Mdm4 (NM_001302803) Mouse Tagged ORF Clone

CAT#: MR230179

Mdm4 (myc-DDK-tagged) - Mouse transformed mouse 3T3 cell double minute 4 (Mdm4), transcript variant 4

Product Images

Circular map for MR230179

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mdm4
• Synonyms: 4933417N07Rik; AA414968; AL023055; AU018793; AU021806; C85810; Mdmx
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230179 representing NM_001302803
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACATCACATTCCACCTCGGCCCAGTGTTCAGCATCTGACAGTGCTTGCAGAATTTCTTCGGAACAAA
  TTAGTCAGGTGCGGCCAAAACTGCAGCTTTTGAAGATTTTGCATGCAGCAGGTGCGCAGGGGGAAGTATT
  CACCATGAAAGAGGTAATGCACTATCTAGGCCAGTATATAATGGTGAAGCAGCTCTATGATCAACAGGAG
  CAACATATGGTATACTGTGGTGGAGATCTTTTGGGAGATCTACTTGGATGTCAGAGCTTTTCTGTGAAAG
  ATCCAAGCCCTCTCTATGACATGCTAAGAAAGAATCTTGTTACATCAGCTTCTATTAACACAGATGCTGC
  TCAGACTCTCGCTCTCGCACAGGATCACACTATGGATTTTCCAAGTCAAGACCGACTGAAGCACGGTGCA
  ACAGAATACTCCAATCCCAGAAAAAGAACTGAAGAAGAGGATACTCACACACTGCCTACCTCACGACATA
  AATGCAGAGACTCCAGAGCAGATGAAGACTTGATAGAACATTTATCTCAAGATGAGACATCTAGGCTTGA
  CCTTGATTTTGAGGAGTGGGACGTTGCTGGCCTGCCTTGGTGGTTTCTAGGGAATTTGAGAAACAACTGT
  ATTCCTAAAAGTAATGGCTCAACTGATTTACAGACAAATCAGGATATAGGTACTGCCATTGTTTCAGACA
  CTACGGATGATTTGTGGTTTTTAAATGAGACCGTGTCAGAGCAATTAGGTGTTGGAATAAAAGTTGAAGC
  TGCTAATTCTGAGCAAACAAGTGAAGTAGGGAAAACAAGTAACAAGAAGACGGTGGAGGTGGGAAAGGAT
  GATGATCTTGAGGACTCCAGGTCCTTGAGCGATGATACTGACGTGGAACTTACCTCTGAGGATGAGTGGC
  AGTGTACGGAATGCAAGAAGTTTAATTCTCCAAGCAAGAGGTACTGTTTTCGTTGCTGGGCCTTGAGAAA
  GGATTGGTATTCGGATTGTTCTAAATTAACTCATTCCCTATCTACATCTAATATTACTGCCATACCTGAA
  AAGAAGGACAATGAAGGAATTGATGTTCCCGATTGTAGGAGAACCATTTCAGCTCCTGTTGTTAGGCCTA
  AAGATGGATATTTAAAGGAGGAAAAGCCCAGGTTTGACCCTTGCAACTCAGTGGGATTTTTGGATTTGGC
  TCATAGTTCTGAAAGCCAGGAGATCATCTCAAGCGCGAGAGAACAAACAGATATTTTTTCTGAGCAGAAA
  GCTGAAACAGAAAGTATGGAAGATTTCCAGAATGTCTTGAAGCCGTGTAGCTTATGTGAAAAAAGGCCTC
  GGGATGGGAACATTATTCATGGGAAGACGAGCCATCTGACGACATGTTTCCACTGTGCCAGGAGACTGAA
  GAAGTCTGGGGCTTCGTGTCCTGCTTGTAAGAAAGAGATTCAGTTGGTTATTAAAGTTTTTATAGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230179 representing NM_001302803
  Red=Cloning site Green=Tags(s)
  
  MTSHSTSAQCSASDSACRISSEQISQVRPKLQLLKILHAAGAQGEVFTMKEVMHYLGQYIMVKQLYDQQE
  QHMVYCGGDLLGDLLGCQSFSVKDPSPLYDMLRKNLVTSASINTDAAQTLALAQDHTMDFPSQDRLKHGA
  TEYSNPRKRTEEEDTHTLPTSRHKCRDSRADEDLIEHLSQDETSRLDLDFEEWDVAGLPWWFLGNLRNNC
  IPKSNGSTDLQTNQDIGTAIVSDTTDDLWFLNETVSEQLGVGIKVEAANSEQTSEVGKTSNKKTVEVGKD
  DDLEDSRSLSDDTDVELTSEDEWQCTECKKFNSPSKRYCFRCWALRKDWYSDCSKLTHSLSTSNITAIPE
  KKDNEGIDVPDCRRTISAPVVRPKDGYLKEEKPRFDPCNSVGFLDLAHSSESQEIISSAREQTDIFSEQK
  AETESMEDFQNVLKPCSLCEKRPRDGNIIHGKTSHLTTCFHCARRLKKSGASCPACKKEIQLVIKVFIA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001302803
• ORF Size: 1467 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001302803.1, NP_001289732.1
• RefSeq Size: 7220 bp
• RefSeq ORF: 1470 bp
• Locus ID: 17248
• UniProt ID: O35618
• Cytogenetics: 1 57.75 cM
• MW: 55 kDa
• Gene Summary: This gene encodes a protein that has been shown to negatively regulate the activity of the tumor suppressor protein p53. Homozygous knockout mice exhibit embryonic lethality as a result of p53-dependent apoptosis and cell cycle arrest. Amplification of this gene or overexpression of the encoded protein has been linked to a range of human cancers. A pseudogene has been identified on the X chromosome. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2014]