Enox2 (NM_001271451) Mouse Tagged ORF Clone

CAT#: MR230585

Enox2 (myc-DDK-tagged) - Mouse ecto-NOX disulfide-thiol exchanger 2 (Enox2), transcript variant 6

Product Images

Circular map for MR230585

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Enox2
• Synonyms: APK1; Cova1; tNOX
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230585 representing NM_001271451
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGCTGCCTGTGTCTGATCCAGCTGCATGGGCCACAGCAATGAATAATCTTGGAATGGCTCCACTGG
  GAATTGCTGGACAACCAATTTTACCTGACTTCGATCCTGCCCTTGGGATGATGACTGGAATACCACCAAT
  AACTCCCATGATGCCGGGTTTGGGCATAGTCCCGCCACCGATTCCTCCAGATATGCCGGTAGCAAAGGAG
  ATCATACACTGCAAAAGCTGCACGCTCTTCCCTCCCAACCCAAATCTTCCACCACCTGCAACACGAGAAA
  GGCCACCAGGCTGTAAGACAGTGTTTGTGGGTGGCCTGCCTGAAAATGGGACAGAGCAGATCATTGTGGA
  AGTGTTTGAACAGTGTGGAGAGATTATTGCTATCCGGAAGAGCAAAAAGAACTTCTGTCACATTCGCTTT
  GCTGAGGAATACATGGTGGACAAAGCCCTCTATCTGTCTGGTTACCGAATTCGTCTGGGCTCTAGTACTG
  ACAAGAAGGACACAGGCCGGCTCCATGTTGACTTTGCCCAGGCTCGGGATGACTTATATGAGTGGGAGTG
  TAAACAGCGTATGCTAGCCAGAGAGGAGCGGCACCGTAGAAGGATGGAAGAAGAAAGAATGCGTCCACCA
  TCCCCACCTCCAGTGGTCCACTATTCAGATCATGAATGCAGCATTGTTGCTGAAAAACTCAAAGATGATT
  CCAAATTCTCGGAAGCTGTGCAGACCTTGCTCACCTGGATTGAGAGGGGGGAAGTGAACCGCCGCAGTGC
  CAACCACTTCTACTCCATGATCCAGTCAGCCAACAGCCATGTCCGCCGCCTGGTAAATGAGAAAGCTACC
  CATGAGAAAGAGATGGAAGAAGCAAAAGAGAAGTTCAAGCAGGCCCTTTCTGGAATTCTCATTCAATTTG
  AGCAGATAGTAGCTGTGTACCATTCCGCTTCCAAACAGAAGGCATGGGACCACTTCACAAAAGCACAACG
  TAAAAACATCAGTGTTTGGTGCAAACAAGCTGAGGAAATTCGCAACATTCATAATGATGAATTAATGGGA
  ATCAGAAGAGAAGAAGAAATGGAAATGTCTGATGATGAAATAGAAGAGACAACAGAAACAAAAGAAACGG
  AGGAATCAGCCTTAGTGTCACAGGCAGAAGCTCTGAAGGAGGAAAATGATAGCCTCCGCTGGCAGCTGGA
  CGCATACCGGAATGAGGTAGAACTGCTCAAACAGGAACAAGGCAAAGCCCACAGGGAGGATGACCCAAAC
  AAGGAACAGCAGCTGAAACTTCTGCAGCAAGCCCTACAAGGGATGCAACAGCATCTGCTCAAAGTCCAAG
  AGGAATACAAAAAGAAAGAAGCTGAGCTTGACCGAATCAAAGACGACAATTTACAGGTAGAACAACTGTT
  GGAAAATTTTCATGAAAAGCAGGAAAACTGTGGTTCTAGACTGTGTGCATCAAGCCAAGAAGGTGAGCAA
  CCTCTTGAGAAGACCGCAGTCAGCAATCCTGTCAAATCTGAACGTGAGGCACTGCTAGTGGGGATCATCT
  CCACATTCCTTCACGTCCACCCATTTGGAGCCAGCATTGAATACATCTGTTCCTACTTGAATCGTCTCGA
  TAATAAGATCAGCACCAGTGATGTGGAATCTCTCATGAGTAGACTCCAGCATACCTTCAGACAGGAAATG
  ACTGGAGTTGGAGCCAGCCTGGAGAAGAGATGGAAATTCTGTGGTTTTGAGGGCTTGAAGCTGACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230585 representing NM_001271451
  Red=Cloning site Green=Tags(s)
  
  MTLPVSDPAAWATAMNNLGMAPLGIAGQPILPDFDPALGMMTGIPPITPMMPGLGIVPPPIPPDMPVAKE
  IIHCKSCTLFPPNPNLPPPATRERPPGCKTVFVGGLPENGTEQIIVEVFEQCGEIIAIRKSKKNFCHIRF
  AEEYMVDKALYLSGYRIRLGSSTDKKDTGRLHVDFAQARDDLYEWECKQRMLAREERHRRRMEEERMRPP
  SPPPVVHYSDHECSIVAEKLKDDSKFSEAVQTLLTWIERGEVNRRSANHFYSMIQSANSHVRRLVNEKAT
  HEKEMEEAKEKFKQALSGILIQFEQIVAVYHSASKQKAWDHFTKAQRKNISVWCKQAEEIRNIHNDELMG
  IRREEEMEMSDDEIEETTETKETEESALVSQAEALKEENDSLRWQLDAYRNEVELLKQEQGKAHREDDPN
  KEQQLKLLQQALQGMQQHLLKVQEEYKKKEAELDRIKDDNLQVEQLLENFHEKQENCGSRLCASSQEGEQ
  PLEKTAVSNPVKSEREALLVGIISTFLHVHPFGASIEYICSYLNRLDNKISTSDVESLMSRLQHTFRQEM
  TGVGASLEKRWKFCGFEGLKLT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271451
• ORF Size: 1746 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271451.1, NP_001258380.1
• RefSeq Size: 3918 bp
• RefSeq ORF: 1749 bp
• Locus ID: 209224
• Cytogenetics: X A5
• MW: 67 kDa
• Gene Summary: May be involved in cell growth. Probably acts as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock (By similarity).[UniProtKB/Swiss-Prot Function]