Ehmt2 (NM_001286575) Mouse Tagged ORF Clone

CAT#: MR231680

Ehmt2 (myc-DDK-tagged) - Mouse euchromatic histone lysine N-methyltransferase 2 (Ehmt2), transcript variant 4

Product Images

Circular map for MR231680

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ehmt2
• Synonyms: Bat8; D17Ertd710e; G9a; KMT1C; NG36
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231680 representing NM_001286575
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCGGCGGGAGCTGCTGCGGCGGCGGCCGCCGAGGGGGAGGCCCCCGCTGAGATGGGGGCGC
  TGCTGCTGGAGAAGGAGCCCCGAGGAGCCGCCGAGAGAGTTCATAGCTCTTTGGGGGACACCCCTCAGAG
  TGAGGAGACCCTTCCCAAGGCCAACCCCGACTCCTTGGAGCCTGCCGGCCCCTCCTCTCCGGCCTCTGTC
  ACTGTCACCGTCGGCGATGAGGGGGCTGACACCCCTGTCGGGGCCGCATCACTCATCGGGGACGAACCCG
  AGAGCCTGGAGGGAGATGGGGGTCGCATCGTGCTGGGCCATGCCACAAAGTCGTTCCCCTCTTCCCCCAG
  CAAGGGGGGTGCCTGTCCCAGTCGGGCCAAAATGTCAATGACAGGGGCAGGAAAGTCGCCCCCCTCGGTC
  CAGAGTTTGGCCATGAGGCTGTTGAGCATGCCCGGGGCCCAGGGAGCTGCAACTGCTGGGCCTGAACCCT
  CTCCGGCAACAACTGCCGCCCAGGAGGGGCAGCCCAAAGTGCACCGAGCCCGGAAAACCATGTCCAAACC
  TAGCAACGGACAGCCTCCAATCCCTGAGAAGCGGCCCCCTGAAGTCCAGCATTTCCGCATGAGTGATGAC
  ATGCATCTGGGGAAGGTGACTTCAGATGTGGCCAAAAGGAGGAAGCTGAACTCTGGTAGCCTGTCCGAGG
  ACTTGGGCTCTGCCGGGGGCTCAGGAGATATAATCCTGGAGAAGGGAGAGCCCAGGCCCCTGGAGGAGTG
  GGAGACGGTGGTGGGCGATGACTTCAGCCTGTACTATGATGCGTACTCTGTGGATGAGCGGGTGGACTCT
  GACAGCAAGTCTGAAGTCGAAGCTCTAGCTGAACAGTTGAGTGAGGAGGAGGAGGAGGAAGAGGAGGAAG
  AAGAAGAAGAGGAGGAGGAGGAGGAAGAGGAGGAGGAGGAAGAAGAGGACGAGGAGTCGGGCAATCAGTC
  AGACAGGAGCGGTTCTAGTGGCCGGCGCAAGGCCAAGAAGAAATGGCGGAAAGACAGCCCGTGGGTGAAG
  CCATCTAGAAAACGGCGGAAACGAGAGCCTCCGAGGGCCAAGGAGCCAAGAGGAGTGAATGGTGTGGGTT
  CCTCAGGGCCCAGTGAGTACATGGAGGTTCCTCTGGGGTCCCTGGAGCTGCCCAGCGAGGGGACCCTCTC
  CCCCAACCACGCTGGGGTCTCCAATGACACGTCTTCACTGGAGACAGAACGCGGGTTTGAGGAGCTGCCC
  CTCTGCAGCTGCCGCATGGAGGCTCCCAAGATTGACCGCATCAGCGAGAGAGCAGGGCACAAGTGCATGG
  CCACAGAGAGTGTGGATGGAGAGCTCCTGGGCTGCAATGCTGCCATCCTTAAGCGGGAGACCATGCGGCC
  GTCTAGCCGCGTGGCGCTGATGGTGCTCTGTGAGGCCCATCGAGCCCGCATGGTCAAGCACCATTGCTGC
  CCGGGCTGCGGCTACTTCTGCACAGCGGGCACCTTCCTGGAATGCCACCCCGACTTTCGTGTAGCTCACC
  GCTTCCATAAGGCCTGCGTATCCCAGCTCAATGGGATGGTCTTCTGTCCCCACTGTGGAGAGGATGCCTC
  AGAGGCCCAGGAGGTGACCATTCCTCGGGGCGATGGGGGAACACCCCCAATTGGCACCGCAGCTCCTGCT
  CTGCCACCCCTGGCACATGATGCCCCAGGGCGAGCGGATACCTCCCAGCCTAGCGCCCGAATGCGAGGGC
  ATGGAGAGCCGCGGCGCCCGCCCTGTGATCCCCTGGCTGACACCATCGACAGCTCAGGGCCTTCACTGAC
  TCTGCCTAATGGGGGCTGCCTCTCCGCTGTGGGTCTGCCCCCAGGGCCGGGCAGGGAAGCCCTGGAAAAA
  GCCTTGGTCATCCAGGAGTCTGAGAGGCGGAAGAAGCTGCGATTCCACCCACGGCAGCTGTACCTGTCGG
  TGAAGCAGGGGGAGCTGCAGAAGGTGATCCTTATGCTGTTAGACAACCTGGACCCCAACTTCCAGAGCGA
  CCAGCAGAGCAAGCGCACGCCCCTGCACGCGGCCGCCCAGAAGGGGTCGGTAGAGATCTGTCATGTGCTG
  CTGCAGGCAGGAGCCAACATCAATGCCGTAGATAAGCAACAACGCACGCCACTAATGGAGGCCGTGGTGA
  ACAACCACCTGGAGGTGGCACGCTACATGGTGCAGTTAGGTGGCTGTGTCTACAGCAAGGAAGAGGATGG
  CTCCACCTGTCTACATCATGCAGCCAAAATTGGGAACTTGGAAATGGTCAGCCTGCTACTGAGCACAGGA
  CAGGTGGACGTCAATGCCCAGGACAGTGGGGGCTGGACGCCCATCATCTGGGCAGCCGAGCACAAGCACA
  TCGATGTGATTCGTATGCTGCTGACCCGGGGTGCCGATGTCACCCTGACTGACAATGAGGAAAACATCTG
  CCTGCACTGGGCCTCCTTCACGGGTAGTGCCGCCATCGCTGAGGTCCTTCTGAATGCCCAGTGTGATCTC
  CATGCTGTCAACTACCATGGGGACACGCCCCTGCACATAGCCGCCAGGGAGAGCTACCATGACTGTGTTC
  TGTTGTTCCTGTCTCGTGGAGCCAACCCTGAGCTTCGGAACAAAGAAGGAGACACGGCATGGGATCTGAC
  CCCAGAGCGCTCTGATGTGTGGTTTGCACTGCAGCTCAATCGAAAGCTTAGGCTTGGGGTAGGGAACCGG
  GCTGTCCGCACCGAGAAGATCATCTGCCGGGACGTAGCCCGAGGCTATGAGAATGTACCCATCCCCTGTG
  TCAATGGTGTGGATGGGGAGCCGTGCCCGGAGGACTACAAGTACATCTCTGAGAACTGCGAGACATCGAC
  CATGAACATCGACCGCAACATCACCCATCTGCAGCACTGCACGTGTGTGGATGACTGCTCCAGCTCCAAT
  TGCCTATGTGGTCAGCTCAGTATCCGATGCTGGTATGACAAGGACGGGCGGCTGCTCCAGGAGTTTAACA
  AGATCGAGCCCCCCCTGATCTTTGAGTGTAACCAGGCATGCTCCTGCTGGAGAAGCTGCAAGAACCGCGT
  GGTGCAGAGCGGCATCAAGGTACGGCTGCAGCTCTACCGGACTGCCAAGATGGGCTGGGGGGTCCGAGCC
  TTGCAGACCATCCCCCAGGGCACGTTCATCTGCGAGTATGTAGGAGAGCTGATCTCTGATGCCGAGGCTG
  ATGTGAGAGAGGATGATTCTTACCTCTTCGATTTAGATAACAAGGATGGCGAGGTTTACTGCATTGATGC
  CCGTTACTATGGCAACATCAGCCGATTCATTAACCACCTGTGTGACCCCAACATCATCCCTGTCCGGGTT
  TTCATGCTGCACCAAGATCTACGGTTCCCACGCATTGCCTTCTTCAGCTCCAGGGACATCCGGACTGGGG
  AGGAGCTGGGCTTTGACTACGGTGACCGATTCTGGGACATCAAGAGCAAGTATTTCACCTGCCAGTGTGG
  CTCTGAGAAGTGCAAGCATTCAGCGGAGGCCATCGCCCTGGAGCAGAGCCGCCTGGCCCGGCTGGACCCC
  CACCCGGAGCTGCTCCCTGACCTCAGCTCCCTGCCCCCCATCAACACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231680 representing NM_001286575
  Red=Cloning site Green=Tags(s)
  
  MAAAAGAAAAAAAEGEAPAEMGALLLEKEPRGAAERVHSSLGDTPQSEETLPKANPDSLEPAGPSSPASV
  TVTVGDEGADTPVGAASLIGDEPESLEGDGGRIVLGHATKSFPSSPSKGGACPSRAKMSMTGAGKSPPSV
  QSLAMRLLSMPGAQGAATAGPEPSPATTAAQEGQPKVHRARKTMSKPSNGQPPIPEKRPPEVQHFRMSDD
  MHLGKVTSDVAKRRKLNSGSLSEDLGSAGGSGDIILEKGEPRPLEEWETVVGDDFSLYYDAYSVDERVDS
  DSKSEVEALAEQLSEEEEEEEEEEEEEEEEEEEEEEEEEDEESGNQSDRSGSSGRRKAKKKWRKDSPWVK
  PSRKRRKREPPRAKEPRGVNGVGSSGPSEYMEVPLGSLELPSEGTLSPNHAGVSNDTSSLETERGFEELP
  LCSCRMEAPKIDRISERAGHKCMATESVDGELLGCNAAILKRETMRPSSRVALMVLCEAHRARMVKHHCC
  PGCGYFCTAGTFLECHPDFRVAHRFHKACVSQLNGMVFCPHCGEDASEAQEVTIPRGDGGTPPIGTAAPA
  LPPLAHDAPGRADTSQPSARMRGHGEPRRPPCDPLADTIDSSGPSLTLPNGGCLSAVGLPPGPGREALEK
  ALVIQESERRKKLRFHPRQLYLSVKQGELQKVILMLLDNLDPNFQSDQQSKRTPLHAAAQKGSVEICHVL
  LQAGANINAVDKQQRTPLMEAVVNNHLEVARYMVQLGGCVYSKEEDGSTCLHHAAKIGNLEMVSLLLSTG
  QVDVNAQDSGGWTPIIWAAEHKHIDVIRMLLTRGADVTLTDNEENICLHWASFTGSAAIAEVLLNAQCDL
  HAVNYHGDTPLHIAARESYHDCVLLFLSRGANPELRNKEGDTAWDLTPERSDVWFALQLNRKLRLGVGNR
  AVRTEKIICRDVARGYENVPIPCVNGVDGEPCPEDYKYISENCETSTMNIDRNITHLQHCTCVDDCSSSN
  CLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECNQACSCWRSCKNRVVQSGIKVRLQLYRTAKMGWGVRA
  LQTIPQGTFICEYVGELISDAEADVREDDSYLFDLDNKDGEVYCIDARYYGNISRFINHLCDPNIIPVRV
  FMLHQDLRFPRIAFFSSRDIRTGEELGFDYGDRFWDIKSKYFTCQCGSEKCKHSAEAIALEQSRLARLDP
  HPELLPDLSSLPPINT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286575
• ORF Size: 3618 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286575.2
• RefSeq Size: 3907 bp
• RefSeq ORF: 3621 bp
• Locus ID: 110147
• UniProt ID: Q9Z148
• Cytogenetics: 17 18.45 cM
• MW: 132.2 kDa
• Gene Summary: Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.[UniProtKB/Swiss-Prot Function]