Ewsr1 (NM_001283061) Mouse Tagged ORF Clone

CAT#: MR230803

Ewsr1 (myc-DDK-tagged) - Mouse Ewing sarcoma breakpoint region 1 (Ewsr1), transcript variant 1

Product Images

Circular map for MR230803

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ewsr1
• Synonyms: Ews; Ewsh
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230803 representing NM_001283061
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCACGGATTACAGTACCTATAGTCAAGCTGCAGCCCAGCAGGGCTACAGTGCTTACACCGCCC
  AGCCAACTCAAGGATATGCACAGACCACCCAGGCATATGGGCAACAAAGCTATGGAACCTATGGACAGCC
  TACTGATGTCAGCTATACTCAGGCTCAGACCACTGCCACCTACGGGCAGACTGCATATGCAACTTCTTAC
  GGACAGCCTCCCACTGGTTATAGCACTCCAACTGCCCCGCAGGCGTACAGCCAGCCTGTGCAGGGATATG
  GCACTGGGACTTATGACAGCACCACTGCTACAGTCACCACAACGCAGGCCTCTTACGCAGCTCAGACTGC
  ATATGGCACCCAGCCTGCCTACCCCACCTATGGCCAGCAGCCAACAGCCACCGCACCTACCAGACCACAG
  GATGGTAACAAGCCTGCTGAGACTAGTCAACCTCAATCTAGCACAGGGGGTTATAACCAACCCAGCCTAG
  GATATGGACAGAGTAACTACAGCTATCCCCAGGTACCTGGGAGCTACCCAATGCAGCCAGTCACCGCACC
  TCCATCTTATCCTCCTACCAGCTACTCCTCTTCACAGCCGACTAGTTACGATCAGAGCAGTTACTCTCAG
  CAGAACACCTATGGGCAGCCGAGCAGCTATGGACAACAGAGTAGCTATGGTCAACAAAGCAGCTATGGGC
  AGCAGCCTCCTACTAGTTACCCCCCTCAGACTGGATCCTACAGCCAGGCTCCAAGTCAATATAGCCAACA
  GAGCAGCAGCTACGGGCAGCAGAGTTCATTCCGACAGGACCACCCCAGTAGCATGGGTGTTTATGGGCAG
  GAGTCTGGAGGATTTTCCGGACCAGGAGAGAACCGGAGCTTGAGTGGCCCTGATAACCGGGGCAGGGGAA
  GAGGGGGATTTGATCGTGGAGGCATGAGCAGAGGTGGGCGGGGAGGAGGACGCGGTGGACTGGGCAGCGC
  TGGAGAGCGAGGTGGCTTCAATAAGCCTGGTGGACCCATGGATGAAGGACCAGATCTTGATCTAGGCCTT
  CCTATAGATCCCGATGAAGACTCTGACAACAGTGCAATTTATGTGCAAGGATTAAATGACAATGTGACTC
  TGGATGATCTGGCAGACTTCTTTAAGCAGTGTGGGGTTGTCAAGATGAACAAGAGAACTGGACAACCCAT
  GATCCATATCTACCTGGATAAGGAGACAGGAAAGCCTAAAGGGGACGCCACAGTGTCCTATGAAGATCCA
  CCAACTGCAAAGGCTGCCGTGGAATGGTTTGATGGGAAAGATTTTCAAGGAAGCAAACTTAAAGTGTCTC
  TTGCCCGAAAGAAGCCTCCAATGAACAGCATGCGGGGAGGCATGCCACCTCGTGAGGGCAGGGGGATGCC
  ACCACCACTTCGTGGAGGTCCTGGTGGCCCAGGAGGCCCTGGAGGACCCATGGGTCGCATGGGAGGCCGT
  GGAGGAGACAGAGGGGGCTTCCCTCCAAGAGGGCCCCGAGGCTCCAGAGGAAACCCCTCTGGAGGAGGAA
  ATGTCCAGCACCGAGCTGGAGACTGGCAGTGTCCCAATCCGGGCTGTGGAAACCAGAACTTCGCTTGGAG
  AACAGAATGCAACCAGTGTAAGGCCCCTAAGCCCGAGGGCTTCCTCCCGCCACCCTTTCCACCTCCGGGT
  GGTGATCGTGGACGAGGTGGCCCTGGTGGCATGCGAGGAGGAAGAGGAGGACTCATGGACCGTGGTGGTC
  CTGGAGGAATGTTCAGAGGTGGCAGAGGTGGAGACAGAGGAGGCTTCCGAGGTGGCCGTGGAATGGACCG
  AGGTGGCTTTGGTGGAGGAAGACGAGGTGGTCCTGGGGGGCCTCCTGGACCTTTAATGGAACAGATGGGA
  GGAAGAAGAGGCGGACGTGGAGGACCTGGGAAAATGGATAAAGGCGAGCACCGTCAGGAACGCAGAGACC
  GGCCCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230803 representing NM_001283061
  Red=Cloning site Green=Tags(s)
  
  MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSY
  GQPPTGYSTPTAPQAYSQPVQGYGTGTYDSTTATVTTTQASYAAQTAYGTQPAYPTYGQQPTATAPTRPQ
  DGNKPAETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYPMQPVTAPPSYPPTSYSSSQPTSYDQSSYSQ
  QNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSSFRQDHPSSMGVYGQ
  ESGGFSGPGENRSLSGPDNRGRGRGGFDRGGMSRGGRGGGRGGLGSAGERGGFNKPGGPMDEGPDLDLGL
  PIDPDEDSDNSAIYVQGLNDNVTLDDLADFFKQCGVVKMNKRTGQPMIHIYLDKETGKPKGDATVSYEDP
  PTAKAAVEWFDGKDFQGSKLKVSLARKKPPMNSMRGGMPPREGRGMPPPLRGGPGGPGGPGGPMGRMGGR
  GGDRGGFPPRGPRGSRGNPSGGGNVQHRAGDWQCPNPGCGNQNFAWRTECNQCKAPKPEGFLPPPFPPPG
  GDRGRGGPGGMRGGRGGLMDRGGPGGMFRGGRGGDRGGFRGGRGMDRGGFGGGRRGGPGGPPGPLMEQMG
  GRRGGRGGPGKMDKGEHRQERRDRPY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001283061
• ORF Size: 1968 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001283061.2
• RefSeq Size: 2431 bp
• RefSeq ORF: 1971 bp
• Locus ID: 14030
• Cytogenetics: 11 A1
• MW: 69 kDa
• Gene Summary: Might function as a transcriptional repressor.[UniProtKB/Swiss-Prot Function]