Vps54 (NM_001290628) Mouse Tagged ORF Clone

CAT#: MR231439

Vps54 (myc-DDK-tagged) - Mouse vacuolar protein sorting 54 (yeast) (Vps54), transcript variant 2

Product Images

Circular map for MR231439

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Vps54
• Synonyms: Hcc8; mSLP8; Vps54l; wr
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231439 representing NM_001290628
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCAAGCCACAGTTCTTCTCCAGTGCCTCAAGGAAGCAGCAGTGATGTTTTCTTTAAAAAAGAGG
  TAGATCCAACAAAACACATTCGACCTGTGCAGTCACTGCCAGATGTGTGTCCCAAGGAACCCACAGTTAC
  AGATCAACATAGATGGACTGTGTATCATTCCAAAGTAAATCTCCCAGCAGCATTAAATGATCCTACACTA
  GCAAAAAGAGAATCTGACTTCTTCACAAAAACTTGGGGATTGGACTTTGTGGACACTGAAGTCATACCTT
  CACTCTACCTCCCACAGATCAGCAAGGAGAATTTTATAGCATATCAGCAGGAGATCTCTCAGAGAGAGAA
  GATTCATGAGAGATGCAAGAATATTTGTCCTCCTAAAGATACCTTTGACAGGACTCTTTTACATATTCAT
  GATAAATCCAGGACAGATCTGGAGCAAGTACCTAAGATTTTTATGAAACCAGATTTTGCCTTGGATGATT
  CTTTAACTTTTAATTCAGTTTTACCATGGTCTCATTTTAATACTGCTGGTGGGAAAGGAAGTCGCGACGC
  AGCTTCTTCCAAGTTGCTTCAGGAAAAGCTGAGCCATTATCTGGATATTGTGGAAGTAAACATTGCTCAC
  CAGATCTCTCTACGTTCAGAAGCATTTTTTCATGCAATGACCTCTCAACACGAATTGCAGGACTACCTCA
  AGAAAACTACCCAGGCTGTAAAAATGCTTCGAGATAAAATTGCACAGATTGATAAAGTTATGTGTGAAGG
  ATCACTACAGATTTTAAGACTGGCACTTACCAGAAATAATTGTGTTAAAGTATACAATAAACTGAAGTTA
  ATGGCCACTGTACACCAAACTCAGCCTACAGTGCAGGTGTTGTTATCTACTTCTGAATTTGTTGGAGCGT
  TGGACTTAATAGCAACAACTCAAGAGGTTCTGCAGCAGGAGCTTCAGGGCATTCACAGCTTCCGGCATTT
  GGGATCACAGCTTTGTGAATTAGAAAAGCTGATTGATAAAATGATGATTGCAGAATTTTCTACTTATTCT
  CATAGTGACTTAAATAGACCACTGGAAGGTGAATGCCAAGTTTTAGAAGAGGAAAGATTAGTATCTCTTG
  TATTTGGGCTTTTGAAACAAAGAAAACTTAATTTTTTAGAAATCTATGGTGAAGAAATGATTATTACAGC
  AAAGAACATCATTAAAGAGCGTGTGATTAATAAAGTTTCACAAATAGAAGAAATAGACACAGACGTTGTT
  GTAAAGCTTGCAGATCAAATGAGAATGTTGAATTTTCCCCAGTGGATTGATCTGTTAAAGGACATTTTCT
  CTAAGTTTACAGTTTTCCTACAGAGAGTTAAGGCAACATTGAATATTATCCACAGTGTTGTTCTGTCAGT
  TCTTGAGAAAAGCCAAAGGACTAGAGAATTGGAAGAGATCCCACAGCAGAGGAGCGCTGGGAAGGACAGC
  TCCTTGGACACAGACGTGGCTTACCTGACACACGAGGGCTGGTTCATAAGTGATGCTTTCAGTGAGGGTG
  AGCCAGCATCTGCGGCGGTTGATACTACCTCTCAAAGAAACACGTCTCCACATAGCGAGCCCTGCAGCAG
  TGATTCGGTATCAGAACCAGAATGTACTACTGATTCTTCATCCAGCAAGGAGCAGACATCAGCGTGTGCT
  CCTCCAGGAGGTATAGAGATTATAGTCAGTGAAGACATGAGGTTAACTGACTTGGAGCTAGGAAAACTAG
  CAAGTAACATCCAGGAGTTACTGTGTAATGCTTCAGATGTATGCCATGATCGAGCTGTCAAATTTCTCAT
  GTCAAGAGCAAAGGACGGTTTTCTTGAAAAATTGAATTCCACGGAATTCATAGCACTCTCGAGATTAATG
  GAGACATTCATAGTGGACACTGAACAGATCTGTGGGAGGAAGAGCACGTCACTGCTTGGGGCCCTTCAGA
  GCCAAGCAAATAAGTTTGTGAACAGGTTTCATGAAGAGAGGCGGACCAAGCTCAGCCTCCTCTTAGACAA
  CGAGCGTTGGAAGCAGGCTGATGTTCCTGCAGAATTTCAGGATCTTGTTGATTCTATAGCGGATGGAAAG
  ATTGCTTTACCTGAAAAAAAGCCAGTGGTTACAGAAGAAAGGAAGCCAGCTGATGTTCTTGTTGTTGAGG
  GACACCAGTATGCAGTTGTCGGAACCGTCTTGCTGTTAATAAGAATCATTCTTGAATATTGCCAGTGTGT
  GGATAACATCCCCTCCGTCACTACTGACATGCTCACTCGCCTGACAGATCTGCTGAAGTACTTCAATTCA
  AGAAGTTGCCAGTTAGTTCTTGGAGCTGGTGCTTTGCAAGTTGTTGGACTAAAAACAATTACTACAAAAA
  ACTTGGCCCTCTCTTCACGGTGTCTGCAGTTGATTGTGCACTACATTCCCGTGATCCGGGCTCACTTTGA
  AGCCCGACTGCCCCCCAAGCAATGGAGCATGCTTAGACATTTTGATCACATCACAAAGGATTACCATGAT
  CATATAGCTGAAATTTCAGCTAAGCTTGTAGCGATAATGGATAGCCTATTTGATAAGCTGTTATCAAAGT
  ATGAGGTGAAAGCTCCTGTTCCTTCCCCGTGTTTCAGGAATATTTGTAAGCAAATGACAAAAATGCATGA
  AGCTATATTTGATCTTCTTCCCGAAGAACAAACACAGATGTTATTTTTAAGAATTAATGCAAGTTACAAA
  CTCCACTTGAAAAAACAGTTATCACACTTAAATGTAATAAATGACGGAGGACCTCAAAATGGGTTGGTCA
  CAGCAGATGTAGCTTTTTACACTGGAAATCTTCAAGCCTTAAAAGGCCTTAAAGATCTGGATCTAAATAT
  GGCCGAGATCTGGGAACAGAAGAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231439 representing NM_001290628
  Red=Cloning site Green=Tags(s)
  
  MASSHSSSPVPQGSSSDVFFKKEVDPTKHIRPVQSLPDVCPKEPTVTDQHRWTVYHSKVNLPAALNDPTL
  AKRESDFFTKTWGLDFVDTEVIPSLYLPQISKENFIAYQQEISQREKIHERCKNICPPKDTFDRTLLHIH
  DKSRTDLEQVPKIFMKPDFALDDSLTFNSVLPWSHFNTAGGKGSRDAASSKLLQEKLSHYLDIVEVNIAH
  QISLRSEAFFHAMTSQHELQDYLKKTTQAVKMLRDKIAQIDKVMCEGSLQILRLALTRNNCVKVYNKLKL
  MATVHQTQPTVQVLLSTSEFVGALDLIATTQEVLQQELQGIHSFRHLGSQLCELEKLIDKMMIAEFSTYS
  HSDLNRPLEGECQVLEEERLVSLVFGLLKQRKLNFLEIYGEEMIITAKNIIKERVINKVSQIEEIDTDVV
  VKLADQMRMLNFPQWIDLLKDIFSKFTVFLQRVKATLNIIHSVVLSVLEKSQRTRELEEIPQQRSAGKDS
  SLDTDVAYLTHEGWFISDAFSEGEPASAAVDTTSQRNTSPHSEPCSSDSVSEPECTTDSSSSKEQTSACA
  PPGGIEIIVSEDMRLTDLELGKLASNIQELLCNASDVCHDRAVKFLMSRAKDGFLEKLNSTEFIALSRLM
  ETFIVDTEQICGRKSTSLLGALQSQANKFVNRFHEERRTKLSLLLDNERWKQADVPAEFQDLVDSIADGK
  IALPEKKPVVTEERKPADVLVVEGHQYAVVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLTDLLKYFNS
  RSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPVIRAHFEARLPPKQWSMLRHFDHITKDYHD
  HIAEISAKLVAIMDSLFDKLLSKYEVKAPVPSPCFRNICKQMTKMHEAIFDLLPEEQTQMLFLRINASYK
  LHLKKQLSHLNVINDGGPQNGLVTADVAFYTGNLQALKGLKDLDLNMAEIWEQKR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290628
• ORF Size: 2895 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290628.1, NP_001277557.1
• RefSeq Size: 4533 bp
• RefSeq ORF: 2898 bp
• Locus ID: 245944
• UniProt ID: Q5SPW0
• Cytogenetics: 11 13.89 cM
• MW: 109.7 kDa
• Gene Summary: Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD. Within the GARP complex, required to tether the complex to the TGN. Not involved in endocytic recycling.[UniProtKB/Swiss-Prot Function]