Lrrtm4 (NM_001134743) Mouse Tagged ORF Clone

CAT#: MR230608

Lrrtm4 (myc-DDK-tagged) - Mouse leucine rich repeat transmembrane neuronal 4 (Lrrtm4), transcript variant 3

Product Images

Circular map for MR230608

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lrrtm4
• Synonyms: 7530419J18Rik; A230052N11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230608 representing NM_001134743
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGGTTTCCGTCTAATCACGCAGCTGAAAGGCATGAGTGTGTTTCTGGTGCTATTTCCCACCCTGC
  TGCTGGTTATGCTGACGGGGGCTCAGAGAGCTTGCCCAAAGAACTGCAGATGCGATGGCAAGATTGTATA
  CTGTGAGTCTCACGCCTTCGCAGACATCCCTGAGAACATTTCTGGAGGGTCACAAGGTTTATCCTTAAGG
  TTCAACAGCATTCAGAAACTCAAATCCAATCAGTTTGCCGGCCTTAACCAGCTTATATGGCTTTATCTTG
  ACCATAATTACATTAGCTCAGTGGACGAAGATGCCTTTCAAGGGATCCGCAGACTGAAAGAATTAATTCT
  AAGCTCCAACAAAATTACCTATCTGCACAATAAAACATTTCACCCAGTCCCCAACCTCCGCAACCTGGAT
  CTCTCCTACAACAAACTTCAGACCTTACAGTCTGAGCAGTTTAAAGGCCTCCGGAAACTCATCATTTTGC
  ACTTGCGATCTAATTCTCTCAAGACCGTGCCCATACGGGTTTTTCAAGACTGCCGGAACCTTGACTTTCT
  GGATTTGGGTTATAATCGGCTCCGAAGCTTATCCCGAAACGCGTTTGCCGGTCTTCTGAAGTTAAAAGAG
  CTCCACCTGGAGCATAATCAGTTTTCAAAGATCAACTTTGCTCATTTTCCTCGTCTCTTCAACCTGCGAT
  CCATCTACCTGCAATGGAACAGGATCCGCTCTGTCAGTCAAGGCCTAACATGGACCTGGAGTTCCTTGCA
  CACCTTGGATTTATCAGGGAATGACATCCAAGCAATCGAGCCTGGCACATTTAAATGCCTCCCCAATTTG
  CAAAAATTGAATTTGGATTCCAACAAGCTCACCAATGTCTCCCAGGAGACTGTCAATGCATGGATATCCT
  TAATATCCATCACCTTGTCTGGAAACATGTGGGAATGTAGTCGGAGCATCTGTCCTCTATTCTACTGGCT
  GAAGAATTTCAAAGGGAATAAAGAAAGTACCATGATATGTGCAGGGCCTAAGCACATACAGGGTGAGAAG
  GTTAGCGATGCCGTGGAGACTTACAACATCTGCTCTGATGTCCAGGTGGTCAACACAGAACGATCACATC
  TGGCGCCTCAAACTCCCCAGAAGCCCCCGTTCATCCCCAAACCTACCATCTTCAAACCGGATGCAGTCCC
  AGCCACCCTAGAGGCAGTGAGCCCATCCCCGGGCTTTCAAATTCCAGGTACGGATCATGAGTACGAGCAC
  GTGTCGTTTCACAAAATCATCGCAGGGAGCGTGGCTCTGTTCCTCTCCGTGGCCATGATTCTCCTGGTGA
  TCTATGTGTCTTGGAAACGGTACCCAGCCAGCATGAAACAACTCCAACAGCACTCACTTATGAAGAGGAG
  ACGGAAAAAGGCGAGGGAGTCGGAGAGACAGATGAATTCCCCTTTACAGGAATACTACGTGGACTACAAA
  CCGACGAACTCTGAGACCATGGATATATCGGTTAATGGATCTGGTCCCTGCACATATACCATCTCTGGCT
  CCAGGGAGTGTGAGATACCCCACCATGTGAAGCCCCTGCCGTATTACAGCTATGACCAACCAGTGATTGG
  GTACTGCCAGGCCCACCAGCCTCTCCACATCAACAAGGCTTATGAAGCTGTGTCTATAGAACAGGATGAC
  AGCCCCAGTTTGGAGCTTGGGAGAGACCACAGCTTCATTGCCACCATAGCCAGGTCGGCTGCACCTGCCA
  TTTATCTGGAGAGAATAACAAAC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230608 representing NM_001134743
  Red=Cloning site Green=Tags(s)
  
  MPGFRLITQLKGMSVFLVLFPTLLLVMLTGAQRACPKNCRCDGKIVYCESHAFADIPENISGGSQGLSLR
  FNSIQKLKSNQFAGLNQLIWLYLDHNYISSVDEDAFQGIRRLKELILSSNKITYLHNKTFHPVPNLRNLD
  LSYNKLQTLQSEQFKGLRKLIILHLRSNSLKTVPIRVFQDCRNLDFLDLGYNRLRSLSRNAFAGLLKLKE
  LHLEHNQFSKINFAHFPRLFNLRSIYLQWNRIRSVSQGLTWTWSSLHTLDLSGNDIQAIEPGTFKCLPNL
  QKLNLDSNKLTNVSQETVNAWISLISITLSGNMWECSRSICPLFYWLKNFKGNKESTMICAGPKHIQGEK
  VSDAVETYNICSDVQVVNTERSHLAPQTPQKPPFIPKPTIFKPDAVPATLEAVSPSPGFQIPGTDHEYEH
  VSFHKIIAGSVALFLSVAMILLVIYVSWKRYPASMKQLQQHSLMKRRRKKARESERQMNSPLQEYYVDYK
  PTNSETMDISVNGSGPCTYTISGSRECEIPHHVKPLPYYSYDQPVIGYCQAHQPLHINKAYEAVSIEQDD
  SPSLELGRDHSFIATIARSAAPAIYLERITN
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001134743
• ORF Size: 1773 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001134743.1, NP_001128215.1
• RefSeq Size: 3054 bp
• RefSeq ORF: 1776 bp
• Locus ID: 243499
• UniProt ID: Q80XG9
• Cytogenetics: 6 C3
• MW: 67.2 kDa
• Gene Summary: May play a role in the development and maintenance of the vertebrate nervous system. Exhibits strong synaptogenic activity, restricted to excitatory presynaptic differentiation (By similarity).[UniProtKB/Swiss-Prot Function]