Rap1gds1 (NM_001286759) Mouse Tagged ORF Clone

CAT#: MR230661

Rap1gds1 (myc-DDK-tagged) - Mouse RAP1, GTP-GDP dissociation stimulator 1 (Rap1gds1), transcript variant 3

Product Images

Circular map for MR230661

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rap1gds1
• Synonyms: BC011279; GDS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230661 representing NM_001286759
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATAACCTCAGTGACACTTTAAAGAAGCTGAAGATAACAGCTGCTGACAGGACAGAGGGCAGTCTGG
  AAGGATGCTTGGATTGTCTGCTTCAAGCCTTGGCTCAGAATAATGCAGAAACAAGTGAAAAAATACAAGG
  AAGTGGAATTCTTCAGTTGTTTGCAAATCTGTTGACGCCACAGGCCTCCTGCACAGCCAAAGTAGCCGAC
  ATCATAGCAGAAGTAGCCAAAAATGAATTTATGCGAATCCCATGTGTAGATGCTGGATTGATTTCACCAC
  TGGTACAGCTGTTAAATAGCAAAGACCAGGAAGTGCTGCTTCAAACGGGCAGGGCATTAGGAAACATATG
  CTACGATAGCCATGAGGGCAGAAGTGCAGTTGACCAAGCAGGTGGTGCACAGATTGTAATTGACCATTTA
  AGGTCGCTGTGCGGTAGGACAGATCCCGCCAGTGAGAAGCTCATGACTGTCTTTTGTGGCATGCTGATGA
  ACTATAGCAATGAGAATGATTCCCTTCAGGCCCAGCTTATTAGCATGGGTGTTATCCCGACCTTAGTGAA
  GCTACTGGGCATTCACTGCCACAATGCAGCACTCACCGAGATGTGTCTCGTTGCGTTCGGGAATTTAGCA
  GAACTTGAGTCAAGTAAAGAACAGTTTGCTAGCACAAACATTGCTGAAGAGCTGGTAAAACTATTCAAGA
  AACAAATAGAACACGATAAAAGAGAGATGATTTTTGAAGTTCTAGCGCCATTGGCAGAGAATGATGCTAT
  TAAGCTGCAGCTGGTTGAAGCAGGTTTGGTAGAGTGTCTCCTAGAGATCGTCCAACAGAAGGTGGATAGC
  AACAAAGAGGACGACGTTGCCGAGCTTAAAACTGCTTCTGACCTCATGGTTTTATTACTGCTTGGAGATG
  AGTCCATGCAGAAACTTTTTGAAGGAGGAAAAGGCAGTGTGTTCCAAAGGGTGCTCTCCTGGATCCCATC
  GAATAACCACCAGCTGCAGCTGGCTGGGGCATTAGCCATTGCAAATTTTGCCAGGAACGATGGAAATTGT
  ATTCATATGGTAGACAATGGGATTGTAGAAAAACTCATGGATTTGCTGGACAGACACGTAGAAGACGGGA
  ACGTGACGGTGCAGCACGCCGCACTAAGTGCCCTCAGGAATCTAGCCATCCCAGTTGTAAATAAAGCAAA
  GATGTTATCAGCTGGTGTCACGGAGACGGTTTTGAAATTCCTTAAATCTGAAATGCCCCCAGTTCAGTTC
  AAACTCCTGGGAACGCTAAGAATGTTAATAGATGCACAAGAAGCTGCTGAGCAACTGGGAAAGAATGCTA
  AACTGGTAGAGCGTTTGGTTGAATGGTGTGAAGCCAAAGATCATGCCGGCGTGATGGGCGAATCAAACAG
  ACTGCTCTCTGCCCTCATCCGACACAGTAAATCAAAAGATGTAATTAAAACCATTGTACAGAGTGGTGGC
  ATCAAGCACCTGGTTACCATGGCAACTAGTGAACATGTAATCATGCAGAATGAAGCTCTTGTCGCCCTGG
  CACTGATAGCAGCCTTGGAACTGGGTCCCGCTGAGAAGGATCTCGCAAGTGCTCAGCTTGTTCAGATCTT
  ACACCGACTGCTAGCAGATGAGAGAAGTGCCCCGGAGATCAAGTACAATTCCATGGTCCTGATCTGCGCT
  CTCATGGGATCCGAATCTCTATACAAGGAAGTACAGGATTTGGCCTTTCTAGATGTTGTCTCCAAACTTC
  GAAGTCATGAGAACAAAAGTGTTGCCCAACAGGCCTCGCTTACAGAGCAGAGACTTACAGTGGAAAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230661 representing NM_001286759
  Red=Cloning site Green=Tags(s)
  
  MDNLSDTLKKLKITAADRTEGSLEGCLDCLLQALAQNNAETSEKIQGSGILQLFANLLTPQASCTAKVAD
  IIAEVAKNEFMRIPCVDAGLISPLVQLLNSKDQEVLLQTGRALGNICYDSHEGRSAVDQAGGAQIVIDHL
  RSLCGRTDPASEKLMTVFCGMLMNYSNENDSLQAQLISMGVIPTLVKLLGIHCHNAALTEMCLVAFGNLA
  ELESSKEQFASTNIAEELVKLFKKQIEHDKREMIFEVLAPLAENDAIKLQLVEAGLVECLLEIVQQKVDS
  NKEDDVAELKTASDLMVLLLLGDESMQKLFEGGKGSVFQRVLSWIPSNNHQLQLAGALAIANFARNDGNC
  IHMVDNGIVEKLMDLLDRHVEDGNVTVQHAALSALRNLAIPVVNKAKMLSAGVTETVLKFLKSEMPPVQF
  KLLGTLRMLIDAQEAAEQLGKNAKLVERLVEWCEAKDHAGVMGESNRLLSALIRHSKSKDVIKTIVQSGG
  IKHLVTMATSEHVIMQNEALVALALIAALELGPAEKDLASAQLVQILHRLLADERSAPEIKYNSMVLICA
  LMGSESLYKEVQDLAFLDVVSKLRSHENKSVAQQASLTEQRLTVES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286759
• ORF Size: 1818 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286759.1, NP_001273688.1
• RefSeq Size: 3671 bp
• RefSeq ORF: 1821 bp
• Locus ID: 229877
• UniProt ID: E9Q6Q4
• Cytogenetics: 3 H1
• MW: 66.5 kDa
• Gene Summary: Stimulates GDP/GTP exchange reaction of a group of small GTP-binding proteins (G proteins) including Rap1a/Rap1b, RhoA, RhoB and KRas, by stimulating the dissociation of GDP from and the subsequent binding of GTP to each small G protein. Able to promote the Ca(2+) release from the endoplasmic reticulum via both inositol trisphosphate (Ins3P) and ryanodine sensitive receptors leading to a enhanced mitochondrial Ca(2+) uptake.[UniProtKB/Swiss-Prot Function]