Ankib1 (NM_001289529) Mouse Tagged ORF Clone

CAT#: MR230638

Ankib1 (myc-DDK-tagged) - Mouse ankyrin repeat and IBR domain containing 1 (Ankib1), transcript variant 4

Product Images

Circular map for MR230638

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ankib1
• Synonyms: 2310061P20Rik; 4631416I11Rik; AW494740; C80642; mKIAA1386
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230638 representing NM_001289529
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCCAGAAGAACTCGTTGGAGTCAGCGAAGCCTATGAAGATGCTGCCAATTGTCTGTGGCTGTTAA
  CTAACTCCAAGCCTTGTGCGAACTGTAAGTCTCCAATACAGAAGAACGAAGGCTGTAATCACATGCAGTG
  TGCCAAGTGCAAGTATGACTTTTGCTGGATTTGCCTAGAAGAATGGAAAAAACATAGTTCTTCCACTGGA
  GGTTACTACAGATGCACTCGCTATGAAGTCATCCAACATGTGGAGGAGCAATCCAAGGAGATGACAGTGG
  AGGCTGAGAAGAAACATAAACGGTTTCAGGAACTTGACAGATTTATGCACTATTATACAAGATATAAAAA
  CCATGAGCATAGTTATCAGTTAGAACAACGCCTTCTTAAAACAGCCAAAGAAAAGATGGAGCAGTTGAGT
  AGAGCTCTCAAAGAAACTGAAGGAGGCTGTCCAGATACCACTTTCATCGAAGATGCAGTGCATGTTCTTC
  TAAAAACTCGGCGCATCCTCAAGTGTTCTTATCCATATGGATTTTTCTTAGAACCTAAAAGCACAAAGAA
  AGAAATTTTTGAACTAATGCAAACAGACCTGGAAATGGTCACTGAAGACCTTGCCCAGAAAGTCAATAGG
  CCTTATCTTCGTACACCTCGGCACAAGATCATCAGGGCAGCATGCCTTGTGCAGCAGAAAAGGCAAGAGT
  TCCTGGCATCTGTCGCTCGTGGTGTTGCTCCTGCAGACTCACCAGATGCTCCCAGGCGCAGCTTTGCCGG
  TGGAACATGGGATTGGGAATATTTAGGATTTGCTTCACCTGAGGAATATGCTGAATTTCAGTATCGGAGG
  AGACACAGACAGCAGCGCCGTCGAGGAGATGTGCATAGCCTGCTCAGTAACCCTACAGACCTGGATGAAC
  CAAGCGAAAGCACTTTCGATCTCCCAGAGGGGAGCAGCGGCCGCAGGCCTGGTGCATCTGTGGTCAGCTC
  TGCATCGATGAGTGTGCTGCACAGCTCTTCCCTGCGAGACTACAGCCCTGCCAGTCGCTCTGCAAACCAG
  GACTCTCTTCAGGCTCTGAGTTCCTTGGATGAAGATGATCCCAACATACTCCTGGCCATCCAGTTGTCAC
  TGCAAGAGTCTGGGCTGGACATGGATGAAGAGACCAGAGACTTCCTTAGTAACGAGACATCCCTAGGAGC
  CATAGGCTCATCTTTACCTTCCCGACTGGACTCTGTGCCCCGCAGCACTGAGTCTCCTCGGGCTGCGCTG
  AGCAGCTCGGAGCTGCTGGAGCTTGGTGACAGTCTCATGAGACTAGGAGCAGACAGTGACCCATTTTCAA
  CTGACACTTTGAGTTCACGCCCTCTCAGTGAGACAAGAAGTGATTTCTGTCCCTCTTCCAGTGACCTTGA
  CTCAGCTGGCCAGGACCCCAGTGCCAATGACAATCTTCTTGGCAATATTATGGCTTGGTTTCATGACATG
  AACCCGCAGAGTATCGCCCTGATTCCTCCAGCAGCAACCACAGAAATTAGTGCTGAGCCTCAGCTTCCTT
  GTATAAGAGATGGGTCTGAAGGTGTGAGGGACATGGAACTCGTGCCACCAGAAGACTCAGTGTCCAAAGA
  TACTGGTGTTCATGAAGGTGAAAGAGCCCAGATGGAAGAAAATCCTCTGGAAGAGAATATTCTGGCCAGG
  GAAGAGTTGTCTCAAGCTGGTGACAGTAGTAACGAGGCAGTGGGCAGGGGGGACAGGCCAGATGCTGCCA
  GTCAAACCCCTCAGACCTCAAGCGACTGGCTCGAGCAAGTACATTCAGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230638 representing NM_001289529
  Red=Cloning site Green=Tags(s)
  
  MKPEELVGVSEAYEDAANCLWLLTNSKPCANCKSPIQKNEGCNHMQCAKCKYDFCWICLEEWKKHSSSTG
  GYYRCTRYEVIQHVEEQSKEMTVEAEKKHKRFQELDRFMHYYTRYKNHEHSYQLEQRLLKTAKEKMEQLS
  RALKETEGGCPDTTFIEDAVHVLLKTRRILKCSYPYGFFLEPKSTKKEIFELMQTDLEMVTEDLAQKVNR
  PYLRTPRHKIIRAACLVQQKRQEFLASVARGVAPADSPDAPRRSFAGGTWDWEYLGFASPEEYAEFQYRR
  RHRQQRRRGDVHSLLSNPTDLDEPSESTFDLPEGSSGRRPGASVVSSASMSVLHSSSLRDYSPASRSANQ
  DSLQALSSLDEDDPNILLAIQLSLQESGLDMDEETRDFLSNETSLGAIGSSLPSRLDSVPRSTESPRAAL
  SSSELLELGDSLMRLGADSDPFSTDTLSSRPLSETRSDFCPSSSDLDSAGQDPSANDNLLGNIMAWFHDM
  NPQSIALIPPAATTEISAEPQLPCIRDGSEGVRDMELVPPEDSVSKDTGVHEGERAQMEENPLEENILAR
  EELSQAGDSSNEAVGRGDRPDAASQTPQTSSDWLEQVHSV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289529
• ORF Size: 1800 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289529.1, NP_001276458.1
• RefSeq Size: 6253 bp
• RefSeq ORF: 1803 bp
• Locus ID: 70797
• UniProt ID: Q6ZPS6
• Cytogenetics: 5 A1
• MW: 67.7 kDa
• Gene Summary: Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates.[UniProtKB/Swiss-Prot Function]