Dtna (NM_001285811) Mouse Tagged ORF Clone

CAT#: MR230547

Dtna (myc-DDK-tagged) - Mouse dystrobrevin alpha (Dtna), transcript variant 7

Product Images

Circular map for MR230547

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dtna
• Synonyms: 2210407P21Rik; a-DB-1; adbn; Dtn; DTN-A; Gm19389
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230547 representing NM_001285811
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTGAAGATAGTGGAAAAAGAGGAAACACCATGGCAGAAAGAAGACAGCTGTTTGCAGAGATGAGGG
  CTCAAGATCTGGATCGCATCCGACTCTCCACCTACAGAACAGCATGCAAGCTGAGATTTGTGCAGAAGAA
  ATGCAATTTGCACCTGGTGGACATTTGGAACGTCATTGAAGCATTGCGCGAAAACGCTTTGAATAACCTG
  GACCCAAACATAGAACTCAACGTGGCCCGCCTGGAGGCGGTGCTCTCCACTATTTTTTACCAGCTCAACA
  AGAGGATGCCAACCACTCACCAAATCCACGTGGAGCAGTCCATCAGTCTCCTGCTGAACTTCCTGCTCGC
  AGCCTTTGACCCGGAAGGCCATGGAAAAATCTCAGTATTTGCTGTCAAAATGGCTTTGGCTACATTGTGT
  GGAGGAAAGATCATGGACAAGTTAAGATATATTTTCTCAATGATCTCTGACTCCAGTGGAGTGATGGTAT
  ATGGAAGATATGACCAATTCCTTCGGGAAGTTCTCAAACTACCCACAGCAGTTTTTGAAGGTCCTTCATT
  TGGTTACACAGAACAGTCAGCCAGATCCTGTTTCTCCCAACAGAAAAAGGTCACCTTAAATGGCTTCTTG
  GACACGCTCATGTCAGACCCTCCTCCACAGTGCCTGGTATGGCTGCCTCTTCTGCACCGACTGGCAAATG
  TAGAAAATGTCTTCCATCCAGTTGAGTGTTCCTACTGTCACAGTGAGAGCATGATGGGATTTCGATACCG
  ATGTCAACAGTGTCACAACTACCAGCTTTGCCAGGACTGCTTCTGGAGGGGCCATGCAGGAGGTTCTCAT
  AGCAACCAGCACCAAATGAAGGAGTATACGTCGTGGAAATCACCTGCTAAGAAGCTAACGAATGCATTAA
  GCAAGTCCCTGAGCTGTGCTTCAAGCCGTGAACCTTTGCACCCCATGTTCCCAGACCAGCCTGAAAAGCC
  ACTCAACTTGGCTCACATCGTGCCTCCGAGACCTGTAACCAGCATGAACGACACACTCTTTTCCCACTCT
  GTTCCCTCCTCAGGAAGTCCTTTCATCACCAGGAGCTCTCCTCCCAAGGACAGTGAAGTAGAACAGAACA
  AAATGCTGGCTAGGGCTGCTCCCGCCTTTCTGAAGGGCAGAGGGATACAGTACAGCCTGAATGTGGCAGA
  CAGGCTAGCTGATGAACATGTTCTCATTGGGTTGTATGTCAACATGCTCCGGAACAACCCACCATGTATG
  CTTGAGAGTTCAAACCGGCTTGATGAAGAACACAGGCTGATCGCCAGGTACGCCGCACGACTGGCAGCAG
  AATCCTCCTCATCACAGCCCACACAGCAGAGGAGTGCCCCTGACATCTCCTTCACCATCGATGCAAATAA
  ACAGCAAAGGCAGCTGATTGCAGAGCTAGAGAACAAGAACAGAGAAATCTTACAAGAGATTCAGAGACTT
  CGGGTAGAACATGAGCAAGCTTCCCAGCCCACGCCAGAGAAAGCTCAGCAGAACCCAACCTTGCTGGCAG
  AACTCCGGCTCCTCAGACAGCGAAAGGATGAGCTAGAACAGAGAATGTCTGCTCTCCAGGAGAGCCGGAG
  AGAGCTGATGGTCCAGTTGGAAGGTCTCATGAAGCTCCTGAAGGAAGAAGAACTGAAGCAGGGAGTAATG
  CATGAAATTATCCCCCTTGAGGAGAGAACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230547 representing NM_001285811
  Red=Cloning site Green=Tags(s)
  
  MIEDSGKRGNTMAERRQLFAEMRAQDLDRIRLSTYRTACKLRFVQKKCNLHLVDIWNVIEALRENALNNL
  DPNIELNVARLEAVLSTIFYQLNKRMPTTHQIHVEQSISLLLNFLLAAFDPEGHGKISVFAVKMALATLC
  GGKIMDKLRYIFSMISDSSGVMVYGRYDQFLREVLKLPTAVFEGPSFGYTEQSARSCFSQQKKVTLNGFL
  DTLMSDPPPQCLVWLPLLHRLANVENVFHPVECSYCHSESMMGFRYRCQQCHNYQLCQDCFWRGHAGGSH
  SNQHQMKEYTSWKSPAKKLTNALSKSLSCASSREPLHPMFPDQPEKPLNLAHIVPPRPVTSMNDTLFSHS
  VPSSGSPFITRSSPPKDSEVEQNKMLARAAPAFLKGRGIQYSLNVADRLADEHVLIGLYVNMLRNNPPCM
  LESSNRLDEEHRLIARYAARLAAESSSSQPTQQRSAPDISFTIDANKQQRQLIAELENKNREILQEIQRL
  RVEHEQASQPTPEKAQQNPTLLAELRLLRQRKDELEQRMSALQESRRELMVQLEGLMKLLKEEELKQGVM
  HEIIPLEERT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001285811
• ORF Size: 1710 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285811.1, NP_001272740.1
• RefSeq Size: 2152 bp
• RefSeq ORF: 1713 bp
• Locus ID: 13527
• Cytogenetics: 18 12.08 cM
• MW: 65.6 kDa
• Gene Summary: Involved in synapse maturation and required for normal muscle function.[UniProtKB/Swiss-Prot Function]