Ptprs (NM_001252456) Mouse Tagged ORF Clone

CAT#: MR231819

Ptprs (myc-DDK-tagged) - Mouse protein tyrosine phosphatase, receptor type, S (Ptprs), transcript variant 4

Product Images

Circular map for MR231819

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ptprs
• Synonyms: AL022616; PTP; PTP-NU3; PTPNU-3; PTPsigma; Ptpt9; R-PTP-S; RPTPsigma
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231819 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCCACCTGGAGTCCCAGCGTGGTGTCTGTGGTGGGTCCTGTGGGGCTCTTCCTCGTACTGCTGG
  CCAGAGGATGCTTGGCTGAAGAACCACCCAGGTTTATCAGAGAGCCCAAGGATCAGATTGGAGTGTCGGG
  AGGCGTGGCCTCCTTCGTGTGCCAGGCCACGGGTGATCCTAAGCCACGGGTGACCTGGAACAAGAAGGGC
  AAGAAAGTGAACTCACAGCGCTTCGAGACCATTGACTTTGACGAGAGCTCTGGGGCGGTCCTGAGGATCC
  AGCCACTTCGGACGCCTCGGGATGAGAACGTGTACGAGTGTGTGGCCCAGAACTCGGTGGGCGAAATCAC
  AATTCATGCAAAGCTCACCGTCCTTCGAGAGGACCAGCTGCCTCCTGGCTTCCCCAACATTGACATGGGC
  CCCCAGTTGAAGGTTGTAGAGCGCACACGCACAGCCACCATGCTCTGTGCTGCCAGCGGGAACCCGGACC
  CTGAGATCACCTGGTTTAAGGACTTCCTGCCTGTGGACCCCAGTGCCAGCAACGGGCGGATCAAGCAGCT
  TCGATCAGGTGCCCTGCAGATTGAGAGCAGCGAGGAGACAGACCAGGGCAAGTACGAGTGTGTGGCCACC
  AACAGCGCTGGGGTGCGCTACTCATCACCTGCCAACCTCTACGTGCGAGTCCGCCGTGTGGCCCCACGCT
  TCTCCATCCTGCCCATGAGCCACGAGATCATGCCCGGTGGGAATGTGAATATCACTTGTGTGGCCGTGGG
  CTCACCCATGCCCTACGTGAAATGGATGCAGGGGGCCGAGGACCTGACGCCTGAGGATGACATGCCCGTG
  GGTCGGAATGTTCTAGAACTCACGGATGTCAAGGACTCAGCTAACTACACTTGTGTGGCCATGTCCAGCC
  TGGGTGTGATCGAGGCCGTGGCCCAGATCACTGTAAAATCTCTCCCCAAAGCCCCTGGGACTCCTGTGGT
  GACGGAGAACACTGCCACCAGTATCACTGTCACATGGGACTCGGGCAACCCTGACCCCGTGTCCTACTAC
  GTAATTGAGTATAAGTCCAAAAGCCAGGATGGGCCGTATCAGATCAAAGAAGACATCACCACCACGCGCT
  ACAGCATCGGAGGCCTGAGCCCCAATTCTGAGTATGAGATCTGGGTGTCAGCTGTCAACTCCATTGGCCA
  GGGCCCTCCCAGTGAATCGGTGGTGACCCGCACAGGTGAGCAGGCACCAGCCAGCGCTCCCAGGAATGTT
  CAGGCCCGCATGCTCAGCGCCACCACCATGATCGTGCAGTGGGAGGAGCCTGTGGAGCCCAATGGCCTGA
  TCCGTGGCTACCGTGTCTACTATACCATGGAGCCGGAACACCCAGTGGGCAACTGGCAGAAACACAATGT
  GGACGACAGTCTCCTGACCACTGTGGGCAGCCTGCTGGAAGACGAGACCTACACCGTGCGCGTGCTCGCC
  TTCACGTCGGTGGGCGACGGACCACTGTCAGACCCCATCCAGGTCAAGACCCAGCAGGGAGTTCCTGGCC
  AGCCCATGAACTTGCGGGCTGAGGCCAAGTCAGAGACCAGCATTGGGCTCTCGTGGAGTGCACCACGACA
  GGAGAGTGTCATTAAGTATGAACTGCTCTTCCGGGAGGGCGACCGAGGCCGAGAGGTGGGGCGAACCTTC
  GACCCAACCACAGCCTTTGTGGTGGAGGACCTCAAGCCCAATACGGAGTATGCGTTCCGGCTGGCGGCGC
  GCTCGCCGCAGGGCCTGGGCGCCTTCACCGCGGTCGTGCGCCAGCGCACGCTGCAGGCCATCTCCCCAAA
  GAACTTCAAGGTGAAGATGATCATGAAGACTTCAGTGCTGCTGAGCTGGGAGTTCCCCGACAACTATAAC
  TCACCCACACCCTACAAGATTCAGTACAATGGGCTCACCCTGGATGTGGACGGCCGCACGACCAAGAAGC
  TGATCACACACCTCAAGCCACACACCTTCTATAATTTCGTGCTCACCAACCGTGGCAGCAGCCTGGGGGG
  CCTGCAGCAGACGGTCACTGCCAGGACCGCCTTTAACATGCTCAGTGGCAAGCCTAGCGTCGCCCCGAAG
  CCCGACAATGACGGTTTCATCGTGGTCTACCTGCCTGATGGCCAGAGTCCTGTGACCGTGCAGAACTACT
  TCATTGTGATGGTCCCACTTCGGAAGTCTCGAGGTGGCCAGTTCCCTGTCCTACTAGGTAGTCCAGAGGA
  CATGGATCTGGAGGAGCTCATCCAGGACATCTCCCGGCTGCAGAGGCGCAGCCTGCGCCACTCCAGACAG
  CTGGAGGTGCCTCGGCCCTACATCGCCGCTCGATTCTCCATCCTGCCAGCTGTCTTCCATCCTGGGAACC
  AGAAGCAATATGGTGGCTTTGACAACAGGGGCTTGGAGCCAGGCCACCGCTATGTCCTCTTTGTGCTTGC
  TGTGTTGCAGAAGAATGAGCCTACATTTGCAGCCAGTCCCTTCTCAGACCCCTTCCAGCTGGACAACCCG
  GACCCTCAGCCCATTGTGGACGGCGAGGAGGGCCTCATCTGGGTGATTGGGCCTGTGCTGGCCGTGGTCT
  TCATCATCTGCATCGTGATTGCCATCCTGCTGTACAAGAACAAACCTGACAGCAAACGCAAGGACTCAGA
  GCCCCGCACCAAATGCTTACTGAACAATGCCGACCTTGCCCCCCATCACCCCAAGGACCCTGTGGAAATG
  CGACGCATCAACTTCCAGACACCAGGTATGCTCAGCCACCCACCCATCCCCATCACAGACATGGCGGAGC
  ACATGGAGAGACTCAAAGCCAACGACAGCCTGAAGCTCTCCCAGGAGTACGAGTCCATTGACCCCGGGCA
  GCAATTCACGTGGGAACATTCGAACCTGGAGGCCAACAAGCCCAAGAACCGCTATGCCAACGTCATCGCC
  TATGACCACTCACGAGTCATCCTGCAGCCCCTAGAAGGCATCATGGGTAGTGATTACATCAATGCCAACT
  ATGTGGACGGCTACCGGCGGCAGAATGCATACATTGCCACGCAGGGGCCCCTGCCTGAGACCTTTGGGGA
  CTTCTGGCGGATGGTGTGGGAGCAGCGATCGGCCACTGTGGTCATGATGACGCGACTGGAGGAGAAATCA
  CGGATCAAATGTGACCAATACTGGCCTAACCGAGGCACCGAGACATACGGCTTCATCCAGGTCACCCTAC
  TAGATACCATGGAGCTGGCTACCTTCTGCGTCAGGACTTTTTCTCTACACAAGAATGGCTCTAGCGAGAA
  GCGTGAGGTGCGACATTTCCAGTTCACGGCATGGCCCGACCACGGGGTACCTGAGTACCCCACGCCCTTC
  CTGGCATTCCTGCGAAGAGTCAAGACCTGCAACCCGCCTGATGCTGGCCCCATTGTGGTCCACTGCAGCG
  CGGGTGTGGGGCGCACTGGCTGCTTCATCGTAATTGACGCCATGCTAGAGCGCATCAAGACAGAGAAGAC
  CGTGGATGTGTATGGACATGTGACACTCATGCGGTCGCAGCGCAACTACATGGTGCAGACAGAGGATCAG
  TATGGCTTCATCCACGAGGCGCTGCTGGAGGCTGTGGGCTGCGGCAATACCGAGGTCCCTGCTCGCAGCC
  TCTACACCTACATCCAGAAGCTGGCCCAGGTGGAGCCTGGCGAGCACGTCACGGGCATGGAGCTTGAGTT
  CAAGAGGCTCGCCAGTTCCAAGGCACACACTTCGCGCTTCATCACCGCCAGCCTGCCTTGCAACAAGTTT
  AAGAACCGACTGGTGAACATCCTGCCGTACGAGAGCTCGCGTGTCTGCCTGCAGCCCATCCGCGGTGTGG
  AGGGCTCTGACTACATCAATGCCAGCTTTATCGACGGCTATAGACAGCAGAAAGCCTACATTGCAACACA
  GGGGCCACTGGCAGAGACCACAGAGGACTTCTGGCGAGCTCTGTGGGAGAACAACTCTACTATTGTCGTA
  ATGCTCACCAAGCTCCGAGAAATGGGCCGGGAAAAGTGCCACCAGTACTGGCCAGCCGAGCGCTCTGCCC
  GCTACCAGTACTTTGTGGTTGACCCGATGGCAGAGTATAACATGCCACAGTACATTCTGCGTGAGTTTAA
  GGTCACAGATGCCCGGGATGGCCAGTCCCGGACCGTCCGACAGTTCCAGTTCACGGACTGGCCAGAGCAG
  GGTGCACCCAAGTCAGGGGAAGGCTTCATTGACTTCATCGGCCAAGTGCATAAGACCAAGGAGCAGTTTG
  GCCAGGACGGACCCATCTCAGTGCACTGCAGCGCCGGAGTGGGCAGGACCGGAGTGTTCATCACCCTGAG
  CATCGTGCTTGAGCGGATGCGCTACGAGGGCGTGGTGGACATTTTCCAGACAGTGAAGGTGCTTCGGACC
  CAGAGGCCTGCCATGGTGCAGACAGAGGACGAGTACCAGTTCTGCTTCCAGGCGGCTTTGGAATACCTGG
  GCAGTTTTGATCATTATGCAACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231819 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAPTWSPSVVSVVGPVGLFLVLLARGCLAEEPPRFIREPKDQIGVSGGVASFVCQATGDPKPRVTWNKKG
  KKVNSQRFETIDFDESSGAVLRIQPLRTPRDENVYECVAQNSVGEITIHAKLTVLREDQLPPGFPNIDMG
  PQLKVVERTRTATMLCAASGNPDPEITWFKDFLPVDPSASNGRIKQLRSGALQIESSEETDQGKYECVAT
  NSAGVRYSSPANLYVRVRRVAPRFSILPMSHEIMPGGNVNITCVAVGSPMPYVKWMQGAEDLTPEDDMPV
  GRNVLELTDVKDSANYTCVAMSSLGVIEAVAQITVKSLPKAPGTPVVTENTATSITVTWDSGNPDPVSYY
  VIEYKSKSQDGPYQIKEDITTTRYSIGGLSPNSEYEIWVSAVNSIGQGPPSESVVTRTGEQAPASAPRNV
  QARMLSATTMIVQWEEPVEPNGLIRGYRVYYTMEPEHPVGNWQKHNVDDSLLTTVGSLLEDETYTVRVLA
  FTSVGDGPLSDPIQVKTQQGVPGQPMNLRAEAKSETSIGLSWSAPRQESVIKYELLFREGDRGREVGRTF
  DPTTAFVVEDLKPNTEYAFRLAARSPQGLGAFTAVVRQRTLQAISPKNFKVKMIMKTSVLLSWEFPDNYN
  SPTPYKIQYNGLTLDVDGRTTKKLITHLKPHTFYNFVLTNRGSSLGGLQQTVTARTAFNMLSGKPSVAPK
  PDNDGFIVVYLPDGQSPVTVQNYFIVMVPLRKSRGGQFPVLLGSPEDMDLEELIQDISRLQRRSLRHSRQ
  LEVPRPYIAARFSILPAVFHPGNQKQYGGFDNRGLEPGHRYVLFVLAVLQKNEPTFAASPFSDPFQLDNP
  DPQPIVDGEEGLIWVIGPVLAVVFIICIVIAILLYKNKPDSKRKDSEPRTKCLLNNADLAPHHPKDPVEM
  RRINFQTPGMLSHPPIPITDMAEHMERLKANDSLKLSQEYESIDPGQQFTWEHSNLEANKPKNRYANVIA
  YDHSRVILQPLEGIMGSDYINANYVDGYRRQNAYIATQGPLPETFGDFWRMVWEQRSATVVMMTRLEEKS
  RIKCDQYWPNRGTETYGFIQVTLLDTMELATFCVRTFSLHKNGSSEKREVRHFQFTAWPDHGVPEYPTPF
  LAFLRRVKTCNPPDAGPIVVHCSAGVGRTGCFIVIDAMLERIKTEKTVDVYGHVTLMRSQRNYMVQTEDQ
  YGFIHEALLEAVGCGNTEVPARSLYTYIQKLAQVEPGEHVTGMELEFKRLASSKAHTSRFITASLPCNKF
  KNRLVNILPYESSRVCLQPIRGVEGSDYINASFIDGYRQQKAYIATQGPLAETTEDFWRALWENNSTIVV
  MLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTVRQFQFTDWPEQ
  GAPKSGEGFIDFIGQVHKTKEQFGQDGPISVHCSAGVGRTGVFITLSIVLERMRYEGVVDIFQTVKVLRT
  QRPAMVQTEDEYQFCFQAALEYLGSFDHYAT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001252456
• ORF Size: 4503 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252456.1, NP_001239385.1
• RefSeq Size: 5618 bp
• RefSeq ORF: 4506 bp
• Locus ID: 19280
• UniProt ID: B0V2N1
• Cytogenetics: 17 29.32 cM
• MW: 168.3 kDa
• Gene Summary: Cell surface receptor that binds to glycosaminoglycans, including chondroitin sulfate proteoglycans and heparan sulfate proteoglycans (PubMed:19833921, PubMed:21454754, PubMed:22406547). Binding to chondroitin sulfate and heparan sulfate proteoglycans has opposite effects on PTPRS oligomerization and regulation of neurite outgrowth (PubMed:21454754). Contributes to the inhibition of neurite and axonal outgrowth by chondroitin sulfate proteoglycans, also after nerve transection (PubMed:15797710, PubMed:19833921, PubMed:19780196, PubMed:21454754, PubMed:22519304, PubMed:22406547). Plays a role in stimulating neurite outgrowth in response to the heparan sulfate proteoglycan GPC2 (PubMed:21454754). Required for normal brain development, especially for normal development of the pituitary gland and the olfactory bulb (PubMed:10080191). Functions as tyrosine phosphatase (PubMed:7529177). Mediates dephosphorylation of NTRK1, NTRK2 and NTRK3 (By similarity). Plays a role in down-regulation of signaling cascades that lead to the activation of Akt and MAP kinases (PubMed:15797710). Down-regulates TLR9-mediated activation of NF-kappa-B, as well as production of TNF, interferon alpha and interferon beta (PubMed:26231120).[UniProtKB/Swiss-Prot Function]