Angpt1 (NM_001286062) Mouse Tagged ORF Clone

CAT#: MR230243

Angpt1 (myc-DDK-tagged) - Mouse angiopoietin 1 (Angpt1), transcript variant 2

Product Images

Circular map for MR230243

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Angpt1
• Synonyms: 1110046O21Rik; an; Ang; Ang-1; Ang1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230243 representing NM_001286062
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGTTTTCCTTTCCTTTGCATTCTTCGCTGCCATTCTGACTCACATAGGGTGCAGCAACCAGCGCC
  GAAATCCAGAAAACGGAGGGAGAAGATATAACCGGATTCAACATGGGCAATGTGCCTACACTTTCATTCT
  TCCAGAACACGACGGGAACTGCCGTGAGAGTGCGACAGAGCAGTACAACACCAACGCTCTGCAAAGGGAT
  GCTCCACACGTGGAGCCGGATTTCTCTTCCCAGAAACTTCAGCATCTGGAGCATGTGATGGAAAATTATA
  CTCAGTGGCTGCAAAAACTTGAGAATTACATTGTGGAAAATATGAAGTCGGAGATGGCCCAGATACAACA
  GAATGCTGTTCAAAACCACACGGCCACCATGCTTGAGATAGGAACCAGTCTCTTATCTCAGACTGCAGAG
  CAGACCCGAAAGCTGACAGATGTTGAGACCCAGGTACTAAATCAAACATCCCGACTTGAAATACAACTGC
  TAGAGAATTCATTATCAACATACAAGCTAGAGAAGCAACTTCTCCAACAGACAAATGAAATTCTGAAGAT
  TCACGAAAAAAACAGTTTACTAGAGCACAAAATCTTAGAAATGGAGGGAAAACACAAAGAAGAATTGGAC
  ACCTTGAAGGAGGAGAAAGAAAACCTTCAAGGCTTGGTTTCTCGTCAGACATTCATCATCCAGGAGTTGG
  AGAAGCAACTTAGTAGAGCTACCAACAACAACAGCATCCTGCAGAAGCAACAACTGGAGCTCATGGACAC
  AGTTCATAACCTTATCAGCCTTTGCACTAAAGAAGTTTTGCTAAAGGGAGGAAAAAGAGAAGAAGAGAAA
  CCATTTCGAGACTGTGCAGATGTATATCAAGCTGGTTTTAATAAAAGTGGAATCTACACTATTTATTTTA
  ATAATATGCCAGAACCCAAAAAGGTATTTTGCAATATGGATGTGAATGGGGGAGGTTGGACAGTAATACA
  ACACCGGGAAGATGGAAGCCTGGATTTCCAGAGGGGCTGGAAGGAGTATAAAATGGGTTTTGGGAATCCC
  TCTGGTGAATATTGGCTTGGGAACGAGTTCATTTTTGCAATAACCAGTCAGAGGCAGTACATGCTGAGGA
  TTGAGCTGATGGACTGGGAAGGGAACCGAGCCTACTCACAGTACGACAGATTCCACATAGGAAATGAAAA
  GCAGAACTATAGGTTATATTTAAAAGGTCACACAGGGACAGCAGGCAAACAGAGCAGCTTGATCTTACAC
  GGTGCCGATTTCAGCACGAAGGATGCTGATAACGACAACTGTATGTGCAAATGCGCTCTCATGCTAACAG
  GAGGTTGGTGGTTCGATGCCTGTGGCCCTTCCAATCTAAATGGAATGTTCTACACTGCGGGACAAAATCA
  TGGAAAACTGAATGGGATAAAGTGGCACTACTTCAAAGGGCCCAGTTACTCCTTACGTTCCACCACCATG
  ATGATCCGGCCCTTGGACTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230243 representing NM_001286062
  Red=Cloning site Green=Tags(s)
  
  MTVFLSFAFFAAILTHIGCSNQRRNPENGGRRYNRIQHGQCAYTFILPEHDGNCRESATEQYNTNALQRD
  APHVEPDFSSQKLQHLEHVMENYTQWLQKLENYIVENMKSEMAQIQQNAVQNHTATMLEIGTSLLSQTAE
  QTRKLTDVETQVLNQTSRLEIQLLENSLSTYKLEKQLLQQTNEILKIHEKNSLLEHKILEMEGKHKEELD
  TLKEEKENLQGLVSRQTFIIQELEKQLSRATNNNSILQKQQLELMDTVHNLISLCTKEVLLKGGKREEEK
  PFRDCADVYQAGFNKSGIYTIYFNNMPEPKKVFCNMDVNGGGWTVIQHREDGSLDFQRGWKEYKMGFGNP
  SGEYWLGNEFIFAITSQRQYMLRIELMDWEGNRAYSQYDRFHIGNEKQNYRLYLKGHTGTAGKQSSLILH
  GADFSTKDADNDNCMCKCALMLTGGWWFDACGPSNLNGMFYTAGQNHGKLNGIKWHYFKGPSYSLRSTTM
  MIRPLDF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286062
• ORF Size: 1491 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286062.1, NP_001272991.1
• RefSeq Size: 4313 bp
• RefSeq ORF: 1494 bp
• Locus ID: 11600
• Cytogenetics: 15 16.69 cM
• MW: 57.9 kDa
• Gene Summary: This gene encodes a secreted glycoprotein that belongs to the angiopoietin family of vascular growth factors. The encoded protein is a ligand in the vascular tyrosine kinase signaling pathway and regulates the formation and stabilization of blood vessels. This protein also functions in striated muscles by promoting proliferation, migration and differentiation of skeletal myoblasts and plays an essential role in the vascular response to tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013]