Trim24 (NM_001272064) Mouse Tagged ORF Clone

CAT#: MR231489

Trim24 (myc-DDK-tagged) - Mouse tripartite motif-containing 24 (Trim24), transcript variant 2

Product Images

Circular map for MR231489

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Trim24
• Synonyms: A130082H20Rik; AI447469; D430004I05Rik; Tif; TIF1; TIF1-alpha; Tif1a; TIF1alpha
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231489 representing NM_001272064
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTGGCTGTGGAGAAGGCGGCGGCGGCAGCGGCTCCGGCCGGAGGCCCCGCAGCGGCGGCGCCGA
  GCGGGGAGAATGAGGCCGAGAGCCGGCAGGGCCCGGACTCGGAGAGCGGCGGCGAGGCGTCCCGGCTCAA
  CCTGTTGGACACTTGCGCCGTGTGCCACCAGAACATCCAGAGCCGGGTGCCCAAGCTGCTGCCCTGCCTG
  CACTCGTTCTGCCAGCGCTGTTTGCCCGCGCCGCAGCGCTATCTCATGCTGACGGCGCCCGCGCTGGGCT
  CGGCAGAGACCCCTCCACCCGCTCCCGCCCCCGCCCCCGCCCCGGGCTCCCCGGCCGGTGGTCCTTCGCC
  ATTCGCCACCCAAGTTGGAGTCATTCGATGCCCAGTTTGCAGTCAAGAGTGTGCTGAGAGACACATCATA
  GACAACTTTTTTGTGAAGGACACCACTGAAGTTCCTAGTAGTACAGTAGAAAAGTCTAATCAGGTATGTA
  CAAGCTGTGAAGACAATGCAGAAGCTAATGGGTTTTGTGTAGAGTGTGTTGAATGGCTCTGCAAGACATG
  TATTAGAGCTCACCAGAGGGTGAAGTTCACAAAAGACCACACAGTCAGGCAGAAAGAAGAAGTATCTCCA
  GAGGCAGTTGGGGTGACCAGTCAGCGACCAGTGTTTTGTCCCTTCCATAAAAAGGAGCAGTTGAAACTTT
  ACTGTGAAACATGTGATAAACTGACCTGTCGAGACTGCCAGCTGCTAGAACACAAAGAACACAGGTATCA
  ATTTATAGAAGAAGCTTTTCAGAATCAAAAAGTGATCATAGATACTCTAATCACCAAACTGATGGAAAAA
  ACAAAATATATAAAGTATACAGGAAATCAGATCCAAAATAGGATAATTGAAATAAATCAAAACCAAAAGC
  AGGTGGAACAGGATATTAAAGTTGCCATCTTCACATTGATGGTGGAGATAAACAAAAAAGGGAAAGCTCT
  GCTGCACCAGCTTGAGAGTCTTGCAAAGGACCATCGAATGAAACTCATGCAACAACAGCAGGAAGTGGCT
  GGGCTTTCTAAGCAGTTAGAGCACGTCATGCATTTTTCTAAATGGGCTGTTTCCAGTGGCAGCAGCACAG
  CCTTGCTGTACAGCAAGCGGCTGATTACATACAGGTTACGGCACCTTCTTCGTGCAAGGTGTGATGCTTC
  TCCTGTGACCAACACCACCATCCAGTTTCACTGTGATCCTAGTTTCTGGGCTCAAAATATTATCAACTTG
  GGTTCTTTAGTAATCGAGGATAAAGAGAGCCAGCCACAAATGCCTAAGCAGAATCCTGTCGTGGAGCAGA
  GTTCACAGCCACCAGGTGGTTTACCTTCCAACCAGTTATCCAAGTTCCCAACACAGATCAGCCTAGCTCA
  GTTACGACTCCAGCATATTCAGCAACAGCATCCGCCACCACGCTTAATAAACTTTCAGAATCACAGCCCT
  AAGCCCAATGGACCAGTTCTTCCTCCTTATCCTCAGCAGCTGAGATATTCACCAAGCCAGAATGTACCTC
  GGCAGACAACAATAAAGCCCAACCCCTTGCAAATGGCTTTTTTGGCTCAACAGGCCATAAAACAGTGGCA
  GATCAGCAGTGTACAGGCTCCGCCCACAACTGCCAGCAGCTCCTCCTCCACGCCGTCCAGCCCCACAATC
  ACAAGTGCAGCTGGGTACGATGGAAAAGCTTTTAGTTCACCCATGATTGATCTGAGTGCACCGGTGGGAG
  GGTCTTACAATCTTCCTTCTCTTCCAGATATTGATTGTTCAAGTACTATAATGTTGGACAACATTGCAAG
  GAAAGACACAGGTGTAGATCACGCCCAGCCGAGGCCTCCGTCAAACAGAACGGTGCAGTCACCAAATTCA
  TCAGTGCCATCTCCAGGCCTTGCAGGGCCTGTTACTATGACTAGCGTCCATCCCCCAATACGTTCACCTA
  GTGCCTCCAGTGTTGGAAGTCGAGGAAGCTCTGGCTCTTCCAGCAAACCAGCAGGAGCTGATTCTACTCA
  CAAGGTCCCAGTAGTCATGCTGGAGCCAATTCGAATAAAACAGGAAAACAGTGGACCACCTGAAAATTAT
  GATTTTCCTGTTGTTATAGTAAAACAAGAATCAGATGAAGAATCTAGACCTCAAAATACTAACTATCCAA
  GAAGCATACTTACCTCCCTCCTCTTAAACAGCAGTCAGAGCTCTGCTTCTGAGGAAACCGTGTTACGATC
  TGATGCCCCTGATAGTACAGGAGATCAGCCTGGACTCCATCAAGAAAATTCCTCAAATGGAAAGTCTGAG
  TGGTCGGATGCCTCCCAGAAGTCCCCTGTGCATGTCGGAGAGACGAGGAAGGAGGATGACCCCAATGAAG
  ACTGGTGTGCTGTTTGTCAAAATGGTGGGGAACTCCTATGCTGTGAGAAATGTCCTAAAGTATTCCATCT
  TACTTGTCATGTGCCCACCTTGACAAATTTTCCAAGTGGAGAATGGATCTGTACTTTCTGCCGAGACTTA
  TCTAAGCCAGAGGTTGACTATGATTGTGATGTTCCCAGTCACCACTCAGAGAAACGGAAAAGTGAAGGCC
  TTACTAAGTTAACGCCAATAGACAAAAGGAAATGTGAACGCCTACTTCTGTTTCTTTACTGCCATGAAAT
  GAGCCTGGCTTTCCAAGACCCTGTTCCTCTAACTGTGCCTGATTATTATAAAATAATTAAAAACCCAATG
  GACTTGTCAACCATCAAGAAAAGACTTCAGGAGGATTATTGCATGTATACAAAGCCTGAAGACTTTGTAG
  CTGATTTTAGATTGATCTTTCAAAACTGTGCTGAATTCAATGAGCCTGATTCTGAAGTAGCCAATGCTGG
  TATAAAACTTGAAAGCTATTTTGAAGAACTTCTAAAGAATCTTTATCCAGAAAAAAGGTTTCCTAAGGTA
  GAATTCAGGCATGAAGCAGAAGACTGTAAGTTCAGTGACGACTCAGACGATGACTTTGTACAGCCCCGGA
  AGAAGCGTCTCAAGAGCACCGAGGATCGCCAGCTGCTTAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231489 representing NM_001272064
  Red=Cloning site Green=Tags(s)
  
  MEVAVEKAAAAAAPAGGPAAAAPSGENEAESRQGPDSESGGEASRLNLLDTCAVCHQNIQSRVPKLLPCL
  HSFCQRCLPAPQRYLMLTAPALGSAETPPPAPAPAPAPGSPAGGPSPFATQVGVIRCPVCSQECAERHII
  DNFFVKDTTEVPSSTVEKSNQVCTSCEDNAEANGFCVECVEWLCKTCIRAHQRVKFTKDHTVRQKEEVSP
  EAVGVTSQRPVFCPFHKKEQLKLYCETCDKLTCRDCQLLEHKEHRYQFIEEAFQNQKVIIDTLITKLMEK
  TKYIKYTGNQIQNRIIEINQNQKQVEQDIKVAIFTLMVEINKKGKALLHQLESLAKDHRMKLMQQQQEVA
  GLSKQLEHVMHFSKWAVSSGSSTALLYSKRLITYRLRHLLRARCDASPVTNTTIQFHCDPSFWAQNIINL
  GSLVIEDKESQPQMPKQNPVVEQSSQPPGGLPSNQLSKFPTQISLAQLRLQHIQQQHPPPRLINFQNHSP
  KPNGPVLPPYPQQLRYSPSQNVPRQTTIKPNPLQMAFLAQQAIKQWQISSVQAPPTTASSSSSTPSSPTI
  TSAAGYDGKAFSSPMIDLSAPVGGSYNLPSLPDIDCSSTIMLDNIARKDTGVDHAQPRPPSNRTVQSPNS
  SVPSPGLAGPVTMTSVHPPIRSPSASSVGSRGSSGSSSKPAGADSTHKVPVVMLEPIRIKQENSGPPENY
  DFPVVIVKQESDEESRPQNTNYPRSILTSLLLNSSQSSASEETVLRSDAPDSTGDQPGLHQENSSNGKSE
  WSDASQKSPVHVGETRKEDDPNEDWCAVCQNGGELLCCEKCPKVFHLTCHVPTLTNFPSGEWICTFCRDL
  SKPEVDYDCDVPSHHSEKRKSEGLTKLTPIDKRKCERLLLFLYCHEMSLAFQDPVPLTVPDYYKIIKNPM
  DLSTIKKRLQEDYCMYTKPEDFVADFRLIFQNCAEFNEPDSEVANAGIKLESYFEELLKNLYPEKRFPKV
  EFRHEAEDCKFSDDSDDDFVQPRKKRLKSTEDRQLLK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001272064
• ORF Size: 3051 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001272064.1, NP_001258993.1
• RefSeq Size: 6089 bp
• RefSeq ORF: 3054 bp
• Locus ID: 21848
• UniProt ID: Q64127
• Cytogenetics: 6 B1
• MW: 113.3 kDa
• Gene Summary: The protein encoded by this gene is part of the tripartite-motif containing family (TRIM), which are typified by the RING, B-box type 1, B-box type 2, and coiled-coil region domains. This protein, which also contains a PHD/TTC finger and bromodomain important for regulating nuclear receptors and binding chromatin, has important roles in differentiation, development, and tissue homeostasis. This protein has been reported to regulate the activity of the tumor suppressor p53 and of the retinoic acid receptor. A translocation event between this gene and Braf transforming gene, which results in the fusion protein T18, has been reported in hepatocellular carcinomas. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013]