Inpp4a (NM_001290797) Mouse Tagged ORF Clone

CAT#: MR231455

Inpp4a (myc-DDK-tagged) - Mouse inositol polyphosphate-4-phosphatase, type I (Inpp4a), transcript variant 3

Product Images

Circular map for MR231455

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Inpp4a
• Synonyms: 107kDa; 9630012D15; D130048C09Rik; R74740
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231455 representing NM_001290797
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGCAAGAGAGCACAGCCCTCGCCATGGTGCCAGGGCCCGTGCGATGCAGCGGGCTTCCACCATTG
  ACGTGGCAGCCGACATGGTTGGCCTCTCTTTGGCAGGAAATATCCAAGATCCAGATGAGCCCATTTTAGA
  ATTCAGCTTAGCTTGCAGTGAGCTGCACACTCCATCGCTAGATCGAAAACCAAATAGTTTTGTGGCTGTG
  AGTGTCACCACCCCTCCGCAGGCGTTCTGGACGAAGCACGCACAGACGGAGATCATCGAGGGAACCAACA
  ACCCTATCTTTCTAAGCAGCATTGCCTTCTTTCAAGACTCCCTTATCAATCAGATGACCCAGATCAAGCT
  GTCTGTGTATGATGTCAAAGACAGATCTCAGGGAACAATGTACTTACTGGGCTCTGGAACATTCGTGGTC
  AAAGATCTGCTCCAGGACAGGCATCACAGATTGCATCTGACACTGAGGTCTGCAGAGAGTGACCGTGTCG
  GCAACATAACTGTGATCGGCTGGCAGATGGAGGAGAAGTCCGACCAGCAGCCCCCTGTGACCCGGTCTCT
  GGACACTGTCAATGGAAGGATGGTTCTGCCCGTTGATGAAAGCTTGACTGAGGCCTTGGGAATCCGATCC
  AAATATGCTTCTTTGCGAAAAGACAGCTTACTGAAAGCAGTGTTTGGTGGTGCCATCTGCCGCATGTACC
  GCTTCCCAACTACTGATGGTAACCACCTACGGATCCTGGAGCAGATGGCAGAGAGCGTCCTCTCCCTGCA
  CGTGCCTCGGCAGTTTGTGAAGCTACTGCTGGAAGAAGATGCAGCCAGGGTATGTGAGTTGGAAGAGTTG
  GGGGAGCTGTCCCCTTGCTGGGAGAGCCTCCGGCGCCAGATTGTCACCCAGTATCAGACTATCATCCTCA
  CCTACCAGGAGAATCTGACTGACCTCCATCAGTACAAAGGTCCTTCCTTTAAAGCAAGCAGTTTGAAAGC
  AGATAAAAAGTTAGAATTTGTTCCCACAAACCTGCACATACAACGGATGCGAGTTCAAGATGATGGAGGC
  TCAGATCAGAACTACGATGTCGTCACTATTGGAGCCCCAGCAGCACACTGCCAAGGTTTTAAGTCAGGAG
  GTCTTCGAAAAAAGCTGCACAAGTTTGAAGAGGCCAAGAAACATTTTGAGGAGTGTTGTACATCTTCTAG
  TGGCCAGTCCATCATCTACATACCACAGGACATCACCCGAGCCAAGGAGATCATTGCCCAGATCAACACC
  CTAAAAACCCAGGTCAGCTACTACGCGGAACGGCTCTCAAGGGCTGCGAAGGACAGGTCAGCTACTGGCC
  TTGAGAGGACTCTCGCCATCTTGGCAGACAAGACTCGGCAGTTGGTGACTGTCTGTGACTGTAAGCTGCT
  GGCCAACTCCATCCATGGGCTGAATGCAGCTCGGCCCGACTACATCGCTTCCAAGGCCTCCCCTACCTCG
  ACTGAGGAGGAGCAGGTGATGCTTCGGAATGACCAGGACACCCTGATGGCCAGGTGGGCGGGGAGAAGCA
  GCCGGTCTTCCCTGCAGGTGGACTGGCATGAGGAGGAGTGGGAGAAAGTGTGGCTGAATGTGGACAAGAG
  CCTGGAGTGCATCATTCAGCGGGTGGACAAGCTGCTGCAGAAGGAGCGTCTGCATGGGGAGGGCTGCGAG
  GATGCTTTCCCCTGTTCGAGCACCTGCTCCAGCAAGAAAGGTAACCGGGACAGCCAAGCCTACTGGATCC
  GACCGGAGGACCCCTTGTGTGACGCCCCCTCCTCGACATGCCCCTCCTCCATGCCCACTGCCGCATGCCA
  TCCTCATTCAAGCACACATTGCAGCCCCCCTCCTGAAGAGTCCAGTCCAGGTGAGTGGAGCGAGGCCCTT
  TACCCTCTGCTGACCACCCTCACAGACTGTGTGGCCATGATGAGCGACAAGGCCAAGGCAGCCATGGTCT
  TCCTGCTCATGCAGGACAGTGCCCCCACCATTGCCTCGTACCTCAGCCTGCAGTATCGCCGTGACGTCGT
  CTTCTGCCAAACCCTGACAGCCCTCATCTGTGGCTTTATCATCAAGCTGAGGAACTGCCTGCACGACGGT
  GGCTTCCTGCGGCAGCTCTACACCATCGGCCTTCTGGCCCAGTTTGAGAGCCTGCTGAGCACCTACGGAG
  AGGAGTTGGCCATGTTGGAGGACATGAGCCTTGGAATCATGGACCTGAGGAATGTGACCTTTAAAGTCAC
  TCAGGCCACTTCGAATGCATCAAATGACATGCTGCCGGTCATCACAGGAAACCGGGATGGCTTTAACGTG
  CGGATCCCTCTGCCAGGCCCACTGTTTGACTCCCTCCCCAGAGAGATCCAGAGTGGCATGCTGCTGCGGG
  TGCAGCCTGTCCTCTTCAACGTGGGCATCAATGAGCAACAGACACTGGCTGAGAGGTTTGGAGATACGTC
  CTTACAAGAAGTTATCAATGTGGAGAGCTTGGTGCGGTTAAATTCCTACTTTGAGCAGTTTAAGGAGGTT
  TTGCCGGAGGACTGTCTACCTCGATCTCGGAGTCAGACCTGCCTTCCAGAGCTGCTGCGGTTCCTGGGAC
  AGAATGTCCATGCACGCAAGAATAAGAATGTGGACATCCTCTGGCAAGCTGCTGAGGTCTGTCGCCGCCT
  TAACGGGGTCCGATTCACCAGCTGCAAGAGTGCCAAGGACCGCACAGCCATGTCGGTGACCCTGGAGCAG
  TGTCTGATCCTGCAGCACGAGCACGGCATGGCCCCGCAGGTCTTCACACAAGCCCTGGAGTGCATGCGCA
  GTGAGGGTTGTCGGCGAGAAAACACAATGAAGAATGTTGGAAGTCGCAAATATGCATTTAACTCCCTGCA
  GCTGAAGGCCTTCCCCAAGCATTACAGGCCTCCAGAAGGGACTTACGGAAAAGTTGAGACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231455 representing NM_001290797
  Red=Cloning site Green=Tags(s)
  
  MTAREHSPRHGARARAMQRASTIDVAADMVGLSLAGNIQDPDEPILEFSLACSELHTPSLDRKPNSFVAV
  SVTTPPQAFWTKHAQTEIIEGTNNPIFLSSIAFFQDSLINQMTQIKLSVYDVKDRSQGTMYLLGSGTFVV
  KDLLQDRHHRLHLTLRSAESDRVGNITVIGWQMEEKSDQQPPVTRSLDTVNGRMVLPVDESLTEALGIRS
  KYASLRKDSLLKAVFGGAICRMYRFPTTDGNHLRILEQMAESVLSLHVPRQFVKLLLEEDAARVCELEEL
  GELSPCWESLRRQIVTQYQTIILTYQENLTDLHQYKGPSFKASSLKADKKLEFVPTNLHIQRMRVQDDGG
  SDQNYDVVTIGAPAAHCQGFKSGGLRKKLHKFEEAKKHFEECCTSSSGQSIIYIPQDITRAKEIIAQINT
  LKTQVSYYAERLSRAAKDRSATGLERTLAILADKTRQLVTVCDCKLLANSIHGLNAARPDYIASKASPTS
  TEEEQVMLRNDQDTLMARWAGRSSRSSLQVDWHEEEWEKVWLNVDKSLECIIQRVDKLLQKERLHGEGCE
  DAFPCSSTCSSKKGNRDSQAYWIRPEDPLCDAPSSTCPSSMPTAACHPHSSTHCSPPPEESSPGEWSEAL
  YPLLTTLTDCVAMMSDKAKAAMVFLLMQDSAPTIASYLSLQYRRDVVFCQTLTALICGFIIKLRNCLHDG
  GFLRQLYTIGLLAQFESLLSTYGEELAMLEDMSLGIMDLRNVTFKVTQATSNASNDMLPVITGNRDGFNV
  RIPLPGPLFDSLPREIQSGMLLRVQPVLFNVGINEQQTLAERFGDTSLQEVINVESLVRLNSYFEQFKEV
  LPEDCLPRSRSQTCLPELLRFLGQNVHARKNKNVDILWQAAEVCRRLNGVRFTSCKSAKDRTAMSVTLEQ
  CLILQHEHGMAPQVFTQALECMRSEGCRRENTMKNVGSRKYAFNSLQLKAFPKHYRPPEGTYGKVET
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290797
• ORF Size: 2931 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290797.1, NP_001277726.1
• RefSeq Size: 5862 bp
• RefSeq ORF: 2934 bp
• Locus ID: 269180
• UniProt ID: Q9EPW0
• Cytogenetics: 1 15.46 cM
• MW: 110.1 kDa
• Gene Summary: Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 1,4-bisphosphate. Involved in the regulation of megakaryocyte and fibroblast proliferation. Regulates cell growth downstream of transcription factor GATA-1.[UniProtKB/Swiss-Prot Function]