Zfp994 (NM_001205095) Mouse Tagged ORF Clone

CAT#: MR230812

Gm4944 (myc-DDK-tagged) - Mouse predicted gene 4944 (Gm4944)

Product Images

Circular map for MR230812

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zfp994
• Synonyms: EG240038; Gm4944
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230812 representing NM_001205095
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGCTTCTCTCCTAAATGCTCCTCAGGGTCAGTTAACGTTCAAGGATGTGGCTGTGGACTTCTCAC
  AGGAGGAATGGCAATGTCTTGACTGTGCTCAGCAGGCATTGTACATGGATGTGATGTTGGAGAATTACAA
  TAATCTGTTTTTTGTGGAGAATCATTGCATGTGTCCTAAATTTGAGAAGATGTTGGATCAAGACCTACAG
  CATATTGTCCTCGAACATGTAAATACCCAAGTGAAATCTTATGAATGTCATGAGCTTGACAAGATCATCC
  ATGAGTTCACCGGAAGTACACCTTATAAAACTAACCTCAGGGCTACATCTGTTGAATCATCAAACCTAAA
  CAGACATGAAACTGAAAACAACAGAGAACCTTGCAAATATGAAAACCATGTAAACTGTTTCAATTTGTGC
  TCTACCATTAGTCTAAATCAAGGAATCAACATAGGAAAGAAAGAATACAATGGTGCAAAGCTTGATAAGG
  TTTTTGACTCTAAAGAAATCCTCATGTTGAAACAAACCAAAAGTGTAAAGAAACCTTACAAATGTAGTGA
  ATGTGCCAAATGTTTTGCCAAGAAATGCTGGTTTAGAAAACATCAGACAACTCATACAGGAGAAAAATTT
  TACAAATGTAATGAATGTGACAAATCCTTTACCCAAAAAGGCAGTCTTAGTATTCATCAGAAGTATCATA
  CAGGAAAGAAACCTTACAAATGTAGTGAATGTGAGAAATGCTTCATTCAAGTTGGTGATCTTAGAAGACA
  TCAGAGAATTCATACAGGAGAGAAACCTTACAAATGTAATGAATGTGAAAAATGCTTTAGCTACAAAAGC
  AGCCTTAGAACTCATCAGAGAATTCATACAGGAGAGAAGCCTTACAAATGTAGTGAATGTGACAAATGCT
  TTTCCCACAAAGGCAGTCTTAGTATTCATCTGAGAATTCATACAGGAGAGAAACCTTACAAATGTAGTGA
  ATGTGACAAAAGCTTTACCCAAAAAGGCAGTCTTAGTATTCATCAGAGAATTCATGCAGGAGAGAAACCT
  TACAGATGTAGTGAATGTGACAAAGGCTTTACTCTAAAATGCAGTCTTATCATTCATCAAATAATTCATA
  CAGGAGAGAAAGCTTACAAATGTAGTGAATGTGACAAATGCTTTTCCCTCAAAAGTGATCTTAGTATTCA
  TCAAAGAATTCATACAGGAGAAAAACTTTATAGATGTAGTGAGTGTGATAAATGCTTTACAAAGAAATAT
  CGTCTTAGAAGTCATCAGACAATTCATACAGGAGAGAAACCTTACAAGTGCAGTGAATGTGACAAATCTT
  TTACCCTGAAAGGCCATCTTAGAATTCATCAACGAATTCATACAGGAGAGAAACCTTACAAATGTAATGA
  ATGTGACAAATGCTTTACTCAAAAATGCAGTCTTATTATTCATCACAGAATTCATGCCGGAGAAAAACCC
  TACAAATGTAGTGAATGTGACAAATACTACACTCGAAAAAGCCATCTTATTGTTCATCAGCGCATTCACA
  TAGGAGAAAAACCTTACAAATGCAGTGAATGTAACAAATGCTTTACCAACAAATATTGTCTTAGAACTCA
  TCAGAGAATACATTCAGCTAATAAGCCTTACAAATGTAGTGAATGTGACAAATCTTTTGCCCTGAATGCC
  AGGCTCAGGTGTCATCGGATTATTCATACAGGAGAAAAACCATACAAATGTAGTAAATGTGGCAAATACT
  TTACCCAAAAAGGAAGTCTTATAATTCATCAGAGAATTCATACAGGAGAGAAACCTTACAAATGTAGTGA
  ATGTGACAAGTACTTTACCCGAAAAAGCAGTCTTAATGTGCACCAGAGAGTTCATACAGGAGAGAAACCT
  TACAAATGTAGTGAATGTGACAAATGCTTTTCCCATAAAGGTGATCTTAGAAAACATCAGAGAATTCATA
  CAGGAGAAAGAACCTTACAGATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230812 representing NM_001205095
  Red=Cloning site Green=Tags(s)
  
  MNASLLNAPQGQLTFKDVAVDFSQEEWQCLDCAQQALYMDVMLENYNNLFFVENHCMCPKFEKMLDQDLQ
  HIVLEHVNTQVKSYECHELDKIIHEFTGSTPYKTNLRATSVESSNLNRHETENNREPCKYENHVNCFNLC
  STISLNQGINIGKKEYNGAKLDKVFDSKEILMLKQTKSVKKPYKCSECAKCFAKKCWFRKHQTTHTGEKF
  YKCNECDKSFTQKGSLSIHQKYHTGKKPYKCSECEKCFIQVGDLRRHQRIHTGEKPYKCNECEKCFSYKS
  SLRTHQRIHTGEKPYKCSECDKCFSHKGSLSIHLRIHTGEKPYKCSECDKSFTQKGSLSIHQRIHAGEKP
  YRCSECDKGFTLKCSLIIHQIIHTGEKAYKCSECDKCFSLKSDLSIHQRIHTGEKLYRCSECDKCFTKKY
  RLRSHQTIHTGEKPYKCSECDKSFTLKGHLRIHQRIHTGEKPYKCNECDKCFTQKCSLIIHHRIHAGEKP
  YKCSECDKYYTRKSHLIVHQRIHIGEKPYKCSECNKCFTNKYCLRTHQRIHSANKPYKCSECDKSFALNA
  RLRCHRIIHTGEKPYKCSKCGKYFTQKGSLIIHQRIHTGEKPYKCSECDKYFTRKSSLNVHQRVHTGEKP
  YKCSECDKCFSHKGDLRKHQRIHTGERTLQI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001205095
• ORF Size: 1983 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001205095.1, NP_001192024.1
• RefSeq Size: 4880 bp
• RefSeq ORF: 1986 bp
• Locus ID: 240038
• Cytogenetics: 17 A3.3
• MW: 77.9 kDa