Aftph (NM_001290545) Mouse Tagged ORF Clone

CAT#: MR231399

Aftph (myc-DDK-tagged) - Mouse aftiphilin (Aftph), transcript variant 3

Product Images

Circular map for MR231399

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aftph
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231399 representing NM_001290545
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCAGACATCATTCGAATGTACTCTTCCTCCCCCCCACCTCTAGACAACGGAGCAGAGGATGATG
  AGGAGGATGAGTTTGGGGAGTTTGGTGGGTTTTCGGAAGTCAGCCCTTCTGGTGTAGGGTTTGTTGATTT
  TGACACACCAGATTATACTCGTCCCAAGGAAGACTTTGTACCTTCAAACCACTTTATGCCAATTCATGAA
  TATTCAGAAGATGTAGATAGCCTTACAAGCTTTAAGTCTGTTCAGAATGGTAATGATAAGGATATCACTG
  CTGAACTTTCTACTCCTGTGAAAAGTCAATCTGATGTTGTACTTTCTACCACCAGCAAAGAAATGATTCC
  ATCTAAAACTTTGGATCCTTCCATCGATGGTATGGAAAGCCTGGAAGACTTAGATAAAGTAGTGGTGCAG
  GGACCAAGCACTGGGCAACTCAGAAGTTTCTCTCCTGGAGATTTTAGAACTGATAAGAACATTGTTCACC
  AAACCAAGCAGTTAGAGAGCTGCAACGGTGAAAAGCCTCCTTGTCTGGAAATTCTAACAAACGGGTTTGC
  AGGTTTGGAAACTGTAAATCCTCAGGGAACAGATGACCTGGACAATGTGGCAGATTCAAAGGGATCGAAG
  CCTCTTAACACTTGTGGCACCGAGTGCATTTTAGAGTCTGCAGCTAGTCATGCTACGGAATTTGCAGATT
  TTTCTACATTTTCCCAAACAGAAAGGACACAACTGGAAGAAATAGAATGTCCAGTTTTAAATGATGGTGA
  TACACTGACCATTCAGGGAAACAGCAAAGGCCCCAGAGTCAAGGAACTAAACTGTGTGAAAGAAGTGACT
  TTGGATGGAAGCTTTGAGGATACGGGGAACACTGAAAGAGAACATCAGGTCTGCGTCTCAGAAATCCATG
  CCGTAGCTGACAGAGGGCTCAGTGTTGAAAAACAAGACCTTCAAACACTGCAACAGGATGAGTTTCTAAA
  TTCAAGGATTCAGTCTGAGGCTTGGAGCTTGGTAGACTCAAGTGAAAATTCAGAAGCCATTACAAAGGAG
  CGGTGTAAAATGGAAAAAAATGACTTATTTGCCTCTAAGTGTGCTGACTTATCCATGGATTCTGTTAAAA
  CTTCAGACGTTAATGAAATTGGTTCTTCCAAAGAAGAAAACAGAAAGCTGACTAATCCCAAAAGCCCAGA
  CCCTGATCCCACAGGACAAAATGCTTTGGATGATTCTGCTGCAAGCATGAAAAATGGTGACAGTGGGAAT
  GGCTTTGTGACTTGTCATGATACCAATGAGGATGATTTTGGTGACTTTGGTACAGCCAATGGCACCACTC
  CACCCTTTGTTACTAGTACTCAAGATTCAATGAGTGACGTCACTTTTGAAGACTCCTCAGAGCATTTTCT
  ACATCTTAGTGAACCAGGTGATGATTTTGGAGAATTTGAGGATACAAATGCTGTTTCTTGTCAAGAGGAA
  ATGAGATTTACTGAGTCAGACCTAAGACAGACTTCTGATGGCTTATCAGAGGAATGTCCATTGGCAGGAG
  AGTCTGGTGGAAAAGACAGTAAACCTGACTCAAAACTGAAAAATGGTCAAGACAGTGAATTTGGAGATTT
  TGATTCTGTGCCAAATACTCAAGGTAGTGCTTTTCAAGACTCGGATGATTTTGCAGACTTCAGTTCAGCA
  GGTCCTAGCCAAGCTGTAGATTGGAATGCTTTTGAGGATGAACAGAAAGATGGTTGTTCTTGGGCTGCCT
  TTGGGGATCAGCAGGAGACTGAGTCTCATCATCTAAAGGAAGTCTGGCAGTCACAGAGGACAGATGAAAC
  TATGGGCACTCTGGGAACCCCTAAGATGCACAGTGTTTCTTCAGCAGCTTCCAAAGGAGCAGTTGCTAGT
  GGCCATTTGCAGGAACCAGGCACTTCAGTTCAGACAGCTTTACTCAACCGGTTGGAGAGAATTTTCGAAG
  CCTGTTTTCCGTCTGTGTTTGTCCCTGATGTGGAAGAGGAGGTTTCTTCCCTGAAGCACTTGCTGGAGAC
  ACACAGCTCGCCAGCAAAAACAAGAGAGGCCTTAGCTGACCGTGGTGAGTTGAGGGGTGTATGGACCGAA
  CTGCAGGACATCCATGATGCACATGGTTTGCGGTACCAGTGGGGTGGCTCACATAGCAACAAGAAGCTTT
  TGTGCTCCTTGGGAATAGACACTCGAAACATTCTATTCACTGGCAATAAGAAGCAACCTGTTATAGTGCC
  CATGTATGCAGCAGGATTGGGAATGTTAGAGCCCACCAAGGAACCATTAAAACCACTATCTGCTGCAGAA
  AAAATTGCTTCTATTGGTCAGACAACCGTTATGACACCAGAAATAAACACGTGTACATCTGATCCATTCC
  AGGAGTCTCTCCCACCTGTCCAGTTTGACTGGAGTAGCAGTGGCCTTACTAACCCTTTAGATGCTAGTGG
  AGGTTCCACTCTCCTGAACCTTGATTTCTTTGGGCCCGTGGATGACAGTAGCTCTAGCAGCAGCACAATC
  CCAGGTGTGGATCCAGAGTTGTATGAGTTAACAACTGCTAAGCTGGAGACCTCCACCTCAAGCCTCAGAG
  TGACTGATGCATTTGCGAAGCTCATGTCTACAGTGGAAAAGACAAGCACGTCAACCAGGAAACCAAAAAG
  AGAGGAGCACCTGAGTGAGGAAGCCATGAAGGTGATCGCCAGCCTTCCAGACCTGACCTTCATGCATGCC
  AAGGTGTTGATGTTCCCAGCCACTCTGACACCTTCCATGAGCTCCCAAGAACAAGCAGACGCG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231399 representing NM_001290545
  Red=Cloning site Green=Tags(s)
  
  MEPDIIRMYSSSPPPLDNGAEDDEEDEFGEFGGFSEVSPSGVGFVDFDTPDYTRPKEDFVPSNHFMPIHE
  YSEDVDSLTSFKSVQNGNDKDITAELSTPVKSQSDVVLSTTSKEMIPSKTLDPSIDGMESLEDLDKVVVQ
  GPSTGQLRSFSPGDFRTDKNIVHQTKQLESCNGEKPPCLEILTNGFAGLETVNPQGTDDLDNVADSKGSK
  PLNTCGTECILESAASHATEFADFSTFSQTERTQLEEIECPVLNDGDTLTIQGNSKGPRVKELNCVKEVT
  LDGSFEDTGNTEREHQVCVSEIHAVADRGLSVEKQDLQTLQQDEFLNSRIQSEAWSLVDSSENSEAITKE
  RCKMEKNDLFASKCADLSMDSVKTSDVNEIGSSKEENRKLTNPKSPDPDPTGQNALDDSAASMKNGDSGN
  GFVTCHDTNEDDFGDFGTANGTTPPFVTSTQDSMSDVTFEDSSEHFLHLSEPGDDFGEFEDTNAVSCQEE
  MRFTESDLRQTSDGLSEECPLAGESGGKDSKPDSKLKNGQDSEFGDFDSVPNTQGSAFQDSDDFADFSSA
  GPSQAVDWNAFEDEQKDGCSWAAFGDQQETESHHLKEVWQSQRTDETMGTLGTPKMHSVSSAASKGAVAS
  GHLQEPGTSVQTALLNRLERIFEACFPSVFVPDVEEEVSSLKHLLETHSSPAKTREALADRGELRGVWTE
  LQDIHDAHGLRYQWGGSHSNKKLLCSLGIDTRNILFTGNKKQPVIVPMYAAGLGMLEPTKEPLKPLSAAE
  KIASIGQTTVMTPEINTCTSDPFQESLPPVQFDWSSSGLTNPLDASGGSTLLNLDFFGPVDDSSSSSSTI
  PGVDPELYELTTAKLETSTSSLRVTDAFAKLMSTVEKTSTSTRKPKREEHLSEEAMKVIASLPDLTFMHA
  KVLMFPATLTPSMSSQEQADA
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290545
• ORF Size: 2793 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290545.1, NP_001277474.1
• RefSeq Size: 4065 bp
• RefSeq ORF: 2796 bp
• Locus ID: 216549
• UniProt ID: Q80WT5
• Cytogenetics: 11 A3.1
• MW: 101.6 kDa
• Gene Summary: May play a role in membrane trafficking.[UniProtKB/Swiss-Prot Function]