Slc7a3 (NM_001301840) Mouse Tagged ORF Clone

CAT#: MR230693

Slc7a3 (myc-DDK-tagged) - Mouse solute carrier family 7 (cationic amino acid transporter, y+ system), member 3 (Slc7a3), transcript variant 2

Product Images

Circular map for MR230693

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc7a3
• Synonyms: Atr; Atrc3; Cat; CAT-3; CAT3; SLC; SLC7A1; SLC7A2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230693 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTGGCAGGCACTTCGAAGATTTGGTCAAAAGCTGGTGCGGAGACGTGTGTTGGAGCTGGGTATGG
  GTGAGACCCGCCTTGCCAGATGCCTGAGCACCCTCGACTTAGTGGCTCTGGGTGTGGGCAGCACATTAGG
  TGCAGGTGTGTATGTCCTGGCTGGAGAGGTGGCCAAAGATAAAGCAGGACCATCCATTGTGATCTGCTTT
  TTGGTGGCTGCTCTGTCCTCTGTGTTGGCTGGACTGTGTTATGCTGAATTTGGTGCCCGGGTCCCCGGCT
  CTGGTTCTGCATATCTCTACAGTTATGTCACCGTAGGTGAACTCTGGGCCTTCACCACCGGCTGGAATCT
  CATTCTCTCCTACGTCATTGGTACAGCCAGTGTGGCCCGGGCTTGGAGCTCTGCTTTTGACAACCTGATT
  GGAAACCACATCTCTCGGACTCTGAAGGGGACCATCTTGCTGAAGATGCCTCATGTTCTTGCAGAATATC
  CAGATTTCTTTGCCTTGGCCCTTGTGTTGCTGCTCACTGGATTGCTGGTTTTAGGGGCAAGTAAATCAGC
  CCTGGTTACCAAAGTGTTCACAGGGATGAACCTTTTGGTTCTCAGCTTTGTCATCATCTCTGGCTTCATT
  AAGGGAGAACTGAGAAACTGGAAGCTCACGAAAGAGGACTATTGCTTGACCATGAGTGAATCCAACGGCA
  CTTGTAGCTTGGACTCTATGGGCTCTGGAGGTTTCATGCCTTTTGGCTTGGAAGGGATTCTCCGTGGCGC
  TGCTACCTGTTTCTATGCCTTCGTTGGTTTTGACTGTATTGCAACCACCGGAGAAGAGGCTCAGAATCCC
  CAGCGCTCCATCCCCATGGGTATTGTGATCTCGATGTTCATCTGCTTTTTGGCTTACTTTGGTGTGTCTT
  CAGCACTTACCCTCATGATGCCTTACTACAAGCTTCATCCTGAGAGCCCTCTGCCTGAAGCATTTTCCTA
  TGTTGGCTGGGAGCCTGCCCGCTACTTGGTGGCTATTGGCTCCCTCTGTGCACTTTCTACTAGCCTTTTG
  GGCTCTATGTTTCCTATGCCTCGGGTGATGTATTCAATGGCAGAAGATGGCCTCCTGTTCCGTGTCCTTG
  CTAAGGTTCACAGTGTCACACATATACCCATTGTGGCCACCTTGGTATCTGGTGTTATTGCAGCATTCAT
  GGCATTCCTCTTTGAACTCACTGACCTCGTGGACCTCATGTCAATCGGGACTCTGCTTGCTCACTCCCTG
  GTGTCCATTTGTGTTCTCATCCTCAGATATCAGCCTGATCAGGAAATGAAGAGTGTTGAAGAGGAAATGG
  AATTGCAAGAGGAGACACTTGAAGCAGAGAAGCTGACTGTCCAGGCTCTATTTTGTCCTGTCAACTCCAT
  CCCCACACTCCTTTCTGGCCGAGTTGTCTATGTTTGCTCCTCACTGCTTGCCGTACTACTAACTGTTCTT
  TGCCTGGTGCTGACCTGGTGGACAACTCCACTTCGTTCTGGAGACCCAGTGTGGGTCACAGTGGTTGTGC
  TGATCCTGGGGCTCATTCTTGCAATTTCTGGGGTCATCTGGAGACAGCCACAGAACCGCACTCCCCTTCA
  CTTTAAGGTGCCTGCTGTTCCTCTACTCCCACTGGTGAGCATCTTTGTGAATGTTTACCTGATGATGCAA
  ATGACAGCTGGCACTTGGGCCCGATTTGGGATCTGGATGCTGATTGGATTTGCTATCTACTTTGGCTATG
  GGATCCAACACAGTATGAAAGAAGTTAAGAATCATCAAACTCTACCCAAAACAAGGGCCCAAACTATAGA
  CCTTGATCTCACAACTTCCTGTGTTCACAGCATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230693 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLWQALRRFGQKLVRRRVLELGMGETRLARCLSTLDLVALGVGSTLGAGVYVLAGEVAKDKAGPSIVICF
  LVAALSSVLAGLCYAEFGARVPGSGSAYLYSYVTVGELWAFTTGWNLILSYVIGTASVARAWSSAFDNLI
  GNHISRTLKGTILLKMPHVLAEYPDFFALALVLLLTGLLVLGASKSALVTKVFTGMNLLVLSFVIISGFI
  KGELRNWKLTKEDYCLTMSESNGTCSLDSMGSGGFMPFGLEGILRGAATCFYAFVGFDCIATTGEEAQNP
  QRSIPMGIVISMFICFLAYFGVSSALTLMMPYYKLHPESPLPEAFSYVGWEPARYLVAIGSLCALSTSLL
  GSMFPMPRVMYSMAEDGLLFRVLAKVHSVTHIPIVATLVSGVIAAFMAFLFELTDLVDLMSIGTLLAHSL
  VSICVLILRYQPDQEMKSVEEEMELQEETLEAEKLTVQALFCPVNSIPTLLSGRVVYVCSSLLAVLLTVL
  CLVLTWWTTPLRSGDPVWVTVVVLILGLILAISGVIWRQPQNRTPLHFKVPAVPLLPLVSIFVNVYLMMQ
  MTAGTWARFGIWMLIGFAIYFGYGIQHSMKEVKNHQTLPKTRAQTIDLDLTTSCVHSI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301840
• ORF Size: 1857 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301840.1, NP_001288769.1
• RefSeq Size: 2277 bp
• RefSeq ORF: 1857 bp
• Locus ID: 11989
• UniProt ID: P70423
• Cytogenetics: X C3- D
• MW: 67.5 kDa
• Gene Summary: The protein encoded by this gene is a member of the system y+ cationic amino acid transporter family. Proteins of this family allow uptake of arginine from extracellular media. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]