Gatad2a (NM_001286450) Mouse Tagged ORF Clone

CAT#: MR230739

Gatad2a (myc-DDK-tagged) - Mouse GATA zinc finger domain containing 2A (Gatad2a), transcript variant 4

Product Images

Circular map for MR230739

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gatad2a
• Synonyms: 1110066C11Rik; BC031407; C80248
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230739 representing NM_001286450
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGAAGAAGCATGCCGAACCCGCAGTCAGAAGCGCACCCTTGAACCTGACCTAACGGAGGATGATG
  TGGAGAACAAGAAAATGAAAATGGAGAAAGGGTCATCAGAGCTGACAGTGGACGGAGACAGCAGGGTGAT
  GCCAGAGCCGAGTGCAGGCTCAGCCCAGGGGTTGCTGAGGACAACAGAGGCCATGGGCACAGGATCTGGC
  GAGGGGCTGTTAGGAGACGGGCCTGTGGACATGCGCACTTCACACAGTGACATGAAGTCTGAGAAGAGAC
  CTCCCTCGCCTGATGTGATTGTGCTCTCTGACAGTGAGCAGCCATCGAGCCCAAGGGTGAATGGCCTGAC
  CACAGTAGCCTTGAAAGACACCAGCACTGAGGCGCTCTTGAAAAGCAGCCCAGAAGAACGAGAGCGGATG
  ATCAAGCAGCTGAAGGAGGAGCTGAGGCTAGAGGAAGCAAAACTGGTCTTGTTGAAGAAGCTGCGGCAGA
  GTCAGATACAGAAGGAAGCCACCGCACAGAAGCCTACAGCTTCCTCAGGGAGCACTGTGACCACCCCTCC
  CCCACTCGTGCGGGGTACCCAGAACATTCCTGCTGGCAAGACCTCACTTCAGACCTCCTCCACTCGAATA
  CCCGGCAGCATCATCCCACCACCACTGGTCCGAGGTGGGCAGCAGGTGTCTGCCAAACTGGGACCACAGG
  CCAGCTCTCAAGTAGTCATGCCACCGCTTGTCAGGGGGGCCCAGGTGAGTCAGATTCACAACATCAGACA
  GCATTCCTCTACGGGGCCACCACCCCTCCTCCTGGCCCCCCGTGCCTCAGTGCCCAGCATGCAGATTCAG
  GGACAGAGGATCATCCAGCAGGGACTTATCCGTGTCGCCAATGTCCCCAACACCAGTCTGCTTGTCAACA
  TCCCTCAGCCTACTGCAGCCTCCATGAAGGGGACAGCAGTTGCTTCTGCTCAGGCAAATTCCACTCCCAC
  CAGTGTGGCTTCTGTGGTTGCATCTGCTGAGTCTCCAGCTAGCCGACAGGCGGCTGCCAAGCTAGCACTG
  CGCAAGCAGCTGGAGAAGACACTGCTGGAGATCCCCCCACCCAAGCCCCCTGCCCCAGAGATGAACTTCT
  TGCCCAGTGCTGCCAATAATGAGTTTATCTACCTGGTCGGCCTGGAGGAGGTGGTGCAGAACCTGCTGGA
  GACACAAGCAGGCAGGATTTCTGCCACTGCAGCAGCTGCTGTGTTATCCCGGGAGCCCTACATGTGTGTG
  CAGTGTAAGACAGACTTCACATGCCGCTGGCGAGAGAAGGGTGGAGCCGTTATGTGTGAGAACTGCATGA
  CATCCAACCAAAAGAAGGCACTCAAGGTGGAGCACACCAGTCGCCTGAAGGCCGCCTTTGTGAAGGCTCT
  ACAGCAAGAGCAGGAGATGGAGCAGCGCCTGCTGCAGCAGGGTGTGGGTACTGCCAGCATCAAGGCTGAG
  CCTGCAGCTCCACATCCCACGCTGAAGCAGGTCATAAAGCCCCGACGCTTCCAGCCAGCTGTCCCGAGGC
  TCAGCCACAGCACCTCGTGGTGTTCTGCACACTTTCAGCCAGTCACCCAAACTGCAGAATGCAGCCTCAG
  CTACGGCCCTGGTAAGCAGGACTGGCAGACATTCCGAGAGAGTCGTGGGCACCGGCAAGGGCACCGCCTC
  CAACTGGAAAAAGACGCCTCTGAGCACAGGTGGGACCCTCGCCTTCGTCAGCCCAAGCTTGGCAGTACAC
  AAGACCTCCTCGGCTGTGGATCGACAGCGAGAATACCTCCTGGACATGATTCCTCCACGCTCCATCCCCC
  AATCAGCCACATGGAAATAGTGGGAGCCGGCCCAGCCGAGGACAGGCCCTCTTCTCTCCCAAGGCCCTTG
  GTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230739 representing NM_001286450
  Red=Cloning site Green=Tags(s)
  
  MSEEACRTRSQKRTLEPDLTEDDVENKKMKMEKGSSELTVDGDSRVMPEPSAGSAQGLLRTTEAMGTGSG
  EGLLGDGPVDMRTSHSDMKSEKRPPSPDVIVLSDSEQPSSPRVNGLTTVALKDTSTEALLKSSPEERERM
  IKQLKEELRLEEAKLVLLKKLRQSQIQKEATAQKPTASSGSTVTTPPPLVRGTQNIPAGKTSLQTSSTRI
  PGSIIPPPLVRGGQQVSAKLGPQASSQVVMPPLVRGAQVSQIHNIRQHSSTGPPPLLLAPRASVPSMQIQ
  GQRIIQQGLIRVANVPNTSLLVNIPQPTAASMKGTAVASAQANSTPTSVASVVASAESPASRQAAAKLAL
  RKQLEKTLLEIPPPKPPAPEMNFLPSAANNEFIYLVGLEEVVQNLLETQAGRISATAAAAVLSREPYMCV
  QCKTDFTCRWREKGGAVMCENCMTSNQKKALKVEHTSRLKAAFVKALQQEQEMEQRLLQQGVGTASIKAE
  PAAPHPTLKQVIKPRRFQPAVPRLSHSTSWCSAHFQPVTQTAECSLSYGPGKQDWQTFRESRGHRQGHRL
  QLEKDASEHRWDPRLRQPKLGSTQDLLGCGSTARIPPGHDSSTLHPPISHMEIVGAGPAEDRPSSLPRPL
  V
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286450
• ORF Size: 1893 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286450.1, NP_001273379.1
• RefSeq Size: 5160 bp
• RefSeq ORF: 1896 bp
• Locus ID: 234366
• Cytogenetics: 8 B3.3
• MW: 68.6 kDa
• Gene Summary: Transcriptional repressor (By similarity). Enhances MBD2-mediated repression. Efficient repression requires the presence of GATAD2B (By similarity).[UniProtKB/Swiss-Prot Function]