Ephb2 (NM_001290753) Mouse Tagged ORF Clone

CAT#: MR231460

Ephb2 (myc-DDK-tagged) - Mouse Eph receptor B2 (Ephb2), transcript variant 1

Product Images

Circular map for MR231460

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ephb2
• Synonyms: Cek5; Dr; Drt; Er; Erk; ETECK; Hek5; Nu; Nuk; Prk; Prkm5; Qek5; Sek; Sek3; Tyr; Tyro5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231460 representing NM_001290753
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGTGCGCAGGCTGGGGGCCGCGCTGCTGCTGCTGCCGCTGCTAGCCGCCGTGGAAGAAACCCTGA
  TGGACTCTACGACAGCAACGGCTGAGCTGGGCTGGATGGTACATCCCCCATCAGGGTGGGAAGAGGTGAG
  CGGCTACGACGAGAACATGAACACTATCCGTACCTACCAGGTGTGCAATGTCTTTGAGTCAAGCCAGAAC
  AACTGGCTGCGGACCAAATTCATCCGGCGCCGCGGCGCCCACCGCATCCACGTGGAGATGAAGTTCTCGG
  TGCGTGACTGCAGCAGCATTCCCAGCGTGCCGGGCTCCTGCAAGGAGACCTTCAACCTCTACTACTATGA
  GGCTGATTTTGACTTAGCCACCAAAACCTTTCCCAACTGGATGGAGAATCCGTGGGTGAAGGTGGACACC
  ATCGCGGCCGATGAGAGCTTCTCTCAGGTGGACCTGGGTGGCCGCGTCATGAAAATCAACACTGAGGTGC
  GAAGCTTCGGTCCCGTGTCCCGCAATGGTTTCTACCTGGCCTTCCAGGACTATGGCGGCTGTATGTCCCT
  CATTGCTGTGCGCGTCTTCTACCGGAAGTGCCCCCGAATCATCCAGAATGGTGCCATCTTCCAGGAGACA
  CTGTCGGGGGCTGAGAGCACTTCGCTGGTGGCAGCTCGGGGCAGCTGCATCGCCAATGCTGAAGAAGTGG
  ATGTGCCCATCAAACTCTACTGTAACGGGGACGGCGAATGGCTGGTGCCCATAGGTCGCTGCATGTGCAA
  GGCGGGCTTCGAGGCTGTGGAGAACGGCACCGTCTGCCGAGGTTGTCCATCAGGAACCTTCAAGGCCAAC
  CAAGGGGACGAAGCCTGCACCCACTGTCCCATCAACAGCCGCACCACCTCCGAGGGTGCCACCAACTGTG
  TATGCCGCAACGGCTACTACAGGGCCGACCTGGACCCCTTAGACATGCCTTGCACAACCATCCCCTCTGC
  GCCCCAGGCTGTGATCTCCAGCGTCAACGAGACGTCCCTCATGCTAGAGTGGACCCCACCCCGAGACTCG
  GGGGGTCGCGAGGATCTTGTTTACAACATCATCTGCAAGAGCTGTGGCTCCGGCCGGGGCGCATGCACGC
  GCTGCGGGGACAACGTGCAGTACGCGCCCCGCCAGCTGGGCCTGACTGAGCCGCGCATCTACATCAGTGA
  CCTGCTGGCACACACGCAGTACACCTTCGAGATCCAGGCCGTGAACGGTGTGACTGACCAGAGTCCCTTC
  TCACCTCAGTTCGCCTCTGTGAACATCACCACCAACCAAGCAGCACCATCGGCCGTGTCCATCATGCACC
  AGGTGAGCCGCACTGTGGACAGCATCACCCTGTCGTGGTCCCAGCCAGACCAGCCCAACGGTGTGATCCT
  GGACTACGAGCTGCAGTACTATGAGAAGCAGGAGCTCAGTGAGTACAACGCCACGGCCATAAAAAGCCCC
  ACCAACACAGTCACTGTGCAGGGCCTCAAAGCCGGCGCCATCTATGTCTTCCAGGTGCGGGCACGCACCG
  TTGCAGGCTATGGGCGCTACAGTGGCAAGATGTACTTCCAAACCATGACAGAAGCCGAGTACCAGACCAG
  CATCAAGGAAAAGCTACCCCTCATCGTTGGCTCCTCCGCCGCCGGCTTAGTCTTCCTCATCGCTGTGGTC
  GTCATTGCCATCGTATGTAACAGACGGGGGTTTGAGCGTGCCGACTCAGAGTACACGGACAAGCTACAAC
  ACTACACCAGCGGACACATGACCCCAGGCATGAAGATCTATATAGACCCTTTCACCTATGAAGATCCTAA
  TGAGGCAGTGCGGGAGTTTGCCAAGGAAATTGACATCTCCTGTGTCAAGATTGAGCAGGTGATCGGAGCA
  GGGGAATTTGGTGAGGTCTGCAGTGGCCATTTGAAGCTGCCAGGCAAGAGAGAGATCTTTGTAGCCATCA
  AGACCCTCAAGTCAGGATACACGGAGAAACAGCGCCGGGACTTCCTGAGTGAGGCATCCATCATGGGCCA
  GTTCGACCACCCCAATGTCATCCATCTGGAAGGGGTTGTCACCAAGAGCACACCTGTCATGATCATCACT
  GAATTCATGGAGAACGGATCTCTGGACTCCTTCCTCCGGCAAAACGATGGGCAGTTCACAGTCATCCAAC
  TGGTGGGCATGCTGAGGGGCATTGCAGCCGGCATGAAGTACCTGGCGGACATGAACTACGTGCACCGTGA
  CCTTGCTGCTCGAAACATCCTCGTCAACAGCAACCTGGTGTGTAAGGTGTCTGATTTTGGGCTCTCACGC
  TTCCTGGAGGATGACACGTCTGACCCCACCTATACCAGCGCTCTGGGTGGGAAGATCCCCATCCGTTGGA
  CGGCACCGGAAGCCATCCAGTACCGGAAATTCACCTCGGCCAGTGATGTGTGGAGCTATGGCATCGTCAT
  GTGGGAGGTGATGTCCTACGGGGAACGACCCTACTGGGACATGACCAATCAAGACGTAATCAACGCCATT
  GAACAGGACTACAGACTACCTCCGCCCATGGACTGCCCTAGTGCCCTGCACCAGCTCATGCTGGACTGCT
  GGCAGAAGGACCGCAACCACCGGCCCAAGTTCGGCCAGATTGTCAACACGCTGGACAAGATGATCCGAAA
  CCCCAACAGCCTCAAAGCCATGGCACCCCTGTCCTCTGGCATCAACCTGCCACTGCTGGACCGCACGATA
  CCGGACTACACCAGCTTTAACACGGTGGATGAGTGGCTAGAGGCCATCAAGATGGGCCAGTACAAGGAGA
  GCTTTGCCAACGCCGGCTTTACCTCTTTCGACGTTGTATCTCAGATGATGATGGAGGACATTCTCCGCGT
  TGGGGTCACTCTAGCTGGCCACCAGAAAAAAATCCTGAACAGTATCCAGGTGATGCGGGCCCAGATGAAC
  CAGATCCAGTCTGTAGAGGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231460 representing NM_001290753
  Red=Cloning site Green=Tags(s)
  
  MAVRRLGAALLLLPLLAAVEETLMDSTTATAELGWMVHPPSGWEEVSGYDENMNTIRTYQVCNVFESSQN
  NWLRTKFIRRRGAHRIHVEMKFSVRDCSSIPSVPGSCKETFNLYYYEADFDLATKTFPNWMENPWVKVDT
  IAADESFSQVDLGGRVMKINTEVRSFGPVSRNGFYLAFQDYGGCMSLIAVRVFYRKCPRIIQNGAIFQET
  LSGAESTSLVAARGSCIANAEEVDVPIKLYCNGDGEWLVPIGRCMCKAGFEAVENGTVCRGCPSGTFKAN
  QGDEACTHCPINSRTTSEGATNCVCRNGYYRADLDPLDMPCTTIPSAPQAVISSVNETSLMLEWTPPRDS
  GGREDLVYNIICKSCGSGRGACTRCGDNVQYAPRQLGLTEPRIYISDLLAHTQYTFEIQAVNGVTDQSPF
  SPQFASVNITTNQAAPSAVSIMHQVSRTVDSITLSWSQPDQPNGVILDYELQYYEKQELSEYNATAIKSP
  TNTVTVQGLKAGAIYVFQVRARTVAGYGRYSGKMYFQTMTEAEYQTSIKEKLPLIVGSSAAGLVFLIAVV
  VIAIVCNRRGFERADSEYTDKLQHYTSGHMTPGMKIYIDPFTYEDPNEAVREFAKEIDISCVKIEQVIGA
  GEFGEVCSGHLKLPGKREIFVAIKTLKSGYTEKQRRDFLSEASIMGQFDHPNVIHLEGVVTKSTPVMIIT
  EFMENGSLDSFLRQNDGQFTVIQLVGMLRGIAAGMKYLADMNYVHRDLAARNILVNSNLVCKVSDFGLSR
  FLEDDTSDPTYTSALGGKIPIRWTAPEAIQYRKFTSASDVWSYGIVMWEVMSYGERPYWDMTNQDVINAI
  EQDYRLPPPMDCPSALHQLMLDCWQKDRNHRPKFGQIVNTLDKMIRNPNSLKAMAPLSSGINLPLLDRTI
  PDYTSFNTVDEWLEAIKMGQYKESFANAGFTSFDVVSQMMMEDILRVGVTLAGHQKKILNSIQVMRAQMN
  QIQSVEV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290753
• ORF Size: 2961 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290753.2, NP_001277682.1
• RefSeq Size: 4875 bp
• RefSeq ORF: 2964 bp
• Locus ID: 13844
• UniProt ID: P54763
• Cytogenetics: 4 69.0 cM
• MW: 110.5 kDa
• Gene Summary: This gene encodes a member of the Eph receptor family of receptor tyrosine kinase transmembrane glycoproteins. These receptors consist of an N-terminal glycosylated ligand-binding domain, a transmembrane region and an intracellular kinase domain. The encoded receptor preferentially binds membrane-bound ephrin-B ligands and is involved in nervous system and vascular development. This gene is used as a marker of intestinal stem cells. Homozygous knockout mice for this gene exhibit impaired axon guidance and vestibular function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]