Irf5 (NM_001252382) Mouse Tagged ORF Clone

CAT#: MR230297

Irf5 (myc-DDK-tagged) - Mouse interferon regulatory factor 5 (Irf5), transcript variant 1

Product Images

Circular map for MR230297

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Irf5
• Synonyms: AW491843; mirf5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230297 representing NM_001252382
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCAAAACCCAAAGCCCTTTGCCATGAACCACTCAGCCCCAGGGATTCCCCCACCACCCCGCCGTG
  TGAGGCTGAAGCCCTGGTTGGTGGCCCAGGTGAACAGCTGCCAGTACCCAGGGCTTCAGTGGGTCAACGG
  GGAAAAGAAACTCTTCTATATACCCTGGCGCCATGCCACGAGGCATGGTCCCAGCCAGGATGGGGACAAC
  ACCATCTTCAAGGCCTGGGCTAAAGAGACAGGGAAGTACACTGAAGGGGTGGATGAGGCTGACCCAGCCA
  AGTGGAAGGCCAACCTGCGCTGTGCCCTTAACAAAAGCCGTGACTTCCAGCTGTTCTATGATGGCCCTCG
  GGACATGCCACCTCAGCCGTACAAGATCTACGAGGTCTGCTCCAACGGCCCTGCTCCCACAGAGAGCCAA
  CCCACTGATGATTACGTTCTGGGAGAAGAGGAGGAGGAGGAAGAGGAAGAGCTCCAGAGAATGCTACCAG
  GCCTGAGCATCACAGAGCCTGCGCTACCTGGGCCTCCCAACGCACCCTATTCCTTACCCAAAGAAGACAC
  CAAGTGGCCACCTGCTCTCCAGCCACCTGTAGGGCTGGGTCCCCCTGTCCCAGACCCAAATCTCCTGGCC
  CCTCCCTCTGGAAATCCTGCTGGCTTCAGGCAGCTTCTCCCTGAGGTCCTGGAGCCTGGACCTCTGGCTT
  CCAGCCAGCCCCCTACAGAACCACTCTTGCCTGACCTGCTGATCAGCCCCCACATGTTGCCTTTGACGGA
  CCTAGAGATCAAGTTCCAGTACCGGGGACGCGCACCCCGGACCCTCACCATCAGCAACCCACAAGGCTGC
  AGGCTCTTCTACAGCCAGCTAGAGGCTACCCAGGAGCAAGTGGAACTCTTTGGCCCTGTGACCCTGGAGC
  AAGTGCGCTTCCCTAGCCCAGAGGACATCCCCAGTGACAAGCAGCGTTTCTATACGAACCAGCTGCTAGA
  TGTCCTGGACCGTGGGCTCATCCTGCAGCTGCAGGGCCAGGACCTGTACGCCATCCGTCTGTGCCAGTGT
  AAGGTGTTCTGGAGTGGGCCCTGCGCCTTGGCCCATGGCTCCTGCCCCAACCCCATTCAGCGGGAAGTCA
  AGACGAAGCTCTTTAGCCTAGAGCAGTTTCTCAATGAGCTCATCCTGTTCCAGAAGGGCCAGACTAATAC
  CCCACCACCTTTTGAGATCTTCTTTTGCTTTGGAGAAGAATGGCCTGATGTCAAACCCCGAGAGAAGAAG
  CTCATTACTGTACAGGTGGTACCTGTTGCAGCCCGGTTGCTGCTGGAGATGTTCTCAGGGGAGCTTTCTT
  GGTCGGCAGACAGCATCCGACTGCAGATCTCAAACCCGGATCTCAAAGACCACATGGTAGAGCAGTTTAA
  AGAGCTTCATCACCTCTGGCAGTCCCAGCAGCAATTGCAGCCCATGGTCCAGGCCCCTCCTGTGGCAGGC
  CTCGATGCAAGCCAGGGGCCCTGGCCCATGCACCCAGTTGGCATGCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230297 representing NM_001252382
  Red=Cloning site Green=Tags(s)
  
  MLQNPKPFAMNHSAPGIPPPPRRVRLKPWLVAQVNSCQYPGLQWVNGEKKLFYIPWRHATRHGPSQDGDN
  TIFKAWAKETGKYTEGVDEADPAKWKANLRCALNKSRDFQLFYDGPRDMPPQPYKIYEVCSNGPAPTESQ
  PTDDYVLGEEEEEEEEELQRMLPGLSITEPALPGPPNAPYSLPKEDTKWPPALQPPVGLGPPVPDPNLLA
  PPSGNPAGFRQLLPEVLEPGPLASSQPPTEPLLPDLLISPHMLPLTDLEIKFQYRGRAPRTLTISNPQGC
  RLFYSQLEATQEQVELFGPVTLEQVRFPSPEDIPSDKQRFYTNQLLDVLDRGLILQLQGQDLYAIRLCQC
  KVFWSGPCALAHGSCPNPIQREVKTKLFSLEQFLNELILFQKGQTNTPPPFEIFFCFGEEWPDVKPREKK
  LITVQVVPVAARLLLEMFSGELSWSADSIRLQISNPDLKDHMVEQFKELHHLWQSQQQLQPMVQAPPVAG
  LDASQGPWPMHPVGMQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001252382
• ORF Size: 1518 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252382.1, NP_001239311.1
• RefSeq Size: 2258 bp
• RefSeq ORF: 1521 bp
• Locus ID: 27056
• UniProt ID: P56477
• Cytogenetics: 6 12.36 cM
• MW: 57.5 kDa
• Gene Summary: Transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. Activated by TLR7 or TLR8 signaling (By similarity).[UniProtKB/Swiss-Prot Function]