Cbs (NM_001271353) Mouse Untagged Clone

CAT#: MC228252

Cbs (untagged) - Mouse cystathionine beta-synthase (Cbs), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cbs
• Synonyms: AI047524; AI303044; HIP4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228252 representing NM_001271353
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTTCAGGGACATCCCAGTGTGAAGATGGCTCTGCTGGGGGCTTCCAGCACTTGGACATGCACTCAG
  AAAAGAGACAACTGGAGAAGGGCCCCTCAGGGGACAAGGATCGAGTCTGGATCCGGCCTGATACCCCAAG
  CAGATGTACCTGGCAGCTGGGCAGGGCCATGGCGGACTCCCCACATTATCACACAGTGCTGACCAAATCC
  CCCAAAATTTTACCAGATATTCTGAGGAAAATTGGGAACACCCCTATGGTCAGAATCAACAAGATCTCAA
  AGAATGCCGGTCTCAAGTGTGAGCTCTTGGCCAAGTGTGAGTTCTTCAATGCGGGTGGGAGTGTGAAGGA
  CCGCATCAGCCTTCGGATGATCGAAGATGCTGAGCGAGCTGGAAACTTGAAGCCTGGAGACACTATCATT
  GAGCCAACTTCTGGCAACACAGGGATCGGGCTGGCTCTGGCTGCTGCAGTGAAGGGCTATCGCTGCATTA
  TCGTGATGCCGGAGAAGATGAGTATGGAGAAGGTGGATGTGCTGCGGGCTCTGGGAGCCGAGATTGTGAG
  GACGCCCACCAATGCCAGATTTGATTCCCCCGAGTCCCACGTGGGAGTGGCATGGCGACTGAAGAACGAA
  ATCCCTAATTCTCACATTCTGGACCAGTACCGCAATGCCAGCAACCCTTTGGCACACTACGATGACACCG
  CCGAGGAGATCCTGCAGCAGTGTGACGGGAAGCTGGATATGCTGGTGGCTTCAGCAGGCACGGGTGGCAC
  CATCACAGGGATCGCCAGAAAGCTGAAGGAGAAGTGCCCTGGCTGTAAAATCATCGGTGTCGATCCTGAA
  GGCTCCATCCTTGCGGAGCCCGAGGAGCTGAACCAGACGGAGCAAACAGCCTATGAGGTGGAAGGGATTG
  GCTACGACTTCATCCCGACAGTCCTGGACAGGGCGGTGGTGGATAAGTGGTTCAAGAGCAACGATGAAGA
  TTCCTTCGCCTTTGCCCGCATGCTCATCGCACAGGAAGGACTGCTATGTGGTGGAAGCTCTGGCAGCGCC
  ATGGCTGTGGCTGTGAAGGCTGCCCGGGAGCTGCAGGAAGGGCAGCGCTGTGTGGTCATCCTGCCTGACT
  CTGTGCGGAACTACATGTCCAAGTTCCTGAGTGACAAATGGATGCTGCAGAAAGGTTTCATGAAAGAGGA
  GCTCTCAGTGAAGAGGCCCTGGTGGTGGCGTCTGCGTGTTCAAGAGCTGAGCCTGTCGGCCCCGCTGACC
  GTGTTGCCCACGGTCACCTGTGAGGACACCATCGCCATCCTCCGGGAGAAGGGTTTTGACCAGGCACCTG
  TGGTCAACGAGTCTGGGGCCATCCTAGGGATGGTGACCCTCGGGAACATGCTGTCATCCCTGCTGGCTGG
  AAAGGTGCGGCCATCAGACGAAGTCTGCAAAGTCCTCTACAAGCAGTTCAAACCGATCCACCTGACCGAC
  ACGCTGGGCACACTCTCTCACATCCTGGAGATGGACCACTTCGCCCTGGTGGTCCACGAGCAGATCCAAT
  ACTGCAGCAATGGCATGTCCAGCAAGCAGCAGATGGTGTTTGGGGTTGTCACTGCCATTGACCTGCTAAA
  CTTCGTGGCAGCCCGTGAGCAGACCCAGACATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001271353
• Insert Size: 1644 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271353.1, NP_001258282.1
• RefSeq Size: 2458 bp
• RefSeq ORF: 1644 bp
• Locus ID: 12411
• UniProt ID: Q91WT9
• Cytogenetics: 17 16.93 cM
• Gene Summary: Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine (By similarity). Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses an alternate splice site in the 5' UTR and lacks an alternate in-frame exon in the 3' coding region compared to variant 1. Both variants 2 and 3 encode the same isoform (2), which is shorter than isoform 1.