Rad21l (NM_001276400) Mouse Tagged ORF Clone

CAT#: MR230482

Rad21l (myc-DDK-tagged) - Mouse RAD21-like (S. pombe) (Rad21l)

Product Images

Circular map for MR230482

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rad21l
• Synonyms: Gm14160; MeiRAD21L; Rad21l1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230482 representing NM_001276400
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTACACTCATGTGCTTATGAGTAAACGGGGGCCATTGGCCAAGATATGGCTTGCAGCTCACTGGG
  AGAAGAAACTTACAAAGGCCCATGTGTTCGAGTGTAATCTAGAAATAACCATTCAAAAAATTATTTCACC
  TAAGGTGAAAATTGCACTCCGAACTTCAGGACACCTTCTTTTGGGGGTTGTTCGAATCTACAACAGGAAG
  GCAAAATACCTTCTGGCAGACTGCAGTGAAGCATTCCTTAAGATGAAGATGACATTTCGCCCAGGACTGG
  TTGACCTCCCCAAAGAGAATTTTGAAGCAGCTTACAACACTATCACATTACCAGAAGAATTTCATGATTT
  TGAAATCTACAATATAAATGAGATTGATATTTCGGAGCCCCTTGCTCAGAACCAAAGTAGACCAGAGGAA
  ATCACACTTAGAGAAGAATATAGCAATGATCTACTTTTCCAAGCTGGGAGCTTTGGGGATGAACCTGAAA
  TCCTCAGAAGACATAGCTTCTTTGATGACAACATATTAATGAACTCCAGTGGTCTTGTAGTCGAACATAG
  TTCTGGAAGCTTCGCTGAGGAAAAATCTTTGTTCTTTGACAATGGAGATGGATTTGGAGATGAAGGAGCT
  GCAGGAGAAATGATTGACAATCTATTGCAAGATGAGAGTACCTTTTTAGAAGAAGCATATTTGAACAAAG
  AAGTGTCTCTGCCTCCTGAGCTTCCCAGTAGTATAATGGTAGAACCAGGTAATTCAGATGACCAATGTAT
  ACCTGAAGATGAAGAAATAAATGAAATAACATTATTATCAAATGAAGATGAAGGATTTACTCTGGACCCA
  ATTGATGATTTAGATATTGCTGACAGGAGGAGAAGGAAAAAGAGGAGATTGCTGGTAGATCCAGTCAAGG
  AGATAAGCAGCAAGGCTATGCATAGGCAGCTTGCTTCCTTTATGGACACTCTCATGGTTCTGGACCTTGC
  ACCCCCCACACAAAGATTGATGATGTGGAAGAAGCGGGGAGGAGTGGATATGCTTCTGTCAACTGCTACC
  CAGGATTTGATTAATGACGAATTGAAAATGTTGTTTACAAAATGCTTCCTGTCCTCTGACTATAAACTTG
  CAAAATTGACACTGAAGGAGTCAGTAAGGAAAGAAGTGGGAAACCAGCAGATTGCAGAACCCTCAGTGAT
  GGGAGAGCCAAATTCCCACAGTGAGTTAGATCAACCCCAAGATTGGAAGGATGTGACTGATGAATCTGTG
  GGTAGCTTTCAGGAGAACGTCAACATGAATGTTAACTCTGAGCAGGATATTCTTGGAATGATTTCTCCAG
  CTGTTGAGGGATTGTCTTCAATGAATGGGTCCTTGGCCCAAGAAAATTGTCCAGCTGAATTGGAGTCATC
  AGGCAGCAAACAAAATACTGAGGCAGAAAAATGGAATCAAAGATTATTTCAAACATTAAATGTGTTACGG
  GAATTCAACAAAATGGGAATGCAGTCCTTTAGTCTGAAAAAGCTATGCAGAAATAGTGACCGAAAACAGG
  CAGCTGCCAAATTTTATACTCTTCTCATCCTGAAAAAACATCGTGCTATTGAGCTGAGCCAGAGTGTTCC
  CTATGCAGATATTATAGCTACAGTGGGACCAATGTTCTATAAGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230482 representing NM_001276400
  Red=Cloning site Green=Tags(s)
  
  MFYTHVLMSKRGPLAKIWLAAHWEKKLTKAHVFECNLEITIQKIISPKVKIALRTSGHLLLGVVRIYNRK
  AKYLLADCSEAFLKMKMTFRPGLVDLPKENFEAAYNTITLPEEFHDFEIYNINEIDISEPLAQNQSRPEE
  ITLREEYSNDLLFQAGSFGDEPEILRRHSFFDDNILMNSSGLVVEHSSGSFAEEKSLFFDNGDGFGDEGA
  AGEMIDNLLQDESTFLEEAYLNKEVSLPPELPSSIMVEPGNSDDQCIPEDEEINEITLLSNEDEGFTLDP
  IDDLDIADRRRRKKRRLLVDPVKEISSKAMHRQLASFMDTLMVLDLAPPTQRLMMWKKRGGVDMLLSTAT
  QDLINDELKMLFTKCFLSSDYKLAKLTLKESVRKEVGNQQIAEPSVMGEPNSHSELDQPQDWKDVTDESV
  GSFQENVNMNVNSEQDILGMISPAVEGLSSMNGSLAQENCPAELESSGSKQNTEAEKWNQRLFQTLNVLR
  EFNKMGMQSFSLKKLCRNSDRKQAAAKFYTLLILKKHRAIELSQSVPYADIIATVGPMFYKM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001276400
• ORF Size: 1656 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276400.1, NP_001263329.1
• RefSeq Size: 1659 bp
• RefSeq ORF: 1659 bp
• Locus ID: 668929
• UniProt ID: A2AU37
• Cytogenetics: 2
• MW: 63.1 kDa
• Gene Summary: Meiosis-specific component of some cohesin complex required during the initial steps of prophase I in male meiosis. Probably required during early meiosis in males for separation of sister chromatids and homologous chromosomes. Replaces RAD21 in premeiotic S phase (during early stages of prophase I), while RAD21 reappears in later stages of prophase I. Involved in synaptonemal complex assembly, synapsis initiation and crossover recombination between homologous chromosomes during prophase I. Not required for meiosis in females in young mice, while it is required later as mice age.[UniProtKB/Swiss-Prot Function]