Miga2 (NM_001242407) Mouse Tagged ORF Clone

CAT#: MR230614

Fam73b (myc-DDK-tagged) - Mouse family with sequence similarity 73, member B (Fam73b), transcript variant 2

Product Images

Circular map for MR230614

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Miga2
• Synonyms: 5730472N09Rik; AA408683; AI790341; C9orf54; Fam73b; R74766
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230614 representing NM_001242407
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATTCCGGAGGACTGAGGGTATGTCCATGATCCAGGCTCTGGCCATGACTGTGGCTGAGATTCCAG
  TGTTCCTGTACACAACTTTTGGTCAGTCCGCGTTCTCCCAGCTGCGGTTGACACCGGGCCTGAGGAAGGT
  TCTTTTTGCCACAGCCCTTGGGACTGTGGCCTTGGCCCTGGCTGCCCACCAGCTGAAAAGGCGCCGGCGG
  AAGAAGAAGCAGGTGGGCCCTGAGATGGGAGGTGAGCAGCTGGGCACGGTGCCCATGCCCATCCTCATGG
  CCCGCAAGGTCCCTTCGGTGAAGAAAGGCTGCTCCAGCAGGAGGGTTCAGAGCCCCAGCAGCAAGAGCAA
  CGACACTCTTAGTGGCATCTCCTCCATCGAGCCCAGCAAGCACTCAGGCTCCTCCCACAGCCTGGCCTCG
  ATGGTAGTGGTCAATTCATCCAGCCCTACAGCTGCGTGCTCAGGTTCATGGGAAGCCCGAGGGATGGAGG
  AGTCTGTGCCCACCACTGATGGCAGTGCCGAGAGCCTCTATGTGCAAGGCATGGAGCTGTTTGAGGAAGC
  CCTGCAGAAATGGGAGCAGGCACTGAGTGTGGGCCAGAGAGGGGATGGAGGCAGCACCCCCACACCAGGC
  GACAGCCTCCAGAACCCAGACACCGCATCAGAAGCACTGTCAGAGCCAGAGTCCCAACGAAGGGAGTTTG
  CTGAGAAGCTGGAGTCCCTGCTGCACCGGGCCTACCACCTGCAGGAAGAGTTTGGCTCTACCTTCCCCTC
  TGACAGCATGCTGTTGGATCTTGAGAGGACCCTAATGCTGCCACTGACTGAGGGCTCACTGCGACTTCGA
  GCTGACGATGAGGACAGCCTGACCTCTGAAGACTCCTTTTTCTCAGCCACTGAGATCTTTGAGTCCCTGC
  AGATCGGCGAGTACCCGCTTCCTCTCTCCAGGCCTGCTGCTGCCTACGAGGAGGCTCTGCAGCTGGTGAA
  GGAAGGAAGAGTCCCTTGCCGAACACTCAGGACAGAGCTGCTGGGCTGCTACAGTGACCAGGACTTCCTA
  GCCAAGCTGCATTGTGTACGGCAGGCCTTTGAGGGGCTTCTAGAAGAAAGAAGCAACCAGATCTTCTTTG
  GGGAGGTCGGCCGGCAGATGGTGACGGGCCTGATGACCAAGGCTGAGAAGAGTCCCAAAGGCTTCCTGGA
  GAGCTATGAGGAGATGCTGAGCTACGCCCTGCGGCCTGAGACCTGGGCTACCACCCGGCTGGAGCTGGAG
  GGCAGAGGGGTGGCCTGCATGAGCTTCTTCGACATCGTGCTGGACTTCATCCTCATGGATGCCTTTGAGG
  ACCTAGAGAACCCTCCGTCTTCGGTGCTTGCTGTCCTGAGGAACCGCTGGCTGTCTGACAGCTTCAAAGA
  AACAGCCCTAGCCACCGCGTGCTGGTCTGTCCTGAAAGCCAAGAGGAGGCTCCTGATGGTGCCTGATGGC
  TTCATCTCCCATTTCTACTCCGTATCGGAGCATGTTAGCCCTGTGTTGGCCTTTGGCTTCCTCGGACCCA
  AGCCCCAGCTCTCGGAAGTCTGTGCTTTCTTCAAGCACCAGATTGTACAATACCTGAGGGACATGTTCGA
  CCTGGACAACGTGCGCTACACATCTGTGCCAGCCCTAGCAGAAGACATCCTACAGCTGTCCCGGCGGCGC
  AGCGAGATTCTATTGGGCTACCTGGGGGCACCTGTGGCTAGTAGCATCGGGCTGAATGGGCCACTGCCTC
  GAGAAAATGGGCCCCTGGAAGAGCTGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230614 representing NM_001242407
  Red=Cloning site Green=Tags(s)
  
  MAFRRTEGMSMIQALAMTVAEIPVFLYTTFGQSAFSQLRLTPGLRKVLFATALGTVALALAAHQLKRRRR
  KKKQVGPEMGGEQLGTVPMPILMARKVPSVKKGCSSRRVQSPSSKSNDTLSGISSIEPSKHSGSSHSLAS
  MVVVNSSSPTAACSGSWEARGMEESVPTTDGSAESLYVQGMELFEEALQKWEQALSVGQRGDGGSTPTPG
  DSLQNPDTASEALSEPESQRREFAEKLESLLHRAYHLQEEFGSTFPSDSMLLDLERTLMLPLTEGSLRLR
  ADDEDSLTSEDSFFSATEIFESLQIGEYPLPLSRPAAAYEEALQLVKEGRVPCRTLRTELLGCYSDQDFL
  AKLHCVRQAFEGLLEERSNQIFFGEVGRQMVTGLMTKAEKSPKGFLESYEEMLSYALRPETWATTRLELE
  GRGVACMSFFDIVLDFILMDAFEDLENPPSSVLAVLRNRWLSDSFKETALATACWSVLKAKRRLLMVPDG
  FISHFYSVSEHVSPVLAFGFLGPKPQLSEVCAFFKHQIVQYLRDMFDLDNVRYTSVPALAEDILQLSRRR
  SEILLGYLGAPVASSIGLNGPLPRENGPLEELQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001242407
• ORF Size: 1779 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001242407.1, NP_001229336.1
• RefSeq Size: 3394 bp
• RefSeq ORF: 1782 bp
• Locus ID: 108958
• UniProt ID: Q8BK03
• Cytogenetics: 2 B
• MW: 65.5 kDa
• Gene Summary: Regulator of mitochondrial fusion (PubMed:26711011). Acts by forming homo- and heterodimers at the mitochondrial outer membrane and facilitating the formation of PLD6/MitoPLD dimers. May act by regulating phospholipid metabolism via PLD6/MitoPLD (By similarity).[UniProtKB/Swiss-Prot Function]