Nup98 (NM_001287165) Mouse Tagged ORF Clone

CAT#: MR231648

Nup98 (myc-DDK-tagged) - Mouse nucleoporin 98 (Nup98), transcript variant 3

Product Images

Circular map for MR231648

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nup98
• Synonyms: 4732457F17; AI849286; Nup96
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231648 representing NM_001287165
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTAACAAATCATTTGGAACCCCCTTTGGGGGTAGTACAGGGGGCTTTGGCACAACGTCAACATTTG
  GGCAAAATACTGGCTTTGGTACGACTAGTGGAGGAGCATTTGGAACATCTGCATTTGGTTCTAGCAACAA
  TACTGGAGGCTTATTTGGAAATTCACAGACCAAACCAGGAGGATTATTTGGTACCAGTTCATTTAGCCAG
  CCAGCAACCTCCACAAGCACTGGGTTTGGGTTTGGCACATCAACAGGAACATCAAATAGCTTATTTGGAA
  CTGCAAGTACCGGGACCAGTCTTTTCTCATCCCAGAACAATGCATTTGCACAAAATAAACCAACTGGCTT
  TGGGAATTTTGGAACCAGTACTAGCAGTGGAGGACTCTTTGGAACTACAAATACCACCTCTAATCCTTTT
  GGTAGCACATCTGGCTCCCTTTTTGGGCCAAGTAGTTTTACAGCAGCACCTACAGGAACTACCATCAAAT
  TTAATCCTCCCACTGGTACAGATACTATGGTCAAAGCTGGAGTTAGCACTAACATCAGTACAAAGCATCA
  GTGTATTACTGCTATGAAAGAATATGAAAGCAAGTCATTAGAGGAACTACGTTTGGAGGATTATCAGGCT
  AACCGGAAAGGCCCACAGAACCAAGTGGGAGGAGGCACCACGGCTGGCTTATTTGGGTCTTCTCCAGCAA
  CTTCCAGTGCAACAGGGCTCTTCAGCTCCTCCACCACTAATTCAGCCTTTTCATATGGTCAGAACAAAAC
  TGCCTTTGGAACTAGCACAACTGGATTTGGAACAAATCCAGGTGGTCTCTTTGGCCAACAGAATCAACAG
  ACTACCAGTCTCTTCAGCAAACCATTTGGCCAGGCTACAACCACCCCGAATACTGGCTTTTCCTTTGGTA
  ATACCAGCACCCTTGGACAGCCAAGCACCAATACTATGGGCCTATTTGGAGTAACCCAAGCCTCACAACC
  AGGAGGTCTTTTTGGGACAGCTACAAACACCAGCACTGGGACAGCATTTGGGACAGGAACAGGTCTCTTT
  GGGCAGCCCAATACTGGATTTGGTGCAGTTGGTTCGACCCTGTTTGGCAATAACAAGCTTACAACTTTTG
  GAACCAGCACAACCAGTGCTCCTTCATTTGGTACAACCAGTGGCGGGCTCTTCGGGTTTGGCACAAATAA
  CAGTGGGAGCAGTATTTTTGGAAGTAAGCCAGCAGCTGGAACTTTGGGAACTGGACTTGGTACAGGATTT
  GGAACAGCTCTTGGTGCTGGACAGGCATCTTTGTTTGGAAACAACCAACCTAAGATTGGAGGGCCTCTTG
  GTACAGGAGCCTTTGGGGCCCCTGGATTTAATACTTCGACAGCCATTTTGGGCTTTGGCGCCCCCCAGGC
  CCCAGTAGCTTTGACAGATCCAAATGCTTCTGCTGCCCAGCAGGCTGTTCTCCAGCAGCACCTCAATAGC
  CTAACATACTCACCCTTTGGAGACTCCCCCCTCTTCCGGAATCCTATGTCAGATCCTAAGAAGAAAGAAG
  AGAGACTGAAACCAACCAATCCAGCTGCTCAGAAAGCTCTTACAACACCTACTCATTATAAACTTACACC
  TCGCCCTGCTACCAGAGTCAGGCCAAAGGCTTTGCAAACAACAGGCACAGCCAAATCACATCTCTTTGAT
  GGGCTGGATGACGATGAACCATCTCTAGCCAACGGAGCATTCATGCCTAAAAAGAGCATCAAGAAGTTGG
  TTTTGAAAAATCTCAACAATAGCAATCTCTTTTCTCCTGTTAATCATGATTCAGAAGATCTAGCTTCACC
  CTCTGAGTATCCAGAAAATGGAGAAAGATTTAGCTTCCTGAGCAAACCTGTTGATGAGAACAATCAGCAG
  GATGGAGAAGATGACTCTCTTGTATCACGATTTTACACTAATCCTATTGCCAAACCCATTCCACAAACTC
  CAGAGAGTGTTGGAAACAAAAATAACAGTAGCAGCAATGTGGAAGATACCATTGTTGCCTTGAACATGCG
  TGCTGCTTTGCGCAATGGATTGGAAGGAAGCAGTGAAGAGACGTCATTCCATGATGAGTCATTGCAAGAT
  GACCGAGAAGAGATAGAAAATAATGCTTACCACATACACCCAGCAGGCATTGTTCTCACAAAAGTTGGTT
  ATTACACTATCCCATCTATGGATGACCTTGCTAAAATTACCAACGAGAAGGGAGAATGCATTGTTTCTGA
  CTTCACCATTGGTCGTAAAGGATATGGCTCAATCTATTTTGAAGGAGATGTGAATTTGACAAATCTAAAT
  TTGGATGATATTGTGCATATCCGAAGGAAAGAAGTTATTGTCTATGTAGATGATAACCAAAAGCCACCTG
  TGGGTGAAGGGCTAAATAGGAAGGCTGAAGTTACTTTGGATGGAGTTTGGCCAACAGATAAAACATCCCG
  GTGTTTAATAAAGAGTCCAGATCGACTTGCTGATATCAACTATGAGGGGAGATTAGAAGCAGTCTCAAGA
  AAGCAAGGGGCCCAATTCAAGGAGTATCGGCCTGAAACTGGTTCTTGGGTATTTAAGGTCTCCCATTTTT
  CCAAGTATGGCCTTCAGGATTCTGATGAAGAGGAGGAGGAACACCCACCCAAAACGACTTCAAAGAAGCT
  GAAGACTGCCCCTTTGCCCCCTGCAGGCCAGGCAACCACTTTCCAGATGACTCTTAATGGCAAGCCTGCA
  CCCCCACCTCAGAGCCAGAGCCCAGAAGTGGAGCAGTTAGGCAGGGTTGTGGAACTGGACAGCGACATGG
  TAGATATCACCCAGGAGCCAGTTCCAGATTCTGTGTTAGAAGAGAGTGTGCCCGAGGATCAGGAGCCTGT
  GTCTGCTTCAACGCATATTGCATCTTCACTGGGAATTAATCCACATGTTTTACAGATCATGAAAGCATCA
  TTGCTTGTTGACGAAGAAGATGTAGATGCCATGGATCAACGCTTTGGTCACATCCCTTCCAAAGGAGAGA
  CTGTCCAGGAAATCTGTTCTCCTAGACTCCCCATTTCAGCCTCCCACTCATCAAAATCCCGCTCCATAGT
  TGGTGGGTTGCTGCAATCAAAATTTGCAAGTGGAACTTTTCTTTCACCAAGTGCCTCCGTGCAAGAATGT
  CGCACTCCCAGGACATCATCTCGCATGAACATCCCATCCACATCCCCCTGGTCTGTCCCTCTGCCCCTGG
  CCACTGTGTTCACAGTGCCCAGCCCAGCCCCTGAGGTTCAGCTAAAAACAGTGGGGATACGAAGGCAACC
  AGGCCTAGTCCCTCTTGAAAAATCCATTACATATGGCAAGGGGAAGCTCTTGATGGACATGGCCCTATTC
  ATGGGACGTTCATTTCGGGTTGGTTGGGGTCCCAACTGGACTCTTGCTAATAGTGGAGAACAACTGCATG
  GCTCCCATGAACTGGAAAATCATCAGGTTGCCGATTCTATGGAATATGGATTCCTGCCCAATCCAGTAGC
  TGTTAAATCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231648 representing NM_001287165
  Red=Cloning site Green=Tags(s)
  
  MFNKSFGTPFGGSTGGFGTTSTFGQNTGFGTTSGGAFGTSAFGSSNNTGGLFGNSQTKPGGLFGTSSFSQ
  PATSTSTGFGFGTSTGTSNSLFGTASTGTSLFSSQNNAFAQNKPTGFGNFGTSTSSGGLFGTTNTTSNPF
  GSTSGSLFGPSSFTAAPTGTTIKFNPPTGTDTMVKAGVSTNISTKHQCITAMKEYESKSLEELRLEDYQA
  NRKGPQNQVGGGTTAGLFGSSPATSSATGLFSSSTTNSAFSYGQNKTAFGTSTTGFGTNPGGLFGQQNQQ
  TTSLFSKPFGQATTTPNTGFSFGNTSTLGQPSTNTMGLFGVTQASQPGGLFGTATNTSTGTAFGTGTGLF
  GQPNTGFGAVGSTLFGNNKLTTFGTSTTSAPSFGTTSGGLFGFGTNNSGSSIFGSKPAAGTLGTGLGTGF
  GTALGAGQASLFGNNQPKIGGPLGTGAFGAPGFNTSTAILGFGAPQAPVALTDPNASAAQQAVLQQHLNS
  LTYSPFGDSPLFRNPMSDPKKKEERLKPTNPAAQKALTTPTHYKLTPRPATRVRPKALQTTGTAKSHLFD
  GLDDDEPSLANGAFMPKKSIKKLVLKNLNNSNLFSPVNHDSEDLASPSEYPENGERFSFLSKPVDENNQQ
  DGEDDSLVSRFYTNPIAKPIPQTPESVGNKNNSSSNVEDTIVALNMRAALRNGLEGSSEETSFHDESLQD
  DREEIENNAYHIHPAGIVLTKVGYYTIPSMDDLAKITNEKGECIVSDFTIGRKGYGSIYFEGDVNLTNLN
  LDDIVHIRRKEVIVYVDDNQKPPVGEGLNRKAEVTLDGVWPTDKTSRCLIKSPDRLADINYEGRLEAVSR
  KQGAQFKEYRPETGSWVFKVSHFSKYGLQDSDEEEEEHPPKTTSKKLKTAPLPPAGQATTFQMTLNGKPA
  PPPQSQSPEVEQLGRVVELDSDMVDITQEPVPDSVLEESVPEDQEPVSASTHIASSLGINPHVLQIMKAS
  LLVDEEDVDAMDQRFGHIPSKGETVQEICSPRLPISASHSSKSRSIVGGLLQSKFASGTFLSPSASVQEC
  RTPRTSSRMNIPSTSPWSVPLPLATVFTVPSPAPEVQLKTVGIRRQPGLVPLEKSITYGKGKLLMDMALF
  MGRSFRVGWGPNWTLANSGEQLHGSHELENHQVADSMEYGFLPNPVAVKS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001287165
• ORF Size: 3510 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001287165.1, NP_001274094.1
• RefSeq Size: 4086 bp
• RefSeq ORF: 3513 bp
• Locus ID: 269966
• UniProt ID: Q6PFD9
• Cytogenetics: 7 54.71 cM
• MW: 123.5 kDa
• Gene Summary: Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. NUP98 and NUP96 are involved in the bidirectional transport across the NPC. May anchor NUP153 and TPR to the NPC. In cooperation with DHX9, plays a role in transcription and alternative splicing activation of a subset of genes. Involved in the localization of DHX9 in discrete intranuclear foci (GLFG-body).[UniProtKB/Swiss-Prot Function]