Shroom3 (NM_001077595) Mouse Tagged ORF Clone

CAT#: MR231877

Shroom3 (myc-DDK-tagged) - Mouse shroom family member 3 (Shroom3), transcript variant 2

Product Images

Circular map for MR231877

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Shroom3
• Synonyms: AL022960; D5Ertd287e; Shrm; Shrm3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231877 representing NM_001077595
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCAGATATCTCAGGGCACAATGGGCCCTCCTTGGCACCAGAGCTACCATTCCAGCTCTTCGACCA
  GTGACCTCTCCAACTACGACCATGCTTATCTGAGGCGGAGCCCTGACCAGTGCAGCTCCCAGGGGAGCAT
  GGAAAGCCTGGAGCCCAGTGGCGGATACCCACCCTGCCATCTCCTTTCTCCTGCCAAGTCCACGAGCAGC
  ATCGACCAGCTAGGCCACCTTCATAACAAAAGGGACTCCGCATATAGCTCCTTCTCCACCAGTTCGAGCA
  TCTTCGAGTACCCACCCCCTGGTGGCTCTGCCCGAGAACGTTCGGGTTCCATGGACGTGATTTCTGCCCG
  GGGTGGCCTCCTAGAAGGGATGAGGCAGGCAGACATCCGCTACGTCAAGACGGTCTATGATACCCGGAGA
  GGAGTATCTTCAGAGTATGAGGTGAACCCTTCAGCCTTGCTCCTTCAAGGTAGGGATGCCCATGCCTCAG
  CCGACAGTCAGGGCTGTGCTAAATGGCACAGCATTCCTCGGGGCAAGGGAACGCCTTCCCCGTCTTGGAG
  CCAGCAGTGTTCTGGTTCCCTGGAGACTGCTACTGACAACCTGCCCCAGAAGGCAGGCGCACCCCTGCCC
  CCCACCCGCAGTGATAGCTATGCAGCCTTCCGCCATCGCGAGCGTCCCAGCTCCTGGTCCAGCCTTGATC
  AGAAAAGGTTCTGCCGGCCTCAGACAAACTCTTCAGGCTCCCAGAAAACCCCATTCGCTGAGGACCAGCT
  GCATACAGTCCCAGAGAGGAGTCCAGAGAACAGCCCCCCAGTGAAATCTAAGCATAATTATACCCAGAAG
  GCGCAGCCTGGCCAACCTCTGCTGCCCACTGGCATCTATCCGGTACCTTCTCCGGAGCCTCACTTTGCCC
  AGGTGCCCCAGCCCTCTGTGAGTAGTAATGGCACCGTCTACCCTGCGCTAGTCAAAGAGAGTGGATACAC
  AGCTGCTCAGGGAACCTGTAACAAGATGGCTACCTTAGATGAGAACGGGAATCAAAATGAAGCTAGCAGG
  CCTGGGTTTGCCTTCTGTCAGCCCCTAGAACACAACTCGGTGACTCCGGTGGAGAAAAGGCCAGAACCCA
  CGGCCAAATACATCTACAAAGTCCATTTTTCTTCAGTGCCTGAAAATGAAGACTCCTCCCTAAAGAGGCA
  CATCACACCTCCCCACGGCCATAGTCCATATCCCAGCGAGAGAAAGAACATCCACGGTGGCAGCAGAGCG
  TGTTCCAATCACCACAGTCTCTCGTCCCCCCAGGCCCAGGCCCTGCACGTGGGTGATGACAGGAGGCCTT
  CCAGGCTCTCTCAGCCCTGGGAGGGTGACTTTCAGGAAGATCACAATGCCAACCTCAGGCAGAAAGTGGA
  GAGGGAGGGCCAAGGCCAGGGCCTGTCGGGCAACTCTGGCAGGACCAGATCGGCCTTCTCATCTCTGCAG
  AATATTCCAGAGAGTCTGAGAAGACAAAGCAACGTGGAGCTTGGAGAAGCCCAGGAGGTTCACCCCGGGG
  GCAGGTCCAAGGTGGAGGACCCTGGAAGGAAAGCAGGGGCTTCAGACATCAGGGGCTACCTGGACAGATC
  TGTTTCCTATCCGAGGCCCGAGGGGAAAATGAACGCCGTAGACTCCGTCCACAGTGCAGACTCCAGGTAT
  GAGGAGTCCCCGGCCCCAGCGCTGCCACAGACATCCGGTGCGAGCCAGCGGCGGCTCAGCTCCAGCAGCT
  CCGCCGCCCCGCAGTACAGGAAGCCCCATTGCTCCGTGCTGGAGAAGGTTTCTAGAATAGAGGAACGAGA
  GCAGGGGCGCCACAGACCCCTGAGCGTGGGCAGCTCTGCATACGGCCCCGGCTACAGGCCTGGGAGGACC
  GGCCCGACCCCCAGTACTTCCAGCAGTGACCTCGACGATCCAAAAGCCGGCAGCGTCCATTTCTCGGAGT
  CCACTGAACACCTGCGCAATGGGGAGCAGAACCCCCCAAACGGGGAGGCTAAGCAGGAAGAGGCTTCCCG
  GCCCCAGTGCAGCCATTTGATCCGGAGGGCCCCTGCGGACGGCCGTGGACCTCCTGCCCGAGGCGGCGAG
  CCCTCAAGGCCGGAGGCCCGCCTGCTCCGCAGCCAGAGCACCTTTCAGCTCTACAGTGAGGCGGAGAGGG
  AAGCCTCGTGGTCAGAGGACAGGCCCGGTACCCCTGAGTCGCCCCTGCTCGATGCGCCTTTCAGCCGCGC
  CTACAGAAACAGCATCAAGGATGCCCAGTCCCGCGTCCTGGGGGCCACGTCGTTTCGTCGCCGAGACCTG
  GAACCGGGCACCCCCGCCACCTCGAGGCCCTGGCGCCCGCGACCCGCCTCCGCCCACGTGGGGATGCGGA
  GCCCCGAGGCCGCGGTGCCCTCGTCCTCCCCGCACACCCCTCGGGAGCGACACAGCGTGACCCCGGCCGC
  GCCCCAGGCTGCCCGCCGAGGCCCACGCCGGCGCCTCACTGTCGAGCAGAAGAAGCGCTCCTACTCGGAG
  CCCGAGAAGATGAACGAGGTAGGGGTCTCGGAGGAGGCCGAGCCCACGCCCTGCGGCCCGCCGAGGCCCG
  CGCAGCCGCGCTTCTCGGAGAGCACCGTGGCCGACCGGCGCCGCATCTTCGAGCGCGACGGCAAGGCCTG
  CTCCACGCTCAGCCTGTCGGGCCCCGAGCTCAAGCAGTTCCAGCAGAGCGCCCTGGCCGACTACATCCAG
  CGTAAGACGGGCAAGCGGCCCACAGGAGCCGCCTGCACACCGGAGGCCGGGCTGCGCGAGCGCGCACAGA
  GCGCCTACCTCCAGGCCGGCCCCGCGGCGCCCGATGGCCCCGGCCTGGCCTCTGCCTGTAGCCTGAGCTC
  CCTGCGAGAGCCCGAGGCGCTGCCCCGCAAGGAGCACACTCATCCGTCGGCCGCGGACGGCCCGCAGGCG
  CCCCGTGATCGCAGCAGCTCCTTCGCCAGCGGACGCCTAGTCGGGGAGCGACGTCGCTGGGACCCTCAGG
  TCCCCAGGCAGCTGCTCAGCGGAGCGAACTGCGAACCGAGGGGCGTCCAGAGGATGGACGGGGCCCCGGG
  CGGGCCGCCTTCCTGGGGCATGGTGGCCGGGAAGGCCGGGAAGTCCAAGTCAGCCGAGGATCTGCTGGAG
  CGCTCCGACACACTGGCGGTCCCTGTGCATGTGAGGTCCAGGTCGTCTCCCACCTCCGACAAGAAAGGTC
  AGGATGTGCTCCTGAGGGAAGGCAGCAACTTTGGTTTTGTAAAGGATCCATGCTGTTTGGCTGGCCCTGG
  ACCTAGGTCTCTCAGCTGCTCAGACAAAGGCCAGAATGAGCTGGCATTGCCCCTGCACCACCCTACGCCC
  TGCTGGAACGGCTCAGGCTGCAAAGCCACTGTGGCTTCCTCAGCTCCTCCCGAATCTTCTGGAGCCGCGG
  ATCATCTGAAGCAACGTAGAGCGCCTGGTCCTAGGCCACTCTCAGCAGGAATGCACGGCCACTTCCCAGA
  CGCCAGAGCTGCCTCACTCAGCTCCCCTCTGCCATCACCTGTCCCCTCCAGTTACCGGTCACAGCTTGCC
  ATGGATCAGCAGACTGGGCAACAGCCTCCCTCATCCCCTGCCTCTGCAGTGACCCAGCCTACCAGTCCAA
  GAAGCCCTGAGCTCAGCAGCCCAGCATATGGGCTAGGGGAGGGGATGTGGAAGAGGACATCCCTGCCTCA
  GCGACCCCCTCCCCCTTGGGTGAAATGGGCCCACGCGGTCAGAGAGGACGGCCTTGCTGAGGACACCTTG
  GCGCCTGAGTTTGCGAACCTGAAGCACTATAGAAACCAGCCGAGTCGTCCGAGTTCCTGCAGCACTTCTG
  ACCCAGACACTCCAGGAAGGATCTCGCTCCGAATATCGGAGTCAGCCCTTCAACCTTCCCCACCGCCCCG
  GGGGGACTATGATGACGAAGTGTTCATGAAGGACTTGCACCCCAAGGTTACTTCAAGCCCCACATTTGAA
  GCTCTTCCCCCACCCCCGCCACCTTCGCCACCGAGCGAGGAACCTCTAGTGAATGGCACAGATGACTTTC
  CTCCGCCTCCTCCTCCCCAAGCCCTGTGTGAGGTACTGCTGGATGGTGAGGCATCCACGGAAGCTGGCAG
  CGGCCCCTGCAGAATTCCCAGGGTGATGGTCACAAGGGAAGGGCACGTGCCTGGGGCAGCCCATTCCGAA
  GGTAGTCAGATAATGACTGCCACACCACCCCAAACCTCGGCCAAAGGTTCAGAAGCCGAGTCCAACACGC
  CTTCCAGCGCGAGCGCCCAGCCTCAGCTCAACGGTTCTCCTGGCAAGCAGCTCTGTCCCAGCCAGACCCG
  AAACCTCACTTATGAACCTGTCGAGAGAACTCAGGATCTAGGAAAGAAAACTCACGCCGAGCCTCAGAAG
  ACCTCAGAAGATATCCGGACAGAGGCTCTAGCCAAGGAAATTGTCCACCAAGACAAGTCTCTGGCAGACA
  TTTTAGATCCTGACTCCAGGATGAAGACAACGATGGACCTGATGGAGGGTTTATTCCCTGGAGACGCCAG
  TGTGCTGATGGATAGTGGCGCAAAGAGGAAAGCCTTAGACATCACCGCCAGGCGTGCGGGATGTGAAGCC
  AAGGCGAGCGACCACAAGGAGGCAGTGAGTGTGCTGGTCAACTGTCCTGCCTACTACAGTGTCTCTGCTG
  CCAAGGCGGAGCTGCTCAACAAAATCAAAGACATGCCGGAGGAGCTGCAGGAGGAGGAGGGGCAGGAGGA
  TGTCAATGAGAAAAAGGCTGAGCTTATCGGAAGCCTTACCCACAAGCTGGAGAGCCTCCAGGAAGCCAAG
  GGGAGCCTGCTCACGGACATCAAGCTGAACAATGCCCTGGGAGAGGAGGTGGAGGCTCTGATCAGTGAGC
  TCTGCAAACCCAACGAGTTTGACAAGTACAAGATGTTCATAGGGGACCTGGACAAGGTGGTCAACCTGCT
  GCTGTCCCTGTCTGGACGCCTGGCCCGCGTGGAGAACGTCCTCAGAGGCCTTGGTGAAGACGCCAGCAAA
  GAGGAAAGGAGTTCTCTGAACGAGAAAAGGAAGGTTCTGGCTGGCCAGCATGAGGATGCACGGGAGCTCA
  AGGAGAACCTGGACCGGAGGGAGCGCGTGGTGCTGGATATCCTGGCCAACTACCTGTCCGCCGAGCAGCT
  GCAGGACTACCAGCACTTCGTGAAGATGAAGTCCACACTCCTCATTGAGCAGCGGAAGCTGGATGACAAG
  ATAAAGCTGGGCCAGGAGCAGGTCAGGTGCCTGCTGGAGTCGCTTCCCTCGGACTTCAGGCCCAAGGCAG
  GGGCCATCTCCCTCCCTCCAGCCCTCACCGGCCATGGGACTCCTGGGGGGACATCTGTATTCGGTGGTGT
  TTTCCCAACGTTGACCTCTCCTCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231877 representing NM_001077595
  Red=Cloning site Green=Tags(s)
  
  MMQISQGTMGPPWHQSYHSSSSTSDLSNYDHAYLRRSPDQCSSQGSMESLEPSGGYPPCHLLSPAKSTSS
  IDQLGHLHNKRDSAYSSFSTSSSIFEYPPPGGSARERSGSMDVISARGGLLEGMRQADIRYVKTVYDTRR
  GVSSEYEVNPSALLLQGRDAHASADSQGCAKWHSIPRGKGTPSPSWSQQCSGSLETATDNLPQKAGAPLP
  PTRSDSYAAFRHRERPSSWSSLDQKRFCRPQTNSSGSQKTPFAEDQLHTVPERSPENSPPVKSKHNYTQK
  AQPGQPLLPTGIYPVPSPEPHFAQVPQPSVSSNGTVYPALVKESGYTAAQGTCNKMATLDENGNQNEASR
  PGFAFCQPLEHNSVTPVEKRPEPTAKYIYKVHFSSVPENEDSSLKRHITPPHGHSPYPSERKNIHGGSRA
  CSNHHSLSSPQAQALHVGDDRRPSRLSQPWEGDFQEDHNANLRQKVEREGQGQGLSGNSGRTRSAFSSLQ
  NIPESLRRQSNVELGEAQEVHPGGRSKVEDPGRKAGASDIRGYLDRSVSYPRPEGKMNAVDSVHSADSRY
  EESPAPALPQTSGASQRRLSSSSSAAPQYRKPHCSVLEKVSRIEEREQGRHRPLSVGSSAYGPGYRPGRT
  GPTPSTSSSDLDDPKAGSVHFSESTEHLRNGEQNPPNGEAKQEEASRPQCSHLIRRAPADGRGPPARGGE
  PSRPEARLLRSQSTFQLYSEAEREASWSEDRPGTPESPLLDAPFSRAYRNSIKDAQSRVLGATSFRRRDL
  EPGTPATSRPWRPRPASAHVGMRSPEAAVPSSSPHTPRERHSVTPAAPQAARRGPRRRLTVEQKKRSYSE
  PEKMNEVGVSEEAEPTPCGPPRPAQPRFSESTVADRRRIFERDGKACSTLSLSGPELKQFQQSALADYIQ
  RKTGKRPTGAACTPEAGLRERAQSAYLQAGPAAPDGPGLASACSLSSLREPEALPRKEHTHPSAADGPQA
  PRDRSSSFASGRLVGERRRWDPQVPRQLLSGANCEPRGVQRMDGAPGGPPSWGMVAGKAGKSKSAEDLLE
  RSDTLAVPVHVRSRSSPTSDKKGQDVLLREGSNFGFVKDPCCLAGPGPRSLSCSDKGQNELALPLHHPTP
  CWNGSGCKATVASSAPPESSGAADHLKQRRAPGPRPLSAGMHGHFPDARAASLSSPLPSPVPSSYRSQLA
  MDQQTGQQPPSSPASAVTQPTSPRSPELSSPAYGLGEGMWKRTSLPQRPPPPWVKWAHAVREDGLAEDTL
  APEFANLKHYRNQPSRPSSCSTSDPDTPGRISLRISESALQPSPPPRGDYDDEVFMKDLHPKVTSSPTFE
  ALPPPPPPSPPSEEPLVNGTDDFPPPPPPQALCEVLLDGEASTEAGSGPCRIPRVMVTREGHVPGAAHSE
  GSQIMTATPPQTSAKGSEAESNTPSSASAQPQLNGSPGKQLCPSQTRNLTYEPVERTQDLGKKTHAEPQK
  TSEDIRTEALAKEIVHQDKSLADILDPDSRMKTTMDLMEGLFPGDASVLMDSGAKRKALDITARRAGCEA
  KASDHKEAVSVLVNCPAYYSVSAAKAELLNKIKDMPEELQEEEGQEDVNEKKAELIGSLTHKLESLQEAK
  GSLLTDIKLNNALGEEVEALISELCKPNEFDKYKMFIGDLDKVVNLLLSLSGRLARVENVLRGLGEDASK
  EERSSLNEKRKVLAGQHEDARELKENLDRRERVVLDILANYLSAEQLQDYQHFVKMKSTLLIEQRKLDDK
  IKLGQEQVRCLLESLPSDFRPKAGAISLPPALTGHGTPGGTSVFGGVFPTLTSPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001077595
• ORF Size: 5415 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001077595.2, NP_001071063.1
• RefSeq Size: 6838 bp
• RefSeq ORF: 5418 bp
• Locus ID: 27428
• UniProt ID: Q9QXN0
• Cytogenetics: 5 47.29 cM
• MW: 195.4 kDa
• Gene Summary: Controls cell shape changes in the neuroepithelium during neural tube closure. Induces apical constriction in epithelial cells by promoting the apical accumulation of F-actin and myosin II, and probably by bundling stress fibers. Induces apicobasal cell elongation by redistributing gamma-tubulin and directing the assembly of robust apicobasal microtubule arrays.[UniProtKB/Swiss-Prot Function]