Numb (NM_001272056) Mouse Tagged ORF Clone

CAT#: MR230616

Numb (myc-DDK-tagged) - Mouse numb gene homolog (Drosophila) (Numb), transcript variant 4

Product Images

Circular map for MR230616

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Numb
• Synonyms: m-num; Nb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230616 representing NM_001272056
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAAACTACGGCAAAGCTTCAGGAGAAAGAAAGACGTTTATGTCCCAGAGGCCAGCCGTCCACATC
  AGTGGCAGACAGATGAAGAAGGAGTCCGCACTGGAAAGTGTAGCTTCCCAGTTAAGTACCTCGGCCACGT
  AGAAGTTGATGAGTCAAGAGGAATGCACATCTGTGAAGATGCCGTAAAGAGATTGAAAGCTACGGGAAAG
  AAAGCAGTGAAGGCCGTTCTGTGGGTGTCAGCGGATGGGCTCAGAGTTGTGGACGAGAAAACTAAGGACC
  TCATAGTTGACCAGACAATAGAAAAAGTTTCCTTCTGTGCCCCAGATAGGAACTTTGACAGAGCCTTTTC
  TTACATATGTCGTGATGGCACCACTCGGCGATGGATCTGTCATTGTTTCATGGCTGTCAAAGACACGGGG
  GAAAGACTGAGCCACGCCGTGGGCTGTGCTTTTGCAGCCTGTTTAGAGCGTAAACAGAAGCGGGAGAAGG
  AGTGTGGCGTCACTGCTACTTTTGATGCCAGTAGAACCACTTTCACAAGAGAAGGATCATTCCGTGTCAC
  AACTGCCACTGAGCAAGCCGAAAGAGAGGAGATCATGAAACAGTTGCAAGATGCCAAGAAAGCTGAGACA
  GACAAGACAGTTGTTGGTCCATCAGTGGCTCCTGGCAACACTGCTCCATCCCCATCCTCTCCCACCTCTC
  CCACTCCGGATGGCACTGCATCTTCAGAGATGAACAATCCCCATGCTATCCCACGCCGGCATGCACCAAT
  TGAACAGCTTGCTCGTCAAGGCTCTTTCCGGGGATTTCCTGCTCTTAGCCAGAAGATGTCACCCTTTAAA
  CGCCAGCTGTCCCTACGCATCAATGAGTTGCCTTCCACTATGCAGAGGAAGACCGATTTCCCAATAAAAA
  ACACAGTGCCCGAGGTGGAAGGAGAGGCCGAGAGCATCAGCTCCTTGTGTTCCCAGATCACCAGTGCCTT
  CAGCACGCCCTCTGAGGACCCCTTCTCCTCCGCCCCAATGACCAAACCAGTGACATTGGTGGCACCACAG
  TCTCCTGTGTTACAAGGGACTGAGTGGGGTCAGTCTTCTGGTGCTGCCTCTCCAGGTCTCTTCCAGGCTG
  GTCACAGACGCACTCCCTCTGAAGCTGACCGCTGGTTAGAAGAAGTGTCAAAGAGTGTGCGGGCCCAGCA
  GCCTCAGGTCTCAGCTGCCCCTCTGCAGCCAGTTCTCCAGCCGCCTCCGCCCGCCGCCATTGCCCCTCCA
  GCACCTCCTTTCCAAGGACATGCCTTCCTCACGTCCCAGCCTGTGCCCGTGGGTGTGGTCCCACCCCTAC
  AACCAGCCTTTGTCCCTACCCAGTCCTACCCTGTGGCCAACGGGATGCCCTACCCAGCCTCTAATGTGCC
  TGTAGTGGGCATCACCCCATCCCAGATGGTAGCCAATGTGTTTGGCACTGCAGGCCACCCTCAGACAACT
  CATCCACATCAGTCGCCAAGCCTGGCCAAGCAGCAGACATTCCCTCAATATGAGACAAGTAGTGCTACCA
  CCAGTCCCTTCTTTAAGCCTCCTGCTCAGCACCTCAATGGTTCTGCAGCTTTCAATGGTGTAGACAATGG
  TGGGCTAGCCTCAGGAAACAGGCATGCAGAAGTCCCTCCAGGCACCTGCCCAGTGGATCCTTTCGAAGCT
  CAGTGGGCCGCACTAGAAAGCAAGTCCAAGCAGCGTACAAATCCTTCTCCTACCAACCCTTTCTCCAGTG
  ACTTACAGAAAACATTTGAAATAGAACTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230616 representing NM_001272056
  Red=Cloning site Green=Tags(s)
  
  MNKLRQSFRRKKDVYVPEASRPHQWQTDEEGVRTGKCSFPVKYLGHVEVDESRGMHICEDAVKRLKATGK
  KAVKAVLWVSADGLRVVDEKTKDLIVDQTIEKVSFCAPDRNFDRAFSYICRDGTTRRWICHCFMAVKDTG
  ERLSHAVGCAFAACLERKQKREKECGVTATFDASRTTFTREGSFRVTTATEQAEREEIMKQLQDAKKAET
  DKTVVGPSVAPGNTAPSPSSPTSPTPDGTASSEMNNPHAIPRRHAPIEQLARQGSFRGFPALSQKMSPFK
  RQLSLRINELPSTMQRKTDFPIKNTVPEVEGEAESISSLCSQITSAFSTPSEDPFSSAPMTKPVTLVAPQ
  SPVLQGTEWGQSSGAASPGLFQAGHRRTPSEADRWLEEVSKSVRAQQPQVSAAPLQPVLQPPPPAAIAPP
  APPFQGHAFLTSQPVPVGVVPPLQPAFVPTQSYPVANGMPYPASNVPVVGITPSQMVANVFGTAGHPQTT
  HPHQSPSLAKQQTFPQYETSSATTSPFFKPPAQHLNGSAAFNGVDNGGLASGNRHAEVPPGTCPVDPFEA
  QWAALESKSKQRTNPSPTNPFSSDLQKTFEIEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001272056
• ORF Size: 1779 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001272056.1, NP_001258985.1
• RefSeq Size: 3432 bp
• RefSeq ORF: 1782 bp
• Locus ID: 18222
• UniProt ID: Q9QZS3
• Cytogenetics: 12 D1
• MW: 64.8 kDa
• Gene Summary: This gene encodes a conserved protein that is distributed asymmetrically during cell division in the developing embryo. The encoded protein participates in cell fate decisions by interacting with the Notch receptor. Loss of function of this gene results in severe defects in neural development and loss of viability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]