Aebp1 (NM_001291857) Mouse Tagged ORF Clone

CAT#: MR231609

Aebp1 (myc-DDK-tagged) - Mouse AE binding protein 1 (Aebp1), transcript variant 1

Product Images

Circular map for MR231609

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aebp1
• Synonyms: ACLP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231609 representing NM_001291857
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCCAGTGCGCACCGCATCCCTGCTCTGCGGCCTCCTGGCACTGCTGACGCTGTGCCCTGAGGGGA
  ACCCACAGACGGTGCTGACGGACGACGAGATCGAGGAGTTCCTCGAAGGCTTCCTTTCGGAGTTGGAGAC
  CCAGTCCCCGCCCCGGGAAGACGACGTGGAAGTCCAGCCGCTTCCCGAACCCACCCAGCGTCCCCGCAAA
  TCCAAGGCAGGGGGCAAGCAGCGGGCAGATGTAGAAGTCCCTCCAGAAAAAAACAAAGACAAAGAGAAGA
  AAGGAAAGAAGGACAAAGGCCCCAAAGCCACAAAACCCCTGGAGGGCTCTACCAGGCCCACCAAGAAACC
  AAAGGAGAAGCCACCCAAGGCCACCAAGAAGCCCAAGGAGAAACCACCCAAGGCCACCAAGAAGCCCAAG
  GAGAAGCCACCCAAGGCCACCAAGAAGCCTAAGGAGAAGCCACCCAAGGCCACTAAGAGGCCCTCGGCAG
  GAAAGAAGTTCTCAACTGTGGCCCCCTTGGAAACGCTGGATCGGTTACTCCCCTCACCCTCCAACCCCAG
  CGCCCAGGAGCTACCGCAGAAGAGAGACACACCCTTCCCAAATGCCTGGCAAGGTCAAGGAGAAGAGACC
  CAGGTGGAGGCCAAGCAGCCCCGGCCAGAGCCAGAGGAGGAGACTGAGATGCCCACACTGGACTACAATG
  ACCAGATAGAGAAGGAGGATTACGAGGATTTTGAGTACATCCGTCGCCAGAAGCAGCCCAGGCCAACACC
  CAGCAGGAGGAGGCTCTGGCCAGAGCGCCCTGAGGAGAAGACTGAAGAGCCAGAGGAAAGGAAGGAAGTC
  GAGCCACCTCTGAAGCCCCTGCTGCCTCCGGACTATGGGGATAGCTACGTGATCCCCAACTATGATGACT
  TGGACTATTATTTCCCCCACCCTCCACCGCAGAAGCCTGATGTTGGACAAGAGGTGGATGAGGAAAAGGA
  AGAGATGAAGAAGCCCAAAAAGGAGGGTAGTAGCCCCAAGGAGGACACAGAGGACAAGTGGACCGTGGAG
  AAAAACAAGGACCACAAAGCAGGGCCCCGGAAGGGTGAGGAGCTGGAGGAGGAGTGGGCGCCAGTGGAGA
  AAATCAAGTGCCCACCTATTGGGATGGAGTCACACCGCATTGAGGACAACCAGATCCGTGCCTCCTCCAT
  GCTGCGCCACGGCCTCGGAGCCCAGCGGGGCCGGCTCAACATGCAGGCTGGTGCCAATGAAGATGACTAC
  TATGACGGGGCATGGTGTGCTGAGGACGAGTCGCAGACCCAGTGGATCGAGGTGGACACCCGAAGGACAA
  CTCGGTTCACGGGCGTCATCACTCAGGGCCGTGACTCCAGCATCCATGACGACTTCGTGACTACCTTCTT
  TGTGGGCTTCAGCAATGACAGCCAGACCTGGGTGATGTACACCAATGGCTACGAGGAAATGACCTTCTAT
  GGAAATGTGGACAAGGACACACCTGTGCTGAGCGAGCTCCCTGAGCCAGTTGTGGCCCGTTTCATCCGCA
  TCTATCCACTCACCTGGAACGGTAGCCTGTGCATGCGCCTGGAGGTGCTAGGCTGCCCCGTGACCCCTGT
  CTACAGCTACTACGCACAGAATGAGGTGGTAACTACTGACAGCCTGGACTTCCGGCACCACAGCTACAAG
  GACATGCGCCAGCTGATGAAGGCTGTCAATGAGGAGTGCCCCACAATCACTCGCACATACAGCCTGGGCA
  AGAGTTCACGAGGGCTCAAGATCTACGCAATGGAAATCTCAGACAACCCTGGGGATCATGAACTGGGGGA
  GCCCGAGTTCCGCTACACAGCCGGGATCCACGGCAATGAGGTGCTAGGCCGAGAGCTCCTGCTCCTGCTC
  ATGCAATACCTATGCCAGGAGTACCGCGATGGGAACCCGAGAGTGCGCAACCTGGTGCAGGACACACGCA
  TCCACCTGGTGCCCTCGCTGAACCCTGATGGCTATGAGGTGGCAGCGCAGATGGGCTCAGAGTTTGGGAA
  CTGGGCACTGGGGCTGTGGACTGAGGAGGGCTTTGACATCTTCGAGGACTTCCCAGATCTCAACTCTGTG
  CTCTGGGCAGCTGAGGAGAAGAAATGGGTCCCCTACAGGGTCCCAAACAATAACTTGCCAATCCCTGAAC
  GTTACCTGTCCCCAGATGCCACGGTCTCCACAGAAGTCCGGGCCATTATTTCCTGGATGGAGAAGAACCC
  CTTTGTGCTGGGTGCAAATCTGAACGGTGGTGAGCGGCTTGTGTCTTATCCCTATGACATGGCCCGGACA
  CCTAGCCAGGAGCAGCTGTTGGCCGAGGCACTGGCAGCTGCCCGCGGAGAAGATGATGACGGGGTGTCTG
  AGGCCCAGGAGACTCCAGATCACGCTATTTTCCGCTGGCTGGCCATCTCATTTGCCTCCGCCCATCTCAC
  CATGACGGAGCCCTACCGGGGAGGGTGCCAGGCCCAGGACTACACCAGCGGCATGGGCATTGTCAACGGG
  GCCAAGTGGAATCCTCGCTCTGGGACTTTCAATGACTTTAGCTACCTGCACACAAACTGTCTGGAGCTCT
  CCGTATACCTGGGCTGTGACAAGTTCCCCCACGAGAGTGAGCTACCCCGAGAATGGGAGAACAACAAAGA
  AGCGCTGCTCACCTTCATGGAGCAGGTGCACCGTGGCATTAAGGGTGTGGTGACAGATGAGCAAGGCATC
  CCCATTGCCAATGCCACCATCTCTGTGAGTGGCATCAACCATGGTGTGAAGACAGCAAGTGGAGGTGACT
  ACTGGCGCATTCTGAACCCGGGTGAGTACCGTGTGACAGCTCACGCAGAGGGCTACACCTCAAGTGCCAA
  GATCTGCAATGTGGACTACGATATTGGGGCCACTCAGTGCAACTTCATCCTGGCTCGATCCAACTGGAAG
  CGCATTCGGGAGATCTTGGCTATGAACGGGAACCGTCCCATTCTCCGAGTTGACCCCTCACGACCCATGA
  CCCCCCAGCAGCGGCGCATGCAGCAGCGCCGTCTACAGTACCGGCTCCGCATGAGGGAACAGATGCGACT
  GCGTCGCCTCAATTCTACCGCAGGCCCTGCCACAAGCCCCACTCCTGCCCTTATGCCTCCCCCTTCCCCT
  ACACCAGCCATTACCTTGAGGCCCTGGGAAGTTCTACCCACTACCACTGCAGGCTGGGAGGAGTCAGAGA
  CTGAGACCTATACAGAAGTAGTGACAGAGTTTGAGACAGAGTATGGGACTGACCTAGAGGTGGAAGAGAT
  AGAGGAGGAGGAGGAGGAGGAGGAGGAAGAGATGGACACAGGCCTTACATTTCCACTCACAACAGTGGAG
  ACCTACACAGTGAACTTTGGGGACTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231609 representing NM_001291857
  Red=Cloning site Green=Tags(s)
  
  MAPVRTASLLCGLLALLTLCPEGNPQTVLTDDEIEEFLEGFLSELETQSPPREDDVEVQPLPEPTQRPRK
  SKAGGKQRADVEVPPEKNKDKEKKGKKDKGPKATKPLEGSTRPTKKPKEKPPKATKKPKEKPPKATKKPK
  EKPPKATKKPKEKPPKATKRPSAGKKFSTVAPLETLDRLLPSPSNPSAQELPQKRDTPFPNAWQGQGEET
  QVEAKQPRPEPEEETEMPTLDYNDQIEKEDYEDFEYIRRQKQPRPTPSRRRLWPERPEEKTEEPEERKEV
  EPPLKPLLPPDYGDSYVIPNYDDLDYYFPHPPPQKPDVGQEVDEEKEEMKKPKKEGSSPKEDTEDKWTVE
  KNKDHKAGPRKGEELEEEWAPVEKIKCPPIGMESHRIEDNQIRASSMLRHGLGAQRGRLNMQAGANEDDY
  YDGAWCAEDESQTQWIEVDTRRTTRFTGVITQGRDSSIHDDFVTTFFVGFSNDSQTWVMYTNGYEEMTFY
  GNVDKDTPVLSELPEPVVARFIRIYPLTWNGSLCMRLEVLGCPVTPVYSYYAQNEVVTTDSLDFRHHSYK
  DMRQLMKAVNEECPTITRTYSLGKSSRGLKIYAMEISDNPGDHELGEPEFRYTAGIHGNEVLGRELLLLL
  MQYLCQEYRDGNPRVRNLVQDTRIHLVPSLNPDGYEVAAQMGSEFGNWALGLWTEEGFDIFEDFPDLNSV
  LWAAEEKKWVPYRVPNNNLPIPERYLSPDATVSTEVRAIISWMEKNPFVLGANLNGGERLVSYPYDMART
  PSQEQLLAEALAAARGEDDDGVSEAQETPDHAIFRWLAISFASAHLTMTEPYRGGCQAQDYTSGMGIVNG
  AKWNPRSGTFNDFSYLHTNCLELSVYLGCDKFPHESELPREWENNKEALLTFMEQVHRGIKGVVTDEQGI
  PIANATISVSGINHGVKTASGGDYWRILNPGEYRVTAHAEGYTSSAKICNVDYDIGATQCNFILARSNWK
  RIREILAMNGNRPILRVDPSRPMTPQQRRMQQRRLQYRLRMREQMRLRRLNSTAGPATSPTPALMPPPSP
  TPAITLRPWEVLPTTTAGWEESETETYTEVVTEFETEYGTDLEVEEIEEEEEEEEEEMDTGLTFPLTTVE
  TYTVNFGDF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001291857
• ORF Size: 3387 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291857.2, NP_001278786.1
• RefSeq Size: 4075 bp
• RefSeq ORF: 3390 bp
• Locus ID: 11568
• UniProt ID: Q640N1
• Cytogenetics: 11 A1
• MW: 128.9 kDa
• Gene Summary: Isoform 1: As a positive regulator of collagen fibrillogenesis, it is probably involved in the organization and remodeling of the extracellualr matrix.[UniProtKB/Swiss-Prot Function]