Nup98 (NM_001287165) Mouse Untagged Clone

CAT#: MC229437

Nup98 (untagged) - Mouse nucleoporin 98 (Nup98), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nup98
• Synonyms: 4732457F17; AI849286; Nup96
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229437 representing NM_001287165
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTAACAAATCATTTGGAACCCCCTTTGGGGGTAGTACAGGGGGCTTTGGCACAACGTCAACATTTG
  GGCAAAATACTGGCTTTGGTACGACTAGTGGAGGAGCATTTGGAACATCTGCATTTGGTTCTAGCAACAA
  TACTGGAGGCTTATTTGGAAATTCACAGACCAAACCAGGAGGATTATTTGGTACCAGTTCATTTAGCCAG
  CCAGCAACCTCCACAAGCACTGGGTTTGGGTTTGGCACATCAACAGGAACATCAAATAGCTTATTTGGAA
  CTGCAAGTACCGGGACCAGTCTTTTCTCATCCCAGAACAATGCATTTGCACAAAATAAACCAACTGGCTT
  TGGGAATTTTGGAACCAGTACTAGCAGTGGAGGACTCTTTGGAACTACAAATACCACCTCTAATCCTTTT
  GGTAGCACATCTGGCTCCCTTTTTGGGCCAAGTAGTTTTACAGCAGCACCTACAGGAACTACCATCAAAT
  TTAATCCTCCCACTGGTACAGATACTATGGTCAAAGCTGGAGTTAGCACTAACATCAGTACAAAGCATCA
  GTGTATTACTGCTATGAAAGAATATGAAAGCAAGTCATTAGAGGAACTACGTTTGGAGGATTATCAGGCT
  AACCGGAAAGGCCCACAGAACCAAGTGGGAGGAGGCACCACGGCTGGCTTATTTGGGTCTTCTCCAGCAA
  CTTCCAGTGCAACAGGGCTCTTCAGCTCCTCCACCACTAATTCAGCCTTTTCATATGGTCAGAACAAAAC
  TGCCTTTGGAACTAGCACAACTGGATTTGGAACAAATCCAGGTGGTCTCTTTGGCCAACAGAATCAACAG
  ACTACCAGTCTCTTCAGCAAACCATTTGGCCAGGCTACAACCACCCCGAATACTGGCTTTTCCTTTGGTA
  ATACCAGCACCCTTGGACAGCCAAGCACCAATACTATGGGCCTATTTGGAGTAACCCAAGCCTCACAACC
  AGGAGGTCTTTTTGGGACAGCTACAAACACCAGCACTGGGACAGCATTTGGGACAGGAACAGGTCTCTTT
  GGGCAGCCCAATACTGGATTTGGTGCAGTTGGTTCGACCCTGTTTGGCAATAACAAGCTTACAACTTTTG
  GAACCAGCACAACCAGTGCTCCTTCATTTGGTACAACCAGTGGCGGGCTCTTCGGGTTTGGCACAAATAA
  CAGTGGGAGCAGTATTTTTGGAAGTAAGCCAGCAGCTGGAACTTTGGGAACTGGACTTGGTACAGGATTT
  GGAACAGCTCTTGGTGCTGGACAGGCATCTTTGTTTGGAAACAACCAACCTAAGATTGGAGGGCCTCTTG
  GTACAGGAGCCTTTGGGGCCCCTGGATTTAATACTTCGACAGCCATTTTGGGCTTTGGCGCCCCCCAGGC
  CCCAGTAGCTTTGACAGATCCAAATGCTTCTGCTGCCCAGCAGGCTGTTCTCCAGCAGCACCTCAATAGC
  CTAACATACTCACCCTTTGGAGACTCCCCCCTCTTCCGGAATCCTATGTCAGATCCTAAGAAGAAAGAAG
  AGAGACTGAAACCAACCAATCCAGCTGCTCAGAAAGCTCTTACAACACCTACTCATTATAAACTTACACC
  TCGCCCTGCTACCAGAGTCAGGCCAAAGGCTTTGCAAACAACAGGCACAGCCAAATCACATCTCTTTGAT
  GGGCTGGATGACGATGAACCATCTCTAGCCAACGGAGCATTCATGCCTAAAAAGAGCATCAAGAAGTTGG
  TTTTGAAAAATCTCAACAATAGCAATCTCTTTTCTCCTGTTAATCATGATTCAGAAGATCTAGCTTCACC
  CTCTGAGTATCCAGAAAATGGAGAAAGATTTAGCTTCCTGAGCAAACCTGTTGATGAGAACAATCAGCAG
  GATGGAGAAGATGACTCTCTTGTATCACGATTTTACACTAATCCTATTGCCAAACCCATTCCACAAACTC
  CAGAGAGTGTTGGAAACAAAAATAACAGTAGCAGCAATGTGGAAGATACCATTGTTGCCTTGAACATGCG
  TGCTGCTTTGCGCAATGGATTGGAAGGAAGCAGTGAAGAGACGTCATTCCATGATGAGTCATTGCAAGAT
  GACCGAGAAGAGATAGAAAATAATGCTTACCACATACACCCAGCAGGCATTGTTCTCACAAAAGTTGGTT
  ATTACACTATCCCATCTATGGATGACCTTGCTAAAATTACCAACGAGAAGGGAGAATGCATTGTTTCTGA
  CTTCACCATTGGTCGTAAAGGATATGGCTCAATCTATTTTGAAGGAGATGTGAATTTGACAAATCTAAAT
  TTGGATGATATTGTGCATATCCGAAGGAAAGAAGTTATTGTCTATGTAGATGATAACCAAAAGCCACCTG
  TGGGTGAAGGGCTAAATAGGAAGGCTGAAGTTACTTTGGATGGAGTTTGGCCAACAGATAAAACATCCCG
  GTGTTTAATAAAGAGTCCAGATCGACTTGCTGATATCAACTATGAGGGGAGATTAGAAGCAGTCTCAAGA
  AAGCAAGGGGCCCAATTCAAGGAGTATCGGCCTGAAACTGGTTCTTGGGTATTTAAGGTCTCCCATTTTT
  CCAAGTATGGCCTTCAGGATTCTGATGAAGAGGAGGAGGAACACCCACCCAAAACGACTTCAAAGAAGCT
  GAAGACTGCCCCTTTGCCCCCTGCAGGCCAGGCAACCACTTTCCAGATGACTCTTAATGGCAAGCCTGCA
  CCCCCACCTCAGAGCCAGAGCCCAGAAGTGGAGCAGTTAGGCAGGGTTGTGGAACTGGACAGCGACATGG
  TAGATATCACCCAGGAGCCAGTTCCAGATTCTGTGTTAGAAGAGAGTGTGCCCGAGGATCAGGAGCCTGT
  GTCTGCTTCAACGCATATTGCATCTTCACTGGGAATTAATCCACATGTTTTACAGATCATGAAAGCATCA
  TTGCTTGTTGACGAAGAAGATGTAGATGCCATGGATCAACGCTTTGGTCACATCCCTTCCAAAGGAGAGA
  CTGTCCAGGAAATCTGTTCTCCTAGACTCCCCATTTCAGCCTCCCACTCATCAAAATCCCGCTCCATAGT
  TGGTGGGTTGCTGCAATCAAAATTTGCAAGTGGAACTTTTCTTTCACCAAGTGCCTCCGTGCAAGAATGT
  CGCACTCCCAGGACATCATCTCGCATGAACATCCCATCCACATCCCCCTGGTCTGTCCCTCTGCCCCTGG
  CCACTGTGTTCACAGTGCCCAGCCCAGCCCCTGAGGTTCAGCTAAAAACAGTGGGGATACGAAGGCAACC
  AGGCCTAGTCCCTCTTGAAAAATCCATTACATATGGCAAGGGGAAGCTCTTGATGGACATGGCCCTATTC
  ATGGGACGTTCATTTCGGGTTGGTTGGGGTCCCAACTGGACTCTTGCTAATAGTGGAGAACAACTGCATG
  GCTCCCATGAACTGGAAAATCATCAGGTTGCCGATTCTATGGAATATGGATTCCTGCCCAATCCAGTAGC
  TGTTAAATCGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001287165
• Insert Size: 3513 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001287165.1, NP_001274094.1
• RefSeq Size: 4086 bp
• RefSeq ORF: 3513 bp
• Locus ID: 269966
• UniProt ID: Q6PFD9
• Cytogenetics: 7 54.71 cM
• Gene Summary: Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. NUP98 and NUP96 are involved in the bidirectional transport across the NPC. May anchor NUP153 and TPR to the NPC. In cooperation with DHX9, plays a role in transcription and alternative splicing activation of a subset of genes. Involved in the localization of DHX9 in discrete intranuclear foci (GLFG-body).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) has multiple coding region differences compared to variant 1 and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' UTR, compared to variant 1. It encodes isoform 3 which is shorter and has a distinct C-terminus, compared to isoform 1.