Cbs (NM_001271353) Mouse Tagged ORF Clone

CAT#: MR230463

Cbs (myc-DDK-tagged) - Mouse cystathionine beta-synthase (Cbs), transcript variant 3

Product Images

Circular map for MR230463

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cbs
• Synonyms: AI047524; AI303044; HIP4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230463 representing NM_001271353
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTTCAGGGACATCCCAGTGTGAAGATGGCTCTGCTGGGGGCTTCCAGCACTTGGACATGCACTCAG
  AAAAGAGACAACTGGAGAAGGGCCCCTCAGGGGACAAGGATCGAGTCTGGATCCGGCCTGATACCCCAAG
  CAGATGTACCTGGCAGCTGGGCAGGGCCATGGCGGACTCCCCACATTATCACACAGTGCTGACCAAATCC
  CCCAAAATTTTACCAGATATTCTGAGGAAAATTGGGAACACCCCTATGGTCAGAATCAACAAGATCTCAA
  AGAATGCCGGTCTCAAGTGTGAGCTCTTGGCCAAGTGTGAGTTCTTCAATGCGGGTGGGAGTGTGAAGGA
  CCGCATCAGCCTTCGGATGATCGAAGATGCTGAGCGAGCTGGAAACTTGAAGCCTGGAGACACTATCATT
  GAGCCAACTTCTGGCAACACAGGGATCGGGCTGGCTCTGGCTGCTGCAGTGAAGGGCTATCGCTGCATTA
  TCGTGATGCCGGAGAAGATGAGTATGGAGAAGGTGGATGTGCTGCGGGCTCTGGGAGCCGAGATTGTGAG
  GACGCCCACCAATGCCAGATTTGATTCCCCCGAGTCCCACGTGGGAGTGGCATGGCGACTGAAGAACGAA
  ATCCCTAATTCTCACATTCTGGACCAGTACCGCAATGCCAGCAACCCTTTGGCACACTACGATGACACCG
  CCGAGGAGATCCTGCAGCAGTGTGACGGGAAGCTGGATATGCTGGTGGCTTCAGCAGGCACGGGTGGCAC
  CATCACAGGGATCGCCAGAAAGCTGAAGGAGAAGTGCCCTGGCTGTAAAATCATCGGTGTCGATCCTGAA
  GGCTCCATCCTTGCGGAGCCCGAGGAGCTGAACCAGACGGAGCAAACAGCCTATGAGGTGGAAGGGATTG
  GCTACGACTTCATCCCGACAGTCCTGGACAGGGCGGTGGTGGATAAGTGGTTCAAGAGCAACGATGAAGA
  TTCCTTCGCCTTTGCCCGCATGCTCATCGCACAGGAAGGACTGCTATGTGGTGGAAGCTCTGGCAGCGCC
  ATGGCTGTGGCTGTGAAGGCTGCCCGGGAGCTGCAGGAAGGGCAGCGCTGTGTGGTCATCCTGCCTGACT
  CTGTGCGGAACTACATGTCCAAGTTCCTGAGTGACAAATGGATGCTGCAGAAAGGTTTCATGAAAGAGGA
  GCTCTCAGTGAAGAGGCCCTGGTGGTGGCGTCTGCGTGTTCAAGAGCTGAGCCTGTCGGCCCCGCTGACC
  GTGTTGCCCACGGTCACCTGTGAGGACACCATCGCCATCCTCCGGGAGAAGGGTTTTGACCAGGCACCTG
  TGGTCAACGAGTCTGGGGCCATCCTAGGGATGGTGACCCTCGGGAACATGCTGTCATCCCTGCTGGCTGG
  AAAGGTGCGGCCATCAGACGAAGTCTGCAAAGTCCTCTACAAGCAGTTCAAACCGATCCACCTGACCGAC
  ACGCTGGGCACACTCTCTCACATCCTGGAGATGGACCACTTCGCCCTGGTGGTCCACGAGCAGATCCAAT
  ACTGCAGCAATGGCATGTCCAGCAAGCAGCAGATGGTGTTTGGGGTTGTCACTGCCATTGACCTGCTAAA
  CTTCGTGGCAGCCCGTGAGCAGACCCAGACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230463 representing NM_001271353
  Red=Cloning site Green=Tags(s)
  
  MPSGTSQCEDGSAGGFQHLDMHSEKRQLEKGPSGDKDRVWIRPDTPSRCTWQLGRAMADSPHYHTVLTKS
  PKILPDILRKIGNTPMVRINKISKNAGLKCELLAKCEFFNAGGSVKDRISLRMIEDAERAGNLKPGDTII
  EPTSGNTGIGLALAAAVKGYRCIIVMPEKMSMEKVDVLRALGAEIVRTPTNARFDSPESHVGVAWRLKNE
  IPNSHILDQYRNASNPLAHYDDTAEEILQQCDGKLDMLVASAGTGGTITGIARKLKEKCPGCKIIGVDPE
  GSILAEPEELNQTEQTAYEVEGIGYDFIPTVLDRAVVDKWFKSNDEDSFAFARMLIAQEGLLCGGSSGSA
  MAVAVKAARELQEGQRCVVILPDSVRNYMSKFLSDKWMLQKGFMKEELSVKRPWWWRLRVQELSLSAPLT
  VLPTVTCEDTIAILREKGFDQAPVVNESGAILGMVTLGNMLSSLLAGKVRPSDEVCKVLYKQFKPIHLTD
  TLGTLSHILEMDHFALVVHEQIQYCSNGMSSKQQMVFGVVTAIDLLNFVAAREQTQT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271353
• ORF Size: 1641 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271353.1, NP_001258282.1
• RefSeq Size: 2458 bp
• RefSeq ORF: 1644 bp
• Locus ID: 12411
• UniProt ID: Q91WT9
• Cytogenetics: 17 16.93 cM
• MW: 60.6 kDa
• Gene Summary: Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine (By similarity). Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons (By similarity).[UniProtKB/Swiss-Prot Function]