGAGAGAGCAGATCAAGAGAAGGCGGAGA
  TGAGCACAGAAATCTGCAGGCTTCAGAATACAGTCAAGGACATGCAGCAGGCAGCATCTCTCTTGATGCT
  TCAGGGTGGCTGCCAGGCAACGGCAGGGGAGGAGGCAGAAGGAGATGGAGCCATGTCCCTGCTTCAGCAA
  GGCGAGCAGCTATTGGAAGAAAACGGAGATGTCCTCATCAGCCTGCAGAGAGCTCACGAGCATGCAGTGA
  AGGAAAATGCAAAGATGGCTACTGAAATTTCCCGATTGCAGCAGAGGCTGAAGAAGTTAGAGCCGGGGTC
  GGTGATCTCGTCTTGTTTGGAGGAGGGGACGTCTGAGATTTCTGGAAGTTCCAGGGAACAAGTAGAACCC
  ATAATGAAACAAGGTCCAGCCACCAAGCACTTCCTGAGCGATCTAGGAGACCACGAGGCCCGGGACCTGG
  CAAGTACCGGGACAAGCTCCGTTCAGAGGCAGGAGTGCAAAACCGAGGCGTCGGAAGCTTCCCTGGACTG
  CTTTTCTGAGCTTGAAAACAGTGAAGACACCAGGACCGAGTCCTGGGACCTCAAGAGTCAGATCAGCCAG
  CTTCGGGAGCAACTGACGGTCTTGCGAGCAGACTGCGATCGGGCTTCCGAGAGGAAACAGGACCTGCTTT
  TCGATATCTCTGTGCTGAAAAAGAAACTGAAGATGCTGGAGAGACTCCCGGAGGCATCTTCCAGGTATAA
  GGTATTATACGAGGATGCCGCCCGAGAAAACTCCTGCCTTCAGGAGGAGCTGAGACTTGTAGAGACGCGC
  TACGAGGAGTCGCTGGACAGCAACAAAGAGCTTACGGCGGAGGTCTACAGGTTGCAGGATGAGATGAAGA
  AAATGGAGGAGGTGATGGAGACGTTCCTCAGCCTGGAGAAGAGTTACGATGAGGTCAAAGTGGAAAATGA
  GGAACTCCGTGCTCTGGTGTTGAGACTTCAAGGGAAGATGGAGAAGGTGCTGGGAAGGGCCGCCCTGCAG
  GGTGACAGCTACGCCCTCTGGGAAGCCCCTTCAGAAAACCTGGAGGTCGCCTCGGATGAAAAGATGCTTG
  AACTCCGTCAGACTCCGAAAGAGTGTACGCCCAAGGTCGTGAGCATGCACCACATCATAGAAGAGTGTAC
  ACAAGAAACCCAGTGCTGTGAGCAGGGAAGCACGAAGCTCCTAGCTAGAATAAAAGCACACGAAATCGCC
  TGGTTCCACAGAGCAATTAAGACACATCCGGAAAAGCCTAGCGCGCAGAACCGAGTCATCCCGGAGGGAA
  GTGCTGCTCTCCTAGGCCTACAAGACAAGCATCTTCAGCAGGAGGCCACCATCGCAGAGTTAGAACTAGA
  GAAGCAAAAGCTACAGGAGCTGACTAGAAATCTGAGGGAGCGAGTCACTGCACTAGTTAGGCAAAAAGAC
  GCCCCTTCTCAAGGGCAGAAGGAGGAGGAGCTGAAGGCAATGATGCAGGACCTGCAGATCACGTGCGGGG
  AGATGCAGAGGAAGGTTGAACTCCTGAGATATGAATCTGAAAAGCTTCAAGAGGAAAATTCTATTTTGAG
  AAATGAAATTACTACTTTAAATGAAGAAGATAGCATCTCTAACCTGAAATTGGAGGAATTAAATGGCTCT
  CAGGAAGAATTGTGGCAGAAAATAGAAACCATAGAGCAGGAAAAAGCTTCAATTCAGACAATGGTTGAAA
  AATTAAAGAAACAGGTTTCAGATTTAAAAATCAAAAACCAACAGTTGGATTCAGAAAATATAGAACTCAG
  CCAAAAGAACTCCCAAAACAAGGAAGAACTGAAAACCCTTAATCAACGTCTGGCAGAAATGCTGTGCCAG
  AGGGAGGAGCCAGGAGCTTGCACCTCTGAGAAATGGGAACAAGAAAATGCAAGCCTCAAAGAAGAACTGG
  ATCACTACAAAGTGCAGACTTCCACTTTGGTGTCTTCTCTGGAGGCAGAACTTTCCGAAGTTAAACTGCA
  GACTCACGTCATGGAGCAGGAAAACCTCCTTCTCAAAGATGAACTGGAGAGACTGAAGCAACTGCACAGA
  TGTCCTGACCTCTCTGACTTCCAGCAAAAAATGTCTAGCATTCTAAGCTACAATGAAAAACTGCTGAAGG
  AAAAAGAAGTTCTGAGTGAAGAGTTAAAGAGCTGTGCAGATAAGCTGGCAGAGTCAAGCCTCTTAGAGCA
  CAGAATTGCTACAATGAAGCAGGAGCAGACCGCCTGGGAAGAGCAGAGTGAGAGCCTGAAGTCACAGCTG
  GCAGTGTCGCAGGCAAAGGTTCAGAACTTAGAAGATGTCCTGCAGAATGTCAACCTTCAGATGGCTGAGA
  TTGAATCAGATCTCCAAGTGACTCGACAGGAAAAGGAGGCGCTAAAGCAGGAAGTGATGTCATTACACAG
  ACAGCTTCAGAATGCCATTGATAAGGACTGGGTCTCGGAAACAGCTCCCCATCTCTCAGGGCTCCGGGGC
  CAGCAGAGAAGGCTGTCCTGGGACAAACTGGACCATCTGATGAATGAGGAACCACAGCTGCTTTGTCAAG
  AAAGCAAGAGACTCCAGACTGTGGTACAGAATACCCAAGCAGATCTCACCCACTCCCGGGAGAAGGTCCG
  TCAACTGGAATCCAACCTCCTTCCCACCAAACACCAAAAACAACTCAACCAACCATGTACTGTGAAGTCC
  ACAGAACAAGAAAAACTGACCTTAAAGAGAGAGTGTGAACAGTCTCAGAAAGAACAATCTCCTACCAGCA
  GGAAGGTGGGTCAGATGGGTTCCCTTGAGCGAGGATTAGAAACAATCCATTTGGAAAACGAAGGTCTGAA
  GAAGAAACAGATGCAGCCCCTGAGGTCCACTGTGACGCGTAGCCCATCCTCTCACTGGGATCTGCAGTTG
  CTCCAGCAGCAAGCCTGTCCGATGGTGCCCAGGGAGCAGTTTCTGCAGCTTCAACAGCAGCTGCTGCAGG
  CAGAAAAGAGAAGCCAGCACCTGCAGGAGGAGCTCGAAAACAGAACCTCCGAAACCAACACACCACAGGC
  TTTGCTACTGGAGCAGCGAGCTGTGCATGCAGATTCCTGCAGAAGGATTGGGCACCTC
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231905 representing NM_001286080
  Red=Cloning site Green=Tags(s)
  
  MDEVEEDQHEARLKELFDSFDTLGTGSLGQEELTDLCHVLCLEDVGPVLQQTLLQDNLLGRVHFDQFKEA
  LILILSRTLSSEEHFEESDCSPEAQPKYVRGGKRYGRRSLPEFQESGEEIEEVTVLEPLEEEARSSPIPA
  GDCGEHWKTQRSEEYEAEGQLRFWNPDDLNASHGGSCPPPDWIEEKLQEVCEDLGITRDGHLNRKKLVSI
  CEQYGLQNVDGAMLEEVFLSLDPDGTMSVEDFFYGLFKTGKSLTPSASTPYRQLKRHLSMQSFDESGRRT
  ATSSAMTSTIGFRVFSCLDDGMGQASVERILDTWQEEGIENSQEILKALDFSLDGNINLTELTLALENEL
  LVTKNGIHQAALASFKAEIRHLLERVDQVVREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLR
  KLDEEYKERIAALKNELRQEREQMLQQVGKQRVELEQEIQKAKTEENYIRDRLALSLKENNRLETELLEN
  AEKLAEYESLTQKLQRSLENVLAEKFGDLDPSSAEFFLQEERLAQMRNEYEQQCRLLQDQVDELQSELEE
  YQAQGRVLRLPLKNSLSEELDGHSGGIEPDQGPGSEECNPLNMSIEAELVIEQMKEQHHRDLCHLRLELE
  DKVRHYEKQLDDTRVASEQEQAAMKQKYEQGVHTLEKRVSELRSEIADLEGQAAVLREAHHKASCRHEEE
  KRQLQMAFDEEKAQLQEELRQEHERELQARLQQAAESFRQEREGLAQAAWTEEKVRGLEQSYQEQLLSLE
  EKHALEKEELREELSEHHRRELQEGREEMETECNRRVSQIEAQCQADCEKVTEHCEQTLQSLEVRHRQEL
  RDLLDQHLEERSQWEFEKDELTQECTDAQEQLKEALQRERATAAAMKQEQEILERTYKDRLNILSTERKQ
  LLQDLKDLQNASESQHGLLSGQILELKRSQERELRDQGQALCQTGVSEQLASQQLERLRVEHEQERREMT
  GKLAALESAHRASLERADQEKAEMSTEICRLQNTVKDMQQAASLLMLQGGCQATAGEEAEGDGAMSLLQQ
  GEQLLEENGDVLISLQRAHEHAVKENAKMATEISRLQQRLKKLEPGSVISSCLEEGTSEISGSSREQVEP
  IMKQGPATKHFLSDLGDHEARDLASTGTSSVQRQECKTEASEASLDCFSELENSEDTRTESWDLKSQISQ
  LREQLTVLRADCDRASERKQDLLFDISVLKKKLKMLERLPEASSRYKVLYEDAARENSCLQEELRLVETR
  YEESLDSNKELTAEVYRLQDEMKKMEEVMETFLSLEKSYDEVKVENEELRALVLRLQGKMEKVLGRAALQ
  GDSYALWEAPSENLEVASDEKMLELRQTPKECTPKVVSMHHIIEECTQETQCCEQGSTKLLARIKAHEIA
  WFHRAIKTHPEKPSAQNRVIPEGSAALLGLQDKHLQQEATIAELELEKQKLQELTRNLRERVTALVRQKD
  APSQGQKEEELKAMMQDLQITCGEMQRKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLEELNGS
  QEELWQKIETIEQEKASIQTMVEKLKKQVSDLKIKNQQLDSENIELSQKNSQNKEELKTLNQRLAEMLCQ
  REEPGACTSEKWEQENASLKEELDHYKVQTSTLVSSLEAELSEVKLQTHVMEQENLLLKDELERLKQLHR
  CPDLSDFQQKMSSILSYNEKLLKEKEVLSEELKSCADKLAESSLLEHRIATMKQEQTAWEEQSESLKSQL
  AVSQAKVQNLEDVLQNVNLQMAEIESDLQVTRQEKEALKQEVMSLHRQLQNAIDKDWVSETAPHLSGLRG
  QQRRLSWDKLDHLMNEEPQLLCQESKRLQTVVQNTQADLTHSREKVRQLESNLLPTKHQKQLNQPCTVKS
  TEQEKLTLKRECEQSQKEQSPTSRKVGQMGSLERGLETIHLENEGLKKKQMQPLRSTVTRSPSSHWDLQL
  LQQQACPMVPREQFLQLQQQLLQAEKRSQHLQEELENRTSETNTPQALLLEQRAVHADSCRRIGHL
  
  TRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-NotI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286080
• ORF Size: 6078 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286080.2, NP_001273009.1
• RefSeq Size: 6791 bp
• RefSeq ORF: 6081 bp
• Locus ID: 18080
• UniProt ID: Q61043
• Cytogenetics: 12 C2
• MW: 234.1 kDa
• Gene Summary: Centrosomal protein required for the positioning and anchorage of the microtubule minus-end in epithelial cells (PubMed:15784680, PubMed:10934040). May also act as a centrosome maturation factor (By similarity). May play a role in microtubule nucleation, by recruiting the gamma-tubulin ring complex to the centrosome (PubMed:15784680). Overexpression does not perturb nucleation or elongation of microtubules but suppresses release of microtubules (By similarity). Required for centriole organization and microtubule anchoring at the mother centriole (By similarity).[UniProtKB/Swiss-Prot Function]