Scml2 (NM_001290652) Mouse Tagged ORF Clone

CAT#: MR231246

Scml2 (myc-DDK-tagged) - Mouse sex comb on midleg-like 2 (Drosophila) (Scml2), transcript variant 2

Product Images

Circular map for MR231246

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Scml2
• Synonyms: 4932420G07Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231246 representing NM_001290652
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCAAACAGCGAATGAAGATCATTTTGACTGGGACAAGTACTTGAAAGAGACTGGATCGATAAGTG
  CTCCTTCTGAATATTTCAGACAGTCTAAGACTCCACCAACTAATGAATTCAAAATTGGTATGAAATTGGA
  AGCCCGTGACCCTCGCAATATTGATTCGGTGTGTGTTGCTTCGGTCATTGGAATTACTGGAGCCAGGTTA
  CGTCTACGACTGGATGGTAGTGACAATAAGAATGATTTTTGGAGACTTGTGGATTCATCAGACATACAAC
  CTGTTGGGACGTGTGAACAAGGAGGAGATTTACTTCAGCCTCCACTGGGGTACACACTGAATACTTCATC
  ATGGCCCATGTTCTTACTACGTGTACTAACTGGATCTGAACTGGCACCTGCAGTGTTCTTTAAGGAGGAA
  CCACCACGTCCACTCCAAAATAATTTCATAGTTGGGATGAAGATTGAAGCTGTCGATAGAAAAAATCCAT
  TTATGATCTGTCCTGCCACAATTGGAGCTGTCTGTGGAGATCAACTTCATATCACTTTTGATGGATGGAG
  TGGAGCATTTGATTATTGGTGTGACTATGACTCCCGAGACATCTTCCCAGTTGGATGGTGTCGCCTCACA
  GGAGATGTATTACAGCCACCAGGAAAAATTGTTGAAAAAAGACCAAGGCGCAAAAGAAGAACCAGACTAT
  GGAGACTTAGAACTGCTCTTTTGGGAAATGAAGAAGAGGCCCCAGAAGCTGCAGAAGAACCTGGGACCAG
  TGTACTTACTTTTGGAGATGAAAACAGAACTTTGAAAGATTGCCGAGGAGAAGCTGCAGAAGAACCTGGG
  ACCAGTGCATTTACTTTTGGAGATGAAAACAGAACTTTGAAAGATTGCCAAGGAGGTTGGAAAAAACCCA
  AGGGCAGAGGATTCATCAAACCTGGAAAAGATGAAACCAGACCTGGGAAACATGACCAAGGAGCCCCAGC
  TGGGAAAAAACCCAGGGGCAGAGGATTCACCCAACCTTTGGAAGATGAAGCCAGACCAGGAAGAGATGTC
  CAAGTAGCCCCAGCTGAGAAAAAACGCAAGGGCAAAACAGTCACCACACCTTGGACTGAGGATCCCAGAC
  TTTTTGCAGATCAAGGAGATGCCCCAGCTGAGAAAAAACGCAAGGGCAAAACAGTCACCACACCTTGGAC
  TGAGGATCCCAGACTTTTTGCAGATCAAGGAGCAGCCCCAGCTGAGAAAAAACGCAAGGGCAAAACAGTC
  ACCACACCTTGGACTGAGGATCCCAGACTTTTTGCAGATCAAGGAGATGCCCCAGCTGAGAAAAAACGCA
  AGGGCAAAACAGTCACCACACCTTGGACTGAGGATCCCAGACTTTTTGCAGATCAAGGAGATGCCCCAGC
  TGAGAAAAAACGCAAGGGCAAAACAGTCACCACACCTTGGACTGAGGATCCCAGACTTTTTGCAGATCAA
  GGAGCAGCCCCAGCTGAGAAAAAACGCAAGGGCAAAACAGTCACCACACCTTGGACTGAGGATCCCAGAC
  TTTTTGCAGATCAAGGAGATGCCCCAGCTGAAAAAAAACCCAAGGGCAAAAGAGTCACCAAATCTCGGAA
  AGACCAAGCCCAGTTTTTAGCTGATGAAGAAGCAATGCCAGCTCTTAGTGTGAGCAGTACAGAGAGAACA
  CCACCTTCTTCCTCTGAACAACCAAAGTCTTCTACCTCTGGGAAAACAAAATCCACCTCTAGAGGGGCTC
  AAACTTCAAGGAAGTCTCCACGGAAAACAAGTGTTGTGCAACCAGTACCAAAGACCAGCAAGAAAGCAGG
  AAAATCTAAGTCTACTGGAAATACTTCATCCCCTAAGAAGGGCATTACTATTAAAATTGTCCTACCAAAG
  AAAAAGGGTGGAAAGTCTGGAAAAAAGGAAAAAAGTATTCCAGTTATTTCTTCTACATCTTCAGCTTCTT
  TAAGTACACTGATGAAAAGTTCTTCATCTAATAAGACTTCTGCGGGGCCATCTAAAATAGTGATGTCTAC
  AGTTTGTGTGTATATAAATAAACATGGAGATTGTGGCCCCTTCCTGGATCCACAGAAGGTTCAGCAGCTA
  CCTAACCACTTCGGTCCAGGCCCTGTGAATGTCATTCTCCAGCGGACTGTGCAGGCCTGTGTCAATTGTG
  CCTTTCAGGCCAAGGATGTGTTTCTATTTCTTAAAACAGATAATAGAGGAGGAGAAATGATAACTGCCTT
  CTTTGATGGGAAAGTTCATACTGTTCAGCTCCCTCCAGTGAATAGTGCATCATTTGCACTTCGCTTTCTT
  GAAAATTTCTGCCAAAGCCTGCAGTGTGATAACTTTTTGAGTAGCCAGCCTTTCAGACGTGAGGCTCAAG
  TTCCTACCCCAGATACAGGCACTGATCAAAGCAAACCAGAAAATGGGGAACCAAAGGAAAAGAGAAGCCT
  CAAACGATTGTCTCTGCATCCTCATCGTTCTGCTCCTGTCTCATCTAAGGTTCCCAGAAAGTCTGGGCAA
  GCGTCTAAAGGAAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231246 representing NM_001290652
  Red=Cloning site Green=Tags(s)
  
  MGQTANEDHFDWDKYLKETGSISAPSEYFRQSKTPPTNEFKIGMKLEARDPRNIDSVCVASVIGITGARL
  RLRLDGSDNKNDFWRLVDSSDIQPVGTCEQGGDLLQPPLGYTLNTSSWPMFLLRVLTGSELAPAVFFKEE
  PPRPLQNNFIVGMKIEAVDRKNPFMICPATIGAVCGDQLHITFDGWSGAFDYWCDYDSRDIFPVGWCRLT
  GDVLQPPGKIVEKRPRRKRRTRLWRLRTALLGNEEEAPEAAEEPGTSVLTFGDENRTLKDCRGEAAEEPG
  TSAFTFGDENRTLKDCQGGWKKPKGRGFIKPGKDETRPGKHDQGAPAGKKPRGRGFTQPLEDEARPGRDV
  QVAPAEKKRKGKTVTTPWTEDPRLFADQGDAPAEKKRKGKTVTTPWTEDPRLFADQGAAPAEKKRKGKTV
  TTPWTEDPRLFADQGDAPAEKKRKGKTVTTPWTEDPRLFADQGDAPAEKKRKGKTVTTPWTEDPRLFADQ
  GAAPAEKKRKGKTVTTPWTEDPRLFADQGDAPAEKKPKGKRVTKSRKDQAQFLADEEAMPALSVSSTERT
  PPSSSEQPKSSTSGKTKSTSRGAQTSRKSPRKTSVVQPVPKTSKKAGKSKSTGNTSSPKKGITIKIVLPK
  KKGGKSGKKEKSIPVISSTSSASLSTLMKSSSSNKTSAGPSKIVMSTVCVYINKHGDCGPFLDPQKVQQL
  PNHFGPGPVNVILQRTVQACVNCAFQAKDVFLFLKTDNRGGEMITAFFDGKVHTVQLPPVNSASFALRFL
  ENFCQSLQCDNFLSSQPFRREAQVPTPDTGTDQSKPENGEPKEKRSLKRLSLHPHRSAPVSSKVPRKSGQ
  ASKGN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290652
• ORF Size: 2535 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290652.1, NP_001277581.1
• RefSeq Size: 4701 bp
• RefSeq ORF: 2538 bp
• Locus ID: 107815
• Cytogenetics: X F4
• MW: 93.3 kDa