Poli (NM_001289515) Mouse Tagged ORF Clone

CAT#: MR230470

Poli (myc-DDK-tagged) - Mouse polymerase (DNA directed), iota (Poli), transcript variant 3

Product Images

Circular map for MR230470

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Poli
• Synonyms: Rad30b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230470 representing NM_001289515
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCGGAGACTTGTGGTTGGATCGCAGATTGCAGCAGAGATGCGGGAAGCCATGTATAATCAGCTCG
  GGCTCACAGGCTGCGCCGGAGTGGCTCCTAATAAACTCTTGGCAAAGTTAGTGTCTGGGGTTTTTAAACC
  AAATCAGCAGACGGTCTTACTACCTGAAAGTTGTCAACATCTCATTCACAGTTTGAACCACATAAAGGAA
  ATTCCTGGTATCGGCTATAAAACTGCCAAGCGTCTCGAAGTTCTGGGAATCAATAGTGTTCATGATCTCC
  AAACCTTTCCAATCAAAACATTAGAAAAAGAATTAGGAATTGCAATTGCTCAGCGTATCCAGCAGCTCAG
  TTTTGGAGAGGACAAGTCTCCCGTCACACCGTCAGGGCCACCACAGTCCTTTAGTGAAGAAGATACGTTT
  AAAAAGTGTTCCTCAGAAGTGGAAGCTAAAGCTAAGATTGAAGAATTACTTTCCAGCCTTTTGACCAGAG
  TATGCCAGGATGGAAGGAAGCCCCATACAGTAAGATTAGTCATCCGTCGGTACTCTGACAAACACTGTAA
  TCGAGAGAGTCGTCAGTGCCCTATCCCATCCCACGTCATTCAGAAGTTAGGGACAGGAAATCATGACTCC
  ATGCCTCCCCTGATTGATATCCTTATGAAACTTTTCCGAAATATGGTGAACGTGAAGATGCCCTTTCACC
  TGACTCTTATGAGCGTGTGCTTCTGCAACCTGAAAGCCCTGAGCAGTGCTAAGAAAGGGCCTATGGACTG
  CTACCTAACGTCCCTCTCAACCCCTGCCTACACCGACAAGCGCGCCTTTAAAGTGAAGGACACCCACACG
  GAAGACTCTCACAAGGAGAAAGAAGCAAACTGGGATTGTCTACCAAGTAGAAGAATCGAAAGCACAGGAA
  CGGGGGAGTCTCCGTTGGATGCCACCTGTTTTCCTAAAGAAAAAGACACAAGTGACTTGCCCCTCCAGGC
  ACTGCCAGAGGGTGTTGATCAAGAAGTCTTTAAGCAACTTCCAGCAGATATTCAAGAAGAAATCCTTTCT
  GGAAAATCTAGAGAAAATCTAAAAGGGAAAGGAAGTTTAAGTTGTCCGCTGCATGCCTCTAGAGGAGTAT
  TGTCTTTCTTTTCTACAAAGCAAATGCAAGCCAGTCGCTTAAGCCCCAGAGATACTGCGCTCCCTAGCAA
  GCGGGTATCAGCTGCATCTCCCTGTGAGCCGGGAACGTCAGGACTGAGCCCCGGGAGCACCTCCCATCCA
  TCGTGCGGAAAGGACTGTTCCTATTACATAGACAGCCAGTTAAAGGATGAGCAAACGAGTCAAGGCCCTA
  CTGAGTCTCAAGGATGCCAGTTTTCCAGCACGAACCCTGCTGTTTCTGGTTTCCATTCCTTTCCTAATCT
  GCAGACTGAACAGCTCTTCTCCACACATCGCACTGTAGACAGCCACAAGCAGACAGCCACCGCCTCTCAC
  CAGGGACTAGAAAGTCACCAGGGACTAGAAAGCAGAGAGCTGGATTCTGCGGAAGAAAAGCTTCCTTTCC
  CACCAGACATTGATCCGCAAGTTTTCTATGAGCTTCCAGAAGAGGTCCAAAAGGAACTGATGGCGGAATG
  GGAGAGAGCTGGAGCTGCGCGCCCCTCGGCGCACAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230470 representing NM_001289515
  Red=Cloning site Green=Tags(s)
  
  MHRRLVVGSQIAAEMREAMYNQLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNHIKE
  IPGIGYKTAKRLEVLGINSVHDLQTFPIKTLEKELGIAIAQRIQQLSFGEDKSPVTPSGPPQSFSEEDTF
  KKCSSEVEAKAKIEELLSSLLTRVCQDGRKPHTVRLVIRRYSDKHCNRESRQCPIPSHVIQKLGTGNHDS
  MPPLIDILMKLFRNMVNVKMPFHLTLMSVCFCNLKALSSAKKGPMDCYLTSLSTPAYTDKRAFKVKDTHT
  EDSHKEKEANWDCLPSRRIESTGTGESPLDATCFPKEKDTSDLPLQALPEGVDQEVFKQLPADIQEEILS
  GKSRENLKGKGSLSCPLHASRGVLSFFSTKQMQASRLSPRDTALPSKRVSAASPCEPGTSGLSPGSTSHP
  SCGKDCSYYIDSQLKDEQTSQGPTESQGCQFSSTNPAVSGFHSFPNLQTEQLFSTHRTVDSHKQTATASH
  QGLESHQGLESRELDSAEEKLPFPPDIDPQVFYELPEEVQKELMAEWERAGAARPSAHR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289515
• ORF Size: 1647 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289515.1, NP_001276444.1
• RefSeq Size: 3582 bp
• RefSeq ORF: 1650 bp
• Locus ID: 26447
• UniProt ID: Q6R3M4
• Cytogenetics: 18 E2
• MW: 61.1 kDa
• Gene Summary: Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity (By similarity).[UniProtKB/Swiss-Prot Function]