Ankib1 (NM_001289529) Mouse Untagged Clone

CAT#: MC228427

Ankib1 (untagged) - Mouse ankyrin repeat and IBR domain containing 1 (Ankib1), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ankib1
• Synonyms: 2310061P20Rik; 4631416I11Rik; AW494740; C80642; mKIAA1386
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228427 representing NM_001289529
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCCAGAAGAACTCGTTGGAGTCAGCGAAGCCTATGAAGATGCTGCCAATTGTCTGTGGCTGTTAA
  CTAACTCCAAGCCTTGTGCGAACTGTAAGTCTCCAATACAGAAGAACGAAGGCTGTAATCACATGCAGTG
  TGCCAAGTGCAAGTATGACTTTTGCTGGATTTGCCTAGAAGAATGGAAAAAACATAGTTCTTCCACTGGA
  GGTTACTACAGATGCACTCGCTATGAAGTCATCCAACATGTGGAGGAGCAATCCAAGGAGATGACAGTGG
  AGGCTGAGAAGAAACATAAACGGTTTCAGGAACTTGACAGATTTATGCACTATTATACAAGATATAAAAA
  CCATGAGCATAGTTATCAGTTAGAACAACGCCTTCTTAAAACAGCCAAAGAAAAGATGGAGCAGTTGAGT
  AGAGCTCTCAAAGAAACTGAAGGAGGCTGTCCAGATACCACTTTCATCGAAGATGCAGTGCATGTTCTTC
  TAAAAACTCGGCGCATCCTCAAGTGTTCTTATCCATATGGATTTTTCTTAGAACCTAAAAGCACAAAGAA
  AGAAATTTTTGAACTAATGCAAACAGACCTGGAAATGGTCACTGAAGACCTTGCCCAGAAAGTCAATAGG
  CCTTATCTTCGTACACCTCGGCACAAGATCATCAGGGCAGCATGCCTTGTGCAGCAGAAAAGGCAAGAGT
  TCCTGGCATCTGTCGCTCGTGGTGTTGCTCCTGCAGACTCACCAGATGCTCCCAGGCGCAGCTTTGCCGG
  TGGAACATGGGATTGGGAATATTTAGGATTTGCTTCACCTGAGGAATATGCTGAATTTCAGTATCGGAGG
  AGACACAGACAGCAGCGCCGTCGAGGAGATGTGCATAGCCTGCTCAGTAACCCTACAGACCTGGATGAAC
  CAAGCGAAAGCACTTTCGATCTCCCAGAGGGGAGCAGCGGCCGCAGGCCTGGTGCATCTGTGGTCAGCTC
  TGCATCGATGAGTGTGCTGCACAGCTCTTCCCTGCGAGACTACAGCCCTGCCAGTCGCTCTGCAAACCAG
  GACTCTCTTCAGGCTCTGAGTTCCTTGGATGAAGATGATCCCAACATACTCCTGGCCATCCAGTTGTCAC
  TGCAAGAGTCTGGGCTGGACATGGATGAAGAGACCAGAGACTTCCTTAGTAACGAGACATCCCTAGGAGC
  CATAGGCTCATCTTTACCTTCCCGACTGGACTCTGTGCCCCGCAGCACTGAGTCTCCTCGGGCTGCGCTG
  AGCAGCTCGGAGCTGCTGGAGCTTGGTGACAGTCTCATGAGACTAGGAGCAGACAGTGACCCATTTTCAA
  CTGACACTTTGAGTTCACGCCCTCTCAGTGAGACAAGAAGTGATTTCTGTCCCTCTTCCAGTGACCTTGA
  CTCAGCTGGCCAGGACCCCAGTGCCAATGACAATCTTCTTGGCAATATTATGGCTTGGTTTCATGACATG
  AACCCGCAGAGTATCGCCCTGATTCCTCCAGCAGCAACCACAGAAATTAGTGCTGAGCCTCAGCTTCCTT
  GTATAAGAGATGGGTCTGAAGGTGTGAGGGACATGGAACTCGTGCCACCAGAAGACTCAGTGTCCAAAGA
  TACTGGTGTTCATGAAGGTGAAAGAGCCCAGATGGAAGAAAATCCTCTGGAAGAGAATATTCTGGCCAGG
  GAAGAGTTGTCTCAAGCTGGTGACAGTAGTAACGAGGCAGTGGGCAGGGGGGACAGGCCAGATGCTGCCA
  GTCAAACCCCTCAGACCTCAAGCGACTGGCTCGAGCAAGTACATTCAGTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289529
• Insert Size: 1803 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289529.1, NP_001276458.1
• RefSeq Size: 6253 bp
• RefSeq ORF: 1803 bp
• Locus ID: 70797
• UniProt ID: Q6ZPS6
• Cytogenetics: 5 A1
• Gene Summary: Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) lacks an exon in the 5' UTR and contains an alternate exon in the 5' region, compared to variant 1. It initiates translation at a downstream in-frame start codon. The encoded isoform (4) has a shorter N-terminus than isoform 1.