Slc37a1 (NM_001242427) Mouse Tagged ORF Clone

CAT#: MR230405

Slc37a1 (myc-DDK-tagged) - Mouse solute carrier family 37 (glycerol-3-phosphate transporter), member 1 (Slc37a1), transcript variant 1

Product Images

Circular map for MR230405

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc37a1
• Synonyms: G3PP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230405 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGCTTCCCGTGGGCATCCGCTTCATCATCTCCTTCTCCAGGGATCAGTGGTACCGGGCTTTCA
  TTTTTATGCTGACCTTTCTGCTCTATGCAAGCTTCCACTTATCCCGAAAGCCTATCAGCATAGTGAAGGG
  CGAGCTTCACAAGCAGTGTACTGCAGGAGATGGGCCTGAGTCTCCATTCAGTGACCCTAGCAGCAGCACC
  AGGCATGGCCCCACCCACTGGCTCCTGAACAATGAGACCGACTGTGGCTGGGCCCCCTTCGATAAGAACA
  ATTACCAGCAGCTGCTTGGAGCCCTGGACTACGCCTTCCTGTGCGCCTATGCCATTGGAATGTACCTGAG
  TGGAATAATAGGGGAACGCCTGCCGATTAGGTATTACCTGACTTTCGGGATGCTGGCCAGCGGCGCCTTC
  ACAGCCCTGTTCGGCTTGGGCTATTTCTACAACATCCACAGCCTGGGATTCTACGTGGTGACCCAGATCA
  TTAATGGACTGGTGCAGACCACAGGCTGGCCCAGCGTTGTCACCTGCCTCAGCAACTGGTTTGGAAAAGG
  AAGGCGTGGCCTGATCATGGGGATTTGGAACTCCCACACCTCCGTGGGCAACATTCTGGGGTCCTTGATT
  GCCGGCTACTGGGTGTCTACGTGCTGGGGCCTGTCATTTATCGTGCCTGGGGCCATCGTGGCAGCCATGG
  GGATAGTGTGCTTTCTCTTTCTCATCGAACACCCTAAGGACGTCAAGTGCTCCTCCACCCTCCCCACGCA
  CCCTAGAGCCTCTGAGAATGGCATAAACAGATTTAGACTGCAGAAGCAAACCACGTACAGTGAAAAGAAT
  GGACCTCTGGACCCAGAGCTTCAGTGCTTGCTACTCTCCGACGGGAAGAACCCCCTTCACCCAAGCCATA
  TTGTTGTCCTCCCTGCTGATGGCAACATGGCCGCCATCAGCTTCACGGGAGCCCTGAGGATCCCGGGCGT
  GATTGAGTTTTCTCTGTGTTTGCTGTTTGCCAAGCTGGTCAGCTACACATTCCTGTTCTGGCTCCCGCTG
  TACATCACCAGTGTAGATCACCTGGATGCCAAGAAAGCTGGGGAGCTGTCCACATTGTTTGACGTGGGCG
  GGATCTTTGGTGGGATTCTAGCAGGCGTGATCTCCGACCGTCTGGAGAAACGAGCCTCCACTTGTGGTCT
  GATGCTATTGCTGGCAGCACCCACGCTCTATGTCTTCTCCTCCGTCAGCAGAATGGGCCTAGAAGCCACC
  ATAGCCATGTTGCTCCTCAGTGGGGCCCTGGTGAGCGGGCCCTACGCACTGATCACCACTGCCGTCTCTG
  CAGACTTGGGGACTCACAAGAGTCTGAAAGGGAACTCCCACGCCCTGTCCACCGTGACAGCCATCATCGA
  CGGCACTGGGTCTGTAGGAGCTGCCTTGGGGCCCCTGCTGGCAGGGCTCATCTCCCCCTCTGGATGGAGC
  AACGTGTTCTACATGCTGATGTTTGCAGATGCCTGTGCCTTACTGTTCCTGGTCCGCCTCATACACAAGG
  AGTTGAGCTGTCCAGGCCCAGCCGCAGGGATCCAGGCTCCGCTGAAGGAACAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230405 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MALLPVGIRFIISFSRDQWYRAFIFMLTFLLYASFHLSRKPISIVKGELHKQCTAGDGPESPFSDPSSST
  RHGPTHWLLNNETDCGWAPFDKNNYQQLLGALDYAFLCAYAIGMYLSGIIGERLPIRYYLTFGMLASGAF
  TALFGLGYFYNIHSLGFYVVTQIINGLVQTTGWPSVVTCLSNWFGKGRRGLIMGIWNSHTSVGNILGSLI
  AGYWVSTCWGLSFIVPGAIVAAMGIVCFLFLIEHPKDVKCSSTLPTHPRASENGINRFRLQKQTTYSEKN
  GPLDPELQCLLLSDGKNPLHPSHIVVLPADGNMAAISFTGALRIPGVIEFSLCLLFAKLVSYTFLFWLPL
  YITSVDHLDAKKAGELSTLFDVGGIFGGILAGVISDRLEKRASTCGLMLLLAAPTLYVFSSVSRMGLEAT
  IAMLLLSGALVSGPYALITTAVSADLGTHKSLKGNSHALSTVTAIIDGTGSVGAALGPLLAGLISPSGWS
  NVFYMLMFADACALLFLVRLIHKELSCPGPAAGIQAPLKEH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001242427
• ORF Size: 1596 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001242427.1, NP_001229356.1
• RefSeq Size: 3362 bp
• RefSeq ORF: 1596 bp
• Locus ID: 224674
• UniProt ID: Q8R070
• Cytogenetics: 17 B1
• MW: 57.4 kDa
• Gene Summary: Inorganic phosphate and glucose-6-phosphate antiporter. May transport cytoplasmic glucose-6-phosphate into the lumen of the endoplasmic reticulum and translocate inorganic phosphate into the opposite direction. Independent of a lumenal glucose-6-phosphatase. May not play a role in homeostatic regulation of blood glucose levels.[UniProtKB/Swiss-Prot Function]