Cux1 (NM_001291240) Mouse Tagged ORF Clone

CAT#: MR231092

Cux1 (myc-DDK-tagged) - Mouse cut-like homeobox 1 (Cux1), transcript variant 2

Product Images

Circular map for MR231092

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cux1
• Synonyms: CDP; Cutl1; Cux; Cux-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231092 representing NM_001291240
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGCGCGTCAGGTCCGCTCCGAGCTGCCTTCTCCAGCCCGGTTCTCGAAAGCAAAGCCCCGGGGG
  CCCCGCTGGACTGTAAGGAGACTGATTGTGGTTCCTCGTCGCCTCGAGGGGATGGTATCGGGGCTTCCCA
  ATCCGCGGCGGAGCTGCCTGCGTGCCCAGAGGATGCTATTCTGCCTGCCACCCCTCATCGGAACCAGCTG
  CTCGCCCTCGGGGTCCTCCCTGCTAGTGCTAGCTCCGCGTTCGCGGCGCCTGGACAGCCCCAGGATTCTG
  CCAGGTGGATGTTGTGCGTAGCCGGAGCCAAGTTGAAGAGAGAACTTGATGCCACCGCAACAGTATTGGC
  AAACAGGCAAGATGAGAGCGAACAGTCCAGAAAGCGGCTCATTGAGCAGAGCCGAGAATTCAAGAAGAAC
  ACTCCAGAGGATTTACGCAAGCAGGTAGCACCACTGCTAAAGAGCTTCCAAGGGGAGATTGATGCACTGA
  GTAAAAGAAGCAAAGAAGCAGAGGCAGCCTTCTTGACTGTGTACAAGAGACTAATTGATGTTCCAGATCC
  GGTACCAGCCCTGGACGTCGGGCAACAGCTGGAAATAAAAGTGCAGCGTCTACACGACATTGAAACAGAG
  AACCAGAAACTTAGGGAAACACTAGAAGAGTACAACAAGGAGTTTGCTGAAGTGAAAAATCAAGAGGTTA
  CGATAAAAGCACTTAAGGAGAAAATCCGAGAATACGAGCAGACCCTGAAGAGTCAGGCCGAGACCATTGC
  TCTGGAGAAAGAGCAGAAGCTACAAAATGATTTTGCAGAGAAGGAGAGAAAGCTGCAAGAGACACAGATG
  TCCACCACCTCAAAACTGGAGGAAGCTGAGCACAAACTCCAGACTCTGCAAACAGCCCTGGAAAAAACTC
  GAACAGAATTATTTGACCTGAAAACCAAATATGATGAAGAAACTACTGCAAAGGCCGATGAGATCGAGAT
  GATCATGACCGACCTTGAACGAGCCAACCAGAGGGCAGAGGTGGCACAGAGAGAAGCAGAGACTTTAAGG
  GAACAGCTCTCATCGGCCAACCACTCTCTCCAACTGGCCTCGCAGATCCAGAAGGCTCCAGATGTGGAGC
  AGGCCATAGAGGTGCTGACCCGATCCAGCCTAGAAGTAGAGTTGGCTGCCAAAGAGCGGGAGATCGCCCA
  GCTGGTGGAAGATGTGCAGCGACTCCAGGCCAGCCTCACCAAGCTACGTGAGAATTCCGCCAGCCAGATC
  TCACAGCTGGAGCAGCAACTGAATGCCAAGAATAGCACACTCAAACAACTGGAAGAAAAACTCAAAGGCC
  AGGCTGACTATGAAGAAGTGAAGAAAGAGCTGAACACCCTGAAGTCCATGGAGTTTGCACCATCGGAGGG
  AGCAGGGACACAGGACTCTACCAAGCCCCTGGAGGTTTTACTCCTGGAGAAGAACCGCTCGCTGCAGTCC
  GAGAATGCCACGCTGCGCATCTCCAACAGTGACCTGAGCGGGCGCTGTGCGGAGCTGCAGATCCACCTCA
  CTGAGGCCACAGCCAAGGCTGTTGAGCAGAAGGAGCTGATCGCTCGCTTGGAGCAGGACCTCAGCACCAT
  CCAGTCCATCCAACGGCCTGATGCCGAGGGAGCTTCCGAGCAAGGCCTAGAGAAGATTCCAGAACCCATC
  AAGGAAGCTACAGCTCTGTTCTATGGACCCTCAATGTCATCCAGTGGGACCCTTCCAGAAGGCCAGGTGG
  ACTCCCTGCTTTCCATCATCTCCAGCCAAAGGGAACGTTTCCGCACCCGGAACCAAGAGCTGGAAGCCGA
  GAGCCGCATGGCCCAGCACACCATCCAGGCCCTGCAGAGCGAGCTGGACAGCCTGCGCGCTGACAACATC
  AAACTCTTTGAGAAGATCAAGTTCCTGCAGAGTTACCCTGGCAGAGGTATCGGCAGTGACGACACGGAGC
  TGCGATACTCCTCCCAATACGAGGAACGCCTGGACCCTTTCTCCTCCTTCAGCAAGAGGGAGCGGCAGAG
  GAAGTACCTGGGCCTGAGCCCCTGGGACAAGGCCACACTTGGCATGGGCCGTCTGATTCTCTCCAACAAG
  ATGGCCCGCACCATCAGCTTCTTTTACACCTTGTTCCTGCACTGCCTGGTCTTTCTGGTGCTGTACAAGC
  TGGCATGGAGTGAGAGTGTGGAGAGAGACTGTGCTGCCACCTGCGCCAAGAAGTTCGCCGATCATCTGCA
  CAAATTCCACGAGAGTGACAACGGAGCAGCAGCTGGTGACTTATGGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231092 representing NM_001291240
  Red=Cloning site Green=Tags(s)
  
  MERASGPLRAAFSSPVLESKAPGAPLDCKETDCGSSSPRGDGIGASQSAAELPACPEDAILPATPHRNQL
  LALGVLPASASSAFAAPGQPQDSARWMLCVAGAKLKRELDATATVLANRQDESEQSRKRLIEQSREFKKN
  TPEDLRKQVAPLLKSFQGEIDALSKRSKEAEAAFLTVYKRLIDVPDPVPALDVGQQLEIKVQRLHDIETE
  NQKLRETLEEYNKEFAEVKNQEVTIKALKEKIREYEQTLKSQAETIALEKEQKLQNDFAEKERKLQETQM
  STTSKLEEAEHKLQTLQTALEKTRTELFDLKTKYDEETTAKADEIEMIMTDLERANQRAEVAQREAETLR
  EQLSSANHSLQLASQIQKAPDVEQAIEVLTRSSLEVELAAKEREIAQLVEDVQRLQASLTKLRENSASQI
  SQLEQQLNAKNSTLKQLEEKLKGQADYEEVKKELNTLKSMEFAPSEGAGTQDSTKPLEVLLLEKNRSLQS
  ENATLRISNSDLSGRCAELQIHLTEATAKAVEQKELIARLEQDLSTIQSIQRPDAEGASEQGLEKIPEPI
  KEATALFYGPSMSSSGTLPEGQVDSLLSIISSQRERFRTRNQELEAESRMAQHTIQALQSELDSLRADNI
  KLFEKIKFLQSYPGRGIGSDDTELRYSSQYEERLDPFSSFSKRERQRKYLGLSPWDKATLGMGRLILSNK
  MARTISFFYTLFLHCLVFLVLYKLAWSESVERDCAATCAKKFADHLHKFHESDNGAAAGDLWQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001291240
• ORF Size: 2289 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291240.1, NP_001278169.1
• RefSeq Size: 3369 bp
• RefSeq ORF: 2292 bp
• Locus ID: 13047
• Cytogenetics: 5 75.96 cM
• MW: 86 kDa
• Gene Summary: Transcription factor involved in the control of neuronal differentiation in the brain. Regulates dendrite development and branching, and dendritic spine formation in cortical layers II-III (PubMed:20510857). Also involved in the control of synaptogenesis (Probable). In addition, it has probably a broad role in mammalian development as a repressor of developmentally regulated gene expression. May act by preventing binding of positively-activing CCAAT factors to promoters. Component of nf-munr repressor; binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. Represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Binds to the TH enhancer; may require the basic helix-loop-helix protein TCF4 as a coactivator.[UniProtKB/Swiss-Prot Function]