Stag2 (NM_021465) Mouse Tagged ORF Clone

CAT#: MR231702

Stag2 (myc-DDK-tagged) - Mouse stromal antigen 2 (Stag2), transcript variant 1

Product Images

Circular map for MR231702

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Stag2
• Synonyms: 9230105L23Rik; B230112I07Rik; SA-2; SAP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231702 representing NM_021465
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATAGCAGCTCCAGAAATACCAACTGATTTTAATCTACTGCAGGAGTCAGAGACACACTTTTCTTCTG
  ACACAGATTTTGAAGACATTGAAGGAAAAAATCAGAAGCAAGGCAAAGGCAAAACTTGTAAAAAAGGCAA
  AAAGGGTCCAGCAGAAAAGGGTAAAAGTGGAAATGGAGGAGGAAAACCTCCTTCTGGCTCAAACCGAATG
  AACGGTCATCATCAACAGAATGGAGTTGAAAACATGATGTTGTTCGAAGTTGTTAAAATGGGCAAGAGTG
  CTATGCAGTCGGTGGTAGATGACTGGATCGAGTCCTACAAGCATGACCGGGACATAGCGCTTCTTGACCT
  TATCAATTTCTTTATTCAGTGCTCAGGCTGCAAAGGAGTCGTCACAGCAGAGATGTTCAGACATATGCAG
  AACTCTGAGATAATTCGAAAAATGACAGAAGAATTTGACGAGGATAGTGGGGATTACCCACTTACCATGG
  CTGGTCCTCAGTGGAAGAAGTTCAAGTCCAGCTTCTGTGAGTTCATTGGTGTGTTAGTACGGCAGTGTCA
  ATACAGTATCATATATGATGAGTACATGATGGATACTGTCATTTCACTTCTTACTGGATTGTCTGACTCC
  CAAGTCAGAGCATTTCGACATACAAGCACCCTGGCAGCTATGAAGTTGATGACTGCTTTGGTAAATGTGG
  CACTAAATCTTAGCATTAATATGGATAACACACAAAGACAGTACGAGGCAGAACGGAATAAAATGATTGG
  AAAACGAGCTAACGAAAGGCTAGAACTCCTGTTACAAAAGCGTAAAGAGCTTCAGGAAAATCAAGATGAG
  ATAGAAAATATGATGAATGCAATATTTAAAGGAGTGTTTGTACATAGATACAGGGATGCAATAGCTGAAA
  TCAGAGCTATATGCATTGAAGAGATTGGCATTTGGATGAAGATGTATAGTGATGCCTTCCTTAATGACAG
  CTATTTAAAATATGTTGGTTGGACTATGCATGATAAGCAAGGTGAAGTCAGACTCAAATGTCTTACTGCT
  TTACAAGGGCTTTACTATAACAAAGAACTTAATTCCAAACTGGAACTCTTTACCAGTCGATTCAAGGATA
  GAATTGTGTCTATGACCCTTGACAAAGAATATGATGTTGCAGTACAAGCAATAAAGTTACTCACTCTTGT
  TTTACAGAGTAGTGAAGAAGTTCTAACTGCAGAAGATTGTGAAAATGTCTATCATCTGGTTTATTCAGCT
  CACCGGCCAGTAGCAGTTGCAGCTGGAGAATTTCTCTACAAAAAGCTCTTCAGCCGCAGAGACCCAGAAG
  AGGATGGGCTGATGAAAAGAAGGGGAAGGCAAGGCCCGAATGCCAACCTTGTGAAGACTTTGGTGTTTTT
  CTTTTTGGAAAGTGAGTTACACGAGCATGCAGCTTACCTTGTGGACAGCATGTGGGATTGTGCTACTGAG
  CTCCTGAAAGACTGGGAATGTATGAATAGCTTGTTATTAGAAGAGCCTCTTAGTGGAGAGGAAGCTCTAA
  CAGACAGACAAGAGAGTGCTTTGATTGAAATAATGCTTTGTACTATTAGACAAGCAGCTGAATGTCATCC
  TCCCGTGGGAAGAGGGACAGGAAAAAGGGTGCTGACAGCAAAGGAGAAGAAGACACAGTTGGATGACAGA
  ACAAGAATCACTGAGCTGTTTGCTGTGGCCCTTCCGCAGTTATTAGCTAAATACTCTGTAGATGCAGAAA
  AGGTGACCAATTTGTTGCAGTTACCTCAGTACTTTGATTTGGAGATATACACCACTGGACGATTAGAAAA
  GCATTTGGATGCCTTATTGCGCCAGATCCGAAATATTGTAGAGAAGCACACAGACACAGATGTTTTGGAA
  GCATGTTCTAAAACTTACCATGCATTATGTAACGAAGAGTTCACCATCTTCAACAGAGTAGATATTTCAA
  GAAGTCAGCTGATTGATGAACTGGCAGATAAATTTAACCGGCTTCTTGAAGATTTTCTGCAAGAGGGAGA
  AGAACCTGATGAAGATGACGCATATCAGGTATTGTCAACATTGAAGAGGATCACTGCTTTTCATAATGCT
  CATGATCTTTCAAAGTGGGATTTGTTCGCTTGTAATTACAAACTCTTGAAAACCGGAATCGAAAATGGAG
  ATATGCCTGAGCAGATTGTTATTCACGCACTGCAGTGTGCTCACTATGTAATCCTTTGGCAGCTTGCTAA
  GATCACTGAAAGCACGTCTACAAAGGAGGACTTACTGCGCTTAAAGAAACAGATGAGGGTATTTTGCCAG
  ATATGTCAGCATTACTTGACCAACGTGAATACTACTGTTAAAGAACAGGCCTTCACTATTCTGTGTGACA
  TTTTGATGATCTTCAGTCACCAGATTATGTCTGGAGGGCGTGACATGTTAGAGCCATTAGTTTATACTCC
  TGATTCTTCATTGCAGTCTGAGTTGCTCAGCTTTATTTTGGATCATGTCTTCATTGAACAGGATGATGAT
  AGTAATAGTGCAGATGGTCAGCAAGAGGATGAAGCCAGCAAAATTGAAGCTTTGCACAAGAGGAGAAATT
  TACTTGCAGCATTTTGTAAGCTAATTGTATATACCGTGGTGGAGATGAACACAGCTGCAGATATCTTCAA
  GCAGTATATGAAGTATTATAATGATTATGGCGATATCATCAAAGAAACAATGAGTAAAACAAGGCAGATT
  GACAAAATTCAGTGTGCTAAGACCCTTATTCTCAGTTTGCAGCAGCTTTTTAATGAGATGATACAAGAGA
  ATGGCTATAATTTTGATAGATCATCCTCAACATTTAGTGGCATAAAAGAACTTGCTCGGCGTTTTGCTTT
  AACTTTTGGACTTGATCAATTGAAAACCAGAGAAGCCATTGCCATGCTCCACAAAGATGGTATAGAATTT
  GCTTTTAAGGAGCCTAATCCACAAGGGGAGAGCCATCCACCTTTAAACTTGGCATTTCTTGATATTCTCA
  GTGAATTTTCTTCTAAACTGCTCCGACAGGATAAAAGAACAGTGTATGTTTATTTGGAAAAGTTCATGAC
  CTTTCAGATGTCACTCCGAAGAGAAGATGTATGGCTTCCTCTGATGTCTTACCGAAATTCTTTGCTAGCT
  GGTGGTGATGATGATACTATGTCAGTCATTAGTGGAATGAGCAGTCGGGGATCAACTGTACGGAGTAAAA
  AATCAAAACCATCTACAGGAAAGCGGAAAGTGGTTGAAGGCATGCAACTAGCACTCCCTGAAGAAAGCAG
  TAGCAGTGACAGTATGTGGTTGAGCAGAGAGCAAACACTGCACACCCCTGTCATGATGCAGACACCACAG
  CTCACCTCCACTATTATGAGAGAACCGAAAAGGTTACGGCCTGAAGATAGCTTCATGAGTGTCTATCCGA
  TGCAGGCAGAACACCATCAAACGCCTCTGGATTATAATCGGCGTGGTACAAGCCTAATGGAAGATGATGA
  AGAGCCAATTGTTGAAGATGTTATGATGTCCTCAGAAGGAAGGATTGAGGATCTTAATGAGGGAATGGAC
  TTTGACACCATGGATATAGACTTGCCACCATCAAAGAATAGAAGAGAGAGAACAGAACTGAAGCCCGATT
  TCTTTGATCCAGCTTCAATTATGGATGAATCAGTTCTTGGGGTGTCAATGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231702 representing NM_021465
  Red=Cloning site Green=Tags(s)
  
  MIAAPEIPTDFNLLQESETHFSSDTDFEDIEGKNQKQGKGKTCKKGKKGPAEKGKSGNGGGKPPSGSNRM
  NGHHQQNGVENMMLFEVVKMGKSAMQSVVDDWIESYKHDRDIALLDLINFFIQCSGCKGVVTAEMFRHMQ
  NSEIIRKMTEEFDEDSGDYPLTMAGPQWKKFKSSFCEFIGVLVRQCQYSIIYDEYMMDTVISLLTGLSDS
  QVRAFRHTSTLAAMKLMTALVNVALNLSINMDNTQRQYEAERNKMIGKRANERLELLLQKRKELQENQDE
  IENMMNAIFKGVFVHRYRDAIAEIRAICIEEIGIWMKMYSDAFLNDSYLKYVGWTMHDKQGEVRLKCLTA
  LQGLYYNKELNSKLELFTSRFKDRIVSMTLDKEYDVAVQAIKLLTLVLQSSEEVLTAEDCENVYHLVYSA
  HRPVAVAAGEFLYKKLFSRRDPEEDGLMKRRGRQGPNANLVKTLVFFFLESELHEHAAYLVDSMWDCATE
  LLKDWECMNSLLLEEPLSGEEALTDRQESALIEIMLCTIRQAAECHPPVGRGTGKRVLTAKEKKTQLDDR
  TRITELFAVALPQLLAKYSVDAEKVTNLLQLPQYFDLEIYTTGRLEKHLDALLRQIRNIVEKHTDTDVLE
  ACSKTYHALCNEEFTIFNRVDISRSQLIDELADKFNRLLEDFLQEGEEPDEDDAYQVLSTLKRITAFHNA
  HDLSKWDLFACNYKLLKTGIENGDMPEQIVIHALQCAHYVILWQLAKITESTSTKEDLLRLKKQMRVFCQ
  ICQHYLTNVNTTVKEQAFTILCDILMIFSHQIMSGGRDMLEPLVYTPDSSLQSELLSFILDHVFIEQDDD
  SNSADGQQEDEASKIEALHKRRNLLAAFCKLIVYTVVEMNTAADIFKQYMKYYNDYGDIIKETMSKTRQI
  DKIQCAKTLILSLQQLFNEMIQENGYNFDRSSSTFSGIKELARRFALTFGLDQLKTREAIAMLHKDGIEF
  AFKEPNPQGESHPPLNLAFLDILSEFSSKLLRQDKRTVYVYLEKFMTFQMSLRREDVWLPLMSYRNSLLA
  GGDDDTMSVISGMSSRGSTVRSKKSKPSTGKRKVVEGMQLALPEESSSSDSMWLSREQTLHTPVMMQTPQ
  LTSTIMREPKRLRPEDSFMSVYPMQAEHHQTPLDYNRRGTSLMEDDEEPIVEDVMMSSEGRIEDLNEGMD
  FDTMDIDLPPSKNRRERTELKPDFFDPASIMDESVLGVSMF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021465
• ORF Size: 3693 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021465.4, NP_067440.3
• RefSeq Size: 5837 bp
• RefSeq ORF: 3696 bp
• Locus ID: 20843
• UniProt ID: O35638
• Cytogenetics: X A4
• MW: 141.7 kDa
• Gene Summary: Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis (By similarity).[UniProtKB/Swiss-Prot Function]