Nbr1 (NM_001252220) Mouse Tagged ORF Clone

CAT#: MR231462

Nbr1 (myc-DDK-tagged) - Mouse neighbor of Brca1 gene 1 (Nbr1), transcript variant 2

Product Images

Circular map for MR231462

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nbr1
• Synonyms: mKIAA0049
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231462 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACCACAGGTTACTCTAAATGTGACTTTTAAAAATGAAACTCAAAGCTTTCTGGTTTCGGATCCAG
  AAAATACAACCTGGGCTGATGTTGAAGCTATGGTAAAAGTTTCATTTGATCTGAATACTATCCAGATAAA
  ATACCTGGATGAGGAGAACGAGGAGATATCCATCAATAGTCAAGGTGAATATGAAGAAGCACTTAAGATG
  GCTAACATTAAGCAAGGAAATCAGCTACAGATGCAAGTCCACGAAGGGTACCATGTTGTAGATGAAGCAC
  TCCCCAAAAATGTGGTAGAAAACCAAGCGGCTGCCAGGACAGGGAAGAAGCCACTTGCACATTATTCTTC
  ACTGGTGAGAGTCTTGGGATCAGACATGAAGACCACAGAGGAGCCTGCACCAGAGCAATGTTCTTCTGCT
  CCTTGTGACACAGACCAGCCTCAAGACAAGCCCCCAGATTGGTTTACAAGCTACCTGGAGATGTTCCGAG
  AACAAGTGGTTAAGGAAACGGTGGAGAAACTTGAACAGAGGTTGCAGGAGAAGCTTGTCCTCCAGAAGCC
  ACTCCTGAGTTCCTCACCCACAGAAGTCTCAATGCCTATTTCAGAGGAAACCCTGTTTTTGCCAGAGAAC
  CAGTTCAGCTGGCATATTGCTTGTAGCCACTGCCAGAAGAGGATCGTTGGAGTGCGCTACCAGTGTAGCC
  TATGCCCATCCTACAACATTTGTGAAGATTGTGAAGCTGGACCATACACCCATGACACTAACCATGTTTT
  GTTAAAGCTGCGGAGACCTGTTGTGATATCCTCTGAGCCATTTTTCTACTCCAAGTATTCCGCTCCTCGT
  CTGCCTGCTGCTTTGGAACAAGTCAGGCTCCAAAAACAGGTGGACAAGAACTTTGTGAAAGCAGAAAAGC
  AAAGGTTGCGGGCTGAGAAGAAACAGCGGAAGGCAGAGGTCAAAGAGCTTAAAAAGCAGCTTAAACTCCA
  CAGGAAGATTCATTTATGGAATTCAATCCATGGACTTCAGAGTCCCAAGTCCCCTTTGGGCCGACCTGAG
  AGCTTGCTGCAGTCTAACACCCTGATGCTTCCTTTGCAGCCCTGTGCCCCAGTTATGCCAACACTCAGTG
  CAGCATTTGTGGATGAGAATTTGCCTGATGGGACTCATCTTCAGCCAGGAACCAAGTTTATTAAACACTG
  GAGGATGAAAAACACGGGGAATGTGAAGTGGAACACAGACACAAAGCTCAAGTTCATGTGGGGAAACTTG
  ACTCTGGCTTCTACAGAGAAGAAAGATGTTTTGGTTCCCTGCTTGAAGGCTGGCCACGTTGGGGTCGTGT
  CTGTGGAGTTCATCGCTCCAACCTTGGAGGGGACATACACTTCGCACTGGCGTCTCTCTCACAAAGGCCA
  GCAGTTTGGGCCTCGGGTTTGGTGCAGTATCATAGTGGATCCTTTTCCTTCCTCTGAGAGCCCTGATAAT
  GTTGAAGGGGACAGGATCAGCTCAAGCAAAGCTGACGATTTCTCCTGTGAGCAAGAGGAAGCTTTTCTTC
  TAGCTGAAGAAGAGATTCCATTGGGTGAGGTGACAAAGCAGACAGAAGGGACAGGAGCCAGTGCCTCACA
  GAAGACAAGACGCGCTGCCAGTGAAAGGGAGCTGTACATTCCATCTGTGGACCTCCTGACTGCCCAGGAC
  CTGCTGTCCTTTGAGCTGCTGGATATAAACATTGTCCAAGAATTGGAGAGAGTGCCACACAACACCCCTG
  TGGATATGACTCCCTGCATGTCTCCTCTGCCACATGACAGTCCTTTAATAGAGAAGCCAGGCTTGGGGCA
  GATACAGGAAGAGAGTGAAGGGGCGGGATTTAAAGCACCTCCTGATTCCACTGTATCAGCAAAGAGGAAA
  GCTGAGACCCCTGCTTCGGTCGAAGAAACAGAAGAAGACCTGAGTGGGACCCAATTTGTATGTGAGACTG
  TAATTCGATCCCTTACCTTGGATGCTGCTCCAGACCACAACCCTCCTTGCAGGCAGAGGTCCCCACAGAG
  GGAATTACAGCTGTATTCCACCGAGGGACAGCAGCCACTTGTGCTGCCTGGATTCTGCAGAAAGGATTCT
  TCCTTGAAATTTGCCTTGCCTGAAGAAGGACCGCGTGGAGATGAGAGGGAGGAGATTGTCCACATTGTTG
  AGGAAGAAGTCGTTGAGGAAGAGGAGGAAGTCCAAGATGAAGAAGTCCAAAGTCAGTCTTCTGCTTCCTC
  TGAAGATTATATCATCATCCTTCCTGAGTGCTTTGATACCAGCCGCCCCCTGGGGGACTCCATGTACAGC
  TCTGCGCTCTCCCAGCCAGGCCTGGAGCGAGGGGCTGAAGGCGAACCTGGGATCGAGTCTGGGCTGGAAC
  CAACTGAGGCTAGAGAGAGACTCCCAGAGAGGGAGAGCCAGCCACAGGAGCAGAGCATCAGCGACATCCT
  CACAACCTCACAGCCTCTGGACACGGTGCCTCTAGTCCCCGAGGTGGCAGGACTTCCAGCAGCATTGTCC
  AGGAGCGCCCCTTGTGGACAGTGTGAGTCATCAGGAGTGGATTCACCAGGAGTGGATTCACCAGCTACCA
  TGCACGAAGTTCCCCCAGCCCCTGATGACATCAGAGGAGAGCCCAGAGGCTCAACAGGACTCGCAAACAG
  CAGACAGAGGAGCTGTGACCACTCAAGGCACCACAATGGGAGCAGCATTGCTGGAGGACTGGTGAAGGGG
  GCTTTGTCTGTTGCTGCCTCTGCGTACAAGGCCCTGTTTTCTGGGCCACCAGTCACTGCACAGCCCATCG
  TTTCTGAAGATCAGACCACAGCCCTGATGGCCCATCTCTTTGAAATGGGATTCTGCGACAGGCAGCTGAA
  CCTAAGGCTGCTGAGAAAACACAATTACAATATCCTGCAGGTTGTGACAGAGCTCCTTCAAGTGAATAAC
  AACGACTGGTACAGCCACCGCTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231462 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEPQVTLNVTFKNETQSFLVSDPENTTWADVEAMVKVSFDLNTIQIKYLDEENEEISINSQGEYEEALKM
  ANIKQGNQLQMQVHEGYHVVDEALPKNVVENQAAARTGKKPLAHYSSLVRVLGSDMKTTEEPAPEQCSSA
  PCDTDQPQDKPPDWFTSYLEMFREQVVKETVEKLEQRLQEKLVLQKPLLSSSPTEVSMPISEETLFLPEN
  QFSWHIACSHCQKRIVGVRYQCSLCPSYNICEDCEAGPYTHDTNHVLLKLRRPVVISSEPFFYSKYSAPR
  LPAALEQVRLQKQVDKNFVKAEKQRLRAEKKQRKAEVKELKKQLKLHRKIHLWNSIHGLQSPKSPLGRPE
  SLLQSNTLMLPLQPCAPVMPTLSAAFVDENLPDGTHLQPGTKFIKHWRMKNTGNVKWNTDTKLKFMWGNL
  TLASTEKKDVLVPCLKAGHVGVVSVEFIAPTLEGTYTSHWRLSHKGQQFGPRVWCSIIVDPFPSSESPDN
  VEGDRISSSKADDFSCEQEEAFLLAEEEIPLGEVTKQTEGTGASASQKTRRAASERELYIPSVDLLTAQD
  LLSFELLDINIVQELERVPHNTPVDMTPCMSPLPHDSPLIEKPGLGQIQEESEGAGFKAPPDSTVSAKRK
  AETPASVEETEEDLSGTQFVCETVIRSLTLDAAPDHNPPCRQRSPQRELQLYSTEGQQPLVLPGFCRKDS
  SLKFALPEEGPRGDEREEIVHIVEEEVVEEEEEVQDEEVQSQSSASSEDYIIILPECFDTSRPLGDSMYS
  SALSQPGLERGAEGEPGIESGLEPTEARERLPERESQPQEQSISDILTTSQPLDTVPLVPEVAGLPAALS
  RSAPCGQCESSGVDSPGVDSPATMHEVPPAPDDIRGEPRGSTGLANSRQRSCDHSRHHNGSSIAGGLVKG
  ALSVAASAYKALFSGPPVTAQPIVSEDQTTALMAHLFEMGFCDRQLNLRLLRKHNYNILQVVTELLQVNN
  NDWYSHRY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001252220
• ORF Size: 2967 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252220.1, NP_001239149.1
• RefSeq Size: 4866 bp
• RefSeq ORF: 2967 bp
• Locus ID: 17966
• UniProt ID: P97432
• Cytogenetics: 11 65.36 cM
• MW: 110 kDa
• Gene Summary: Acts probably as a receptor for selective autophagosomal degradation of ubiquitinated targets.[UniProtKB/Swiss-Prot Function]