Grin3a (NM_001276355) Mouse Tagged ORF Clone

CAT#: MR231614

Grin3a (myc-DDK-tagged) - Mouse glutamate receptor ionotropic, NMDA3A (Grin3a), transcript variant 1

Product Images

Circular map for MR231614

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Grin3a
• Synonyms: 6430537F04; A830097C19Rik; mKIAA1973; NMDAR-L; NR3A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231614 representing NM_001276355
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAGACTGAGTTTGTGGTGGCTGCTGAGCAGGGTCTGTCTGTTGCTGCCTCCGCCCTGTGCACTGG
  TGCTGGCCGGGGTGCCCAGCTCCTCCTCGCACCCGCAACCCTGCCAGATCCTCAAGCGCATCGGACACGC
  GGTGAGGGTGGGCGCGGTGCACTTGCAACCCTGGACCACGGCCCCACGCGCAGCCAGTCGCGCTCAGGAC
  GGCGGCAGGGCGGGTGCCCAGAGGGATGAGCCAGAGTCGGGGACGTGGCGGCCACCGGCGCCCTCGCAAG
  GCGCACGTTGGTTGGGGAGCGCCCTGCATGGCCGGGGTCCACCCGGCTCCCGCAAGCTCGGGGAGGGCGC
  CGGGACCGAGACCCTGTGGCCGCGGGATGCCCTACTGTTCGCTGTGGAAAACCTGAACCGTGTGGAAGGG
  CTCCTTCCCTACAACCTGTCTTTGGAAGTAGTGATGGCCATCGAGGCGGGCCTGGGCGACCTGCCACTTA
  TGCCTTTCTCTTCCCCAAGCTCACCGTGGAGCAGTGACCCTTTCTCCTTTCTGCAGAGCGTGTGCCACAC
  CGTAGTGGTACAAGGGGTTTCAGCGCTGCTGGCCTTCCCCCAGAGCCAGGGCGAAATGATGGAGCTGGAC
  TTGGTCAGTTCTGTCCTGCACATCCCAGTGCTCAGCATAGTGCGCCACGAGTTTCCGCGGGAGAGTCAGA
  ATCCCCTGCACCTACAGCTGAGTTTAGAAAATTCACTAAGTTCTGATGCTGATGTCACTGTCTCAATCCT
  GACCATGAACAACTGGTACAATTTTAGCTTGTTGCTATGCCAGGAAGACTGGAATATCACCGACTTCCTA
  CTCCTTACAGAGAATAACTCCAAGTTCCACCTCGAGTCTATCATCAACATCACTGCTAACCTGTCCTCCA
  CAAAGGACCTTCTAAGTTTCCTGCAGGTCCAGCTGGAAAACATTAGGAACAGCACACCCACAATGGTGAT
  GTTTGGCTGTGACATGGGGAGCATCCGGCAGATATTTGAAATGTCCACACAGTTTGGGCTATCACCTCCT
  GATCTTCACTGGGTTTTAGGAGACTCACAGAATGTGGAGGAGCTGAGGACAGAAGGCCTGCCCTTAGGGC
  TCATTGCTCATGGAAAAACCACACAGTCTGTCTTTGAGTACTATGTTCAGGATGCCATGGAGTTGGTTGC
  AAGAGCTGTAGCCACAGCCACCATGATCCAGCCAGAACTTGCTCTCCTTCCCAGCACAATGAACTGCATG
  GATGTGAAAACCACAAATCTCACTTCTGGACAATATTTATCAAGGTTTTTAGCCAACACCACTTTCAGAG
  GTCTCAGTGGTTCCATCAAAGTAAAGGGATCCACCATCGTCAGCTCAGAAAACAACTTTTTCATCTGGAA
  CTTGCAGTATGACCCTATGGGAAAGCCAATGTGGACTCGCCTGGGTAGCTGGCAAGGGGGAAGGATTGTC
  ATGGACTCGGGAATATGGCCAGAGCAGGCCCAGAGGCACAAAACCCACTTCCATCACCCAAACAAGTTAC
  ACTTGAGAGTGGTGACACTGATTGAACATCCATTTGTTTTCACGAGAGAAGTAGATGATGAAGGCTTATG
  CCCTGCTGGACAACTCTGTCTAGACCCTATGACTAATGACTCTTCCATACTGGATAGCCTGTTTAGCAGC
  CTACATAGCAGTAATGATACAGTGCCAATCAAGTTCAAGAAGTGCTGCTATGGGTATTGCATTGATCTAC
  TGGAACAGTTAGCAGAAGACATGAACTTTGACTTTGACCTCTATATTGTAGGGGATGGAAAGTATGGAGC
  TTGGAAAAATGGTCACTGGACTGGGCTGGTTGGTGATCTCCTGAGTGGAACAGCCAACATGGCAGTCACT
  TCTTTCAGCATCAATACTGCAAGAAGCCAAGTGATAGATTTCACCAGCCCTTTCTTCTCAACCAGTTTGG
  GCATCTTAGTGAGGACTCGAGACACAGCAGCTCCCATTGGAGCCTTCATGTGGCCACTCCACTGGACCAT
  GTGGCTGGGGATTTTCGTGGCTCTACATATCACTGCCATCTTCCTCACTCTGTATGAATGGAAGAGCCCC
  TTTGGTATGACTCCTAAAGGGAGGAACAGAAACAAAGTCTTCTCCTTCTCCTCAGCCTTGAATGTCTGCT
  ATGCCCTTCTGTTTGGCAGAACAGCAGCCATCAAACCCCCTAAATGCTGGACTGGAAGATTTCTGATGAA
  TCTTTGGGCCATTTTCTGTATGTTTTGCCTTTCTACATACACAGCGAACTTGGCTGCTGTCATGGTAGGT
  GAGAAGATCTATGAAGAACTTTCTGGAATCCATGACCCTAAGCTTCATCATCCTTCTCAAGGTTTTCGTT
  TTGGAACTGTCCGGGAAAGCAGTGCTGAGGACTATGTACGGCAGAGCTTCCCAGAGATGCATGAGTACAT
  GAGAAGGTACAATGTACCAGCCACCCCTGATGGAGTGCAGTACCTGAAGAATGATCCAGAGAAACTAGAT
  GCCTTCATCATGGACAAAGCCCTTCTGGATTATGAAGTGTCAATAGATGCTGACTGCAAGCTTCTGACCG
  TAGGAAAGCCATTTGCCATTGAAGGCTATGGCATTGGTCTCCCTCCCAACTCTCCGTTGACCTCTAACAT
  ATCTGAGCTCATCAGTCAGTACAAGTCTCATGGGTTTATGGATGTGCTTCATGACAAGTGGTACAAGGTG
  GTTCCCTGCGGAAAGAGAAGCTTTGCTGTCACTGAGACTTTGCAAATGGGCATCAAGCATTTCTCTGGAC
  TCTTCGTGCTGCTGTGCATAGGATTCGGTCTCTCCATCCTGACAACCATTGGTGAACACATAGTGTACAG
  ACTGCTGCTACCACGAATCAAAAACAAATCCAAGCTGCAGTACTGGCTGCACACCAGTCAGAGGTTTCAC
  AGAGCATTAAACACATCATTTGTAGAAGAAAAGCAGCCATGTTCCAAGACAAAACGTGTGGAAAAGAGCA
  GATGGAGAAGATGGACCTGCAAGACAGAAGGGGACTCTGAATTGTCTCTGTTTCCTAGGTCCAACATGGG
  ACCCCAGCAGCTCATGGTATGGAATACTTCCAATCTGAGTCACGACAACCAACGAAAATACATCTTTAAT
  GATGAGGAAGGACAAAACCAGCTGGGTACCCAGACCCATCAGGACATCCCTCTCCCTCCAAGGAGAAGAG
  AGCTCCCTGCTTCACTGACCACCAATGGGAAAGCAGACTCCCTCAATGTAGCTCGGAACTCCGTGATGCA
  GGAACTCTCCGAGTTGGAGAAGCAGATCCAAGTGATCCGCCAGGAGCTGCAGTTGGCCGTAAGCAGGAAG
  ACAGAGCTTGAGGAGTATCAAAGGACGAACCGGACTTGTGAATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231614 representing NM_001276355
  Red=Cloning site Green=Tags(s)
  
  MRRLSLWWLLSRVCLLLPPPCALVLAGVPSSSSHPQPCQILKRIGHAVRVGAVHLQPWTTAPRAASRAQD
  GGRAGAQRDEPESGTWRPPAPSQGARWLGSALHGRGPPGSRKLGEGAGTETLWPRDALLFAVENLNRVEG
  LLPYNLSLEVVMAIEAGLGDLPLMPFSSPSSPWSSDPFSFLQSVCHTVVVQGVSALLAFPQSQGEMMELD
  LVSSVLHIPVLSIVRHEFPRESQNPLHLQLSLENSLSSDADVTVSILTMNNWYNFSLLLCQEDWNITDFL
  LLTENNSKFHLESIINITANLSSTKDLLSFLQVQLENIRNSTPTMVMFGCDMGSIRQIFEMSTQFGLSPP
  DLHWVLGDSQNVEELRTEGLPLGLIAHGKTTQSVFEYYVQDAMELVARAVATATMIQPELALLPSTMNCM
  DVKTTNLTSGQYLSRFLANTTFRGLSGSIKVKGSTIVSSENNFFIWNLQYDPMGKPMWTRLGSWQGGRIV
  MDSGIWPEQAQRHKTHFHHPNKLHLRVVTLIEHPFVFTREVDDEGLCPAGQLCLDPMTNDSSILDSLFSS
  LHSSNDTVPIKFKKCCYGYCIDLLEQLAEDMNFDFDLYIVGDGKYGAWKNGHWTGLVGDLLSGTANMAVT
  SFSINTARSQVIDFTSPFFSTSLGILVRTRDTAAPIGAFMWPLHWTMWLGIFVALHITAIFLTLYEWKSP
  FGMTPKGRNRNKVFSFSSALNVCYALLFGRTAAIKPPKCWTGRFLMNLWAIFCMFCLSTYTANLAAVMVG
  EKIYEELSGIHDPKLHHPSQGFRFGTVRESSAEDYVRQSFPEMHEYMRRYNVPATPDGVQYLKNDPEKLD
  AFIMDKALLDYEVSIDADCKLLTVGKPFAIEGYGIGLPPNSPLTSNISELISQYKSHGFMDVLHDKWYKV
  VPCGKRSFAVTETLQMGIKHFSGLFVLLCIGFGLSILTTIGEHIVYRLLLPRIKNKSKLQYWLHTSQRFH
  RALNTSFVEEKQPCSKTKRVEKSRWRRWTCKTEGDSELSLFPRSNMGPQQLMVWNTSNLSHDNQRKYIFN
  DEEGQNQLGTQTHQDIPLPPRRRELPASLTTNGKADSLNVARNSVMQELSELEKQIQVIRQELQLAVSRK
  TELEEYQRTNRTCES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001276355
• ORF Size: 3405 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276355.1, NP_001263284.1
• RefSeq Size: 7727 bp
• RefSeq ORF: 3408 bp
• Locus ID: 242443
• UniProt ID: A2AIR4
• Cytogenetics: 4 B1
• MW: 128 kDa
• Gene Summary: NMDA receptor subtype of glutamate-gated ion channels with reduced single-channel conductance, low calcium permeability and low voltage-dependent sensitivity to magnesium. Mediated by glycine. During the development of neural circuits, plays a role in the synaptic refinement period, restricting spine maturation and growth (By similarity). By competing with GIT1 interaction with ARHGEF7/beta-PIX, may reduce GIT1/ARHGEF7-regulated local activation of RAC1, hence affecting signaling and limiting the maturation and growth of inactive synapses (PubMed:24297929). May also play a role in PPP2CB-NMDAR mediated signaling mechanism (By similarity).[UniProtKB/Swiss-Prot Function]