Adamts13 (NM_001290464) Mouse Tagged ORF Clone

CAT#: MR231513

Adamts13 (myc-DDK-tagged) - Mouse a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 13 (Adamts13), transcript variant IAP-a

Product Images

Circular map for MR231513

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adamts13
• Synonyms: ADAM-TS13; ADAMTS-13; Gm710; vWF-CP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231513 representing NM_001290464
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCAGCTTTGCCTGTGGTTGACGTGCCAGCCTTGTTATGCTGTCAGTGTCAGAGGAATCCTCACTG
  GTGCCATCTTCATTCTGGGCTGCTGGGGGCTCTCTGACTTCCAGAAGAGTCTTCTTCAAGATCTGGAGCC
  CAAGGATGTGTCTTCTTACTTTGGCCACCATGCTGCTCCATTCACAGGCCATCCTCCCTCTCACCTCCAG
  AGACTGAGACGGAGAAGGACTTTGGAGGACATTCTGCACCTGGAACTCCTGGTAGCTGTGGGCCCCGATG
  TTTCCCGGGCTCATCAGGAGGACACAGAACGCTACGTGCTCACTAATCTCAATATCGGGTCAGAACTGTT
  GAGAAACCCATCCCTGGGAGTCCAGTTCCAGGTGCACCTGGTGAAGCTAATCACCCTCTCTGACTCAGAG
  AGTACTCCGAATATCACGGCCAACATCACCTCATCCTTGATGAGCGTCTGCGAGTGGAGCCAGACGATCA
  ACCCCCACGATGACAGGGATCCAAGTCACGCTGACCTGATTCTCTATATCACCAGGTTTGACCTGGAGTT
  GCCTGATGGCAACCAGCAGGTTCGGGGTGTCACCCAGCTGGGAGGTGCCTGCTCCCTTTCCTGGAGTTGC
  CTTATCACTGAGGATACTGGCTTTGACCTGGGGGTCACCATCGCCCATGAGATTGGGCACAGCTTCGGGC
  TGGACCATGATGGTGCTCCAGGTAGTGGCAGCACCTGCAAGGCCAGTGGCCACGTGATGGCGGCTGATGG
  CGCAACACCTACTGGAGGGACCCTGGAGTGGTCTGCCTGCAGCCAAAGGCAGTTGCAGCACCTACTCAGC
  ACAGGGCAAATGCACTGCTTCCAGGACCCACCTGGGCTGCAGTCAGGACTTACACGGCACCAGCTGATGG
  CACAGCCTGGCCTCTACTACAGTGCAGATGATCAGTGCCGTGTGGCTTTCGGTTCTGGGGCTGTCGCCTG
  CACCTTCTCCAGGGAGGGTCTGGATGTATGCCAGGCCCTGTCCTGCCACACAGACCCCTTGGACCAAAGC
  AGCTGCAGCCGCCTCCTTGTTCCTCTCCTGGATGGGACAGAATGTGGTGTGGAGAAGTGGTGCTCCAAGG
  CTCGCTGTCGCTCCCTAGCTGAGCTGGCTCCTGTGGCTGCAGTACATGGACACTGGTCTAGCTGGGGCCC
  CCATAGTCCCTGCTCCCGATCCTGTGGAGGAGGTGTGATTACCAGGAGGCGGTGGTGCAACAACCCCAGG
  CCTGCATTTGGGGGACGTGCATGTGTGGGTGAAGACCTCCAGGCTAAGATGTGCAACACGCAGGCTTGTG
  AGAAGACTCAGCTGGAGTTCATGTCCGAGCAGTGTGCCCAGACAGACAGACAACCACTGCAACTTTCCCA
  AGGCACTGCCTCCTTCTACCACTGGGATGCTGCTGTGCAGTATAGTCAAGGAGATACCCTGTGCAGACAC
  ATGTGCTGGGCTGTTGGAGAAAGCTTCATTGTCAGCCGTGGGGACAGGTTCCTAGATGGGACCCGTTGTG
  TGCCAAGTGGTCCTCAGGATGATGGGACCCTAAGCCTCTGTTTGTTGGGCAGCTGCAGGACCTTTGGCTG
  TGATGGCAGGATGGACTCCCAGAAGGTTTGGGATGCGTGCCAGGTGTGTGGAGGAGACAACAGCACCTGC
  AGCTCACGGAATGGTTCTTTCACAGCTGGGAGAGCCAGAGAATATGTCACGTTCCTGATTGTTACTCCCA
  ACATGACCAACGCACACATTGTCAACCGCAGGCCTCTCTTCACACACTTGGCGGTGAGGATCCAGGGCCA
  CTACATTGTGGCAGGGAAGACTAGCATCTCACCCAACACCACCTACCCTTCCCTTCTGGAGGACTACCGT
  GTGGAATACAGAGTGACTCTCACTGAGGACCAGCTGCCCCACTTAGAGGAGATTCACATCCGGGGACCCG
  TCCGGGATGACATTGAGATTCAGGTGTACAGACGATATGGAGGAGAATATGGGGATCTTACACACCCAGA
  CATCACCTTTTCCTACTTTCAACTGAAGCAGCAGGCAGCCTGGGTATGGACCGCTAAGCGTGGACCCTGC
  TCAGTGAGCTGTGGGGCAGGGCTGCGCTGGGTGACCTACAGCTGCCAGGATCAAGCTCAAGACAAGTGGG
  TAAAGAACGCCCAGTGCCAAGGGAGCCCACAGCCACCTGCATGGCAAGAGCCTTGTGTCTCTGCCCCCTG
  CTCCCCATATTGGGTAGCTGGGGACTTCAGCCCATGTAGCGTGTCTTGTGGCGGGGGCCTTCGGGAGCGG
  TCACTGCGCTGTGTAGAGACCCAAGATGGCTTCTTAAAGACACTGCCACCTGCCCGGTGCAGAGCAGTAG
  CCCAGCAGCCAGCAGCAGAAGTGGAAAACTGCAACTCCCAGCCCTGTCCCACCAGGTGGGAGGTGTCAGA
  CCCTGGCCCTTGCATGTCATCTGCCTGTGAGGCAGGTCTGGACTCAAGGAATGTGACATGTGTGTCCAGG
  GCGGGTGACCCGGAGAAGCCAGAAACTGCAGGCCCCTGCCGCACCGACGAGATGTCAGCTATGCTGGAGC
  CCTGCTCCAGGAGCCTGTGTTCTCCAGGCTTGGGTCAGGTGGACAACACCATGTCTCTGGGAGAGGAGGC
  TCCATCCCCGGTGGGCAGTGACAAGCCAGGGGCTCAGGCTGAGCATGTGTGGACCCCTCTGGTGGGGCTG
  TGCTCCATCTCTTGTGGGAGAGGTCTGAAGGAACTGTATTTCCTGTGCATGGATTCTGTCCTCAAAATGC
  CTGTCCAGGAAGAGCTATGCGGCTTGGCTAGTAAGCCCCCAAGCCGGTGGGAGGTCTGCAGGGCTCGCCC
  CTGTCCTGCTCGGTGGGAGACTCAAGTCTTGGCACCGTGCCCGGTGACCTGTGGTGGGGGGCGAGTGCCA
  CTGTCTGTTCGTTGTGTGCAGCTAGACCGTGGCCACCCGATATCTGTACCTCACTCCAAGTGCTCGCCAG
  TGCCTAAGCCAGGCTCCTTCGAGGACTGCAGCCCTGAGCCTTGTCCTGCTAGGGCACTAGTGTGGGAAGC
  CGCCCCCACATTCGCCGTCACAAGATGGCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231513 representing NM_001290464
  Red=Cloning site Green=Tags(s)
  
  MSQLCLWLTCQPCYAVSVRGILTGAIFILGCWGLSDFQKSLLQDLEPKDVSSYFGHHAAPFTGHPPSHLQ
  RLRRRRTLEDILHLELLVAVGPDVSRAHQEDTERYVLTNLNIGSELLRNPSLGVQFQVHLVKLITLSDSE
  STPNITANITSSLMSVCEWSQTINPHDDRDPSHADLILYITRFDLELPDGNQQVRGVTQLGGACSLSWSC
  LITEDTGFDLGVTIAHEIGHSFGLDHDGAPGSGSTCKASGHVMAADGATPTGGTLEWSACSQRQLQHLLS
  TGQMHCFQDPPGLQSGLTRHQLMAQPGLYYSADDQCRVAFGSGAVACTFSREGLDVCQALSCHTDPLDQS
  SCSRLLVPLLDGTECGVEKWCSKARCRSLAELAPVAAVHGHWSSWGPHSPCSRSCGGGVITRRRWCNNPR
  PAFGGRACVGEDLQAKMCNTQACEKTQLEFMSEQCAQTDRQPLQLSQGTASFYHWDAAVQYSQGDTLCRH
  MCWAVGESFIVSRGDRFLDGTRCVPSGPQDDGTLSLCLLGSCRTFGCDGRMDSQKVWDACQVCGGDNSTC
  SSRNGSFTAGRAREYVTFLIVTPNMTNAHIVNRRPLFTHLAVRIQGHYIVAGKTSISPNTTYPSLLEDYR
  VEYRVTLTEDQLPHLEEIHIRGPVRDDIEIQVYRRYGGEYGDLTHPDITFSYFQLKQQAAWVWTAKRGPC
  SVSCGAGLRWVTYSCQDQAQDKWVKNAQCQGSPQPPAWQEPCVSAPCSPYWVAGDFSPCSVSCGGGLRER
  SLRCVETQDGFLKTLPPARCRAVAQQPAAEVENCNSQPCPTRWEVSDPGPCMSSACEAGLDSRNVTCVSR
  AGDPEKPETAGPCRTDEMSAMLEPCSRSLCSPGLGQVDNTMSLGEEAPSPVGSDKPGAQAEHVWTPLVGL
  CSISCGRGLKELYFLCMDSVLKMPVQEELCGLASKPPSRWEVCRARPCPARWETQVLAPCPVTCGGGRVP
  LSVRCVQLDRGHPISVPHSKCSPVPKPGSFEDCSPEPCPARALVWEAAPTFAVTRWR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290464
• ORF Size: 3111 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290464.1, NP_001277393.1
• RefSeq Size: 3474 bp
• RefSeq ORF: 3114 bp
• Locus ID: 279028
• Cytogenetics: 2 A3
• MW: 113.5 kDa
• Gene Summary: This gene encodes a member of "a disintegrin and metalloproteinase with thrombospondin motifs" (ADAMTS) family of multi-domain matrix-associated metalloendopeptidases that have diverse roles in tissue morphogenesis and pathophysiological remodeling, in inflammation and in vascular biology. In certain mouse strains (C57BL/6, for example) an intracisternal A-type particle (IAP) retrotransposon sequence is located in the intron 23 that causes an alternate splicing event resulting in a shorter transcript variants encoding shorter isoforms. The encoded preproprotein undergoes proteolytic processing to generate an active enzyme that cleaves von Willebrand factor (VWF) in circulating blood. [provided by RefSeq, Jul 2016]