Lrrc43 (NM_001289821) Mouse Tagged ORF Clone

CAT#: MR230810

Lrrc43 (myc-DDK-tagged) - Mouse leucine rich repeat containing 43 (Lrrc43), transcript variant 2

Product Images

Circular map for MR230810

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lrrc43
• Synonyms: Gm1061
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230810 representing NM_001289821
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACCAGCGAGTCCAGCACTAGCGACTACAGACAGACCGAGGGCGAGGGCGAGGGTGTACCGGGGA
  CCTTGAGCACGGCTGTGTGTGAGCACCTGCGGAAGTTGTGTCTGCGCGAATTCCCGTGTGGCATCGGGAG
  CTGGAATAAGTCACGCTTTCTTCCTCAAAAATGTCGAGTCTGGAGGGAATTGGTACCCAAGGAGGAGGAG
  ACACTGGTTCCCGAGGAGGAGACTGTGGAGGCCCTGCTGGGCCTGGTGCGCAGCAACCACTCTCCTTGGG
  CTATGCTGAAGGATGCCAGCGCGGAGGACCGTTTCCTGAGGGAGCTGGCCATCCAGAATCCACTGATGAT
  CAAAGACACTTTCTTCTACTCCTACTTCCGGTCCCTGCGGGTGGTGAACAAGGGGGTGAGTCTGGTGGAT
  AAAGACCTCTTGAAATTTCTAAAGCTGGAGGAGTTGGTCCTGAGTGCCAATAAAATTGAGGAAATCGATG
  CCAACAATCTGCCCCCGACATTGAAGGTGCTGGAGCTCTATGGCAATTTGATCGCCAGCATGGAGTGTCT
  GTGCTCAGCCCCGCCCCCAAGGCTGCAGCACCTGGGGCTAGGCCACAACAAATTGCTGGGCCCTTTGGAG
  AGTCTGTATGTCACCTCCCACAACTGGCCCCAACTCGTCTCCCTGGACCTGGGCTTCAACAACTTGACGG
  ACCTGCAGAACATGATCTTGGGACTCAGTACCCTGAGGCACCTGAGGCTCCTAGTACTGCAGGGGAACCC
  ACTATCTCTGGTGCCTTACTACCGTGGCTTTACCATAGACAGCCTGGCACATCTTTGTGTGCTGGACGAC
  ATCACCGTGTCTCCCAATGAGAAGCACCAGTTCCGGGGACTCAACATTCACGGTGACCTTTTGGCACGTG
  AGGCCCAGTTTGTGGTGACGATTGGAAATGTCAGAGGGGTTCTGGACAGCTCTATCTTGGACCCGGAGCC
  GGGACCTGACGGCCCTTTCATTAGCTACAGCTATTATGTGACCTATGACTTTGTGGAAGATGAGGACATG
  GAAAGAAATGTTAGTGGGCTAGTAGAGGCAACACATCATGACAGCGTTCTGGATGAGATCGACAAGCATT
  TCTCTGGCACCGATGAGGAGGACCAACAGGAGGATCCCTTAGACGGCCGCCACCGCCACAGAGGCCGTCA
  GAGGTTTCACCCAGGCTCGACGGAGGAGATGTCCAAGGAGCTGTCTGAGTTCATAGCGAAGGAGATGAGT
  CAGATGGCAGAAGGCTCGGTTGAATCTGGGATAACAGAGGTCGACTGGTCGGAGACGAGCATTTCCATCC
  ACTCTGCGCCCCTGCCACAGTCAATCGACTCTTCTGAAGAGCTGGCCAAGCTGCGGCCAAAGATAGACAT
  CCAGCTCTGTCCATCCCCAGGGACAGTCCTCTTCAACACGGTGCACAAGCCCTGGTCAGATGTTATTCCC
  TGCACCTATGAGATGAAGCACACCCTCAAGGAGCTGATCCGGGTCAAGGCCTTCCTGCTGGCTGGGACTA
  CTATCCTCTCCTGGCCTGTGGTACCCACTCCCGTTGAGAGTCCCTTGCCTGCCAAGAAGGGGAAAGACAA
  CAATAAAAAGAAAGAGCCTGCTAAGGATAAGGTCCACAAGAAGAAAAAGGAGCCGCCCAGGGAGCTGCGG
  CAGGACCCCCCTGTGCTGACGGTGTTGGGCAGCGGGCTGGTGTACCTGGAGCCCTTGCTGGCCGGGGAGG
  CAGTGGTATCCACTGTGTGTAATTTCGGGGTGGTCCGAACCCTGGAGACAGACAGGCTCACACATGCCAG
  GGATTCAAAGAAGGTAAAGAAATCTCTCAAGAAAGATAGGTCGAAAACGGTGCCTCCAACCATGGAAAGC
  GGGTACCAGCCAGAGCCACTGTCGGTGGAGGTCCAGATCCAGCTGCACCAGTACCGCTCGGTGGAGGAAG
  CATTCCTTAGCTTGATTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230810 representing NM_001289821
  Red=Cloning site Green=Tags(s)
  
  METSESSTSDYRQTEGEGEGVPGTLSTAVCEHLRKLCLREFPCGIGSWNKSRFLPQKCRVWRELVPKEEE
  TLVPEEETVEALLGLVRSNHSPWAMLKDASAEDRFLRELAIQNPLMIKDTFFYSYFRSLRVVNKGVSLVD
  KDLLKFLKLEELVLSANKIEEIDANNLPPTLKVLELYGNLIASMECLCSAPPPRLQHLGLGHNKLLGPLE
  SLYVTSHNWPQLVSLDLGFNNLTDLQNMILGLSTLRHLRLLVLQGNPLSLVPYYRGFTIDSLAHLCVLDD
  ITVSPNEKHQFRGLNIHGDLLAREAQFVVTIGNVRGVLDSSILDPEPGPDGPFISYSYYVTYDFVEDEDM
  ERNVSGLVEATHHDSVLDEIDKHFSGTDEEDQQEDPLDGRHRHRGRQRFHPGSTEEMSKELSEFIAKEMS
  QMAEGSVESGITEVDWSETSISIHSAPLPQSIDSSEELAKLRPKIDIQLCPSPGTVLFNTVHKPWSDVIP
  CTYEMKHTLKELIRVKAFLLAGTTILSWPVVPTPVESPLPAKKGKDNNKKKEPAKDKVHKKKKEPPRELR
  QDPPVLTVLGSGLVYLEPLLAGEAVVSTVCNFGVVRTLETDRLTHARDSKKVKKSLKKDRSKTVPPTMES
  GYQPEPLSVEVQIQLHQYRSVEEAFLSLID
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289821
• ORF Size: 1980 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289821.1, NP_001276750.1
• RefSeq Size: 2025 bp
• RefSeq ORF: 1983 bp
• Locus ID: 381741
• UniProt ID: Q3V0L5
• Cytogenetics: 5 F
• MW: 74.6 kDa